Restriction map for
pF1KB3490
(Linear) (Six-base) MAPSORT of: KIBB3490.seq Check: 7825 from: 1 to: 1011
KIBB3490.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 718 1011
Size: 718 293
AccI GT'mk_AC
Cuts at: 0 704 1011
Size: 704 307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 826 860 1011
Size: 260 566 34 151
Fragments arranged by size:
566 260 151 34
AflIII A'CryG_T
Cuts at: 0 561 1011
Size: 561 450
AlwNI CAG_nnn'CTG
Cuts at: 0 90 452 1011
Size: 90 362 559
Fragments arranged by size:
559 362 90
ApoI r'AATT_y
Cuts at: 0 244 430 1011
Size: 244 186 581
Fragments arranged by size:
581 244 186
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 832 865 1011
Size: 832 33 146
Fragments arranged by size:
832 146 33
BanI G'GyrC_C
Cuts at: 0 719 934 1011
Size: 719 215 77
Fragments arranged by size:
719 215 77
BanII G_rGCy'C
Cuts at: 0 648 961 1011
Size: 648 313 50
Fragments arranged by size:
648 313 50
BbsI GAAGACnn'nnnn_
Cuts at: 0 155 1011
Size: 155 856
BbvCI CC'TCA_GC
Cuts at: 0 9 1011
Size: 9 1002
BlpI GC'TnA_GC
Cuts at: 0 955 1011
Size: 955 56
Bme1580I G_kGCm'C
Cuts at: 0 722 1011
Size: 722 289
BmtI G_CTAG'C
Cuts at: 0 329 1011
Size: 329 682
Bpu10I CC'TnA_GC
Cuts at: 0 9 316 1011
Size: 9 307 695
Fragments arranged by size:
695 307 9
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1 432 712 1011
Size: 1 431 280 299
Fragments arranged by size:
431 299 280 1
BsaI GGTCTCn'nnnn_
Cuts at: 0 983 1011
Size: 983 28
BsaHI Gr'CG_yC
Cuts at: 0 383 398 1011
Size: 383 15 613
Fragments arranged by size:
613 383 15
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 709 1011
Size: 709 302
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 489 696 1011
Size: 489 207 315
Fragments arranged by size:
489 315 207
BsiHKAI G_wGCw'C
Cuts at: 0 667 961 1011
Size: 667 294 50
Fragments arranged by size:
667 294 50
BsmI GAATG_Cn'
Cuts at: 0 276 411 1011
Size: 276 135 600
Fragments arranged by size:
600 276 135
Bsp1286I G_dGCh'C
Cuts at: 0 648 667 722 961 1011
Size: 648 19 55 239 50
Fragments arranged by size:
648 239 55 50 19
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 65 718 1011
Size: 65 653 293
Fragments arranged by size:
653 293 65
BsrBI CCG'CTC
Cuts at: 0 726 1011
Size: 726 285
BsrDI GCAATG_nn'
Cuts at: 0 737 965 1011
Size: 737 228 46
Fragments arranged by size:
737 228 46
BsrFI r'CCGG_y
Cuts at: 0 77 1011
Size: 77 934
BssSI C'ACGA_G
Cuts at: 0 507 1011
Size: 507 504
BstBI TT'CG_AA
Cuts at: 0 882 1011
Size: 882 129
BstXI CCAn_nnnn'nTGG
Cuts at: 0 261 750 1011
Size: 261 489 261
Fragments arranged by size:
489 261 261
BstYI r'GATC_y
Cuts at: 0 947 1011
Size: 947 64
BtgI C'CryG_G
Cuts at: 0 35 254 1011
Size: 35 219 757
Fragments arranged by size:
757 219 35
BtsI GCAGTG_nn'
Cuts at: 0 817 1011
Size: 817 194
EaeI y'GGCC_r
Cuts at: 0 79 1011
Size: 79 932
