Restriction map for
pF1KB3473
(Linear) (Six-base) MAPSORT of: KIBB3473.seq Check: 5572 from: 1 to: 1491
KIBB3473.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 598 1491
Size: 598 893
AccI GT'mk_AC
Cuts at: 0 205 1491
Size: 205 1286
AclI AA'CG_TT
Cuts at: 0 511 1491
Size: 511 980
AfeI AGC'GCT
Cuts at: 0 1002 1491
Size: 1002 489
AflIII A'CryG_T
Cuts at: 0 1478 1491
Size: 1478 13
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 760 1491
Size: 760 731
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 861 893 1491
Size: 861 32 598
Fragments arranged by size:
861 598 32
ApoI r'AATT_y
Cuts at: 0 702 1491
Size: 702 789
AseI AT'TA_AT
Cuts at: 0 82 1151 1491
Size: 82 1069 340
Fragments arranged by size:
1069 340 82
AvaI C'yCGr_G
Cuts at: 0 1199 1491
Size: 1199 292
AvrII C'CTAG_G
Cuts at: 0 1083 1491
Size: 1083 408
BamHI G'GATC_C
Cuts at: 0 1383 1491
Size: 1383 108
BanII G_rGCy'C
Cuts at: 0 295 579 1491
Size: 295 284 912
Fragments arranged by size:
912 295 284
BbvCI CC'TCA_GC
Cuts at: 0 1099 1491
Size: 1099 392
BglII A'GATC_T
Cuts at: 0 807 1491
Size: 807 684
BmrI ACTGGGnnnn_n'
Cuts at: 0 901 1491
Size: 901 590
BmtI G_CTAG'C
Cuts at: 0 176 1491
Size: 176 1315
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1136 1491
Size: 593 543 355
Fragments arranged by size:
593 543 355
Bpu10I CC'TnA_GC
Cuts at: 0 732 1099 1491
Size: 732 367 392
Fragments arranged by size:
732 392 367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 184 672 866 1491
Size: 184 488 194 625
Fragments arranged by size:
625 488 194 184
BsaI GGTCTCn'nnnn_
Cuts at: 0 579 1491
Size: 579 912
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 741 771 1491
Size: 741 30 720
Fragments arranged by size:
741 720 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 569 1187 1491
Size: 569 618 304
Fragments arranged by size:
618 569 304
BseYI C'CCAG_C
Cuts at: 0 1050 1280 1301 1491
Size: 1050 230 21 190
Fragments arranged by size:
1050 230 190 21
BsiHKAI G_wGCw'C
Cuts at: 0 113 320 579 625 1491
Size: 113 207 259 46 866
Fragments arranged by size:
866 259 207 113 46
BsmI GAATG_Cn'
Cuts at: 0 1046 1491
Size: 1046 445
Bsp1286I G_dGCh'C
Cuts at: 0 113 295 320 579 625 1491
Size: 113 182 25 259 46 866
Fragments arranged by size:
866 259 182 113 46 25
BspHI T'CATG_A
Cuts at: 0 225 1491
Size: 225 1266
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 598 1491
Size: 598 893
BsrDI GCAATG_nn'
Cuts at: 0 609 968 1491
Size: 609 359 523
Fragments arranged by size:
609 523 359
BstEII G'GTnAC_C
Cuts at: 0 1485 1491
Size: 1485 6
BstYI r'GATC_y
Cuts at: 0 807 1383 1491
Size: 807 576 108
Fragments arranged by size:
807 576 108
BstZ17I GTA'TAC
Cuts at: 0 206 1491
Size: 206 1285
BtgI C'CryG_G
Cuts at: 0 1387 1491
Size: 1387 104
BtsI GCAGTG_nn'
Cuts at: 0 1377 1491
Size: 1377 114
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 591 626 1491
Size: 591 35 865
Fragments arranged by size:
865 591 35
DraIII CAC_nnn'GTG
Cuts at: 0 772 1491
Size: 772 719
EaeI y'GGCC_r
Cuts at: 0 1419 1491
Size: 1419 72
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 21 1491
Size: 21 1470
EcoICRI GAG'CTC
Cuts at: 0 577 1491
Size: 577 914
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 593 1136 1491
Size: 593 543 355
Fragments arranged by size:
593 543 355
EcoNI CCTnn'n_nnAGG
Cuts at: 0 767 941 1491
Size: 767 174 550
Fragments arranged by size:
767 550 174
EcoO109I rG'GnC_Cy
Cuts at: 0 385 1491
Size: 385 1106
HaeII r_GCGC'y
Cuts at: 0 1004 1326 1491
Size: 1004 322 165
Fragments arranged by size:
1004 322 165
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 806 838 1179 1211 1491
Size: 806 32 341 32 280
Fragments arranged by size:
806 341 280 32 32
HincII GTy'rAC
Cuts at: 0 515 1491
Size: 515 976
HindIII A'AGCT_T
Cuts at: 0 930 1491
Size: 930 561
HpaI GTT'AAC
Cuts at: 0 515 1491
Size: 515 976
MfeI C'AATT_G
Cuts at: 0 1409 1491
Size: 1409 82
MluI A'CGCG_T
Cuts at: 0 1478 1491
Size: 1478 13
MslI CAynn'nnrTG
Cuts at: 0 181 560 1491
Size: 181 379 931
Fragments arranged by size:
931 379 181
NheI G'CTAG_C
Cuts at: 0 172 1491
Size: 172 1319
NspI r_CATG'y
Cuts at: 0 531 1491
Size: 531 960
PasI CC'CwG_GG
Cuts at: 0 469 1491
Size: 469 1022
PflMI CCAn_nnn'nTGG
Cuts at: 0 598 772 775 1491
Size: 598 174 3 716
Fragments arranged by size:
716 598 174 3
PfoI T'CCnGG_A
Cuts at: 0 570 1491
Size: 570 921
PshAI GACnn'nnGTC
Cuts at: 0 1059 1491
Size: 1059 432
SacI G_AGCT'C
Cuts at: 0 579 1491
Size: 579 912
ScaI AGT'ACT
Cuts at: 0 597 1491
Size: 597 894
SexAI A'CCwGG_T
Cuts at: 0 768 1491
Size: 768 723
SfcI C'TryA_G
Cuts at: 0 626 1491
Size: 626 865
SmlI C'TyrA_G
Cuts at: 0 199 687 881 1491
Size: 199 488 194 610
Fragments arranged by size:
610 488 199 194
SphI G_CATG'C
Cuts at: 0 531 1491
Size: 531 960
StuI AGG'CCT
Cuts at: 0 275 1491
Size: 275 1216
StyI C'CwwG_G
Cuts at: 0 1083 1491
Size: 1083 408
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 877 1473 1491
Size: 877 596 18
Fragments arranged by size:
877 596 18
TatI w'GTAC_w
Cuts at: 0 595 953 1491
Size: 595 358 538
Fragments arranged by size:
595 538 358
Enzymes that do cut:
AarI AccI AclI AfeI AflIII AlfI AloI ApoI
AseI AvaI AvrII BamHI BanII BbvCI BglII BmrI
BmtI BpmI Bpu10I BpuEI BsaI BsaXI BseRI BseYI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrDI BstEII BstYI
BstZ17I BtgI BtsI CspCI DraIII EaeI EciI EcoICRI
Eco57MI EcoNI EcoO109I HaeII Hin4I HincII HindIII HpaI
MfeI MluI MslI NheI NspI PasI PflMI PfoI
PshAI SacI ScaI SexAI SfcI SmlI SphI StuI
StyI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AcuI AflII AgeI AhdI AleI AlwNI
ApaI ApaLI AscI AsiSI BaeI BanI BbeI BbsI
BcgI BciVI BclI BfrBI BglI BlpI Bme1580I BmgBI
BplI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI BsiWI
BsmBI BspEI BsrBI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstXI Bsu36I BtgZI ClaI DraI DrdI EagI
EarI EcoRI EcoRV FalI FseI FspI FspAI KasI
KpnI MmeI MscI MspA1I NaeI NarI NcoI NdeI
NgoMIV NotI NruI NsiI PacI PciI PmeI PmlI
PpiI PpuMI PsiI PspOMI PspXI PsrI PstI PvuI
PvuII RsrII SacII SalI SanDI SapI SbfI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SrfI SspI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI