Restriction map for
pF1KB3463
(Linear) (Six-base) MAPSORT of: KIBB3463.seq Check: 7856 from: 1 to: 4803
KIBB3463.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
Acc65I G'GTAC_C
Cuts at: 0 3415 4803
Size: 3415 1388
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 2219 2780 3529 3665 3923 4223 4409
Size: 320 1899 561 749 136 258 300 186
Cuts at: 4409 4803
Size: 394
Fragments arranged by size:
1899 749 561 394 320 300 258 186
136
AflII C'TTAA_G
Cuts at: 0 1407 4803
Size: 1407 3396
AflIII A'CryG_T
Cuts at: 0 661 4322 4803
Size: 661 3661 481
Fragments arranged by size:
3661 661 481
AgeI A'CCGG_T
Cuts at: 0 2359 4803
Size: 2359 2444
AhdI GACnn_n'nnGTC
Cuts at: 0 3780 3849 4803
Size: 3780 69 954
Fragments arranged by size:
3780 954 69
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3298 4803
Size: 3298 1505
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2827 2859 4086 4118 4803
Size: 2827 32 1227 32 685
Fragments arranged by size:
2827 1227 685 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 3891 3901 4803
Size: 3891 10 902
Fragments arranged by size:
3891 902 10
ApoI r'AATT_y
Cuts at: 0 395 1008 1133 1501 1787 2347 2462 2673
Size: 395 613 125 368 286 560 115 211
Cuts at: 2673 2750 3222 3429 4803
Size: 77 472 207 1374
Fragments arranged by size:
1374 613 560 472 395 368 286 211
207 125 115 77
AseI AT'TA_AT
Cuts at: 0 142 4803
Size: 142 4661
AvaI C'yCGr_G
Cuts at: 0 52 64 4803
Size: 52 12 4739
Fragments arranged by size:
4739 52 12
BamHI G'GATC_C
Cuts at: 0 1305 4803
Size: 1305 3498
BanI G'GyrC_C
Cuts at: 0 3415 3549 4803
Size: 3415 134 1254
Fragments arranged by size:
3415 1254 134
BanII G_rGCy'C
Cuts at: 0 4229 4803
Size: 4229 574
BbsI GAAGACnn'nnnn_
Cuts at: 0 551 658 1831 2433 3680 4803
Size: 551 107 1173 602 1247 1123
Fragments arranged by size:
1247 1173 1123 602 551 107
BclI T'GATC_A
Cuts at: 0 1247 1725 2247 4803
Size: 1247 478 522 2556
Fragments arranged by size:
2556 1247 522 478
BfrBI ATG'CAT
Cuts at: 0 519 1065 1278 4626 4803
Size: 519 546 213 3348 177
Fragments arranged by size:
3348 546 519 213 177
BglI GCCn_nnn'nGGC
Cuts at: 0 3319 4713 4803
Size: 3319 1394 90
Fragments arranged by size:
3319 1394 90
BglII A'GATC_T
Cuts at: 0 747 2943 4803
Size: 747 2196 1860
Fragments arranged by size:
2196 1860 747
Bme1580I G_kGCm'C
Cuts at: 0 3270 4803
Size: 3270 1533
BmgBI CAC'GTC
Cuts at: 0 664 4803
Size: 664 4139
BmrI ACTGGGnnnn_n'
Cuts at: 0 3920 4414 4803
Size: 3920 494 389
Fragments arranged by size:
3920 494 389
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 963 4803
Size: 963 3840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 835 932 3455 3774 4290 4508 4571 4803
Size: 835 97 2523 319 516 218 63 232
Fragments arranged by size:
2523 835 516 319 232 218 97 63
Bpu10I CC'TnA_GC
Cuts at: 0 1262 2092 4803
Size: 1262 830 2711
Fragments arranged by size:
2711 1262 830
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2519 3710 4031 4803
Size: 2519 1191 321 772
Fragments arranged by size:
2519 1191 772 321
BsaAI yAC'GTr
Cuts at: 0 4325 4803
Size: 4325 478
BsaBI GATnn'nnATC
Cuts at: 0 620 4803
Size: 620 4183
BsaHI Gr'CG_yC
Cuts at: 0 3691 4803
Size: 3691 1112
BsaWI w'CCGG_w
Cuts at: 0 1217 1544 2359 4803
Size: 1217 327 815 2444
Fragments arranged by size:
2444 1217 815 327
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1172 1202 4803
Size: 1172 30 3601
Fragments arranged by size:
3601 1172 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 3385 3713 4293 4511 4803
Size: 3385 328 580 218 292
Fragments arranged by size:
3385 580 328 292 218
BseYI C'CCAG_C
Cuts at: 0 1115 2597 3177 4151 4374 4803
Size: 1115 1482 580 974 223 429
Fragments arranged by size:
1482 1115 974 580 429 223
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 3587 4627 4803
Size: 3587 1040 176
Fragments arranged by size:
3587 1040 176
BsiHKAI G_wGCw'C
Cuts at: 0 159 3255 3935 4372 4803
Size: 159 3096 680 437 431
Fragments arranged by size:
3096 680 437 431 159
BsmI GAATG_Cn'
Cuts at: 0 363 4803
Size: 363 4440
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1273 4803
Size: 1273 3530
Bsp1286I G_dGCh'C
Cuts at: 0 159 3255 3270 3935 4229 4372 4803
Size: 159 3096 15 665 294 143 431
Fragments arranged by size:
3096 665 431 294 159 143 15
BspHI T'CATG_A
Cuts at: 0 2343 4803
Size: 2343 2460
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 786 4803
Size: 786 4017
BsrBI CCG'CTC
Cuts at: 0 57 4803
Size: 57 4746
BsrDI GCAATG_nn'
Cuts at: 0 581 733 4038 4196 4803
Size: 581 152 3305 158 607
Fragments arranged by size:
3305 607 581 158 152
BsrFI r'CCGG_y
Cuts at: 0 2359 4803
Size: 2359 2444
BssSI C'ACGA_G
Cuts at: 0 4672 4803
Size: 4672 131
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1062 4722 4803
Size: 1062 3660 81
Fragments arranged by size:
3660 1062 81
BstBI TT'CG_AA
Cuts at: 0 3153 4803
Size: 3153 1650
BstEII G'GTnAC_C
Cuts at: 0 1471 4803
Size: 1471 3332
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2217 2589 4151 4803
Size: 2217 372 1562 652
Fragments arranged by size:
2217 1562 652 372
BstYI r'GATC_y
Cuts at: 0 238 729 747 1305 2943 4803
Size: 238 491 18 558 1638 1860
Fragments arranged by size:
1860 1638 558 491 238 18
Bsu36I CC'TnA_GG
Cuts at: 0 2405 4803
Size: 2405 2398
BtgI C'CryG_G
Cuts at: 0 11 260 4803
Size: 11 249 4543
Fragments arranged by size:
4543 249 11
BtsI GCAGTG_nn'
Cuts at: 0 4273 4610 4803
Size: 4273 337 193
Fragments arranged by size:
4273 337 193
ClaI AT'CG_AT
Cuts at: 0 2654 4803
Size: 2654 2149
DraI TTT'AAA
Cuts at: 0 965 1139 4803
Size: 965 174 3664
Fragments arranged by size:
3664 965 174
DraIII CAC_nnn'GTG
Cuts at: 0 1245 4803
Size: 1245 3558
DrdI GACnn_nn'nnGTC
Cuts at: 0 555 4803
Size: 555 4248
EaeI y'GGCC_r
Cuts at: 0 2591 3492 4803
Size: 2591 901 1311
Fragments arranged by size:
2591 1311 901
EarI CTCTTCn'nnn_
Cuts at: 0 500 695 707 1169 2695 3040 4031 4803
Size: 500 195 12 462 1526 345 991 772
Fragments arranged by size:
1526 991 772 500 462 345 195 12
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 3130 4803
Size: 3130 1673
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 320 835 932 2219 2780 3455 3529 3665
Size: 320 515 97 1287 561 675 74 136
Cuts at: 3665 3774 3923 4223 4290 4409 4508 4571 4803
Size: 109 149 300 67 119 99 63 232
Fragments arranged by size:
1287 675 561 515 320 300 232 149
136 119 109 99 97 74 67 63
EcoNI CCTnn'n_nnAGG
Cuts at: 0 3740 4742 4803
Size: 3740 1002 61
Fragments arranged by size:
3740 1002 61
EcoO109I rG'GnC_Cy
Cuts at: 0 676 3888 4564 4803
Size: 676 3212 676 239
Fragments arranged by size:
3212 676 676 239
EcoRI G'AATT_C
Cuts at: 0 2462 4803
Size: 2462 2341
EcoRV GAT'ATC
Cuts at: 0 618 2241 4803
Size: 618 1623 2562
Fragments arranged by size:
2562 1623 618
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1870 4112 4803
Size: 1870 2242 691
Fragments arranged by size:
2242 1870 691
HaeII r_GCGC'y
Cuts at: 0 26 453 4803
Size: 26 427 4350
Fragments arranged by size:
4350 427 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1521 1553 2042 2074 2428 2460 3430 3462
Size: 1521 32 489 32 354 32 970 32
Cuts at: 3462 3766 3798 3835 3867 3936 3968 4686 4718
Size: 304 32 37 32 69 32 718 32
Cuts at: 4718 4803
Size: 85
Fragments arranged by size:
1521 970 718 489 354 304 85 69
37 32 32 32 32 32 32 32
32
HincII GTy'rAC
Cuts at: 0 480 3843 3961 4803
Size: 480 3363 118 842
Fragments arranged by size:
3363 842 480 118
HpaI GTT'AAC
Cuts at: 0 480 4803
Size: 480 4323
KpnI G_GTAC'C
Cuts at: 0 3419 4803
Size: 3419 1384
MfeI C'AATT_G
Cuts at: 0 808 3831 4803
Size: 808 3023 972
Fragments arranged by size:
3023 972 808
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1333 2198 2393 4072 4276 4803
Size: 1333 865 195 1679 204 527
Fragments arranged by size:
1679 1333 865 527 204 195
MscI TGG'CCA
Cuts at: 0 2593 3494 4803
Size: 2593 901 1309
Fragments arranged by size:
2593 1309 901
MslI CAynn'nnrTG
Cuts at: 0 853 992 1457 1837 2212 2587 3396 4187
Size: 853 139 465 380 375 375 809 791
Cuts at: 4187 4803
Size: 616
Fragments arranged by size:
853 809 791 616 465 380 375 375
139
MspA1I CmG'CkG
Cuts at: 0 502 2601 3490 3556 4072 4378 4803
Size: 502 2099 889 66 516 306 425
Fragments arranged by size:
2099 889 516 502 425 306 66
NcoI C'CATG_G
Cuts at: 0 260 4803
Size: 260 4543
NdeI CA'TA_TG
Cuts at: 0 3411 4803
Size: 3411 1392
NsiI A_TGCA'T
Cuts at: 0 521 1067 1280 4628 4803
Size: 521 546 213 3348 175
Fragments arranged by size:
3348 546 521 213 175
NspI r_CATG'y
Cuts at: 0 1065 1942 3234 4803
Size: 1065 877 1292 1569
Fragments arranged by size:
1569 1292 1065 877
PasI CC'CwG_GG
Cuts at: 0 3970 4803
Size: 3970 833
PflMI CCAn_nnn'nTGG
Cuts at: 0 218 4339 4803
Size: 218 4121 464
Fragments arranged by size:
4121 464 218
PfoI T'CCnGG_A
Cuts at: 0 4524 4803
Size: 4524 279
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 737 769 3361 3393 3819 3851 4803
Size: 737 32 2592 32 426 32 952
Fragments arranged by size:
2592 952 737 426 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 676 4564 4803
