Restriction map for
pF1KB3486
(Linear) (Six-base) MAPSORT of: KIBB3486.seq Check: 6617 from: 1 to: 1104
KIBB3486.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 621 1104
Size: 621 483
AatII G_ACGT'C
Cuts at: 0 995 1104
Size: 995 109
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 472 493 1104
Size: 37 435 21 611
Fragments arranged by size:
611 435 37 21
AhdI GACnn_n'nnGTC
Cuts at: 0 1050 1104
Size: 1050 54
AlwNI CAG_nnn'CTG
Cuts at: 0 435 558 696 1104
Size: 435 123 138 408
Fragments arranged by size:
435 408 138 123
AvaI C'yCGr_G
Cuts at: 0 776 955 1104
Size: 776 179 149
Fragments arranged by size:
776 179 149
BanII G_rGCy'C
Cuts at: 0 785 956 1104
Size: 785 171 148
Fragments arranged by size:
785 171 148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 839 873 1104
Size: 839 34 231
Fragments arranged by size:
839 231 34
BglI GCCn_nnn'nGGC
Cuts at: 0 193 1055 1104
Size: 193 862 49
Fragments arranged by size:
862 193 49
BlpI GC'TnA_GC
Cuts at: 0 197 1069 1104
Size: 197 872 35
Fragments arranged by size:
872 197 35
Bme1580I G_kGCm'C
Cuts at: 0 1094 1104
Size: 1094 10
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 502 580 634 1104
Size: 412 90 78 54 470
Fragments arranged by size:
470 412 90 78 54
Bpu10I CC'TnA_GC
Cuts at: 0 752 803 1104
Size: 752 51 301
Fragments arranged by size:
752 301 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 703 1104
Size: 703 401
BsaAI yAC'GTr
Cuts at: 0 1077 1104
Size: 1077 27
BsaHI Gr'CG_yC
Cuts at: 0 992 1104
Size: 992 112
BsaWI w'CCGG_w
Cuts at: 0 887 1104
Size: 887 217
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 556 586 1104
Size: 556 30 518
Fragments arranged by size:
556 518 30
BseYI C'CCAG_C
Cuts at: 0 183 309 972 1104
Size: 183 126 663 132
Fragments arranged by size:
663 183 132 126
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 813 1104
Size: 709 104 291
Fragments arranged by size:
709 291 104
Bsp1286I G_dGCh'C
Cuts at: 0 785 956 1094 1104
Size: 785 171 138 10
Fragments arranged by size:
785 171 138 10
BspEI T'CCGG_A
Cuts at: 0 887 1104
Size: 887 217
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 179 621 1104
Size: 179 442 483
Fragments arranged by size:
483 442 179
BsrBI CCG'CTC
Cuts at: 0 1068 1104
Size: 1068 36
BsrDI GCAATG_nn'
Cuts at: 0 337 1104
Size: 337 767
BsrFI r'CCGG_y
Cuts at: 0 349 677 1104
Size: 349 328 427
Fragments arranged by size:
427 349 328
BstAPI GCAn_nnn'nTGC
Cuts at: 0 687 1104
Size: 687 417
BstXI CCAn_nnnn'nTGG
Cuts at: 0 585 1104
Size: 585 519
BstYI r'GATC_y
Cuts at: 0 227 458 530 692 1104
Size: 227 231 72 162 412
Fragments arranged by size:
412 231 227 162 72
BtgI C'CryG_G
Cuts at: 0 297 1104
Size: 297 807
ClaI AT'CG_AT
Cuts at: 0 1016 1104
Size: 1016 88
DrdI GACnn_nn'nnGTC
Cuts at: 0 400 1104
Size: 400 704
EaeI y'GGCC_r
Cuts at: 0 152 1047 1104
Size: 152 895 57
Fragments arranged by size:
895 152 57
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 283 724 1104
Size: 283 441 380
Fragments arranged by size:
441 380 283
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 37 412 472 493 502 580 634 1104
