Restriction map for
pF1KB3499
(Linear) (Six-base) MAPSORT of: KIBB3499.seq Check: 29 from: 1 to: 3216
KIBB3499.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1217 1607 1678 1831 3216
Size: 1217 390 71 153 1385
Fragments arranged by size:
1385 1217 390 153 71
AatII G_ACGT'C
Cuts at: 0 16 3216
Size: 16 3200
AccI GT'mk_AC
Cuts at: 0 362 434 827 3216
Size: 362 72 393 2389
Fragments arranged by size:
2389 393 362 72
Acc65I G'GTAC_C
Cuts at: 0 909 3216
Size: 909 2307
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2356 2911 3216
Size: 2356 555 305
Fragments arranged by size:
2356 555 305
AflIII A'CryG_T
Cuts at: 0 1181 2355 3216
Size: 1181 1174 861
Fragments arranged by size:
1181 1174 861
AhdI GACnn_n'nnGTC
Cuts at: 0 2316 3216
Size: 2316 900
AleI CACnn'nnGTG
Cuts at: 0 874 2260 3216
Size: 874 1386 956
Fragments arranged by size:
1386 956 874
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 163 253 1054 3216
Size: 163 90 801 2162
Fragments arranged by size:
2162 801 163 90
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 2095 2127 3216
Size: 2095 32 1089
Fragments arranged by size:
2095 1089 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1921 2592 3216
Size: 1921 671 624
Fragments arranged by size:
1921 671 624
ApaI G_GGCC'C
Cuts at: 0 684 975 1150 2535 3216
Size: 684 291 175 1385 681
Fragments arranged by size:
1385 684 681 291 175
ApaLI G'TGCA_C
Cuts at: 0 726 733 3216
Size: 726 7 2483
Fragments arranged by size:
2483 726 7
ApoI r'AATT_y
Cuts at: 0 2152 2200 3191 3216
Size: 2152 48 991 25
Fragments arranged by size:
2152 991 48 25
AvaI C'yCGr_G
Cuts at: 0 93 485 958 1383 1796 2295 2687 3146
Size: 93 392 473 425 413 499 392 459
Cuts at: 3146 3216
Size: 70
Fragments arranged by size:
499 473 459 425 413 392 392 93
70
AvrII C'CTAG_G
Cuts at: 0 2838 3216
Size: 2838 378
BamHI G'GATC_C
Cuts at: 0 1388 1878 2139 3216
Size: 1388 490 261 1077
Fragments arranged by size:
1388 1077 490 261
BanI G'GyrC_C
Cuts at: 0 19 186 547 765 804 909 1336 1819
Size: 19 167 361 218 39 105 427 483
Cuts at: 1819 2071 3216
Size: 252 1145
Fragments arranged by size:
1145 483 427 361 252 218 167 105
39 19
BanII G_rGCy'C
Cuts at: 0 494 684 975 1150 1248 1606 1796 1865
Size: 494 190 291 175 98 358 190 69
Cuts at: 1865 2535 3153 3216
Size: 670 618 63
Fragments arranged by size:
670 618 494 358 291 190 190 175
98 69 63
BbeI G_GCGC'C
Cuts at: 0 808 3216
Size: 808 2408
BbsI GAAGACnn'nnnn_
Cuts at: 0 366 885 1874 2899 3216
Size: 366 519 989 1025 317
Fragments arranged by size:
1025 989 519 366 317
BbvCI CC'TCA_GC
Cuts at: 0 1368 2010 2648 3216
Size: 1368 642 638 568
Fragments arranged by size:
1368 642 638 568
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1893 1927 2286 2320 3216
Size: 1893 34 359 34 896
Fragments arranged by size:
1893 896 359 34 34
BclI T'GATC_A
Cuts at: 0 2772 3216
Size: 2772 444
BfrBI ATG'CAT
Cuts at: 0 3202 3216
Size: 3202 14
BglI GCCn_nnn'nGGC
Cuts at: 0 1534 1700 1760 2390 3216
