Restriction map for pF1KB3506
 (Linear) (Six-base) MAPSORT of: KIBB3506.seq  Check: 3206  from: 1  to: 807



KIBB3506.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     600     807 
   Size:        600     207
 
AlwNI CAG_nnn'CTG

Cuts at:      0     414     807 
   Size:        414     393
 
AvaI C'yCGr_G

Cuts at:      0     402     807 
   Size:        402     405
 
BanI G'GyrC_C

Cuts at:      0     214     479     807 
   Size:        214     265     328

  Fragments arranged by size: 

                 328     265     214
 
BanII G_rGCy'C

Cuts at:      0     663     807 
   Size:        663     144
 
BbeI G_GCGC'C

Cuts at:      0     218     483     807 
   Size:        218     265     324

  Fragments arranged by size: 

                 324     265     218
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      66     807 
   Size:         66     741
 
BclI T'GATC_A

Cuts at:      0     602     807 
   Size:        602     205
 
BglI GCCn_nnn'nGGC

Cuts at:      0     187     807 
   Size:        187     620
 
BlpI GC'TnA_GC

Cuts at:      0     172     253     498     807 
   Size:        172      81     245     309

  Fragments arranged by size: 

                 309     245     172      81
 
Bme1580I G_kGCm'C

Cuts at:      0     282     807 
   Size:        282     525
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     250     294     368     807 
   Size:        250      44      74     439

  Fragments arranged by size: 

                 439     250      74      44
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     424     768     807 
   Size:         51     373     344      39

  Fragments arranged by size: 

                 373     344      51      39
 
BsaBI GATnn'nnATC

Cuts at:      0     268     807 
   Size:        268     539
 
BsaHI Gr'CG_yC

Cuts at:      0     215     480     807 
   Size:        215     265     327

  Fragments arranged by size: 

                 327     265     215
 
BsaWI w'CCGG_w

Cuts at:      0     473     807 
   Size:        473     334
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     468     498     766     796     807 
   Size:        468      30     268      30      11

  Fragments arranged by size: 

                 468     268      30      30      11
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     339     766     807 
   Size:        339     427      41

  Fragments arranged by size: 

                 427     339      41
 
BsiEI CG_ry'CG

Cuts at:      0     474     807 
   Size:        474     333
 
Bsp1286I G_dGCh'C

Cuts at:      0     282     663     807 
   Size:        282     381     144

  Fragments arranged by size: 

                 381     282     144
 
BsrDI GCAATG_nn'

Cuts at:      0     384     807 
   Size:        384     423
 
BsrGI T'GTAC_A

Cuts at:      0     287     807 
   Size:        287     520
 
BtgI C'CryG_G

Cuts at:      0     596     807 
   Size:        596     211
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     162     279     807 
   Size:        162     117     528

  Fragments arranged by size: 

                 528     162     117
 
BtsI GCAGTG_nn'

Cuts at:      0      44     711     807 
   Size:         44     667      96

  Fragments arranged by size: 

                 667      96      44
 
EaeI y'GGCC_r

Cuts at:      0       2      44      56     372     741     807 
   Size:          2      42      12     316     369      66

  Fragments arranged by size: 

                 369     316      66      42      12       2
 
EarI CTCTTCn'nnn_

Cuts at:      0      31     807 
   Size:         31     776
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      51     424     600     768     807 
   Size:         51     373     176     168      39

  Fragments arranged by size: 

                 373     176     168      51      39
 
EcoO109I rG'GnC_Cy

Cuts at:      0     411     434     807 
   Size:        411      23     373

  Fragments arranged by size: 

                 411     373      23
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0      42     807 
   Size:         42     765
 
HaeII r_GCGC'y

Cuts at:      0     218     483     807 
   Size:        218     265     324

  Fragments arranged by size: 

                 324     265     218
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     766     798     807 
   Size:        766      32       9

  Fragments arranged by size: 

                 766      32       9
 
KasI G'GCGC_C

Cuts at:      0     214     479     807 
   Size:        214     265     328

  Fragments arranged by size: 

