(Linear) (Six-base) MAPSORT of: KIBB3505.seq  Check: 8609  from: 1  to: 1062



KIBB3505.orf

 With 182 enzymes: SgfI * 

                        February 28, 2007 11:24  ..

AccI GT'mk_AC

Cuts at:      0     218    1062 
   Size:        218     844
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     362     725    1062 
   Size:        362     363     337

  Fragments arranged by size: 

                 363     362     337
 
AflIII A'CryG_T

Cuts at:      0     970    1062 
   Size:        970      92
 
AvaI C'yCGr_G

Cuts at:      0     296     428    1062 
   Size:        296     132     634

  Fragments arranged by size: 

                 634     296     132
 
BanII G_rGCy'C

Cuts at:      0     844    1062 
   Size:        844     218
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     929    1062 
   Size:        929     133
 
BfrBI ATG'CAT

Cuts at:      0     729    1062 
   Size:        729     333
 
BglII A'GATC_T

Cuts at:      0     576     613    1062 
   Size:        576      37     449

  Fragments arranged by size: 

                 576     449      37
 
BlpI GC'TnA_GC

Cuts at:      0      13    1062 
   Size:         13    1049
 
BmtI G_CTAG'C

Cuts at:      0     331    1062 
   Size:        331     731
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     392    1062 
   Size:        353      39     670

  Fragments arranged by size: 

                 670     353      39
 
BsaI GGTCTCn'nnnn_

Cuts at:      0    1060    1062 
   Size:       1060       2
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     205    1062 
   Size:        205     857
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1015    1062 
   Size:       1015      47
 
BsiEI CG_ry'CG

Cuts at:      0      60    1062 
   Size:         60    1002
 
BsmI GAATG_Cn'

Cuts at:      0     731    1062 
   Size:        731     331
 
Bsp1286I G_dGCh'C

Cuts at:      0     844    1062 
   Size:        844     218
 
BsrBI CCG'CTC

Cuts at:      0      42     621    1062 
   Size:         42     579     441

  Fragments arranged by size: 

                 579     441      42
 
BsrDI GCAATG_nn'

Cuts at:      0     636    1062 
   Size:        636     426
 
BsrGI T'GTAC_A

Cuts at:      0     405    1062 
   Size:        405     657
 
BssHII G'CGCG_C

Cuts at:      0      91    1062 
   Size:         91     971
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     367    1062 
   Size:        367     695
 
BstBI TT'CG_AA

Cuts at:      0     644    1062 
   Size:        644     418
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     175    1062 
   Size:        175     887
 
BstYI r'GATC_y

Cuts at:      0     424     576     613     813    1062 
   Size:        424     152      37     200     249

  Fragments arranged by size: 

                 424     249     200     152      37
 
BtsI GCAGTG_nn'

Cuts at:      0     218    1062 
   Size:        218     844
 
DraI TTT'AAA

Cuts at:      0     588    1062 
   Size:        588     474
 
DraIII CAC_nnn'GTG

Cuts at:      0     682    1062 
   Size:        682     380
 
EarI CTCTTCn'nnn_

Cuts at:      0     182     884    1062 
   Size:        182     702     178

  Fragments arranged by size: 

                 702     182     178
 
EciI GGCGGAnnnnnnnnn_nn'

Cuts at:      0     316    1062 
   Size:        316     746
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     353     362     392     725    1062 
   Size:        353       9      30     333     337

  Fragments arranged by size: 

                 353     337     333      30       9
 
EcoNI CCTnn'n_nnAGG

Cuts at:      0      70    1062 
   Size:         70     992
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     297     834    1062 
   Size:        297     537     228

  Fragments arranged by size: 

                 537     297     228
 
HaeII r_GCGC'y

Cuts at:      0      20    1062 
   Size:         20    1042
 
MfeI C'AATT_G

Cuts at:      0     143    1062 
   Size:        143     919
 
MspA1I CmG'CkG

Cuts at:      0     898    1062 
   Size:        898     164
 
NdeI CA'TA_TG

Cuts at:      0     757     885    1062 
   Size:        757     128     177

  Fragments arranged by size: 

                 757     177     128
 
NheI G'CTAG_C

Cuts at:      0     327    1062 
   Size:        327     735
 
NsiI A_TGCA'T

Cuts at:      0     731    1062 
   Size:        731     331
 
NspI r_CATG'y

Cuts at:      0     974    1062 
   Size:        974      88
 
PciI A'CATG_T

Cuts at:      0     970    1062 
   Size:        970      92
 
PsiI TTA'TAA

Cuts at:      0     379    1062 
   Size:        379     683
 
PstI C_TGCA'G

Cuts at:      0     363     456    1062 
   Size:        363      93     606

  Fragments arranged by size: 

                 606     363      93
 
PvuII CAG'CTG

Cuts at:      0     898    1062 
   Size:        898     164
 
SexAI A'CCwGG_T

Cuts at:      0     692    1062 
   Size:        692     370
 
SfcI C'TryA_G

Cuts at:      0     219     359     452     542    1062 
   Size:        219     140      93      90     520

  Fragments arranged by size: 

                 520     219     140      93      90
 
SmaI CCC'GGG

Cuts at:      0     298    1062 
   Size:        298     764
 
SrfI GCCC'GGGC

Cuts at:      0     298    1062 
   Size:        298     764
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     976    1062 
   Size:        976      86
 
TatI w'GTAC_w

Cuts at:      0     139     405    1062 
   Size:        139     266     657

  Fragments arranged by size: 

                 657     266     139
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     384     416    1062 
   Size:        384      32     646

  Fragments arranged by size: 

                 646     384      32
 
XmaI C'CCGG_G

Cuts at:      0     296    1062 
   Size:        296     766
 

 Enzymes that do cut:

     AccI     AcuI   AflIII     AvaI    BanII     BbsI    BfrBI    BglII
     BlpI     BmtI     BpmI     BsaI    BseRI     BsgI    BsiEI     BsmI
 Bsp1286I    BsrBI    BsrDI    BsrGI   BssHII   BstAPI    BstBI    BstXI
    BstYI     BtsI     DraI   DraIII     EarI     EciI  Eco57MI    EcoNI
     FalI    HaeII     MfeI   MspA1I     NdeI     NheI     NsiI     NspI
     PciI     PsiI     PstI    PvuII    SexAI     SfcI     SmaI     SrfI
    TaqII     TatI     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
     BcgI    BciVI     BclI     BglI Bme1580I    BmgBI     BmrI     BplI
   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI    BsaWI    BsaXI    BseYI
  BsiHKAI    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrFI    BssSI
   BstEII  BstZ17I   Bsu36I     BtgI    BtgZI     ClaI    CspCI     DrdI
     EaeI     EagI  EcoICRI EcoO109I    EcoRI    EcoRV     FseI     FspI
    FspAI    Hin4I   HincII  HindIII     HpaI     KasI     KpnI     MluI
     MmeI     MscI     MslI     NaeI     NarI     NcoI   NgoMIV     NotI
     NruI     PacI     PasI    PflMI     PfoI     PmeI     PmlI     PpiI
    PpuMI    PshAI   PspOMI    PspXI     PsrI     PvuI    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI     SfiI     SfoI
     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI     SspI     StuI
     StyI     SwaI  Tth111I     XbaI     XcmI     XhoI     XmnI     ZraI