Size: 37 375 60 21 9 78 54 470
Fragments arranged by size:
470 375 78 60 54 37 21 9
EcoNI CCTnn'n_nnAGG
Cuts at: 0 373 1104
Size: 373 731
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 429 1104
Size: 429 675
HaeII r_GCGC'y
Cuts at: 0 369 559 940 1104
Size: 369 190 381 164
Fragments arranged by size:
381 369 190 164
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1036 1068 1104
Size: 1036 32 36
Fragments arranged by size:
1036 36 32
HindIII A'AGCT_T
Cuts at: 0 737 1104
Size: 737 367
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 42 245 347 836 899 1104
Size: 42 203 102 489 63 205
Fragments arranged by size:
489 205 203 102 63 42
MscI TGG'CCA
Cuts at: 0 154 1104
Size: 154 950
MspA1I CmG'CkG
Cuts at: 0 45 666 1104
Size: 45 621 438
Fragments arranged by size:
621 438 45
NaeI GCC'GGC
Cuts at: 0 351 1104
Size: 351 753
NgoMIV G'CCGG_C
Cuts at: 0 349 1104
Size: 349 755
NspI r_CATG'y
Cuts at: 0 603 909 1041 1104
Size: 603 306 132 63
Fragments arranged by size:
603 306 132 63
PflMI CCAn_nnn'nTGG
Cuts at: 0 558 1104
Size: 558 546
PfoI T'CCnGG_A
Cuts at: 0 611 695 1104
Size: 611 84 409
Fragments arranged by size:
611 409 84
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 238 270 1104
Size: 238 32 834
Fragments arranged by size:
834 238 32
PstI C_TGCA'G
Cuts at: 0 176 1104
Size: 176 928
PvuII CAG'CTG
Cuts at: 0 45 666 1104
Size: 45 621 438
Fragments arranged by size:
621 438 45
SfcI C'TryA_G
Cuts at: 0 172 1104
Size: 172 932
SmaI CCC'GGG
Cuts at: 0 778 1104
Size: 778 326
SmlI C'TyrA_G
Cuts at: 0 718 1104
Size: 718 386
SphI G_CATG'C
Cuts at: 0 603 909 1041 1104
Size: 603 306 132 63
Fragments arranged by size:
603 306 132 63
StyI C'CwwG_G
Cuts at: 0 578 792 1104
Size: 578 214 312
Fragments arranged by size:
578 312 214
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 366 1104
Size: 366 738
XmaI C'CCGG_G
Cuts at: 0 776 1104
Size: 776 328
XmnI GAAnn'nnTTC
Cuts at: 0 25 1104
Size: 25 1079
ZraI GAC'GTC
Cuts at: 0 993 1104
Size: 993 111
Enzymes that do cut:
AarI AatII AcuI AhdI AlwNI AvaI BanII BcgI
BglI BlpI Bme1580I BpmI Bpu10I BpuEI BsaAI BsaHI
BsaWI BseRI BseYI BsgI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BstAPI BstXI BstYI BtgI ClaI DrdI
EaeI EciI Eco57MI EcoNI FalI HaeII Hin4I HindIII
MmeI MscI MspA1I NaeI NgoMIV NspI PflMI PfoI
PsrI PstI PvuII SfcI SmaI SmlI SphI StyI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AccI Acc65I AclI AfeI AflII AflIII AgeI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BaeI BamHI BanI BbeI BbsI BbvCI BciVI
BclI BfrBI BglII BmgBI BmrI BmtI BplI BsaI
BsaBI BsaXI BsiEI BsiHKAI BsiWI BsmI BsmBI BspHI
BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtgZI
BtsI CspCI DraI DraIII EagI EarI EcoICRI EcoO109I
EcoRI EcoRV FseI FspI FspAI HincII HpaI KasI
KpnI MfeI MluI MslI NarI NcoI NdeI NheI
NotI NruI NsiI PacI PasI PciI PmeI PmlI
PpiI PpuMI PshAI PsiI PspOMI PspXI PvuI RsrII
SacI SacII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SnaBI SpeI SrfI SspI
StuI SwaI TaqII TatI TstI Tth111I XbaI XhoI