Restriction map for
pF1KB3511
(Linear) (Six-base) MAPSORT of: KIBB3511.seq Check: 726 from: 1 to: 1401
KIBB3511.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AatII G_ACGT'C
Cuts at: 0 890 1401
Size: 890 511
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1327 1401
Size: 1327 74
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1393 1401
Size: 1393 8
AlwNI CAG_nnn'CTG
Cuts at: 0 391 835 864 1401
Size: 391 444 29 537
Fragments arranged by size:
537 444 391 29
ApoI r'AATT_y
Cuts at: 0 274 823 1401
Size: 274 549 578
Fragments arranged by size:
578 549 274
BamHI G'GATC_C
Cuts at: 0 379 1401
Size: 379 1022
BanI G'GyrC_C
Cuts at: 0 295 306 519 612 1401
Size: 295 11 213 93 789
Fragments arranged by size:
789 295 213 93 11
BanII G_rGCy'C
Cuts at: 0 77 364 794 1401
Size: 77 287 430 607
Fragments arranged by size:
607 430 287 77
BbeI G_GCGC'C
Cuts at: 0 523 1401
Size: 523 878
BbvCI CC'TCA_GC
Cuts at: 0 350 1401
Size: 350 1051
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1048 1082 1401
Size: 1048 34 319
Fragments arranged by size:
1048 319 34
BglII A'GATC_T
Cuts at: 0 1109 1265 1401
Size: 1109 156 136
Fragments arranged by size:
1109 156 136
BlpI GC'TnA_GC
Cuts at: 0 1301 1401
Size: 1301 100
Bme1580I G_kGCm'C
Cuts at: 0 311 984 1401
Size: 311 673 417
Fragments arranged by size:
673 417 311
BmrI ACTGGGnnnn_n'
Cuts at: 0 313 1401
Size: 313 1088
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 752 1401
Size: 752 649
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 623 832 931 1401
Size: 613 10 209 99 470
Fragments arranged by size:
613 470 209 99 10
Bpu10I CC'TnA_GC
Cuts at: 0 350 836 1054 1401
Size: 350 486 218 347
Fragments arranged by size:
486 350 347 218
BsaHI Gr'CG_yC
Cuts at: 0 520 887 1401
Size: 520 367 514
Fragments arranged by size:
520 514 367
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 556 586 1401
Size: 556 30 815
Fragments arranged by size:
815 556 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 802 1401
Size: 802 599
BseYI C'CCAG_C
Cuts at: 0 225 242 1366 1401
Size: 225 17 1124 35
Fragments arranged by size:
1124 225 35 17
BsiHKAI G_wGCw'C
Cuts at: 0 258 794 1401
Size: 258 536 607
Fragments arranged by size:
607 536 258
Bsp1286I G_dGCh'C
Cuts at: 0 77 258 311 364 794 984 1401
Size: 77 181 53 53 430 190 417
Fragments arranged by size:
430 417 190 181 77 53 53
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 807 1401
Size: 807 594
BsrDI GCAATG_nn'
Cuts at: 0 1064 1401
Size: 1064 337
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1353 1401
Size: 1353 48
BstXI CCAn_nnnn'nTGG
Cuts at: 0 233 647 1401
Size: 233 414 754
Fragments arranged by size:
754 414 233
BstYI r'GATC_y
Cuts at: 0 379 1109 1265 1401
Size: 379 730 156 136
Fragments arranged by size:
730 379 156 136
Bsu36I CC'TnA_GG
Cuts at: 0 737 1401
Size: 737 664
BtgI C'CryG_G
Cuts at: 0 515 696 1401
Size: 515 181 705
Fragments arranged by size:
705 515 181
BtsI GCAGTG_nn'
Cuts at: 0 549 575 1257 1401
Size: 549 26 682 144
Fragments arranged by size:
682 549 144 26
EaeI y'GGCC_r
Cuts at: 0 1135 1401
Size: 1135 266
EarI CTCTTCn'nnn_
Cuts at: 0 146 757 1401
Size: 146 611 644
Fragments arranged by size:
644 611 146
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1246 1401
Size: 1246 155
EcoICRI GAG'CTC
Cuts at: 0 792 1401
Size: 792 609
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 613 623 832 931 1327 1401
Size: 613 10 209 99 396 74
Fragments arranged by size:
613 396 209 99 74 10
EcoNI CCTnn'n_nnAGG
Cuts at: 0 814 1401
Size: 814 587
EcoO109I rG'GnC_Cy
Cuts at: 0 762 769 832 1401
Size: 762 7 63 569
Fragments arranged by size:
762 569 63 7
EcoRI G'AATT_C
Cuts at: 0 274 1401
Size: 274 1127
HaeII r_GCGC'y
Cuts at: 0 523 1401
Size: 523 878
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 642 674 890 922 1401
Size: 642 32 216 32 479
Fragments arranged by size:
642 479 216 32 32
KasI G'GCGC_C
Cuts at: 0 519 1401
Size: 519 882
MfeI C'AATT_G
Cuts at: 0 537 1401
Size: 537 864
MscI TGG'CCA
Cuts at: 0 1137 1401
Size: 1137 264
MslI CAynn'nnrTG
Cuts at: 0 724 1401
Size: 724 677
MspA1I CmG'CkG
Cuts at: 0 246 920 1305 1314 1401
Size: 246 674 385 9 87
Fragments arranged by size:
674 385 246 87 9
NarI GG'CG_CC
Cuts at: 0 520 1401
Size: 520 881
NcoI C'CATG_G
Cuts at: 0 515 1401
Size: 515 886
NspI r_CATG'y
Cuts at: 0 127 1374 1401
Size: 127 1247 27
Fragments arranged by size:
1247 127 27
PasI CC'CwG_GG
Cuts at: 0 70 357 398 1401
Size: 70 287 41 1003
Fragments arranged by size:
1003 287 70 41
PfoI T'CCnGG_A
Cuts at: 0 476 908 1401
Size: 476 432 493
Fragments arranged by size:
493 476 432
PpuMI rG'GwC_Cy
Cuts at: 0 762 769 832 1401
Size: 762 7 63 569
Fragments arranged by size:
762 569 63 7
PshAI GACnn'nnGTC
Cuts at: 0 767 1401
Size: 767 634
PstI C_TGCA'G
Cuts at: 0 559 1265 1401
Size: 559 706 136
Fragments arranged by size:
706 559 136
PvuII CAG'CTG
Cuts at: 0 246 1305 1314 1401
Size: 246 1059 9 87
Fragments arranged by size:
1059 246 87 9
SacI G_AGCT'C
Cuts at: 0 794 1401
Size: 794 607
SanDI GG'GwC_CC
Cuts at: 0 769 1401
Size: 769 632
SfcI C'TryA_G
Cuts at: 0 182 555 630 1261 1401
Size: 182 373 75 631 140
Fragments arranged by size:
631 373 182 140 75
SfoI GGC'GCC
Cuts at: 0 521 1401
Size: 521 880
SphI G_CATG'C
Cuts at: 0 1374 1401
Size: 1374 27
StuI AGG'CCT
Cuts at: 0 175 1401
Size: 175 1226
StyI C'CwwG_G
Cuts at: 0 299 515 1401
Size: 299 216 886
Fragments arranged by size:
886 299 216
TatI w'GTAC_w
Cuts at: 0 962 1401
Size: 962 439
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 556 588 1401
Size: 556 32 813
Fragments arranged by size:
813 556 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 55 1401
Size: 55 1346
XmnI GAAnn'nnTTC
Cuts at: 0 865 1401
Size: 865 536
ZraI GAC'GTC
Cuts at: 0 888 1401
Size: 888 513
Enzymes that do cut:
AatII AcuI AlfI AlwNI ApoI BamHI BanI BanII
BbeI BbvCI BcgI BglII BlpI Bme1580I BmrI BplI
BpmI Bpu10I BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I
BspMI BsrDI BstAPI BstXI BstYI Bsu36I BtgI BtsI
EaeI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI
HaeII Hin4I KasI MfeI MscI MslI MspA1I NarI
NcoI NspI PasI PfoI PpuMI PshAI PstI PvuII
SacI SanDI SfcI SfoI SphI StuI StyI TatI
TstI XcmI XmnI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AseI AsiSI
AvaI AvrII BaeI BbsI BciVI BclI BfrBI BglI
BmgBI BmtI BpuEI BsaI BsaAI BsaBI BsaWI BsgI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI
BsrGI BssHII BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DraIII DrdI EagI EcoRV FalI FseI
FspI FspAI HincII HindIII HpaI KpnI MluI MmeI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PciI PflMI PmeI PmlI PpiI PsiI PspOMI PspXI
PsrI PvuI RsrII SacII SalI SapI SbfI ScaI
SexAI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI
SrfI SspI SwaI TaqII Tth111I XbaI XhoI XmaI