Restriction map for
pF1KB3508
(Linear) (Six-base) MAPSORT of: KIBB3508.seq Check: 1935 from: 1 to: 984
KIBB3508.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 840 891 937 984
Size: 840 51 46 47
Fragments arranged by size:
840 51 47 46
AflIII A'CryG_T
Cuts at: 0 309 984
Size: 309 675
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 113 145 984
Size: 113 32 839
Fragments arranged by size:
839 113 32
AlwNI CAG_nnn'CTG
Cuts at: 0 514 984
Size: 514 470
ApaI G_GGCC'C
Cuts at: 0 362 984
Size: 362 622
ApoI r'AATT_y
Cuts at: 0 69 984
Size: 69 915
BanII G_rGCy'C
Cuts at: 0 362 945 984
Size: 362 583 39
Fragments arranged by size:
583 362 39
BbsI GAAGACnn'nnnn_
Cuts at: 0 312 984
Size: 312 672
BciVI GTATCCnnnnn_n'
Cuts at: 0 324 984
Size: 324 660
BfrBI ATG'CAT
Cuts at: 0 640 792 984
Size: 640 152 192
Fragments arranged by size:
640 192 152
BglII A'GATC_T
Cuts at: 0 830 984
Size: 830 154
BlpI GC'TnA_GC
Cuts at: 0 808 984
Size: 808 176
Bme1580I G_kGCm'C
Cuts at: 0 362 984
Size: 362 622
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 403 984
Size: 374 29 581
Fragments arranged by size:
581 374 29
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 964 984
Size: 964 20
BsaI GGTCTCn'nnnn_
Cuts at: 0 208 676 984
Size: 208 468 308
Fragments arranged by size:
468 308 208
BsaAI yAC'GTr
Cuts at: 0 312 984
Size: 312 672
BsaBI GATnn'nnATC
Cuts at: 0 718 984
Size: 718 266
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 400 427 967 984
Size: 400 27 540 17
Fragments arranged by size:
540 400 27 17
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 487 984
Size: 487 497
BsiHKAI G_wGCw'C
Cuts at: 0 338 434 984
Size: 338 96 550
Fragments arranged by size:
550 338 96
BsmI GAATG_Cn'
Cuts at: 0 599 792 984
Size: 599 193 192
Fragments arranged by size:
599 193 192
Bsp1286I G_dGCh'C
Cuts at: 0 338 362 434 945 984
Size: 338 24 72 511 39
Fragments arranged by size:
511 338 72 39 24
BsrFI r'CCGG_y
Cuts at: 0 377 984
Size: 377 607
BsrGI T'GTAC_A
Cuts at: 0 293 984
Size: 293 691
BstAPI GCAn_nnn'nTGC
Cuts at: 0 798 984
Size: 798 186
BstYI r'GATC_y
Cuts at: 0 389 830 984
Size: 389 441 154
Fragments arranged by size:
441 389 154
EarI CTCTTCn'nnn_
Cuts at: 0 257 984
Size: 257 727
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 374 403 840 891 937 984
Size: 374 29 437 51 46 47
Fragments arranged by size:
437 374 51 47 46 29
EcoNI CCTnn'n_nnAGG
Cuts at: 0 328 984
Size: 328 656
EcoO109I rG'GnC_Cy
Cuts at: 0 358 984
Size: 358 626
EcoRI G'AATT_C
Cuts at: 0 69 984
Size: 69 915
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 354 984
Size: 354 630
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 596 628 984
Size: 596 32 356
Fragments arranged by size:
596 356 32
HincII GTy'rAC
Cuts at: 0 207 345 984
Size: 207 138 639
Fragments arranged by size:
639 207 138
HindIII A'AGCT_T
Cuts at: 0 123 949 984
Size: 123 826 35
Fragments arranged by size:
826 123 35
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 407 417 727 984
Size: 407 10 310 257
Fragments arranged by size:
407 310 257 10
MspA1I CmG'CkG
Cuts at: 0 884 984
Size: 884 100
NsiI A_TGCA'T
Cuts at: 0 642 794 984
Size: 642 152 190
Fragments arranged by size:
642 190 152
PflMI CCAn_nnn'nTGG
Cuts at: 0 381 984
Size: 381 603
PsiI TTA'TAA
Cuts at: 0 556 984
Size: 556 428
PspOMI G'GGCC_C
Cuts at: 0 358 984
Size: 358 626
SfcI C'TryA_G
Cuts at: 0 649 984
Size: 649 335
SmlI C'TyrA_G
Cuts at: 0 979 984
Size: 979 5
StuI AGG'CCT
Cuts at: 0 451 861 984
Size: 451 410 123
Fragments arranged by size:
451 410 123
StyI C'CwwG_G
Cuts at: 0 420 984
Size: 420 564
TatI w'GTAC_w
Cuts at: 0 293 984
Size: 293 691
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 65 97 267 299 984
Size: 65 32 170 32 685
Fragments arranged by size:
685 170 65 32 32
Enzymes that do cut:
AcuI AflIII AloI AlwNI ApaI ApoI BanII BbsI
BciVI BfrBI BglII BlpI Bme1580I BpmI BpuEI BsaI
BsaAI BsaBI BseRI BsgI BsiHKAI BsmI Bsp1286I BsrFI
BsrGI BstAPI BstYI EarI Eco57MI EcoNI EcoO109I EcoRI
FalI Hin4I HincII HindIII MmeI MspA1I NsiI PflMI
PsiI PspOMI SfcI SmlI StuI StyI TatI TstI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AleI AlfI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BclI
BglI BmgBI BmrI BmtI BplI Bpu10I BsaHI BsaWI
BsaXI BseYI BsiEI BsiWI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII
DrdI EaeI EagI EciI EcoICRI EcoRV FseI FspI
FspAI HaeII HpaI KasI KpnI MfeI MluI MscI
MslI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NspI PacI PasI PciI PfoI PmeI PmlI
PpiI PpuMI PshAI PspXI PsrI PstI PvuI PvuII
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TaqII Tth111I XbaI XcmI
XhoI XmaI XmnI ZraI