(Linear) (Six-base) MAPSORT of: KIBB3452.seq  Check: 6965  from: 1  to: 651



KIBB3452.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AfeI AGC'GCT

Cuts at:      0     142     651 
   Size:        142     509
 
AflII C'TTAA_G

Cuts at:      0     163     651 
   Size:        163     488
 
AvaI C'yCGr_G

Cuts at:      0     147     651 
   Size:        147     504
 
BanI G'GyrC_C

Cuts at:      0     151     651 
   Size:        151     500
 
BclI T'GATC_A

Cuts at:      0     347     651 
   Size:        347     304
 
Bme1580I G_kGCm'C

Cuts at:      0     154     651 
   Size:        154     497
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     651 
   Size:        106     545
 
Bpu10I CC'TnA_GC

Cuts at:      0     230     651 
   Size:        230     421
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     565     651 
   Size:        565      86
 
BsaAI yAC'GTr

Cuts at:      0     513     651 
   Size:        513     138
 
BseYI C'CCAG_C

Cuts at:      0     638     651 
   Size:        638      13
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      37     651 
   Size:         37     614
 
BsiHKAI G_wGCw'C

Cuts at:      0     625     651 
   Size:        625      26
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      82     651 
   Size:         82     569
 
Bsp1286I G_dGCh'C

Cuts at:      0     154     625     651 
   Size:        154     471      26

  Fragments arranged by size: 

                 471     154      26
 
BsrDI GCAATG_nn'

Cuts at:      0     574     651 
   Size:        574      77
 
BtgI C'CryG_G

Cuts at:      0     236     626     651 
   Size:        236     390      25

  Fragments arranged by size: 

                 390     236      25
 
DraI TTT'AAA

Cuts at:      0     383     651 
   Size:        383     268
 
EaeI y'GGCC_r

Cuts at:      0     308     434     651 
   Size:        308     126     217

  Fragments arranged by size: 

                 308     217     126
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     106     651 
   Size:        106     545
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      31     651 
   Size:         31     620
 
EcoRV GAT'ATC

Cuts at:      0     243     651 
   Size:        243     408
 
HaeII r_GCGC'y

Cuts at:      0      75     144     268     645     651 
   Size:         75      69     124     377       6

  Fragments arranged by size: 

                 377     124      75      69       6
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     439     471     651 
   Size:        439      32     180

  Fragments arranged by size: 

                 439     180      32
 
HincII GTy'rAC

Cuts at:      0     561     651 
   Size:        561      90
 
MscI TGG'CCA

Cuts at:      0     310     436     651 
   Size:        310     126     215

  Fragments arranged by size: 

                 310     215     126
 
NcoI C'CATG_G

Cuts at:      0     626     651 
   Size:        626      25
 
NruI TCG'CGA

Cuts at:      0      20     651 
   Size:         20     631
 
PfoI T'CCnGG_A

Cuts at:      0      83     651 
   Size:         83     568
 
PmlI CAC'GTG

Cuts at:      0     513     651 
   Size:        513     138
 
SfcI C'TryA_G

Cuts at:      0     497     651 
   Size:        497     154
 
SmlI C'TyrA_G

Cuts at:      0     163     580     651 
   Size:        163     417      71

  Fragments arranged by size: 

                 417     163      71
 
StyI C'CwwG_G

Cuts at:      0     626     651 
   Size:        626      25
 

 Enzymes that do cut:

     AfeI    AflII     AvaI     BanI     BclI Bme1580I     BpmI   Bpu10I
    BpuEI    BsaAI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BsrDI
     BtgI     DraI     EaeI  Eco57MI    EcoNI    EcoRV    HaeII    Hin4I
   HincII     MscI     NcoI     NruI     PfoI     PmlI     SfcI     SmlI
     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
     BbsI    BbvCI     BcgI    BciVI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmrI     BmtI     BplI     BsaI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII
    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     BtsI     ClaI    CspCI
   DraIII     DrdI     EagI     EarI     EciI  EcoICRI EcoO109I    EcoRI
     FalI     FseI     FspI    FspAI  HindIII     HpaI     KasI     KpnI
     MfeI     MluI     MmeI     MslI   MspA1I     NaeI     NarI     NdeI
   NgoMIV     NheI     NotI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PmeI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI
    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI     TstI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI