Restriction map for
pF1KB3471
(Linear) (Six-base) MAPSORT of: KIBB3471.seq Check: 3460 from: 1 to: 1602
KIBB3471.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 755 1602
Size: 755 847
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 1086 1141 1602
Size: 640 446 55 461
Fragments arranged by size:
640 461 446 55
AflIII A'CryG_T
Cuts at: 0 377 1602
Size: 377 1225
AhdI GACnn_n'nnGTC
Cuts at: 0 846 1602
Size: 846 756
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 574 1602
Size: 574 1028
AlwNI CAG_nnn'CTG
Cuts at: 0 96 684 852 1119 1602
Size: 96 588 168 267 483
Fragments arranged by size:
588 483 267 168 96
ApaI G_GGCC'C
Cuts at: 0 269 705 1602
Size: 269 436 897
Fragments arranged by size:
897 436 269
AvaI C'yCGr_G
Cuts at: 0 249 268 463 739 1059 1602
Size: 249 19 195 276 320 543
Fragments arranged by size:
543 320 276 249 195 19
BamHI G'GATC_C
Cuts at: 0 467 1602
Size: 467 1135
BanI G'GyrC_C
Cuts at: 0 324 356 1234 1246 1390 1575 1602
Size: 324 32 878 12 144 185 27
Fragments arranged by size:
878 324 185 144 32 27 12
BanII G_rGCy'C
Cuts at: 0 50 269 275 705 1602
Size: 50 219 6 430 897
Fragments arranged by size:
897 430 219 50 6
BbeI G_GCGC'C
Cuts at: 0 360 1238 1394 1602
Size: 360 878 156 208
Fragments arranged by size:
878 360 208 156
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 321 355 1602
Size: 321 34 1247
Fragments arranged by size:
1247 321 34
BclI T'GATC_A
Cuts at: 0 608 890 1602
Size: 608 282 712
Fragments arranged by size:
712 608 282
BglI GCCn_nnn'nGGC
Cuts at: 0 1266 1602
Size: 1266 336
BglII A'GATC_T
Cuts at: 0 1010 1040 1349 1448 1602
Size: 1010 30 309 99 154
Fragments arranged by size:
1010 309 154 99 30
Bme1580I G_kGCm'C
Cuts at: 0 269 417 705 903 1271 1602
Size: 269 148 288 198 368 331
Fragments arranged by size:
368 331 288 269 198 148
BmgBI CAC'GTC
Cuts at: 0 1308 1467 1602
Size: 1308 159 135
Fragments arranged by size:
1308 159 135
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 153 1602
Size: 153 1449
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 169 497 544 1555 1602
Size: 127 42 328 47 1011 47
Fragments arranged by size:
1011 328 127 47 47 42
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 925 1602
Size: 925 677
BsaBI GATnn'nnATC
Cuts at: 0 259 1602
Size: 259 1343
BsaHI Gr'CG_yC
Cuts at: 0 357 569 1235 1391 1602
Size: 357 212 666 156 211
Fragments arranged by size:
666 357 212 211 156
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 748 778 1027 1057 1602
Size: 748 30 249 30 545
Fragments arranged by size:
748 545 249 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 124 175 775 835 1213 1510 1602
Size: 124 51 600 60 378 297 92
Fragments arranged by size:
600 378 297 124 92 60 51
BseYI C'CCAG_C
Cuts at: 0 8 416 1602
Size: 8 408 1186
Fragments arranged by size:
1186 408 8
BsiHKAI G_wGCw'C
Cuts at: 0 50 116 185 1037 1602
Size: 50 66 69 852 565
Fragments arranged by size:
852 565 69 66 50
BsmBI CGTCTCn'nnnn_
Cuts at: 0 667 1602
Size: 667 935
Bsp1286I G_dGCh'C
Cuts at: 0 50 116 185 269 275 417 705 903
Size: 50 66 69 84 6 142 288 198
Cuts at: 903 1037 1271 1602
Size: 134 234 331
Fragments arranged by size:
331 288 234 198 142 134 84 69
66 50 6
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1568 1602
Size: 1568 34
BsrBI CCG'CTC
Cuts at: 0 783 1602
Size: 783 819
BsrFI r'CCGG_y
Cuts at: 0 1288 1602
Size: 1288 314
BssSI C'ACGA_G
Cuts at: 0 127 187 340 1543 1602
Size: 127 60 153 1203 59
Fragments arranged by size:
1203 153 127 60 59
BstAPI GCAn_nnn'nTGC
Cuts at: 0 96 687 1575 1602
Size: 96 591 888 27
Fragments arranged by size:
888 591 96 27
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1134 1602
Size: 1134 468
BstYI r'GATC_y
Cuts at: 0 467 638 1010 1040 1349 1448 1602
Size: 467 171 372 30 309 99 154
Fragments arranged by size:
467 372 309 171 154 99 30
Bsu36I CC'TnA_GG
Cuts at: 0 871 1602
Size: 871 731
BtgI C'CryG_G
Cuts at: 0 352 1602
Size: 352 1250
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 276 1152 1602
Size: 276 876 450
Fragments arranged by size:
876 450 276
BtsI GCAGTG_nn'
Cuts at: 0 850 1602
Size: 850 752
DraIII CAC_nnn'GTG
Cuts at: 0 1575 1602
Size: 1575 27
EaeI y'GGCC_r
Cuts at: 0 62 908 1157 1460 1508 1602
Size: 62 846 249 303 48 94
Fragments arranged by size:
846 303 249 94 62 48
EarI CTCTTCn'nnn_
Cuts at: 0 55 568 838 1602
Size: 55 513 270 764
Fragments arranged by size:
764 513 270 55
EcoICRI GAG'CTC
Cuts at: 0 48 1602
Size: 48 1554
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 127 169 497 544 640 1086 1141 1555
Size: 127 42 328 47 96 446 55 414
Cuts at: 1555 1602
Size: 47
Fragments arranged by size:
446 414 328 127 96 55 47 47
42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 835 1522 1602
Size: 835 687 80
Fragments arranged by size:
835 687 80
EcoO109I rG'GnC_Cy
Cuts at: 0 701 702 828 1602
Size: 701 1 126 774
Fragments arranged by size:
774 701 126 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 234 1602
Size: 234 1368
FspI TGC'GCA
Cuts at: 0 259 1602
Size: 259 1343
FspAI rTGC'GCAy
Cuts at: 0 259 1602
Size: 259 1343
HaeII r_GCGC'y
Cuts at: 0 360 1120 1238 1394 1602
Size: 360 760 118 156 208
Fragments arranged by size:
760 360 208 156 118
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 218 250 583 600 615 632 832 864
Size: 218 32 333 17 15 17 200 32
Cuts at: 864 1602
Size: 738
Fragments arranged by size:
738 333 218 200 32 32 17 17
15
HincII GTy'rAC
Cuts at: 0 1374 1602
Size: 1374 228
KasI G'GCGC_C
Cuts at: 0 356 1234 1390 1602
Size: 356 878 156 212
Fragments arranged by size:
878 356 212 156
MluI A'CGCG_T
Cuts at: 0 377 1602
Size: 377 1225
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 249 1602
Size: 249 1353
MscI TGG'CCA
Cuts at: 0 910 1159 1510 1602
Size: 910 249 351 92
Fragments arranged by size:
910 351 249 92
MspA1I CmG'CkG
Cuts at: 0 8 81 354 420 489 684 1602
Size: 8 73 273 66 69 195 918
Fragments arranged by size:
918 273 195 73 69 66 8
NaeI GCC'GGC
Cuts at: 0 1290 1602
Size: 1290 312
NarI GG'CG_CC
Cuts at: 0 357 1235 1391 1602
Size: 357 878 156 211
Fragments arranged by size:
878 357 211 156
NgoMIV G'CCGG_C
Cuts at: 0 1288 1602
Size: 1288 314
NspI r_CATG'y
Cuts at: 0 1155 1602
Size: 1155 447
PasI CC'CwG_GG
Cuts at: 0 717 1048 1602
Size: 717 331 554
Fragments arranged by size:
717 554 331
PflMI CCAn_nnn'nTGG
Cuts at: 0 60 579 1602
Size: 60 519 1023
Fragments arranged by size:
1023 519 60
PfoI T'CCnGG_A
Cuts at: 0 434 521 1602
Size: 434 87 1081
Fragments arranged by size:
1081 434 87
PpuMI rG'GwC_Cy
Cuts at: 0 828 1602
Size: 828 774
PspOMI G'GGCC_C
Cuts at: 0 265 701 1602
Size: 265 436 901
Fragments arranged by size:
901 436 265
PstI C_TGCA'G
Cuts at: 0 1091 1602
Size: 1091 511
PvuII CAG'CTG
Cuts at: 0 8 81 420 684 1602
Size: 8 73 339 264 918
Fragments arranged by size:
918 339 264 73 8
SacI G_AGCT'C
Cuts at: 0 50 1602
Size: 50 1552
SacII CC_GC'GG
Cuts at: 0 355 1602
Size: 355 1247
SapI GCTCTTCn'nnn_
Cuts at: 0 55 568 1602
Size: 55 513 1034
Fragments arranged by size:
1034 513 55
SexAI A'CCwGG_T
Cuts at: 0 1571 1602
Size: 1571 31
SfcI C'TryA_G
Cuts at: 0 1087 1602
Size: 1087 515
SfoI GGC'GCC
Cuts at: 0 358 1236 1392 1602
Size: 358 878 156 210
Fragments arranged by size:
878 358 210 156
SmaI CCC'GGG
Cuts at: 0 251 465 1602
Size: 251 214 1137
Fragments arranged by size:
1137 251 214
SmlI C'TyrA_G
Cuts at: 0 940 1602
Size: 940 662
StyI C'CwwG_G
Cuts at: 0 911 1602
Size: 911 691
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1305 1602
Size: 1305 297
TatI w'GTAC_w
Cuts at: 0 197 1602
Size: 197 1405
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1027 1059 1602
Size: 1027 32 543
Fragments arranged by size:
1027 543 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 318 432 1045 1407 1602
Size: 318 114 613 362 195
Fragments arranged by size:
613 362 318 195 114
XmaI C'CCGG_G
Cuts at: 0 249 463 1602
Size: 249 214 1139
Fragments arranged by size:
1139 249 214
Enzymes that do cut:
AccI AcuI AflIII AhdI AlfI AlwNI ApaI AvaI
BamHI BanI BanII BbeI BcgI BclI BglI BglII
Bme1580I BmgBI BplI BpmI BpuEI BsaBI BsaHI BsaXI
BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI
BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI
DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI
FspI FspAI HaeII Hin4I HincII KasI MluI MmeI
MscI MspA1I NaeI NarI NgoMIV NspI PasI PflMI
PfoI PpuMI PspOMI PstI PvuII SacI SacII SapI
SexAI SfcI SfoI SmaI SmlI StyI TaqII TatI
TstI XcmI XmaI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AleI
AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI
BbsI BbvCI BciVI BfrBI BlpI BmrI BmtI Bpu10I
BsaI BsaAI BsaWI BsgI BsiEI BsiWI BsmI BspEI
BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I ClaI
CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI
HindIII HpaI KpnI MfeI MslI NcoI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI
SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI
ZraI