Size: 1534 166 60 630 826
Fragments arranged by size:
1534 826 630 166 60
BglII A'GATC_T
Cuts at: 0 2570 3216
Size: 2570 646
Bme1580I G_kGCm'C
Cuts at: 0 191 429 592 684 730 737 770 975
Size: 191 238 163 92 46 7 33 205
Cuts at: 975 1150 2076 2535 3216
Size: 175 926 459 681
Fragments arranged by size:
926 681 459 238 205 191 175 163
92 46 33 7
BmgBI CAC'GTC
Cuts at: 0 906 3216
Size: 906 2310
BmrI ACTGGGnnnn_n'
Cuts at: 0 117 1208 3216
Size: 117 1091 2008
Fragments arranged by size:
2008 1091 117
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 816 1398 2307 3216
Size: 816 582 909 909
Fragments arranged by size:
909 909 816 582
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 682 1597 2155 2487 3034 3216
Size: 640 42 915 558 332 547 182
Fragments arranged by size:
915 640 558 547 332 182 42
Bpu10I CC'TnA_GC
Cuts at: 0 1368 2010 2648 2870 3216
Size: 1368 642 638 222 346
Fragments arranged by size:
1368 642 638 346 222
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 352 463 3216
Size: 352 111 2753
Fragments arranged by size:
2753 352 111
BsaI GGTCTCn'nnnn_
Cuts at: 0 1003 1714 2115 2303 3216
Size: 1003 711 401 188 913
Fragments arranged by size:
1003 913 711 401 188
BsaAI yAC'GTr
Cuts at: 0 224 2715 3216
Size: 224 2491 501
Fragments arranged by size:
2491 501 224
BsaBI GATnn'nnATC
Cuts at: 0 259 3216
Size: 259 2957
BsaHI Gr'CG_yC
Cuts at: 0 13 173 805 824 1160 3058 3216
Size: 13 160 632 19 336 1898 158
Fragments arranged by size:
1898 632 336 160 158 19 13
BsaWI w'CCGG_w
Cuts at: 0 16 293 1076 2027 2068 2903 3216
Size: 16 277 783 951 41 835 313
Fragments arranged by size:
951 835 783 313 277 41 16
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 103 133 295 325 1145 1175 1213 1243
Size: 103 30 162 30 820 30 38 30
Cuts at: 1243 1659 1689 1834 1864 2095 2125 2491 2521
Size: 416 30 145 30 231 30 366 30
Cuts at: 2521 2715 2745 3216
Size: 194 30 471
Fragments arranged by size:
820 471 416 366 231 194 162 145
103 38 30 30 30 30 30 30
30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 196 271 766 1546 1600 1855 1858 1998
Size: 196 75 495 780 54 255 3 140
Cuts at: 1998 2052 2389 2392 2736 2947 3048 3118 3121
Size: 54 337 3 344 211 101 70 3
Cuts at: 3121 3216
Size: 95
Fragments arranged by size:
780 495 344 337 255 211 196 140
101 95 75 70 54 54 3 3
3
BseYI C'CCAG_C
Cuts at: 0 190 677 974 1493 1678 3216
Size: 190 487 297 519 185 1538
Fragments arranged by size:
1538 519 487 297 190 185
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1189 1577 3216
Size: 598 591 388 1639
Fragments arranged by size:
1639 598 591 388
BsiEI CG_ry'CG
Cuts at: 0 1049 3216
Size: 1049 2167
BsiHKAI G_wGCw'C
Cuts at: 0 494 673 730 737 3216
Size: 494 179 57 7 2479
Fragments arranged by size:
2479 494 179 57 7
BsmI GAATG_Cn'
Cuts at: 0 524 1326 3216
Size: 524 802 1890
Fragments arranged by size:
1890 802 524
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1563 1947 3216
Size: 1563 384 1269
Fragments arranged by size:
1563 1269 384
Bsp1286I G_dGCh'C
Cuts at: 0 191 429 494 592 673 684 730 737
Size: 191 238 65 98 81 11 46 7
Cuts at: 737 770 975 1150 1248 1606 1796 1865 2076
Size: 33 205 175 98 358 190 69 211
Cuts at: 2076 2535 3153 3216
Size: 459 618 63
Fragments arranged by size:
618 459 358 238 211 205 191 190
175 98 98 81 69 65 63 46
33 11 7
BspEI T'CCGG_A
Cuts at: 0 2027 3216
Size: 2027 1189
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 921 1217 1607 1678 1699 1831 2321 2331
Size: 921 296 390 71 21 132 490 10
Cuts at: 2331 3216
Size: 885
Fragments arranged by size:
921 885 490 390 296 132 71 21
10
BsrBI CCG'CTC
Cuts at: 0 1156 2397 2529 2874 3216
Size: 1156 1241 132 345 342
Fragments arranged by size:
1241 1156 345 342 132
BsrDI GCAATG_nn'
Cuts at: 0 2962 3216
Size: 2962 254
BsrFI r'CCGG_y
Cuts at: 0 136 274 739 1194 2078 2896 2985 3216
Size: 136 138 465 455 884 818 89 231
Fragments arranged by size:
884 818 465 455 231 138 136 89
BssHII G'CGCG_C
Cuts at: 0 743 745 3216
Size: 743 2 2471
Fragments arranged by size:
2471 743 2
BssSI C'ACGA_G
Cuts at: 0 1780 3216
Size: 1780 1436
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1973 3216
Size: 1973 1243
BstEII G'GTnAC_C
Cuts at: 0 777 1362 3216
Size: 777 585 1854
Fragments arranged by size:
1854 777 585
BstXI CCAn_nnnn'nTGG
Cuts at: 0 843 1113 2511 2747 3216
Size: 843 270 1398 236 469
Fragments arranged by size:
1398 843 469 270 236
BstYI r'GATC_y
Cuts at: 0 254 1388 1878 2129 2139 2570 3216
Size: 254 1134 490 251 10 431 646
Fragments arranged by size:
1134 646 490 431 254 251 10
BstZ17I GTA'TAC
Cuts at: 0 435 3216
Size: 435 2781
Bsu36I CC'TnA_GG
Cuts at: 0 179 1745 1838 3216
Size: 179 1566 93 1378
Fragments arranged by size:
1566 1378 179 93
BtgI C'CryG_G
Cuts at: 0 23 339 818 842 872 2096 2114 2258
Size: 23 316 479 24 30 1224 18 144
Cuts at: 2258 2540 3216
Size: 282 676
Fragments arranged by size:
1224 676 479 316 282 144 30 24
23 18
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1724 3216
Size: 1724 1492
BtsI GCAGTG_nn'
Cuts at: 0 1704 1971 2490 3216
Size: 1704 267 519 726
Fragments arranged by size:
1704 726 519 267
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 3150 3185 3216
Size: 3150 35 31
Fragments arranged by size:
3150 35 31
EaeI y'GGCC_r
Cuts at: 0 415 719 878 1457 1526 1701 2264 2594
Size: 415 304 159 579 69 175 563 330
Cuts at: 2594 2898 3216
Size: 304 318
Fragments arranged by size:
579 563 415 330 318 304 304 175
159 69
EarI CTCTTCn'nnn_
Cuts at: 0 1933 2070 2696 2713 2884 3216
Size: 1933 137 626 17 171 332
Fragments arranged by size:
1933 626 332 171 137 17
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 187 553 2670 3216
Size: 187 366 2117 546
Fragments arranged by size:
2117 546 366 187
EcoICRI GAG'CTC
Cuts at: 0 492 3216
Size: 492 2724
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 640 682 1597 2155 2356 2487 2911 3034
Size: 640 42 915 558 201 131 424 123
Cuts at: 3034 3216
