Restriction map for
pF1KB3487
(Linear) (Six-base) MAPSORT of: KIBB3487.seq Check: 2209 from: 1 to: 1992
KIBB3487.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 0 1184 1992
Size: 1184 808
AccI GT'mk_AC
Cuts at: 0 1091 1992
Size: 1091 901
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 774 1666 1992
Size: 509 265 892 326
Fragments arranged by size:
892 509 326 265
AfeI AGC'GCT
Cuts at: 0 36 1992
Size: 36 1956
AhdI GACnn_n'nnGTC
Cuts at: 0 1011 1992
Size: 1011 981
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1580 1992
Size: 1580 412
AlwNI CAG_nnn'CTG
Cuts at: 0 1263 1710 1992
Size: 1263 447 282
Fragments arranged by size:
1263 447 282
ApoI r'AATT_y
Cuts at: 0 181 1992
Size: 181 1811
AvaI C'yCGr_G
Cuts at: 0 89 1354 1992
Size: 89 1265 638
Fragments arranged by size:
1265 638 89
BanI G'GyrC_C
Cuts at: 0 41 1342 1992
Size: 41 1301 650
Fragments arranged by size:
1301 650 41
BanII G_rGCy'C
Cuts at: 0 388 1992
Size: 388 1604
BbeI G_GCGC'C
Cuts at: 0 45 1992
Size: 45 1947
BbsI GAAGACnn'nnnn_
Cuts at: 0 1654 1790 1992
Size: 1654 136 202
Fragments arranged by size:
1654 202 136
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 541 575 1992
Size: 541 34 1417
Fragments arranged by size:
1417 541 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 173 1992
Size: 173 1819
BclI T'GATC_A
Cuts at: 0 836 1992
Size: 836 1156
BglI GCCn_nnn'nGGC
Cuts at: 0 1142 1992
Size: 1142 850
BlpI GC'TnA_GC
Cuts at: 0 31 1992
Size: 31 1961
Bme1580I G_kGCm'C
Cuts at: 0 1298 1404 1992
Size: 1298 106 588
Fragments arranged by size:
1298 588 106
BmgBI CAC'GTC
Cuts at: 0 800 1089 1992
Size: 800 289 903
Fragments arranged by size:
903 800 289
BmrI ACTGGGnnnn_n'
Cuts at: 0 1910 1992
Size: 1910 82
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 967 1992
Size: 967 1025
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 375 1066 1100 1451 1717 1884 1992
Size: 227 148 691 34 351 266 167 108
Fragments arranged by size:
691 351 266 227 167 148 108 34
Bpu10I CC'TnA_GC
Cuts at: 0 1405 1992
Size: 1405 587
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 517 1992
Size: 517 1475
BsaI GGTCTCn'nnnn_
Cuts at: 0 626 1625 1684 1992
Size: 626 999 59 308
Fragments arranged by size:
999 626 308 59
BsaAI yAC'GTr
Cuts at: 0 1272 1992
Size: 1272 720
BsaHI Gr'CG_yC
Cuts at: 0 42 1012 1181 1992
Size: 42 970 169 811
Fragments arranged by size:
970 811 169 42
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1424 1454 1801 1831 1992
Size: 1424 30 347 30 161
Fragments arranged by size:
1424 347 161 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 358 1870 1992
Size: 358 1512 122
Fragments arranged by size:
1512 358 122
BseYI C'CCAG_C
Cuts at: 0 396 1462 1992
Size: 396 1066 530
Fragments arranged by size:
1066 530 396
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 780 1087 1992
Size: 780 307 905
Fragments arranged by size:
905 780 307
BsiEI CG_ry'CG
Cuts at: 0 1036 1659 1992
Size: 1036 623 333
Fragments arranged by size:
1036 623 333
BsiHKAI G_wGCw'C
Cuts at: 0 1862 1992
Size: 1862 130
BsmI GAATG_Cn'
Cuts at: 0 947 1235 1992
Size: 947 288 757
Fragments arranged by size:
947 757 288
Bsp1286I G_dGCh'C
Cuts at: 0 388 1298 1404 1862 1992
Size: 388 910 106 458 130
Fragments arranged by size:
910 458 388 130 106
BspHI T'CATG_A
Cuts at: 0 177 1478 1992
Size: 177 1301 514
Fragments arranged by size:
1301 514 177
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1103 1248 1992
Size: 1103 145 744
Fragments arranged by size:
1103 744 145
BssHII G'CGCG_C
Cuts at: 0 1769 1992
Size: 1769 223
BstYI r'GATC_y
Cuts at: 0 666 1049 1992
Size: 666 383 943
Fragments arranged by size:
943 666 383
Bsu36I CC'TnA_GG
Cuts at: 0 1264 1992
Size: 1264 728
BtgI C'CryG_G
Cuts at: 0 446 693 1992
Size: 446 247 1299
Fragments arranged by size:
1299 446 247
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 687 718 1992
Size: 687 31 1274
Fragments arranged by size:
1274 687 31
BtsI GCAGTG_nn'
Cuts at: 0 197 1992
Size: 197 1795
DrdI GACnn_nn'nnGTC
Cuts at: 0 1381 1992
Size: 1381 611
EaeI y'GGCC_r
Cuts at: 0 53 64 588 830 1127 1278 1992
Size: 53 11 524 242 297 151 714
Fragments arranged by size:
714 524 297 242 151 53 11
EarI CTCTTCn'nnn_
Cuts at: 0 1056 1409 1992
Size: 1056 353 583
Fragments arranged by size:
1056 583 353
