Restriction map for
pF1KB3488
(Linear) (Six-base) MAPSORT of: KIBB3488.seq Check: 6509 from: 1 to: 1050
KIBB3488.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AclI AA'CG_TT
Cuts at: 0 611 1050
Size: 611 439
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1039 1050
Size: 1039 11
AleI CACnn'nnGTG
Cuts at: 0 209 1050
Size: 209 841
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 549 581 1050
Size: 549 32 469
Fragments arranged by size:
549 469 32
AlwNI CAG_nnn'CTG
Cuts at: 0 925 1050
Size: 925 125
ApoI r'AATT_y
Cuts at: 0 342 1050
Size: 342 708
AseI AT'TA_AT
Cuts at: 0 479 1050
Size: 479 571
AvrII C'CTAG_G
Cuts at: 0 573 1050
Size: 573 477
BbvCI CC'TCA_GC
Cuts at: 0 994 1050
Size: 994 56
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 217 251 1050
Size: 217 34 799
Fragments arranged by size:
799 217 34
BclI T'GATC_A
Cuts at: 0 776 791 1050
Size: 776 15 259
Fragments arranged by size:
776 259 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 902 947 1050
Size: 431 471 45 103
Fragments arranged by size:
471 431 103 45
Bpu10I CC'TnA_GC
Cuts at: 0 994 1050
Size: 994 56
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 46 76 794 824 1050
Size: 46 30 718 30 226
Fragments arranged by size:
718 226 46 30 30
BseYI C'CCAG_C
Cuts at: 0 137 904 1050
Size: 137 767 146
Fragments arranged by size:
767 146 137
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 622 1050
Size: 622 428
BsmI GAATG_Cn'
Cuts at: 0 563 623 1050
Size: 563 60 427
Fragments arranged by size:
563 427 60
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 751 1050
Size: 751 299
BsrDI GCAATG_nn'
Cuts at: 0 441 623 635 959 1050
Size: 441 182 12 324 91
Fragments arranged by size:
441 324 182 91 12
BsrFI r'CCGG_y
Cuts at: 0 519 1050
Size: 519 531
BstAPI GCAn_nnn'nTGC
Cuts at: 0 156 1050
Size: 156 894
BstBI TT'CG_AA
Cuts at: 0 99 1050
Size: 99 951
BstXI CCAn_nnnn'nTGG
Cuts at: 0 948 1050
Size: 948 102
BstYI r'GATC_y
Cuts at: 0 428 1050
Size: 428 622
Bsu36I CC'TnA_GG
Cuts at: 0 186 1050
Size: 186 864
BtgI C'CryG_G
Cuts at: 0 529 1050
Size: 529 521
ClaI AT'CG_AT
Cuts at: 0 696 1050
Size: 696 354
EaeI y'GGCC_r
Cuts at: 0 199 1050
Size: 199 851
EarI CTCTTCn'nnn_
Cuts at: 0 167 407 998 1050
Size: 167 240 591 52
Fragments arranged by size:
591 240 167 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 431 902 947 1039 1050
Size: 431 471 45 92 11
Fragments arranged by size:
471 431 92 45 11
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 794 826 1050
Size: 794 32 224
Fragments arranged by size:
794 224 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 594 849 1050
Size: 592 2 255 201
Fragments arranged by size:
592 255 201 2
MslI CAynn'nnrTG
Cuts at: 0 209 248 416 628 644 817 946 1050
Size: 209 39 168 212 16 173 129 104
Fragments arranged by size:
212 209 173 168 129 104 39 16
MspA1I CmG'CkG
Cuts at: 0 59 274 1050
Size: 59 215 776
Fragments arranged by size:
776 215 59
NcoI C'CATG_G
Cuts at: 0 529 1050
Size: 529 521
PflMI CCAn_nnn'nTGG
Cuts at: 0 925 1050
Size: 925 125
PfoI T'CCnGG_A
Cuts at: 0 663 924 1050
Size: 663 261 126
Fragments arranged by size:
663 261 126
PstI C_TGCA'G
Cuts at: 0 47 279 762 1050
Size: 47 232 483 288
Fragments arranged by size:
483 288 232 47
PvuII CAG'CTG
Cuts at: 0 59 274 1050
Size: 59 215 776
Fragments arranged by size:
776 215 59
SapI GCTCTTCn'nnn_
Cuts at: 0 998 1050
Size: 998 52
SfcI C'TryA_G
Cuts at: 0 43 275 758 1050
Size: 43 232 483 292
Fragments arranged by size:
483 292 232 43
StyI C'CwwG_G
Cuts at: 0 529 573 1050
Size: 529 44 477
Fragments arranged by size:
529 477 44
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 168 1050
Size: 168 882
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 46 78 906 938 1050
Size: 46 32 828 32 112
Fragments arranged by size:
828 112 46 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 536 861 1050
Size: 536 325 189
Fragments arranged by size:
536 325 189
Enzymes that do cut:
AclI AcuI AleI AloI AlwNI ApoI AseI AvrII
BbvCI BcgI BclI BpmI Bpu10I BsaXI BseYI BsgI
BsmI BspMI BsrDI BsrFI BstAPI BstBI BstXI BstYI
Bsu36I BtgI ClaI EaeI EarI Eco57MI Hin4I MmeI
MslI MspA1I NcoI PflMI PfoI PstI PvuII SapI
SfcI StyI TaqII TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AfeI AflII AflIII AgeI
AhdI AlfI ApaI ApaLI AscI AsiSI AvaI BaeI
BamHI BanI BanII BbeI BbsI BciVI BfrBI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpuEI
BsaI BsaAI BsaBI BsaHI BsaWI BseRI BsiEI BsiHKAI
BsiWI BsmBI Bsp1286I BspEI BspHI BsrBI BsrGI BssHII
BssSI BstEII BstZ17I BtgZI BtsI CspCI DraI DraIII
DrdI EagI EciI EcoICRI EcoNI EcoO109I EcoRI EcoRV
FalI FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MfeI MluI MscI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI NspI PacI PasI
PciI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TatI Tth111I XbaI XhoI XmaI XmnI ZraI