Size: 182
Fragments arranged by size:
915 640 558 424 201 182 131 123
42
EcoNI CCTnn'n_nnAGG
Cuts at: 0 397 917 2028 2338 3100 3216
Size: 397 520 1111 310 762 116
Fragments arranged by size:
1111 762 520 397 310 116
EcoO109I rG'GnC_Cy
Cuts at: 0 512 681 1146 1413 2375 2467 2532 3216
Size: 512 169 465 267 962 92 65 684
Fragments arranged by size:
962 684 512 465 267 169 92 65
EcoRI G'AATT_C
Cuts at: 0 2152 3216
Size: 2152 1064
HaeII r_GCGC'y
Cuts at: 0 808 842 3216
Size: 808 34 2374
Fragments arranged by size:
2374 808 34
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 295 327 1145 1177 2302 2334 2539 2571
Size: 295 32 818 32 1125 32 205 32
Cuts at: 2571 2574 2606 2715 2747 2788 2820 3082 3114
Size: 3 32 109 32 41 32 262 32
Cuts at: 3114 3216
Size: 102
Fragments arranged by size:
1125 818 295 262 205 109 102 41
32 32 32 32 32 32 32 32
3
HincII GTy'rAC
Cuts at: 0 828 2346 3216
Size: 828 1518 870
Fragments arranged by size:
1518 870 828
KasI G'GCGC_C
Cuts at: 0 804 3216
Size: 804 2412
KpnI G_GTAC'C
Cuts at: 0 913 3216
Size: 913 2303
MfeI C'AATT_G
Cuts at: 0 2756 3216
Size: 2756 460
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2713 3216
Size: 2713 503
MscI TGG'CCA
Cuts at: 0 417 880 1459 3216
Size: 417 463 579 1757
Fragments arranged by size:
1757 579 463 417
MslI CAynn'nnrTG
Cuts at: 0 601 874 1453 1709 2260 2354 2522 3216
Size: 601 273 579 256 551 94 168 694
Fragments arranged by size:
694 601 579 551 273 256 168 94
MspA1I CmG'CkG
Cuts at: 0 25 60 341 644 699 820 1113 1497
Size: 25 35 281 303 55 121 293 384
Cuts at: 1497 1921 2014 3117 3216
Size: 424 93 1103 99
Fragments arranged by size:
1103 424 384 303 293 281 121 99
93 55 35 25
NaeI GCC'GGC
Cuts at: 0 138 741 2080 3216
Size: 138 603 1339 1136
Fragments arranged by size:
1339 1136 603 138
NarI GG'CG_CC
Cuts at: 0 805 3216
Size: 805 2411
NcoI C'CATG_G
Cuts at: 0 2096 2540 3216
Size: 2096 444 676
Fragments arranged by size:
2096 676 444
NdeI CA'TA_TG
Cuts at: 0 1521 3216
Size: 1521 1695
NgoMIV G'CCGG_C
Cuts at: 0 136 739 2078 3216
Size: 136 603 1339 1138
Fragments arranged by size:
1339 1138 603 136
NsiI A_TGCA'T
Cuts at: 0 3204 3216
Size: 3204 12
NspI r_CATG'y
Cuts at: 0 2359 3216
Size: 2359 857
PciI A'CATG_T
Cuts at: 0 2355 3216
Size: 2355 861
PflMI CCAn_nnn'nTGG
Cuts at: 0 2907 3216
Size: 2907 309
PfoI T'CCnGG_A
Cuts at: 0 263 617 1874 2132 3216
Size: 263 354 1257 258 1084
Fragments arranged by size:
1257 1084 354 263 258
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2095 2127 3216
Size: 2095 32 1089
Fragments arranged by size:
2095 1089 32
PshAI GACnn'nnGTC
Cuts at: 0 1015 3216
Size: 1015 2201
PspOMI G'GGCC_C
Cuts at: 0 680 971 1146 2531 3216
Size: 680 291 175 1385 685
Fragments arranged by size:
1385 685 680 291 175
PspXI vC'TCGA_Gb
Cuts at: 0 3146 3216
Size: 3146 70
PstI C_TGCA'G
Cuts at: 0 472 1348 2383 2929 3216
Size: 472 876 1035 546 287
Fragments arranged by size:
1035 876 546 472 287
PvuII CAG'CTG
