Restriction map for pF1KB3471
 (Linear) (Six-base) MAPSORT of: KIBB3471.seq  Check: 3460  from: 1  to: 1602



KIBB3471.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AccI GT'mk_AC

Cuts at:      0     755    1602 
   Size:        755     847
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     640    1086    1141    1602 
   Size:        640     446      55     461

  Fragments arranged by size: 

                 640     461     446      55
 
AflIII A'CryG_T

Cuts at:      0     377    1602 
   Size:        377    1225
 
AhdI GACnn_n'nnGTC

Cuts at:      0     846    1602 
   Size:        846     756
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     574    1602 
   Size:        574    1028
 
AlwNI CAG_nnn'CTG

Cuts at:      0      96     684     852    1119    1602 
   Size:         96     588     168     267     483

  Fragments arranged by size: 

                 588     483     267     168      96
 
ApaI G_GGCC'C

Cuts at:      0     269     705    1602 
   Size:        269     436     897

  Fragments arranged by size: 

                 897     436     269
 
AvaI C'yCGr_G

Cuts at:      0     249     268     463     739    1059    1602 
   Size:        249      19     195     276     320     543

  Fragments arranged by size: 

                 543     320     276     249     195      19
 
BamHI G'GATC_C

Cuts at:      0     467    1602 
   Size:        467    1135
 
BanI G'GyrC_C

Cuts at:      0     324     356    1234    1246    1390    1575    1602 
   Size:        324      32     878      12     144     185      27

  Fragments arranged by size: 

                 878     324     185     144      32      27      12
 
BanII G_rGCy'C

Cuts at:      0      50     269     275     705    1602 
   Size:         50     219       6     430     897

  Fragments arranged by size: 

                 897     430     219      50       6
 
BbeI G_GCGC'C

Cuts at:      0     360    1238    1394    1602 
   Size:        360     878     156     208

  Fragments arranged by size: 

                 878     360     208     156
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     321     355    1602 
   Size:        321      34    1247

  Fragments arranged by size: 

                1247     321      34
 
BclI T'GATC_A

Cuts at:      0     608     890    1602 
   Size:        608     282     712

  Fragments arranged by size: 

                 712     608     282
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1266    1602 
   Size:       1266     336
 
BglII A'GATC_T

Cuts at:      0    1010    1040    1349    1448    1602 
   Size:       1010      30     309      99     154

  Fragments arranged by size: 

                1010     309     154      99      30
 
Bme1580I G_kGCm'C

Cuts at:      0     269     417     705     903    1271    1602 
   Size:        269     148     288     198     368     331

  Fragments arranged by size: 

                 368     331     288     269     198     148
 
BmgBI CAC'GTC

Cuts at:      0    1308    1467    1602 
   Size:       1308     159     135

  Fragments arranged by size: 

                1308     159     135
 
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     153    1602 
   Size:        153    1449
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     169     497     544    1555    1602 
   Size:        127      42     328      47    1011      47

  Fragments arranged by size: 

                1011     328     127      47      47      42
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     925    1602 
   Size:        925     677
 
BsaBI GATnn'nnATC

Cuts at:      0     259    1602 
   Size:        259    1343
 
BsaHI Gr'CG_yC

Cuts at:      0     357     569    1235    1391    1602 
   Size:        357     212     666     156     211

  Fragments arranged by size: 

                 666     357     212     211     156
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     748     778    1027    1057    1602 
   Size:        748      30     249      30     545

  Fragments arranged by size: 

                 748     545     249      30      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     124     175     775     835    1213    1510    1602 
   Size:        124      51     600      60     378     297      92

  Fragments arranged by size: 

                 600     378     297     124      92      60      51
 
BseYI C'CCAG_C

Cuts at:      0       8     416    1602 
   Size:          8     408    1186

  Fragments arranged by size: 

                1186     408       8
 
BsiHKAI G_wGCw'C

Cuts at:      0      50     116     185    1037    1602 
   Size:         50      66      69     852     565

  Fragments arranged by size: 

                 852     565      69      66      50
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     667    1602 
   Size:        667     935
 
Bsp1286I G_dGCh'C

Cuts at:      0      50     116     185     269     275     417     705     903 
   Size:         50      66      69      84       6     142     288     198

Cuts at:    903    1037    1271    1602 
   Size:        134     234     331

  Fragments arranged by size: 

                 331     288     234     198     142     134      84      69
                  66      50       6
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1568    1602 
   Size:       1568      34
 
