Restriction map for
pF1KB3493
(Linear) (Six-base) MAPSORT of: KIBB3493.seq Check: 4590 from: 1 to: 1890
KIBB3493.orf
With 182 enzymes: SgfI *
September 18, 2009 17:00 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 398 1890
Size: 398 1492
AccI GT'mk_AC
Cuts at: 0 167 965 1890
Size: 167 798 925
Fragments arranged by size:
925 798 167
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 1063 1890
Size: 352 711 827
Fragments arranged by size:
827 711 352
AhdI GACnn_n'nnGTC
Cuts at: 0 1110 1890
Size: 1110 780
AleI CACnn'nnGTG
Cuts at: 0 1726 1890
Size: 1726 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 27 1890
Size: 27 1863
AlwNI CAG_nnn'CTG
Cuts at: 0 228 894 1503 1890
Size: 228 666 609 387
Fragments arranged by size:
666 609 387 228
ApaI G_GGCC'C
Cuts at: 0 1612 1890
Size: 1612 278
ApoI r'AATT_y
Cuts at: 0 493 1890
Size: 493 1397
AvaI C'yCGr_G
Cuts at: 0 245 505 973 1890
Size: 245 260 468 917
Fragments arranged by size:
917 468 260 245
BamHI G'GATC_C
Cuts at: 0 1529 1890
Size: 1529 361
BanI G'GyrC_C
Cuts at: 0 1861 1890
Size: 1861 29
BanII G_rGCy'C
Cuts at: 0 56 136 1073 1224 1355 1612 1890
Size: 56 80 937 151 131 257 278
Fragments arranged by size:
937 278 257 151 131 80 56
BbsI GAAGACnn'nnnn_
Cuts at: 0 355 1890
Size: 355 1535
BglI GCCn_nnn'nGGC
Cuts at: 0 241 313 1890
Size: 241 72 1577
Fragments arranged by size:
1577 241 72
BglII A'GATC_T
Cuts at: 0 1670 1890
Size: 1670 220
Bme1580I G_kGCm'C
Cuts at: 0 16 151 1612 1890
Size: 16 135 1461 278
Fragments arranged by size:
1461 278 135 16
BmgBI CAC'GTC
Cuts at: 0 119 353 1890
Size: 119 234 1537
Fragments arranged by size:
1537 234 119
BmrI ACTGGGnnnn_n'
Cuts at: 0 1073 1548 1890
Size: 1073 475 342
Fragments arranged by size:
1073 475 342
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 789 1890
Size: 789 1101
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 613 943 1048 1115 1342 1378 1890
Size: 70 543 330 105 67 227 36 512
Fragments arranged by size:
543 512 330 227 105 70 67 36
Bpu10I CC'TnA_GC
Cuts at: 0 571 1349 1890
Size: 571 778 541
Fragments arranged by size:
778 571 541
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 829 1291 1890
Size: 829 462 599
Fragments arranged by size:
829 599 462
BsaI GGTCTCn'nnnn_
Cuts at: 0 760 1152 1318 1890
Size: 760 392 166 572
Fragments arranged by size:
760 572 392 166
BsaHI Gr'CG_yC
Cuts at: 0 271 646 758 1890
Size: 271 375 112 1132
Fragments arranged by size:
1132 375 271 112
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 19 49 1337 1367 1624 1654 1890
Size: 19 30 1288 30 257 30 236
Fragments arranged by size:
1288 257 236 30 30 30 19
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 40 811 832 892 1890
Size: 40 771 21 60 998
Fragments arranged by size:
998 771 60 40 21
BseYI C'CCAG_C
Cuts at: 0 295 473 1107 1198 1503 1890
Size: 295 178 634 91 305 387
Fragments arranged by size:
634 387 305 295 178 91
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 275 1495 1890
Size: 172 103 1220 395
Fragments arranged by size:
1220 395 172 103