EarI CTCTTCn'nnn_
Cuts at: 0 411 569 1011
Size: 411 158 442
Fragments arranged by size:
442 411 158
EcoICRI GAG'CTC
Cuts at: 0 959 1011
Size: 959 52
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 260 826 860 1011
Size: 260 566 34 151
Fragments arranged by size:
566 260 151 34
EcoRV GAT'ATC
Cuts at: 0 828 1011
Size: 828 183
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 369 1011
Size: 369 642
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 296 328 440 472 750 782 856 888
Size: 296 32 112 32 278 32 74 32
Cuts at: 888 1011
Size: 123
Fragments arranged by size:
296 278 123 112 74 32 32 32
32
HincII GTy'rAC
Cuts at: 0 864 1011
Size: 864 147
MslI CAynn'nnrTG
Cuts at: 0 670 1011
Size: 670 341
MspA1I CmG'CkG
Cuts at: 0 37 503 954 1011
Size: 37 466 451 57
Fragments arranged by size:
466 451 57 37
NcoI C'CATG_G
Cuts at: 0 254 1011
Size: 254 757
NheI G'CTAG_C
Cuts at: 0 325 1011
Size: 325 686
NspI r_CATG'y
Cuts at: 0 565 639 1011
Size: 565 74 372
Fragments arranged by size:
565 372 74
PasI CC'CwG_GG
Cuts at: 0 62 184 1011
Size: 62 122 827
Fragments arranged by size:
827 122 62
PciI A'CATG_T
Cuts at: 0 561 1011
Size: 561 450
PfoI T'CCnGG_A
Cuts at: 0 749 1011
Size: 749 262
PvuII CAG'CTG
Cuts at: 0 954 1011
Size: 954 57
SacI G_AGCT'C
Cuts at: 0 961 1011
Size: 961 50
SacII CC_GC'GG
Cuts at: 0 38 1011
Size: 38 973
SfcI C'TryA_G
Cuts at: 0 786 1011
Size: 786 225
SmlI C'TyrA_G
Cuts at: 0 16 411 727 1011
Size: 16 395 316 284
Fragments arranged by size:
395 316 284 16
SpeI A'CTAG_T
Cuts at: 0 612 1011
Size: 612 399
SspI AAT'ATT
Cuts at: 0 341 1011
Size: 341 670
StyI C'CwwG_G
Cuts at: 0 254 1011
Size: 254 757
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 167 299 324 511 1011
Size: 167 132 25 187 500
Fragments arranged by size:
500 187 167 132 25
TatI w'GTAC_w
Cuts at: 0 917 1011
Size: 917 94
Enzymes that do cut:
AarI AccI AcuI AflIII AlwNI ApoI BaeI BanI
BanII BbsI BbvCI BlpI Bme1580I BmtI Bpu10I BpuEI
BsaI BsaHI BseRI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrDI BsrFI BssSI BstBI BstXI BstYI BtgI
BtsI EaeI EarI EcoICRI Eco57MI EcoRV FalI Hin4I
HincII MslI MspA1I NcoI NheI NspI PasI PciI
PfoI PvuII SacI SacII SfcI SmlI SpeI SspI
StyI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BamHI BbeI BcgI BciVI BclI BfrBI BglI
BglII BmgBI BmrI BplI BpmI BsaAI BsaBI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BsrGI
BssHII BstAPI BstEII BstZ17I Bsu36I BtgZI ClaI CspCI
DraI DraIII DrdI EagI EciI EcoNI EcoO109I EcoRI
FseI FspI FspAI HaeII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI NaeI NarI NdeI NgoMIV
NotI NruI NsiI PacI PflMI PmeI PmlI PpiI
PpuMI PshAI PsiI PspOMI PspXI PsrI PstI PvuI
RsrII SalI SanDI SapI SbfI ScaI SexAI SfiI
SfoI SgfI SgrAI SmaI SnaBI SphI SrfI StuI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI