(Linear) (Six-base) MAPSORT of: KIBB3544.seq  Check: 412  from: 1  to: 324



KIBB3544.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

ApaLI G'TGCA_C

Cuts at:      0      73     324 
   Size:         73     251
 
ApoI r'AATT_y

Cuts at:      0     106     324 
   Size:        106     218
 
AvaI C'yCGr_G

Cuts at:      0     119     324 
   Size:        119     205
 
BanI G'GyrC_C

Cuts at:      0     250     260     324 
   Size:        250      10      64

  Fragments arranged by size: 

                 250      64      10
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     288     324 
   Size:        288      36
 
Bme1580I G_kGCm'C

Cuts at:      0      77     263     324 
   Size:         77     186      61

  Fragments arranged by size: 

                 186      77      61
 
BmrI ACTGGGnnnn_n'

Cuts at:      0     266     324 
   Size:        266      58
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     324 
   Size:        218     106
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0      26     216     246     324 
   Size:         26     190      30      78

  Fragments arranged by size: 

                 190      78      30      26
 
BseYI C'CCAG_C

Cuts at:      0     176     324 
   Size:        176     148
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      28     324 
   Size:         28     296
 
BsiHKAI G_wGCw'C

Cuts at:      0      77     324 
   Size:         77     247
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      26     324 
   Size:         26     298
 
Bsp1286I G_dGCh'C

Cuts at:      0      77     263     324 
   Size:         77     186      61

  Fragments arranged by size: 

                 186      77      61
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      73     324 
   Size:         73     251
 
EaeI y'GGCC_r

Cuts at:      0      57     324 
   Size:         57     267
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     218     324 
   Size:        218     106
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     192     216     224     248     324 
   Size:        192      24       8      24      76

  Fragments arranged by size: 

                 192      76      24      24       8
 
MslI CAynn'nnrTG

Cuts at:      0      85     324 
   Size:         85     239
 
NspI r_CATG'y

Cuts at:      0     286     324 
   Size:        286      38
 
SmaI CCC'GGG

Cuts at:      0     121     324 
   Size:        121     203
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     278     324 
   Size:        278      46
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      28     324 
   Size:         28     296
 
XmaI C'CCGG_G

Cuts at:      0     119     324 
   Size:        119     205
 

 Enzymes that do cut:

    ApaLI     ApoI     AvaI     BanI     BbsI Bme1580I     BmrI     BpmI
    BsaXI    BseYI     BsgI  BsiHKAI    BsmBI Bsp1286I    BspMI     EaeI
  Eco57MI    Hin4I     MslI     NspI     SmaI    TaqII     TstI     XmaI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
     AscI     AseI    AsiSI    AvrII     BaeI    BamHI    BanII     BbeI
    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI    BglII     BlpI
    BmgBI     BmtI     BplI   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI
    BsaHI    BsaWI    BseRI    BsiEI    BsiWI     BsmI    BspEI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI
   BstEII    BstXI    BstYI  BstZ17I   Bsu36I     BtgI    BtgZI     BtsI
     ClaI    CspCI     DraI   DraIII     DrdI     EagI     EarI     EciI
  EcoICRI    EcoNI EcoO109I    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI     MmeI     MscI   MspA1I     NaeI     NarI     NcoI     NdeI
   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI    PpuMI    PshAI     PsiI
   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI
    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI     SfcI
     SfiI     SfoI     SgfI    SgrAI     SmlI    SnaBI     SpeI     SphI
     SrfI     SspI     StuI     StyI     SwaI     TatI  Tth111I     XbaI
     XcmI     XhoI     XmnI     ZraI