Size: 676 3888 239
Fragments arranged by size:
3888 676 239
PshAI GACnn'nnGTC
Cuts at: 0 3198 4079 4803
Size: 3198 881 724
Fragments arranged by size:
3198 881 724
PsiI TTA'TAA
Cuts at: 0 2257 4427 4803
Size: 2257 2170 376
Fragments arranged by size:
2257 2170 376
PstI C_TGCA'G
Cuts at: 0 783 2201 2573 3858 4803
Size: 783 1418 372 1285 945
Fragments arranged by size:
1418 1285 945 783 372
PvuII CAG'CTG
Cuts at: 0 502 2601 3490 3556 4072 4378 4803
Size: 502 2099 889 66 516 306 425
Fragments arranged by size:
2099 889 516 502 425 306 66
SexAI A'CCwGG_T
Cuts at: 0 1686 4803
Size: 1686 3117
SfcI C'TryA_G
Cuts at: 0 779 2133 2197 2569 2882 3854 3862 4803
Size: 779 1354 64 372 313 972 8 941
Fragments arranged by size:
1354 972 941 779 372 313 64 8
SmlI C'TyrA_G
Cuts at: 0 1407 2534 3725 4046 4803
Size: 1407 1127 1191 321 757
Fragments arranged by size:
1407 1191 1127 757 321
SpeI A'CTAG_T
Cuts at: 0 2541 4803
Size: 2541 2262
SspI AAT'ATT
Cuts at: 0 110 1557 1764 2634 2994 4803
Size: 110 1447 207 870 360 1809
Fragments arranged by size:
1809 1447 870 360 207 110
StuI AGG'CCT
Cuts at: 0 2090 4803
Size: 2090 2713
StyI C'CwwG_G
Cuts at: 0 260 3131 4403 4803
Size: 260 2871 1272 400
Fragments arranged by size:
2871 1272 400 260
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2611 3962 4497 4803
Size: 2611 1351 535 306
Fragments arranged by size:
2611 1351 535 306
TatI w'GTAC_w
Cuts at: 0 1058 2648 4444 4466 4803
Size: 1058 1590 1796 22 337
Fragments arranged by size:
1796 1590 1058 337 22
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1229 1261 3741 3773 3859 3891 4803
Size: 1229 32 2480 32 86 32 912
Fragments arranged by size:
2480 1229 912 86 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 855 4803
Size: 855 3948
XbaI T'CTAG_A
Cuts at: 0 1494 2968 4252 4639 4803
Size: 1494 1474 1284 387 164
Fragments arranged by size:
1494 1474 1284 387 164
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2214 3915 4803
Size: 2214 1701 888
Fragments arranged by size:
2214 1701 888
XmnI GAAnn'nnTTC
Cuts at: 0 1325 3126 3159 4803
Size: 1325 1801 33 1644
Fragments arranged by size:
1801 1644 1325 33
Enzymes that do cut:
Acc65I AcuI AflII AflIII AgeI AhdI AlfI AloI
AlwNI ApoI AseI AvaI BamHI BanI BanII BbsI
BclI BfrBI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspHI
BspMI BsrBI BsrDI BsrFI BssSI BstAPI BstBI BstEII
BstXI BstYI Bsu36I BtgI BtsI ClaI DraI DraIII
DrdI EaeI EarI EciI Eco57MI EcoNI EcoO109I EcoRI
EcoRV FalI HaeII Hin4I HincII HpaI KpnI MfeI
MmeI MscI MslI MspA1I NcoI NdeI NsiI NspI
PasI PflMI PfoI PpiI PpuMI PshAI PsiI PstI
PvuII SexAI SfcI SmlI SpeI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AleI ApaI ApaLI
AscI AsiSI AvrII BaeI BbeI BbvCI BcgI BciVI
BlpI BmtI BsaI BsiEI BsiWI BspEI BsrGI BssHII
BstZ17I BtgZI CspCI EagI EcoICRI FseI FspI FspAI
HindIII KasI MluI NaeI NarI NgoMIV NheI NotI
NruI PacI PciI PmeI PmlI PspOMI PspXI PsrI
PvuI RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SphI
SrfI SwaI XhoI XmaI ZraI