(Linear) (Six-base) MAPSORT of: KIBB3506.seq Check: 3206 from: 1 to: 807 KIBB3506.orf With 182 enzymes: SgfI * March 3, 2008 10:56 .. AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 600 807 Size: 600 207 AlwNI CAG_nnn'CTG Cuts at: 0 414 807 Size: 414 393 AvaI C'yCGr_G Cuts at: 0 402 807 Size: 402 405 BanI G'GyrC_C Cuts at: 0 214 479 807 Size: 214 265 328 Fragments arranged by size: 328 265 214 BanII G_rGCy'C Cuts at: 0 663 807 Size: 663 144 BbeI G_GCGC'C Cuts at: 0 218 483 807 Size: 218 265 324 Fragments arranged by size: 324 265 218 BbsI GAAGACnn'nnnn_ Cuts at: 0 66 807 Size: 66 741 BclI T'GATC_A Cuts at: 0 602 807 Size: 602 205 BglI GCCn_nnn'nGGC Cuts at: 0 187 807 Size: 187 620 BlpI GC'TnA_GC Cuts at: 0 172 253 498 807 Size: 172 81 245 309 Fragments arranged by size: 309 245 172 81 Bme1580I G_kGCm'C Cuts at: 0 282 807 Size: 282 525 BmrI ACTGGGnnnn_n' Cuts at: 0 250 294 368 807 Size: 250 44 74 439 Fragments arranged by size: 439 250 74 44 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 424 768 807 Size: 51 373 344 39 Fragments arranged by size: 373 344 51 39 BsaBI GATnn'nnATC Cuts at: 0 268 807 Size: 268 539 BsaHI Gr'CG_yC Cuts at: 0 215 480 807 Size: 215 265 327 Fragments arranged by size: 327 265 215 BsaWI w'CCGG_w Cuts at: 0 473 807 Size: 473 334 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 468 498 766 796 807 Size: 468 30 268 30 11 Fragments arranged by size: 468 268 30 30 11 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 339 766 807 Size: 339 427 41 Fragments arranged by size: 427 339 41 BsiEI CG_ry'CG Cuts at: 0 474 807 Size: 474 333 Bsp1286I G_dGCh'C Cuts at: 0 282 663 807 Size: 282 381 144 Fragments arranged by size: 381 282 144 BsrDI GCAATG_nn' Cuts at: 0 384 807 Size: 384 423 BsrGI T'GTAC_A Cuts at: 0 287 807 Size: 287 520 BtgI C'CryG_G Cuts at: 0 596 807 Size: 596 211 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 162 279 807 Size: 162 117 528 Fragments arranged by size: 528 162 117 BtsI GCAGTG_nn' Cuts at: 0 44 711 807 Size: 44 667 96 Fragments arranged by size: 667 96 44 EaeI y'GGCC_r Cuts at: 0 2 44 56 372 741 807 Size: 2 42 12 316 369 66 Fragments arranged by size: 369 316 66 42 12 2 EarI CTCTTCn'nnn_ Cuts at: 0 31 807 Size: 31 776 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 51 424 600 768 807 Size: 51 373 176 168 39 Fragments arranged by size: 373 176 168 51 39 EcoO109I rG'GnC_Cy Cuts at: 0 411 434 807 Size: 411 23 373 Fragments arranged by size: 411 373 23 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 42 807 Size: 42 765 HaeII r_GCGC'y Cuts at: 0 218 483 807 Size: 218 265 324 Fragments arranged by size: 324 265 218 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 766 798 807 Size: 766 32 9 Fragments arranged by size: 766 32 9 KasI G'GCGC_C Cuts at: 0 214 479 807 Size: 214 265 328 Fragments arranged by size: 328 265 214 MscI TGG'CCA Cuts at: 0 4 58 807 Size: 4 54 749 Fragments arranged by size: 749 54 4 MspA1I CmG'CkG Cuts at: 0 212 257 444 598 740 807 Size: 212 45 187 154 142 67 Fragments arranged by size: 212 187 154 142 67 45 NarI GG'CG_CC Cuts at: 0 215 480 807 Size: 215 265 327 Fragments arranged by size: 327 265 215 PasI CC'CwG_GG Cuts at: 0 96 807 Size: 96 711 PflMI CCAn_nnn'nTGG Cuts at: 0 306 414 807 Size: 306 108 393 Fragments arranged by size: 393 306 108 PfoI T'CCnGG_A Cuts at: 0 137 807 Size: 137 670 PpuMI rG'GwC_Cy Cuts at: 0 434 807 Size: 434 373 PshAI GACnn'nnGTC Cuts at: 0 133 807 Size: 133 674 PvuII CAG'CTG Cuts at: 0 257 444 807 Size: 257 187 363 Fragments arranged by size: 363 257 187 SacII CC_GC'GG Cuts at: 0 599 807 Size: 599 208 SanDI GG'GwC_CC Cuts at: 0 434 807 Size: 434 373 SapI GCTCTTCn'nnn_ Cuts at: 0 31 807 Size: 31 776 SfcI C'TryA_G Cuts at: 0 524 807 Size: 524 283 SfoI GGC'GCC Cuts at: 0 216 481 807 Size: 216 265 326 Fragments arranged by size: 326 265 216 StyI C'CwwG_G Cuts at: 0 489 531 807 Size: 489 42 276 Fragments arranged by size: 489 276 42 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 572 627 807 Size: 572 55 180 Fragments arranged by size: 572 180 55 TatI w'GTAC_w Cuts at: 0 287 669 807 Size: 287 382 138 Fragments arranged by size: 382 287 138 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 0 468 500 807 Size: 468 32 307 Fragments arranged by size: 468 307 32 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 744 807 Size: 744 63 XmnI GAAnn'nnTTC Cuts at: 0 25 807 Size: 25 782 Enzymes that do cut: AcuI AlwNI AvaI BanI BanII BbeI BbsI BclI BglI BlpI Bme1580I BmrI BpmI BsaBI BsaHI BsaWI BsaXI BseRI BsiEI Bsp1286I BsrDI BsrGI BtgI BtgZI BtsI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I KasI MscI MspA1I NarI PasI PflMI PfoI PpuMI PshAI PvuII SacII SanDI SapI SfcI SfoI StyI TaqII TatI TstI XcmI XmnI Enzymes that do not cut: AarI AatII AccI Acc65I AclI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII BaeI BamHI BbvCI BcgI BciVI BfrBI BglII BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI BseYI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrFI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HincII HindIII HpaI KpnI MfeI MluI MmeI MslI NaeI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PciI PmeI PmlI PpiI PsiI PspOMI PspXI PsrI PstI PvuI RsrII SacI SalI SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI XmaI ZraI