Restriction map for
pF1KB3539
(Linear) (Six-base) MAPSORT of: KIBB3539.seq Check: 3711 from: 1 to: 1032
KIBB3539.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
Acc65I G'GTAC_C
Cuts at: 0 591 1032
Size: 591 441
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 857 1032
Size: 509 348 175
Fragments arranged by size:
509 348 175
AlwNI CAG_nnn'CTG
Cuts at: 0 456 1032
Size: 456 576
ApoI r'AATT_y
Cuts at: 0 34 818 1032
Size: 34 784 214
Fragments arranged by size:
784 214 34
AvaI C'yCGr_G
Cuts at: 0 595 1032
Size: 595 437
BanI G'GyrC_C
Cuts at: 0 106 591 1032
Size: 106 485 441
Fragments arranged by size:
485 441 106
BanII G_rGCy'C
Cuts at: 0 806 1032
Size: 806 226
BbsI GAAGACnn'nnnn_
Cuts at: 0 782 852 1032
Size: 782 70 180
Fragments arranged by size:
782 180 70
BglII A'GATC_T
Cuts at: 0 282 1032
Size: 282 750
BmrI ACTGGGnnnn_n'
Cuts at: 0 674 768 1032
Size: 674 94 264
Fragments arranged by size:
674 264 94
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 532 1032
Size: 532 500
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 954 1032
Size: 954 78
Bpu10I CC'TnA_GC
Cuts at: 0 712 1032
Size: 712 320
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 529 1032
Size: 529 503
BsaWI w'CCGG_w
Cuts at: 0 389 1032
Size: 389 643
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 640 1032
Size: 640 392
BsiHKAI G_wGCw'C
Cuts at: 0 806 1032
Size: 806 226
Bsp1286I G_dGCh'C
Cuts at: 0 806 1032
Size: 806 226
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 425 1032
Size: 425 607
BsrDI GCAATG_nn'
Cuts at: 0 888 1032
Size: 888 144
BsrFI r'CCGG_y
Cuts at: 0 332 1032
Size: 332 700
BssSI C'ACGA_G
Cuts at: 0 926 1032
Size: 926 106
BstXI CCAn_nnnn'nTGG
Cuts at: 0 642 1032
Size: 642 390
BstYI r'GATC_y
Cuts at: 0 282 1032
Size: 282 750
Bsu36I CC'TnA_GG
Cuts at: 0 344 1032
Size: 344 688
DraIII CAC_nnn'GTG
Cuts at: 0 582 921 1032
Size: 582 339 111
Fragments arranged by size:
582 339 111
EarI CTCTTCn'nnn_
Cuts at: 0 290 1032
Size: 290 742
EcoICRI GAG'CTC
Cuts at: 0 804 1032
Size: 804 228
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 509 857 954 1032
Size: 509 348 97 78
Fragments arranged by size:
509 348 97 78
HincII GTy'rAC
Cuts at: 0 56 1032
Size: 56 976
HindIII A'AGCT_T
Cuts at: 0 356 1032
Size: 356 676
KpnI G_GTAC'C
Cuts at: 0 595 1032
Size: 595 437
MslI CAynn'nnrTG
Cuts at: 0 1009 1032
Size: 1009 23
MspA1I CmG'CkG
Cuts at: 0 456 1032
Size: 456 576
PflMI CCAn_nnn'nTGG
Cuts at: 0 582 1032
Size: 582 450
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 942 974 1032
Size: 942 32 58
Fragments arranged by size:
942 58 32
PsiI TTA'TAA
Cuts at: 0 965 1032
Size: 965 67
PstI C_TGCA'G
Cuts at: 0 446 1032
Size: 446 586
PvuII CAG'CTG
Cuts at: 0 456 1032
Size: 456 576
SacI G_AGCT'C
Cuts at: 0 806 1032
Size: 806 226
SexAI A'CCwGG_T
Cuts at: 0 587 1032
Size: 587 445
SfcI C'TryA_G
Cuts at: 0 442 1032
Size: 442 590
SmlI C'TyrA_G
Cuts at: 0 544 1032
Size: 544 488
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 68 94 1032
Size: 68 26 938
Fragments arranged by size:
938 68 26
XbaI T'CTAG_A
Cuts at: 0 534 1032
Size: 534 498
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 642 1032
Size: 642 390
XmnI GAAnn'nnTTC
Cuts at: 0 742 1032
Size: 742 290
Enzymes that do cut:
Acc65I AcuI AlwNI ApoI AvaI BanI BanII BbsI
BglII BmrI BplI BpmI Bpu10I BpuEI BsaWI BseRI
BsiHKAI Bsp1286I BspMI BsrDI BsrFI BssSI BstXI BstYI
Bsu36I DraIII EarI EcoICRI Eco57MI HincII HindIII KpnI
MslI MspA1I PflMI PpiI PsiI PstI PvuII SacI
SexAI SfcI SmlI TaqII XbaI XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AflIII AgeI
AhdI AleI AlfI AloI ApaI ApaLI AscI AseI
AsiSI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BfrBI BglI BlpI Bme1580I BmgBI BmtI BsaI
BsaAI BsaBI BsaHI BsaXI BseYI BsgI BsiEI BsiWI
BsmI BsmBI BspEI BspHI BsrBI BsrGI BssHII BstAPI
BstBI BstEII BstZ17I BtgI BtgZI BtsI ClaI CspCI
DraI DrdI EaeI EagI EciI EcoNI EcoO109I EcoRI
EcoRV FalI FseI FspI FspAI HaeII Hin4I HpaI
KasI MfeI MluI MmeI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI NspI PacI
PasI PciI PfoI PmeI PmlI PpuMI PshAI PspOMI
PspXI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI
SpeI SphI SrfI SspI StuI StyI SwaI TatI
TstI Tth111I XhoI XmaI ZraI