Restriction map for
pF1KB3480
(Linear) (Six-base) MAPSORT of: KIBB3480.seq Check: 4943 from: 1 to: 948
KIBB3480.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 0 789 948
Size: 789 159
AflIII A'CryG_T
Cuts at: 0 302 948
Size: 302 646
AleI CACnn'nnGTG
Cuts at: 0 542 948
Size: 542 406
ApoI r'AATT_y
Cuts at: 0 487 948
Size: 487 461
BamHI G'GATC_C
Cuts at: 0 501 948
Size: 501 447
BanI G'GyrC_C
Cuts at: 0 422 879 948
Size: 422 457 69
Fragments arranged by size:
457 422 69
BanII G_rGCy'C
Cuts at: 0 137 192 948
Size: 137 55 756
Fragments arranged by size:
756 137 55
BbvCI CC'TCA_GC
Cuts at: 0 328 418 948
Size: 328 90 530
Fragments arranged by size:
530 328 90
BglI GCCn_nnn'nGGC
Cuts at: 0 314 948
Size: 314 634
Bme1580I G_kGCm'C
Cuts at: 0 620 882 948
Size: 620 262 66
Fragments arranged by size:
620 262 66
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 225 948
Size: 225 723
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 358 409 948
Size: 139 219 51 539
Fragments arranged by size:
539 219 139 51
Bpu10I CC'TnA_GC
Cuts at: 0 328 418 948
Size: 328 90 530
Fragments arranged by size:
530 328 90
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 151 948
Size: 151 797
BsaI GGTCTCn'nnnn_
Cuts at: 0 4 948
Size: 4 944
BsaAI yAC'GTr
Cuts at: 0 305 948
Size: 305 643
BsaBI GATnn'nnATC
Cuts at: 0 799 948
Size: 799 149
BsaHI Gr'CG_yC
Cuts at: 0 786 948
Size: 786 162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 289 319 903 933 948
Size: 289 30 584 30 15
Fragments arranged by size:
584 289 30 30 15
BseYI C'CCAG_C
Cuts at: 0 259 619 948
Size: 259 360 329
Fragments arranged by size:
360 329 259
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 540 948
Size: 540 408
BsiHKAI G_wGCw'C
Cuts at: 0 155 374 948
Size: 155 219 574
Fragments arranged by size:
574 219 155
Bsp1286I G_dGCh'C
Cuts at: 0 137 155 192 374 620 882 948
Size: 137 18 37 182 246 262 66
Fragments arranged by size:
262 246 182 137 66 37 18
BsrDI GCAATG_nn'
Cuts at: 0 665 948
Size: 665 283
BstYI r'GATC_y
Cuts at: 0 501 948
Size: 501 447
BtgI C'CryG_G
Cuts at: 0 139 948
Size: 139 809
BtsI GCAGTG_nn'
Cuts at: 0 552 948
Size: 552 396
DrdI GACnn_nn'nnGTC
Cuts at: 0 18 948
Size: 18 930
EaeI y'GGCC_r
Cuts at: 0 315 948
Size: 315 633
EarI CTCTTCn'nnn_
Cuts at: 0 639 920 948
Size: 639 281 28
Fragments arranged by size:
639 281 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 139 358 409 948
Size: 139 219 51 539
Fragments arranged by size:
539 219 139 51
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 395 427 903 935 948
Size: 395 32 476 32 13
Fragments arranged by size:
476 395 32 32 13
HincII GTy'rAC
Cuts at: 0 936 948
Size: 936 12
MscI TGG'CCA
Cuts at: 0 317 948
Size: 317 631
MslI CAynn'nnrTG
Cuts at: 0 542 948
Size: 542 406
MspA1I CmG'CkG
Cuts at: 0 293 431 847 948
Size: 293 138 416 101
Fragments arranged by size:
416 293 138 101
NspI r_CATG'y
Cuts at: 0 182 948
Size: 182 766
PasI CC'CwG_GG
Cuts at: 0 875 948
Size: 875 73
PflMI CCAn_nnn'nTGG
Cuts at: 0 218 948
Size: 218 730
PfoI T'CCnGG_A
Cuts at: 0 155 395 948
Size: 155 240 553
Fragments arranged by size:
553 240 155
PmlI CAC'GTG
Cuts at: 0 305 948
Size: 305 643
PstI C_TGCA'G
Cuts at: 0 93 721 948
Size: 93 628 227
Fragments arranged by size:
628 227 93
PvuII CAG'CTG
Cuts at: 0 431 847 948
Size: 431 416 101
Fragments arranged by size:
431 416 101
SapI GCTCTTCn'nnn_
Cuts at: 0 639 948
Size: 639 309
SfcI C'TryA_G
Cuts at: 0 89 264 677 698 717 948
Size: 89 175 413 21 19 231
Fragments arranged by size:
413 231 175 89 21 19
SmlI C'TyrA_G
Cuts at: 0 130 948
Size: 130 818
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 262 948
Size: 262 686
TatI w'GTAC_w
Cuts at: 0 299 948
Size: 299 649
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 289 321 948
Size: 289 32 627
Fragments arranged by size:
627 289 32
Tth111I GACn'n_nGTC
Cuts at: 0 931 948
Size: 931 17
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 538 948
Size: 538 410
ZraI GAC'GTC
Cuts at: 0 787 948
Size: 787 161
Enzymes that do cut:
AatII AflIII AleI ApoI BamHI BanI BanII BbvCI
BglI Bme1580I BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaXI BseYI BsgI BsiHKAI Bsp1286I BsrDI
BstYI BtgI BtsI DrdI EaeI EarI Eco57MI Hin4I
HincII MscI MslI MspA1I NspI PasI PflMI PfoI
PmlI PstI PvuII SapI SfcI SmlI TaqII TatI
TstI Tth111I XcmI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI ApaLI AscI AseI
AsiSI AvaI AvrII BaeI BbeI BbsI BcgI BciVI
BclI BfrBI BglII BlpI BmgBI BmrI BmtI BsaWI
BseRI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstXI BstZ17I Bsu36I BtgZI ClaI CspCI DraI DraIII
EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV FalI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PmeI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI RsrII
SacI SacII SalI SanDI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI StuI StyI SwaI XbaI XhoI XmaI XmnI