                 328     265     214
 
MscI TGG'CCA

Cuts at:      0       4      58     807 
   Size:          4      54     749

  Fragments arranged by size: 

                 749      54       4
 
MspA1I CmG'CkG

Cuts at:      0     212     257     444     598     740     807 
   Size:        212      45     187     154     142      67

  Fragments arranged by size: 

                 212     187     154     142      67      45
 
NarI GG'CG_CC

Cuts at:      0     215     480     807 
   Size:        215     265     327

  Fragments arranged by size: 

                 327     265     215
 
PasI CC'CwG_GG

Cuts at:      0      96     807 
   Size:         96     711
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     306     414     807 
   Size:        306     108     393

  Fragments arranged by size: 

                 393     306     108
 
PfoI T'CCnGG_A

Cuts at:      0     137     807 
   Size:        137     670
 
PpuMI rG'GwC_Cy

Cuts at:      0     434     807 
   Size:        434     373
 
PshAI GACnn'nnGTC

Cuts at:      0     133     807 
   Size:        133     674
 
PvuII CAG'CTG

Cuts at:      0     257     444     807 
   Size:        257     187     363

  Fragments arranged by size: 

                 363     257     187
 
SacII CC_GC'GG

Cuts at:      0     599     807 
   Size:        599     208
 
SanDI GG'GwC_CC

Cuts at:      0     434     807 
   Size:        434     373
 
SapI GCTCTTCn'nnn_

Cuts at:      0      31     807 
   Size:         31     776
 
SfcI C'TryA_G

Cuts at:      0     524     807 
   Size:        524     283
 
SfoI GGC'GCC

Cuts at:      0     216     481     807 
   Size:        216     265     326

  Fragments arranged by size: 

                 326     265     216
 
StyI C'CwwG_G

Cuts at:      0     489     531     807 
   Size:        489      42     276

  Fragments arranged by size: 

                 489     276      42
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     572     627     807 
   Size:        572      55     180

  Fragments arranged by size: 

                 572     180      55
 
TatI w'GTAC_w

Cuts at:      0     287     669     807 
   Size:        287     382     138

  Fragments arranged by size: 

                 382     287     138
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     468     500     807 
   Size:        468      32     307

  Fragments arranged by size: 

                 468     307      32
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     744     807 
   Size:        744      63
 
XmnI GAAnn'nnTTC

Cuts at:      0      25     807 
   Size:         25     782
 

 Enzymes that do cut:

     AcuI    AlwNI     AvaI     BanI    BanII     BbeI     BbsI     BclI
     BglI     BlpI Bme1580I     BmrI     BpmI    BsaBI    BsaHI    BsaWI
    BsaXI    BseRI    BsiEI Bsp1286I    BsrDI    BsrGI     BtgI    BtgZI
     BtsI     EaeI     EarI  Eco57MI EcoO109I     FalI    HaeII    Hin4I
     KasI     MscI   MspA1I     NarI     PasI    PflMI     PfoI    PpuMI
    PshAI    PvuII    SacII    SanDI     SapI     SfcI     SfoI     StyI
    TaqII     TatI     TstI     XcmI     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AfeI    AflII   AflIII
     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BbvCI     BcgI
    BciVI    BfrBI    BglII    BmgBI     BmtI     BplI   Bpu10I    BpuEI
     BsaI    BsaAI    BseYI     BsgI  BsiHKAI    BsiWI     BsmI    BsmBI
    BspEI    BspHI    BspMI    BsrBI    BsrFI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     ClaI    CspCI
     DraI   DraIII     DrdI     EagI     EciI  EcoICRI    EcoNI    EcoRI
    EcoRV     FseI     FspI    FspAI   HincII  HindIII     HpaI     KpnI
     MfeI     MluI     MmeI     MslI     NaeI     NcoI     NdeI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PciI     PmeI
     PmlI     PpiI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI
    RsrII     SacI     SalI     SbfI     ScaI    SexAI     SfiI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI  Tth111I     XbaI     XhoI     XmaI     ZraI
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