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 227 375 509 774 1066 1100 1451 1666
Size: 227 148 134 265 292 34 351 215
Cuts at: 1666 1717 1884 1992
Size: 51 167 108
Fragments arranged by size:
351 292 265 227 215 167 148 134
108 51 34
EcoNI CCTnn'n_nnAGG
Cuts at: 0 241 1992
Size: 241 1751
EcoO109I rG'GnC_Cy
Cuts at: 0 15 435 864 1005 1260 1992
Size: 15 420 429 141 255 732
Fragments arranged by size:
732 429 420 255 141 15
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1866 1992
Size: 1866 126
HaeII r_GCGC'y
Cuts at: 0 38 45 52 206 1992
Size: 38 7 7 154 1786
Fragments arranged by size:
1786 154 38 7 7
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 515 547 709 741 997 1012 1029 1044
Size: 515 32 162 32 256 15 17 15
Cuts at: 1044 1424 1456 1916 1948 1992
Size: 380 32 460 32 44
Fragments arranged by size:
515 460 380 256 162 44 32 32
32 32 17 15 15
KasI G'GCGC_C
Cuts at: 0 41 1992
Size: 41 1951
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 413 1992
Size: 413 1579
MscI TGG'CCA
Cuts at: 0 55 66 590 1129 1280 1992
Size: 55 11 524 539 151 712
Fragments arranged by size:
712 539 524 151 55 11
MslI CAynn'nnrTG
Cuts at: 0 832 1274 1992
Size: 832 442 718
Fragments arranged by size:
832 718 442
MspA1I CmG'CkG
Cuts at: 0 794 1101 1125 1992
Size: 794 307 24 867
Fragments arranged by size:
867 794 307 24
NarI GG'CG_CC
Cuts at: 0 42 1992
Size: 42 1950
NruI TCG'CGA
Cuts at: 0 1000 1992
Size: 1000 992
NspI r_CATG'y
Cuts at: 0 266 474 1400 1545 1777 1992
Size: 266 208 926 145 232 215
Fragments arranged by size:
926 266 232 215 208 145
PflMI CCAn_nnn'nTGG
Cuts at: 0 62 1462 1992
Size: 62 1400 530
Fragments arranged by size:
1400 530 62
PmlI CAC'GTG
Cuts at: 0 1272 1992
Size: 1272 720
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1520 1552 1992
Size: 1520 32 440
Fragments arranged by size:
1520 440 32
PpuMI rG'GwC_Cy
Cuts at: 0 864 1005 1260 1992
Size: 864 141 255 732
Fragments arranged by size:
864 732 255 141
PsiI TTA'TAA
Cuts at: 0 1223 1992
Size: 1223 769
PstI C_TGCA'G
Cuts at: 0 1100 1706 1730 1992
Size: 1100 606 24 262
Fragments arranged by size:
1100 606 262 24
PvuII CAG'CTG
Cuts at: 0 794 1101 1125 1992
Size: 794 307 24 867
Fragments arranged by size:
867 794 307 24
SexAI A'CCwGG_T
Cuts at: 0 286 1376 1682 1992
Size: 286 1090 306 310
Fragments arranged by size:
1090 310 306 286
SfcI C'TryA_G
Cuts at: 0 1096 1702 1726 1992
Size: 1096 606 24 266
Fragments arranged by size:
1096 606 266 24
SfoI GGC'GCC
Cuts at: 0 43 1992
Size: 43 1949
SmlI C'TyrA_G
Cuts at: 0 532 1992
Size: 532 1460
SphI G_CATG'C
Cuts at: 0 474 1545 1777 1992
Size: 474 1071 232 215
Fragments arranged by size:
1071 474 232 215
StuI AGG'CCT
Cuts at: 0 851 1428 1992
Size: 851 577 564
Fragments arranged by size:
851 577 564
StyI C'CwwG_G
Cuts at: 0 56 662 1992
Size: 56 606 1330
Fragments arranged by size:
1330 606 56
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1022 1992
Size: 1022 970
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1864 1896 1992
Size: 1864 32 96
Fragments arranged by size:
1864 96 32
XbaI T'CTAG_A
Cuts at: 0 1015 1992
Size: 1015 977
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 645 814 1992
Size: 645 169 1178
Fragments arranged by size:
1178 645 169
XmnI GAAnn'nnTTC
Cuts at: 0 1651 1992
Size: 1651 341
ZraI GAC'GTC
Cuts at: 0 1182 1992
Size: 1182 810
Enzymes that do cut:
AatII AccI AcuI AfeI AhdI AlfI AlwNI ApoI
AvaI BanI BanII BbeI BbsI BcgI BciVI BclI
BglI BlpI Bme1580I BmgBI BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspHI BspMI BssHII BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I FalI HaeII Hin4I KasI MmeI MscI
MslI MspA1I NarI NruI NspI PflMI PmlI PpiI
PpuMI PsiI PstI PvuII SexAI SfcI SfoI SmlI
SphI StuI StyI TaqII TstI XbaI XcmI XmnI
ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AleI AloI
ApaI ApaLI AscI AseI AsiSI AvrII BaeI BamHI
BbvCI BfrBI BglII BmtI BsaBI BsaWI BsiWI BsmBI
BspEI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII BstXI BstZ17I ClaI CspCI DraI DraIII EagI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI HincII
HindIII HpaI KpnI MfeI MluI NaeI NcoI NdeI
NgoMIV NheI NotI NsiI PacI PasI PciI PfoI
PmeI PshAI PspOMI PspXI PsrI PvuI RsrII SacI
SacII SalI SanDI SapI SbfI ScaI SfiI SgfI
SgrAI SmaI SnaBI SpeI SrfI SspI SwaI TatI
Tth111I XhoI XmaI