BsrBI CCG'CTC

Cuts at:      0     783    1602 
   Size:        783     819
 
BsrFI r'CCGG_y

Cuts at:      0    1288    1602 
   Size:       1288     314
 
BssSI C'ACGA_G

Cuts at:      0     127     187     340    1543    1602 
   Size:        127      60     153    1203      59

  Fragments arranged by size: 

                1203     153     127      60      59
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0      96     687    1575    1602 
   Size:         96     591     888      27

  Fragments arranged by size: 

                 888     591      96      27
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0    1134    1602 
   Size:       1134     468
 
BstYI r'GATC_y

Cuts at:      0     467     638    1010    1040    1349    1448    1602 
   Size:        467     171     372      30     309      99     154

  Fragments arranged by size: 

                 467     372     309     171     154      99      30
 
Bsu36I CC'TnA_GG

Cuts at:      0     871    1602 
   Size:        871     731
 
BtgI C'CryG_G

Cuts at:      0     352    1602 
   Size:        352    1250
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     276    1152    1602 
   Size:        276     876     450

  Fragments arranged by size: 

                 876     450     276
 
BtsI GCAGTG_nn'

Cuts at:      0     850    1602 
   Size:        850     752
 
DraIII CAC_nnn'GTG

Cuts at:      0    1575    1602 
   Size:       1575      27
 
EaeI y'GGCC_r

Cuts at:      0      62     908    1157    1460    1508    1602 
   Size:         62     846     249     303      48      94

  Fragments arranged by size: 

                 846     303     249      94      62      48
 
EarI CTCTTCn'nnn_

Cuts at:      0      55     568     838    1602 
   Size:         55     513     270     764

  Fragments arranged by size: 

                 764     513     270      55
 
EcoICRI GAG'CTC

Cuts at:      0      48    1602 
   Size:         48    1554
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     127     169     497     544     640    1086    1141    1555 
   Size:        127      42     328      47      96     446      55     414

Cuts at:   1555    1602 
   Size:         47

  Fragments arranged by size: 

                 446     414     328     127      96      55      47      47
                  42
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0     835    1522    1602 
   Size:        835     687      80

  Fragments arranged by size: 

                 835     687      80
 
EcoO109I rG'GnC_Cy

Cuts at:      0     701     702     828    1602 
   Size:        701       1     126     774

  Fragments arranged by size: 

                 774     701     126       1
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     234    1602 
   Size:        234    1368
 
FspI TGC'GCA

Cuts at:      0     259    1602 
   Size:        259    1343
 
FspAI rTGC'GCAy

Cuts at:      0     259    1602 
   Size:        259    1343
 
HaeII r_GCGC'y

Cuts at:      0     360    1120    1238    1394    1602 
   Size:        360     760     118     156     208

  Fragments arranged by size: 

                 760     360     208     156     118
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     218     250     583     600     615     632     832     864 
   Size:        218      32     333      17      15      17     200      32

Cuts at:    864    1602 
   Size:        738

  Fragments arranged by size: 

                 738     333     218     200      32      32      17      17
                  15
 
HincII GTy'rAC

Cuts at:      0    1374    1602 
   Size:       1374     228
 
KasI G'GCGC_C

Cuts at:      0     356    1234    1390    1602 
   Size:        356     878     156     212

  Fragments arranged by size: 

                 878     356     212     156
 
MluI A'CGCG_T

Cuts at:      0     377    1602 
   Size:        377    1225
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     249    1602 
   Size:        249    1353
 
MscI TGG'CCA

Cuts at:      0     910    1159    1510    1602 
   Size:        910     249     351      92

  Fragments arranged by size: 

                 910     351     249      92
 
MspA1I CmG'CkG

Cuts at:      0       8      81     354     420     489     684    1602 
   Size:          8      73     273      66      69     195     918

  Fragments arranged by size: 

                 918     273     195      73      69      66       8
 
NaeI GCC'GGC

Cuts at:      0    1290    1602 
   Size:       1290     312
 
NarI GG'CG_CC

Cuts at:      0     357    1235    1391    1602 
   Size:        357     878     156     211

  Fragments arranged by size: 

                 878     357     211     156
 
NgoMIV G'CCGG_C

Cuts at:      0    1288    1602 
   Size:       1288     314
 
NspI r_CATG'y

Cuts at:      0    1155    1602 
   Size:       1155     447
 
PasI CC'CwG_GG

Cuts at:      0     717    1048    1602 
   Size:        717     331     554

  Fragments arranged by size: 

                 717     554     331
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      60     579    1602 
   Size:         60     519    1023

  Fragments arranged by size: 

                1023     519      60
 
PfoI T'CCnGG_A

Cuts at:      0     434     521    1602 
   Size:        434      87    1081

  Fragments arranged by size: 

                1081     434      87
 
PpuMI rG'GwC_Cy

Cuts at:      0     828    1602 
   Size:        828     774
 
PspOMI G'GGCC_C

Cuts at:      0     265     701    1602 
   Size:        265     436     901

  Fragments arranged by size: 

                 901     436     265
 
PstI C_TGCA'G

Cuts at:      0    1091    1602 
   Size:       1091     511
 
PvuII CAG'CTG

Cuts at:      0       8      81     420     684    1602 
   Size:          8      73     339     264     918

  Fragments arranged by size: 

                 918     339     264      73       8
 
SacI G_AGCT'C

Cuts at:      0      50    1602 
   Size:         50    1552
 
SacII CC_GC'GG

Cuts at:      0     355    1602 
   Size:        355    1247
 
SapI GCTCTTCn'nnn_

Cuts at:      0      55     568    1602 
   Size:         55     513    1034

  Fragments arranged by size: 

                1034     513      55
 
SexAI A'CCwGG_T

Cuts at:      0    1571    1602 
   Size:       1571      31
 
SfcI C'TryA_G

Cuts at:      0    1087    1602 
   Size:       1087     515
 
SfoI GGC'GCC

Cuts at:      0     358    1236    1392    1602 
   Size:        358     878     156     210

  Fragments arranged by size: 

                 878     358     210     156
 
SmaI CCC'GGG

Cuts at:      0     251     465    1602 
   Size:        251     214    1137

  Fragments arranged by size: 

                1137     251     214
 
SmlI C'TyrA_G

Cuts at:      0     940    1602 
   Size:        940     662
 
StyI C'CwwG_G

Cuts at:      0     911    1602 
   Size:        911     691
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1305    1602 
   Size:       1305     297
 
TatI w'GTAC_w

Cuts at:      0     197    1602 
   Size:        197    1405
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0    1027    1059    1602 
   Size:       1027      32     543

  Fragments arranged by size: 

                1027     543      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     318     432    1045    1407    1602 
   Size:        318     114     613     362     195

  Fragments arranged by size: 

                 613     362     318     195     114
 
XmaI C'CCGG_G

Cuts at:      0     249     463    1602 
   Size:        249     214    1139

  Fragments arranged by size: 

                1139     249     214
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AhdI     AlfI    AlwNI     ApaI     AvaI
    BamHI     BanI    BanII     BbeI     BcgI     BclI     BglI    BglII
 Bme1580I    BmgBI     BplI     BpmI    BpuEI    BsaBI    BsaHI    BsaXI
    BseRI    BseYI  BsiHKAI    BsmBI Bsp1286I    BspMI    BsrBI    BsrFI
    BssSI   BstAPI    BstXI    BstYI   Bsu36I     BtgI    BtgZI     BtsI
   DraIII     EaeI     EarI  EcoICRI  Eco57MI    EcoNI EcoO109I     FalI
     FspI    FspAI    HaeII    Hin4I   HincII     KasI     MluI     MmeI
     MscI   MspA1I     NaeI     NarI   NgoMIV     NspI     PasI    PflMI
     PfoI    PpuMI   PspOMI     PstI    PvuII     SacI    SacII     SapI
    SexAI     SfcI     SfoI     SmaI     SmlI     StyI    TaqII     TatI
     TstI     XcmI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AleI
     AloI    ApaLI     ApoI     AscI     AseI    AsiSI    AvrII     BaeI
     BbsI    BbvCI    BciVI    BfrBI     BlpI     BmrI     BmtI   Bpu10I
     BsaI    BsaAI    BsaWI     BsgI    BsiEI    BsiWI     BsmI    BspEI
    BspHI    BsrDI    BsrGI   BssHII    BstBI   BstEII  BstZ17I     ClaI
    CspCI     DraI     DrdI     EagI     EciI    EcoRI    EcoRV     FseI
  HindIII     HpaI     KpnI     MfeI     MslI     NcoI     NdeI     NheI
     NotI     NruI     NsiI     PacI     PciI     PmeI     PmlI     PpiI
    PshAI     PsiI    PspXI     PsrI     PvuI    RsrII     SalI    SanDI
     SbfI     ScaI     SfiI     SgfI    SgrAI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     SwaI  Tth111I     XbaI     XhoI     XmnI
     ZraI
Inquiries or Suggestions ?
Send a message to flexiclone AT kazusagt.com