BsiEI CG_ry'CG
Cuts at: 0 181 1468 1629 1890
Size: 181 1287 161 261
Fragments arranged by size:
1287 261 181 161
BsiHKAI G_wGCw'C
Cuts at: 0 40 389 512 657 1021 1073 1355 1890
Size: 40 349 123 145 364 52 282 535
Fragments arranged by size:
535 364 349 282 145 123 52 40
BsmI GAATG_Cn'
Cuts at: 0 309 1890
Size: 309 1581
BsmBI CGTCTCn'nnnn_
Cuts at: 0 109 1890
Size: 109 1781
Bsp1286I G_dGCh'C
Cuts at: 0 16 40 56 136 151 389 512 657
Size: 16 24 16 80 15 238 123 145
Cuts at: 657 1021 1073 1224 1355 1612 1890
Size: 364 52 151 131 257 278
Fragments arranged by size:
364 278 257 238 151 145 131 123
80 52 24 16 16 15
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 398 1890
Size: 398 1492
BsrFI r'CCGG_y
Cuts at: 0 66 1463 1601 1890
Size: 66 1397 138 289
Fragments arranged by size:
1397 289 138 66
BsrGI T'GTAC_A
Cuts at: 0 626 1890
Size: 626 1264
BstAPI GCAn_nnn'nTGC
Cuts at: 0 228 687 1890
Size: 228 459 1203
Fragments arranged by size:
1203 459 228
BstEII G'GTnAC_C
Cuts at: 0 69 1087 1890
Size: 69 1018 803
Fragments arranged by size:
1018 803 69
BstXI CCAn_nnnn'nTGG
Cuts at: 0 48 1320 1728 1890
Size: 48 1272 408 162
Fragments arranged by size:
1272 408 162 48
BstYI r'GATC_y
Cuts at: 0 860 1529 1670 1890
Size: 860 669 141 220
Fragments arranged by size:
860 669 220 141
Bsu36I CC'TnA_GG
Cuts at: 0 604 1890
Size: 604 1286
BtgI C'CryG_G
Cuts at: 0 1752 1890
Size: 1752 138
BtsI GCAGTG_nn'
Cuts at: 0 160 324 1054 1512 1890
Size: 160 164 730 458 378
Fragments arranged by size:
730 458 378 164 160
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1551 1586 1890
Size: 1551 35 304
Fragments arranged by size:
1551 304 35
DraI TTT'AAA
Cuts at: 0 1244 1890
Size: 1244 646
DraIII CAC_nnn'GTG
Cuts at: 0 80 1890
Size: 80 1810
EaeI y'GGCC_r
Cuts at: 0 20 84 1334 1465 1626 1890
Size: 20 64 1250 131 161 264
Fragments arranged by size:
1250 264 161 131 64 20
EagI C'GGCC_G
Cuts at: 0 1465 1626 1890
Size: 1465 161 264
Fragments arranged by size:
1465 264 161
EarI CTCTTCn'nnn_
Cuts at: 0 788 796 847 1890
Size: 788 8 51 1043
Fragments arranged by size:
1043 788 51 8
EcoICRI GAG'CTC
Cuts at: 0 1071 1353 1890
Size: 1071 282 537
Fragments arranged by size:
1071 537 282
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 70 352 613 943 1048 1063 1115 1342
Size: 70 282 261 330 105 15 52 227
Cuts at: 1342 1378 1890
Size: 36 512
Fragments arranged by size:
512 330 282 261 227 105 70 52
36 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 22 334 574 777 1890
Size: 22 312 240 203 1113
Fragments arranged by size:
1113 312 240 203 22
EcoO109I rG'GnC_Cy
Cuts at: 0 1608 1890
Size: 1608 282
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 372 768 1581 1890
Size: 372 396 813 309
Fragments arranged by size:
813 396 372 309
HaeII r_GCGC'y
Cuts at: 0 587 1640 1890
Size: 587 1053 250
Fragments arranged by size:
1053 587 250
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 16 48 916 948 1096 1128 1337 1369
Size: 16 32 868 32 148 32 209 32
Cuts at: 1369 1890
Size: 521
Fragments arranged by size:
868 521 209 148 32 32 32 32
16
HincII GTy'rAC
Cuts at: 0 810 1890
Size: 810 1080
HindIII A'AGCT_T
Cuts at: 0 1204 1890
Size: 1204 686
MscI TGG'CCA
Cuts at: 0 1336 1890
Size: 1336 554
MslI CAynn'nnrTG
Cuts at: 0 144 739 1726 1890
Size: 144 595 987 164
Fragments arranged by size:
987 595 164 144
MspA1I CmG'CkG
Cuts at: 0 186 432 1890
Size: 186 246 1458
Fragments arranged by size:
1458 246 186
NruI TCG'CGA
Cuts at: 0 449 1890
Size: 449 1441
PfoI T'CCnGG_A
Cuts at: 0 419 1064 1355 1379 1890
Size: 419 645 291 24 511
Fragments arranged by size:
645 511 419 291 24
PspOMI G'GGCC_C
Cuts at: 0 1608 1890
Size: 1608 282
PstI C_TGCA'G
Cuts at: 0 233 395 437 1269 1556 1890
Size: 233 162 42 832 287 334
Fragments arranged by size:
832 334 287 233 162 42
PvuII CAG'CTG
Cuts at: 0 186 1890
Size: 186 1704
SacI G_AGCT'C
Cuts at: 0 1073 1355 1890
Size: 1073 282 535
Fragments arranged by size:
1073 535 282
SbfI CC_TGCA'GG
Cuts at: 0 233 1890
Size: 233 1657
SfcI C'TryA_G
Cuts at: 0 229 391 433 1265 1552 1890
Size: 229 162 42 832 287 338
Fragments arranged by size:
832 338 287 229 162 42
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 241 1890
Size: 241 1649
SmaI CCC'GGG
Cuts at: 0 247 1890
Size: 247 1643
SmlI C'TyrA_G
Cuts at: 0 844 1306 1890
Size: 844 462 584
Fragments arranged by size:
844 584 462
StuI AGG'CCT
Cuts at: 0 553 1890
Size: 553 1337
StyI C'CwwG_G
Cuts at: 0 1313 1715 1890
Size: 1313 402 175
Fragments arranged by size:
1313 402 175
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 309 1890
Size: 309 1581
TatI w'GTAC_w
Cuts at: 0 626 1890
Size: 626 1264
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 19 51 1890
Size: 19 32 1839
Fragments arranged by size:
1839 32 19
Tth111I GACn'n_nGTC
Cuts at: 0 1786 1890
Size: 1786 104
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 588 1890
Size: 588 1302
XmaI C'CCGG_G
Cuts at: 0 245 1890
Size: 245 1645
XmnI GAAnn'nnTTC
Cuts at: 0 1564 1890
Size: 1564 326
Enzymes that do cut:
AarI AccI AcuI AhdI AleI AlfI AlwNI ApaI
ApoI AvaI BamHI BanI BanII BbsI BglI BglII
Bme1580I BmgBI BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaHI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI
BsmBI Bsp1286I BspMI BsrFI BsrGI BstAPI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DraI DraIII EaeI
EagI EarI EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII
Hin4I HincII HindIII MscI MslI MspA1I NruI PfoI
PspOMI PstI PvuII SacI SbfI SfcI SfiI SmaI
SmlI StuI StyI TaqII TatI TstI Tth111I XcmI
XmaI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AloI
ApaLI AscI AseI AsiSI AvrII BaeI BbeI BbvCI
BcgI BciVI BclI BfrBI BlpI BmtI BsaAI BsaBI
BsaWI BsiWI BspEI BspHI BsrBI BsrDI BssHII BssSI
BstBI BstZ17I BtgZI ClaI DrdI EciI EcoRI EcoRV
FseI FspI FspAI HpaI KasI KpnI MfeI MluI
MmeI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NsiI NspI PacI PasI PciI PflMI PmeI PmlI
PpiI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SanDI SapI ScaI SexAI SfoI SgfI
SgrAI SnaBI SpeI SphI SrfI SspI SwaI XbaI
XhoI ZraI