Restriction map for
pF1KB3466
(Linear) (Six-base) MAPSORT of: KIBB3466.seq Check: 3322 from: 1 to: 1731
KIBB3466.orf
With 182 enzymes: SgfI *
April 24, 2009 13:01 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 595 600 1072 1208 1408 1585 1731
Size: 595 5 472 136 200 177 146
Fragments arranged by size:
595 472 200 177 146 136 5
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 112 144 1731
Size: 112 32 1587
Fragments arranged by size:
1587 112 32
AlwNI CAG_nnn'CTG
Cuts at: 0 300 1423 1731
Size: 300 1123 308
Fragments arranged by size:
1123 308 300
ApoI r'AATT_y
Cuts at: 0 760 1717 1731
Size: 760 957 14
Fragments arranged by size:
957 760 14
AvaI C'yCGr_G
Cuts at: 0 926 1220 1731
Size: 926 294 511
Fragments arranged by size:
926 511 294
BamHI G'GATC_C
Cuts at: 0 512 1731
Size: 512 1219
BanII G_rGCy'C
Cuts at: 0 1116 1731
Size: 1116 615
BbsI GAAGACnn'nnnn_
Cuts at: 0 795 1367 1378 1396 1618 1731
Size: 795 572 11 18 222 113
Fragments arranged by size:
795 572 222 113 18 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 34 1731
Size: 34 1697
BglI GCCn_nnn'nGGC
Cuts at: 0 1201 1731
Size: 1201 530
BglII A'GATC_T
Cuts at: 0 588 1731
Size: 588 1143
BlpI GC'TnA_GC
Cuts at: 0 1082 1382 1731
Size: 1082 300 349
Fragments arranged by size:
1082 349 300
Bme1580I G_kGCm'C
Cuts at: 0 1146 1731
Size: 1146 585
BmrI ACTGGGnnnn_n'
Cuts at: 0 1660 1731
Size: 1660 71
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1424 1731
Size: 1424 307
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 613 744 1312 1731
Size: 67 546 131 568 419
Fragments arranged by size:
568 546 419 131 67
Bpu10I CC'TnA_GC
Cuts at: 0 547 1731
Size: 547 1184
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 135 569 1144 1731
Size: 135 434 575 587
Fragments arranged by size:
587 575 434 135
BsaI GGTCTCn'nnnn_
Cuts at: 0 352 676 1731
Size: 352 324 1055
Fragments arranged by size:
1055 352 324
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 520 946 1048 1528 1731
Size: 520 426 102 480 203
Fragments arranged by size:
520 480 426 203 102
BseYI C'CCAG_C
Cuts at: 0 651 1286 1731
Size: 651 635 445
Fragments arranged by size:
651 635 445
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1573 1731
Size: 925 648 158
Fragments arranged by size:
925 648 158
BsmI GAATG_Cn'
Cuts at: 0 326 1731
Size: 326 1405
Bsp1286I G_dGCh'C
Cuts at: 0 1116 1146 1731
Size: 1116 30 585
Fragments arranged by size:
1116 585 30
BsrBI CCG'CTC
Cuts at: 0 510 1731
Size: 510 1221
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1423 1731
Size: 1423 308
BstYI r'GATC_y
Cuts at: 0 512 588 836 1682 1731
Size: 512 76 248 846 49
Fragments arranged by size:
846 512 248 76 49
Bsu36I CC'TnA_GG
Cuts at: 0 257 1731
Size: 257 1474
BtgI C'CryG_G
Cuts at: 0 32 537 1731
Size: 32 505 1194
Fragments arranged by size:
1194 505 32
BtsI GCAGTG_nn'
Cuts at: 0 1515 1731
Size: 1515 216
EaeI y'GGCC_r
Cuts at: 0 440 1166 1496 1586 1731
Size: 440 726 330 90 145
Fragments arranged by size:
726 440 330 145 90
EarI CTCTTCn'nnn_
Cuts at: 0 1184 1731
Size: 1184 547
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 595 600 613 744 1072 1208 1312
Size: 67 528 5 13 131 328 136 104
Cuts at: 1312 1408 1585 1731
Size: 96 177 146
Fragments arranged by size:
528 328 177 146 136 131 104 96
67 13 5
EcoO109I rG'GnC_Cy
Cuts at: 0 1569 1731
Size: 1569 162
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 732 855 1221 1731
Size: 732 123 366 510
Fragments arranged by size:
732 510 366 123
HaeII r_GCGC'y
Cuts at: 0 300 1731
Size: 300 1431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 250 282 1731
Size: 250 32 1449
Fragments arranged by size:
1449 250 32
MscI TGG'CCA
Cuts at: 0 1168 1498 1588 1731
Size: 1168 330 90 143
Fragments arranged by size:
1168 330 143 90
MslI CAynn'nnrTG
Cuts at: 0 901 1731
Size: 901 830
MspA1I CmG'CkG
Cuts at: 0 364 1248 1290 1731
Size: 364 884 42 441
Fragments arranged by size:
884 441 364 42
NcoI C'CATG_G
Cuts at: 0 32 1731
Size: 32 1699
NspI r_CATG'y
Cuts at: 0 546 876 1647 1731
Size: 546 330 771 84
Fragments arranged by size:
771 546 330 84
PasI CC'CwG_GG
Cuts at: 0 666 1731
Size: 666 1065
PfoI T'CCnGG_A
Cuts at: 0 470 563 1731
Size: 470 93 1168
Fragments arranged by size:
1168 470 93
PstI C_TGCA'G
Cuts at: 0 143 1214 1731
Size: 143 1071 517
Fragments arranged by size:
1071 517 143
PvuII CAG'CTG
Cuts at: 0 1248 1290 1731
Size: 1248 42 441
Fragments arranged by size:
1248 441 42
SexAI A'CCwGG_T
Cuts at: 0 92 1731
Size: 92 1639
SfcI C'TryA_G
Cuts at: 0 139 1210 1731
Size: 139 1071 521
Fragments arranged by size:
1071 521 139
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1201 1731
Size: 1201 530
SmaI CCC'GGG
Cuts at: 0 928 1222 1731
Size: 928 294 509
Fragments arranged by size:
928 509 294
SmlI C'TyrA_G
Cuts at: 0 114 584 1123 1731
Size: 114 470 539 608
Fragments arranged by size:
608 539 470 114
SphI G_CATG'C
Cuts at: 0 546 1731
Size: 546 1185
StuI AGG'CCT
Cuts at: 0 1204 1216 1731
Size: 1204 12 515
Fragments arranged by size:
1204 515 12
StyI C'CwwG_G
Cuts at: 0 32 1169 1196 1253 1499 1731
Size: 32 1137 27 57 246 232
Fragments arranged by size:
1137 246 232 57 32 27
TatI w'GTAC_w
Cuts at: 0 1402 1731
Size: 1402 329
Tth111I GACn'n_nGTC
Cuts at: 0 799 1731
Size: 799 932
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 397 1731
Size: 397 1334
XmaI C'CCGG_G
Cuts at: 0 926 1220 1731
Size: 926 294 511
Fragments arranged by size:
926 511 294
XmnI GAAnn'nnTTC
Cuts at: 0 278 613 1731
Size: 278 335 1118
Fragments arranged by size:
1118 335 278
Enzymes that do cut:
AcuI AloI AlwNI ApoI AvaI BamHI BanII BbsI
BcgI BglI BglII BlpI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaI BseRI BseYI BsgI BsmI Bsp1286I
BsrBI BstAPI BstYI Bsu36I BtgI BtsI EaeI EarI
Eco57MI EcoO109I FalI HaeII Hin4I MscI MslI MspA1I
NcoI NspI PasI PfoI PstI PvuII SexAI SfcI
SfiI SmaI SmlI SphI StuI StyI TatI Tth111I
XcmI XmaI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BanI BbeI BbvCI BciVI BclI
BfrBI BmgBI BmtI BsaAI BsaBI BsaHI BsaWI BsaXI
BsiEI BsiHKAI BsiWI BsmBI BspEI BspHI BspMI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DraIII DrdI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI MmeI NaeI
NarI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PsrI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SfoI SgfI SgrAI SnaBI
SpeI SrfI SspI SwaI TaqII TstI XbaI XhoI
ZraI