Cuts at: 0 1113 1497 2014 3216
Size: 1113 384 517 1202
Fragments arranged by size:
1202 1113 517 384
SacI G_AGCT'C
Cuts at: 0 494 3216
Size: 494 2722
SacII CC_GC'GG
Cuts at: 0 26 342 821 3216
Size: 26 316 479 2395
Fragments arranged by size:
2395 479 316 26
SalI G'TCGA_C
Cuts at: 0 826 3216
Size: 826 2390
SapI GCTCTTCn'nnn_
Cuts at: 0 2884 3216
Size: 2884 332
SexAI A'CCwGG_T
Cuts at: 0 2105 3216
Size: 2105 1111
SfcI C'TryA_G
Cuts at: 0 468 609 1059 1344 2379 2925 3216
Size: 468 141 450 285 1035 546 291
Fragments arranged by size:
1035 546 468 450 291 285 141
SfoI GGC'GCC
Cuts at: 0 806 3216
Size: 806 2410
SgrAI Cr'CCGG_yG
Cuts at: 0 739 3216
Size: 739 2477
SmaI CCC'GGG
Cuts at: 0 487 960 1798 3216
Size: 487 473 838 1418
Fragments arranged by size:
1418 838 487 473
SmlI C'TyrA_G
Cuts at: 0 93 367 478 3146 3216
Size: 93 274 111 2668 70
Fragments arranged by size:
2668 274 111 93 70
StuI AGG'CCT
Cuts at: 0 2044 3216
Size: 2044 1172
StyI C'CwwG_G
Cuts at: 0 1409 2096 2540 2838 3216
Size: 1409 687 444 298 378
Fragments arranged by size:
1409 687 444 378 298
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 399 1005 2016 2366 3216
Size: 399 606 1011 350 850
Fragments arranged by size:
1011 850 606 399 350
TatI w'GTAC_w
Cuts at: 0 1316 3216
Size: 1316 1900
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 444 476 1260 1292 1659 1691 1834 1866
Size: 444 32 784 32 367 32 143 32
Cuts at: 1866 1870 1902 2131 2163 3216
Size: 4 32 229 32 1053
Fragments arranged by size:
1053 784 444 367 229 143 32 32
32 32 32 32 4
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 198 600 843 1297 2637 3216
Size: 198 402 243 454 1340 579
Fragments arranged by size:
1340 579 454 402 243 198
XhoI C'TCGA_G
Cuts at: 0 93 3146 3216
Size: 93 3053 70
Fragments arranged by size:
3053 93 70
XmaI C'CCGG_G
Cuts at: 0 485 958 1796 3216
Size: 485 473 838 1420
Fragments arranged by size:
1420 838 485 473
XmnI GAAnn'nnTTC
Cuts at: 0 1852 3216
Size: 1852 1364
ZraI GAC'GTC
Cuts at: 0 14 3216
Size: 14 3202
Enzymes that do cut:
AarI AatII AccI Acc65I AcuI AflIII AhdI AleI
AlfI AloI AlwNI ApaI ApaLI ApoI AvaI AvrII
BamHI BanI BanII BbeI BbsI BbvCI BcgI BclI
BfrBI BglI BglII Bme1580I BmgBI BmrI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BssSI BstAPI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI
CspCI EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I
EcoRI HaeII Hin4I HincII KasI KpnI MfeI MmeI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NsiI NspI PciI PflMI PfoI PpiI PshAI PspOMI
PspXI PstI PvuII SacI SacII SalI SapI SexAI
SfcI SfoI SgrAI SmaI SmlI StuI StyI TaqII
TatI TstI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AclI AfeI AflII AgeI AscI AseI AsiSI BaeI
BciVI BlpI BmtI BsiWI BspHI BsrGI BstBI ClaI
DraI DraIII DrdI EagI EcoRV FalI FseI FspI
FspAI HindIII HpaI MluI NheI NotI NruI PacI
PasI PmeI PmlI PpuMI PsiI PsrI PvuI RsrII
SanDI SbfI ScaI SfiI SgfI SnaBI SpeI SphI
SrfI SspI SwaI Tth111I XbaI