(Linear) (Six-base) MAPSORT of: KIBB3471.seq Check: 3460 from: 1 to: 1602 KIBB3471.orf With 182 enzymes: SgfI * February 28, 2007 11:24 .. AccI GT'mk_AC Cuts at: 0 755 1602 Size: 755 847 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 640 1086 1141 1602 Size: 640 446 55 461 Fragments arranged by size: 640 461 446 55 AflIII A'CryG_T Cuts at: 0 377 1602 Size: 377 1225 AhdI GACnn_n'nnGTC Cuts at: 0 846 1602 Size: 846 756 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 574 1602 Size: 574 1028 AlwNI CAG_nnn'CTG Cuts at: 0 96 684 852 1119 1602 Size: 96 588 168 267 483 Fragments arranged by size: 588 483 267 168 96 ApaI G_GGCC'C Cuts at: 0 269 705 1602 Size: 269 436 897 Fragments arranged by size: 897 436 269 AvaI C'yCGr_G Cuts at: 0 249 268 463 739 1059 1602 Size: 249 19 195 276 320 543 Fragments arranged by size: 543 320 276 249 195 19 BamHI G'GATC_C Cuts at: 0 467 1602 Size: 467 1135 BanI G'GyrC_C Cuts at: 0 324 356 1234 1246 1390 1575 1602 Size: 324 32 878 12 144 185 27 Fragments arranged by size: 878 324 185 144 32 27 12 BanII G_rGCy'C Cuts at: 0 50 269 275 705 1602 Size: 50 219 6 430 897 Fragments arranged by size: 897 430 219 50 6 BbeI G_GCGC'C Cuts at: 0 360 1238 1394 1602 Size: 360 878 156 208 Fragments arranged by size: 878 360 208 156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 321 355 1602 Size: 321 34 1247 Fragments arranged by size: 1247 321 34 BclI T'GATC_A Cuts at: 0 608 890 1602 Size: 608 282 712 Fragments arranged by size: 712 608 282 BglI GCCn_nnn'nGGC Cuts at: 0 1266 1602 Size: 1266 336 BglII A'GATC_T Cuts at: 0 1010 1040 1349 1448 1602 Size: 1010 30 309 99 154 Fragments arranged by size: 1010 309 154 99 30 Bme1580I G_kGCm'C Cuts at: 0 269 417 705 903 1271 1602 Size: 269 148 288 198 368 331 Fragments arranged by size: 368 331 288 269 198 148 BmgBI CAC'GTC Cuts at: 0 1308 1467 1602 Size: 1308 159 135 Fragments arranged by size: 1308 159 135 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 0 153 1602 Size: 153 1449 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 169 497 544 1555 1602 Size: 127 42 328 47 1011 47 Fragments arranged by size: 1011 328 127 47 47 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 925 1602 Size: 925 677 BsaBI GATnn'nnATC Cuts at: 0 259 1602 Size: 259 1343 BsaHI Gr'CG_yC Cuts at: 0 357 569 1235 1391 1602 Size: 357 212 666 156 211 Fragments arranged by size: 666 357 212 211 156 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 748 778 1027 1057 1602 Size: 748 30 249 30 545 Fragments arranged by size: 748 545 249 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 124 175 775 835 1213 1510 1602 Size: 124 51 600 60 378 297 92 Fragments arranged by size: 600 378 297 124 92 60 51 BseYI C'CCAG_C Cuts at: 0 8 416 1602 Size: 8 408 1186 Fragments arranged by size: 1186 408 8 BsiHKAI G_wGCw'C Cuts at: 0 50 116 185 1037 1602 Size: 50 66 69 852 565 Fragments arranged by size: 852 565 69 66 50 BsmBI CGTCTCn'nnnn_ Cuts at: 0 667 1602 Size: 667 935 Bsp1286I G_dGCh'C Cuts at: 0 50 116 185 269 275 417 705 903 Size: 50 66 69 84 6 142 288 198 Cuts at: 903 1037 1271 1602 Size: 134 234 331 Fragments arranged by size: 331 288 234 198 142 134 84 69 66 50 6 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1568 1602 Size: 1568 34 BsrBI CCG'CTC Cuts at: 0 783 1602 Size: 783 819 BsrFI r'CCGG_y Cuts at: 0 1288 1602 Size: 1288 314 BssSI C'ACGA_G Cuts at: 0 127 187 340 1543 1602 Size: 127 60 153 1203 59 Fragments arranged by size: 1203 153 127 60 59 BstAPI GCAn_nnn'nTGC Cuts at: 0 96 687 1575 1602 Size: 96 591 888 27 Fragments arranged by size: 888 591 96 27 BstXI CCAn_nnnn'nTGG Cuts at: 0 1134 1602 Size: 1134 468 BstYI r'GATC_y Cuts at: 0 467 638 1010 1040 1349 1448 1602 Size: 467 171 372 30 309 99 154 Fragments arranged by size: 467 372 309 171 154 99 30 Bsu36I CC'TnA_GG Cuts at: 0 871 1602 Size: 871 731 BtgI C'CryG_G Cuts at: 0 352 1602 Size: 352 1250 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 276 1152 1602 Size: 276 876 450 Fragments arranged by size: 876 450 276 BtsI GCAGTG_nn' Cuts at: 0 850 1602 Size: 850 752 DraIII CAC_nnn'GTG Cuts at: 0 1575 1602 Size: 1575 27 EaeI y'GGCC_r Cuts at: 0 62 908 1157 1460 1508 1602 Size: 62 846 249 303 48 94 Fragments arranged by size: 846 303 249 94 62 48 EarI CTCTTCn'nnn_ Cuts at: 0 55 568 838 1602 Size: 55 513 270 764 Fragments arranged by size: 764 513 270 55 EcoICRI GAG'CTC Cuts at: 0 48 1602 Size: 48 1554 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 127 169 497 544 640 1086 1141 1555 Size: 127 42 328 47 96 446 55 414 Cuts at: 1555 1602 Size: 47 Fragments arranged by size: 446 414 328 127 96 55 47 47 42 EcoNI CCTnn'n_nnAGG Cuts at: 0 835 1522 1602 Size: 835 687 80 Fragments arranged by size: 835 687 80 EcoO109I rG'GnC_Cy Cuts at: 0 701 702 828 1602 Size: 701 1 126 774 Fragments arranged by size: 774 701 126 1 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 234 1602 Size: 234 1368 FspI TGC'GCA Cuts at: 0 259 1602 Size: 259 1343 FspAI rTGC'GCAy Cuts at: 0 259 1602 Size: 259 1343 HaeII r_GCGC'y Cuts at: 0 360 1120 1238 1394 1602 Size: 360 760 118 156 208 Fragments arranged by size: 760 360 208 156 118 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 218 250 583 600 615 632 832 864 Size: 218 32 333 17 15 17 200 32 Cuts at: 864 1602 Size: 738 Fragments arranged by size: 738 333 218 200 32 32 17 17 15 HincII GTy'rAC Cuts at: 0 1374 1602 Size: 1374 228 KasI G'GCGC_C Cuts at: 0 356 1234 1390 1602 Size: 356 878 156 212 Fragments arranged by size: 878 356 212 156 MluI A'CGCG_T Cuts at: 0 377 1602 Size: 377 1225 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 249 1602 Size: 249 1353 MscI TGG'CCA Cuts at: 0 910 1159 1510 1602 Size: 910 249 351 92 Fragments arranged by size: 910 351 249 92 MspA1I CmG'CkG Cuts at: 0 8 81 354 420 489 684 1602 Size: 8 73 273 66 69 195 918 Fragments arranged by size: 918 273 195 73 69 66 8 NaeI GCC'GGC Cuts at: 0 1290 1602 Size: 1290 312 NarI GG'CG_CC Cuts at: 0 357 1235 1391 1602 Size: 357 878 156 211 Fragments arranged by size: 878 357 211 156 NgoMIV G'CCGG_C Cuts at: 0 1288 1602 Size: 1288 314 NspI r_CATG'y Cuts at: 0 1155 1602 Size: 1155 447 PasI CC'CwG_GG Cuts at: 0 717 1048 1602 Size: 717 331 554 Fragments arranged by size: 717 554 331 PflMI CCAn_nnn'nTGG Cuts at: 0 60 579 1602 Size: 60 519 1023 Fragments arranged by size: 1023 519 60 PfoI T'CCnGG_A Cuts at: 0 434 521 1602 Size: 434 87 1081 Fragments arranged by size: 1081 434 87 PpuMI rG'GwC_Cy Cuts at: 0 828 1602 Size: 828 774 PspOMI G'GGCC_C Cuts at: 0 265 701 1602 Size: 265 436 901 Fragments arranged by size: 901 436 265 PstI C_TGCA'G Cuts at: 0 1091 1602 Size: 1091 511 PvuII CAG'CTG Cuts at: 0 8 81 420 684 1602 Size: 8 73 339 264 918 Fragments arranged by size: 918 339 264 73 8 SacI G_AGCT'C Cuts at: 0 50 1602 Size: 50 1552 SacII CC_GC'GG Cuts at: 0 355 1602 Size: 355 1247 SapI GCTCTTCn'nnn_ Cuts at: 0 55 568 1602 Size: 55 513 1034 Fragments arranged by size: 1034 513 55 SexAI A'CCwGG_T Cuts at: 0 1571 1602 Size: 1571 31 SfcI C'TryA_G Cuts at: 0 1087 1602 Size: 1087 515 SfoI GGC'GCC Cuts at: 0 358 1236 1392 1602 Size: 358 878 156 210 Fragments arranged by size: 878 358 210 156 SmaI CCC'GGG Cuts at: 0 251 465 1602 Size: 251 214 1137 Fragments arranged by size: 1137 251 214 SmlI C'TyrA_G Cuts at: 0 940 1602 Size: 940 662 StyI C'CwwG_G Cuts at: 0 911 1602 Size: 911 691 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1305 1602 Size: 1305 297 TatI w'GTAC_w Cuts at: 0 197 1602 Size: 197 1405 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 1027 1059 1602 Size: 1027 32 543 Fragments arranged by size: 1027 543 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 318 432 1045 1407 1602 Size: 318 114 613 362 195 Fragments arranged by size: 613 362 318 195 114 XmaI C'CCGG_G Cuts at: 0 249 463 1602 Size: 249 214 1139 Fragments arranged by size: 1139 249 214 Enzymes that do cut: AccI AcuI AflIII AhdI AlfI AlwNI ApaI AvaI BamHI BanI BanII BbeI BcgI BclI BglI BglII Bme1580I BmgBI BplI BpmI BpuEI BsaBI BsaHI BsaXI BseRI BseYI BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI FspAI HaeII Hin4I HincII KasI MluI MmeI MscI MspA1I NaeI NarI NgoMIV NspI PasI PflMI PfoI PpuMI PspOMI PstI PvuII SacI SacII SapI SexAI SfcI SfoI SmaI SmlI StyI TaqII TatI TstI XcmI XmaI Enzymes that do not cut: AarI AatII Acc65I AclI AfeI AflII AgeI AleI AloI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BbsI BbvCI BciVI BfrBI BlpI BmrI BmtI Bpu10I BsaI BsaAI BsaWI BsgI BsiEI BsiWI BsmI BspEI BspHI BsrDI BsrGI BssHII BstBI BstEII BstZ17I ClaI CspCI DraI DrdI EagI EciI EcoRI EcoRV FseI HindIII HpaI KpnI MfeI MslI NcoI NdeI NheI NotI NruI NsiI PacI PciI PmeI PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI SbfI ScaI SfiI SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmnI ZraI