(Linear) (Six-base) MAPSORT of: KIBB3461.seq  Check: 8530  from: 1  to: 645



KIBB3461.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:01  ..

AccI GT'mk_AC

Cuts at:      0     596     645 
   Size:        596      49
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     645 
   Size:        337     308
 
ApoI r'AATT_y

Cuts at:      0     139     573     645 
   Size:        139     434      72

  Fragments arranged by size: 

                 434     139      72
 
BciVI GTATCCnnnnn_n'

Cuts at:      0     216     645 
   Size:        216     429
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     287     328     605     645 
   Size:        287      41     277      40

  Fragments arranged by size: 

                 287     277      41      40
 
BsiHKAI G_wGCw'C

Cuts at:      0     279     645 
   Size:        279     366
 
Bsp1286I G_dGCh'C

Cuts at:      0     279     645 
   Size:        279     366
 
BspHI T'CATG_A

Cuts at:      0     135     645 
   Size:        135     510
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     577     645 
   Size:        577      68
 
EarI CTCTTCn'nnn_

Cuts at:      0     582     645 
   Size:        582      63
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     337     645 
   Size:        337     308
 
EcoRV GAT'ATC

Cuts at:      0     303     645 
   Size:        303     342
 
MspA1I CmG'CkG

Cuts at:      0      88     229     645 
   Size:         88     141     416

  Fragments arranged by size: 

                 416     141      88
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      40     645 
   Size:         40     605
 
PfoI T'CCnGG_A

Cuts at:      0     434     564     645 
   Size:        434     130      81

  Fragments arranged by size: 

                 434     130      81
 
PvuII CAG'CTG

Cuts at:      0     229     645 
   Size:        229     416
 
ScaI AGT'ACT

Cuts at:      0      71     645 
   Size:         71     574
 
SfcI C'TryA_G

Cuts at:      0     200     267     645 
   Size:        200      67     378

  Fragments arranged by size: 

                 378     200      67
 
SpeI A'CTAG_T

Cuts at:      0      66     645 
   Size:         66     579
 
SspI AAT'ATT

Cuts at:      0     518     645 
   Size:        518     127
 
TatI w'GTAC_w

Cuts at:      0      69     263     645 
   Size:         69     194     382

  Fragments arranged by size: 

                 382     194      69
 

 Enzymes that do cut:

     AccI     AcuI     ApoI    BciVI    BseRI  BsiHKAI Bsp1286I    BspHI
   BstAPI     EarI  Eco57MI    EcoRV   MspA1I    PflMI     PfoI    PvuII
     ScaI     SfcI     SpeI     SspI     TatI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI
     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI     BclI    BfrBI     BglI    BglII
     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I
    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
     BsgI    BsiEI    BsiWI     BsmI    BsmBI    BspEI    BspMI    BsrBI
    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII    BstXI
    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI     ClaI    CspCI
     DraI   DraIII     DrdI     EaeI     EagI     EciI  EcoICRI    EcoNI
 EcoO109I    EcoRI     FalI     FseI     FspI    FspAI    HaeII    Hin4I
   HincII  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MmeI
     MscI     MslI     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI     SapI
     SbfI    SexAI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SphI     SrfI     StuI     StyI     SwaI    TaqII     TstI
  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI     ZraI