Restriction map for
pF1KB3557
(Linear) (Six-base) MAPSORT of: KIBB3557.seq Check: 2958 from: 1 to: 900
KIBB3557.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 440 900
Size: 43 397 460
Fragments arranged by size:
460 397 43
AflII C'TTAA_G
Cuts at: 0 870 900
Size: 870 30
AflIII A'CryG_T
Cuts at: 0 281 900
Size: 281 619
AleI CACnn'nnGTG
Cuts at: 0 685 900
Size: 685 215
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 703 900
Size: 703 197
ApoI r'AATT_y
Cuts at: 0 29 900
Size: 29 871
BanII G_rGCy'C
Cuts at: 0 545 900
Size: 545 355
BbsI GAAGACnn'nnnn_
Cuts at: 0 13 206 900
Size: 13 193 694
Fragments arranged by size:
694 193 13
BclI T'GATC_A
Cuts at: 0 318 900
Size: 318 582
BglII A'GATC_T
Cuts at: 0 618 900
Size: 618 282
BmgBI CAC'GTC
Cuts at: 0 220 900
Size: 220 680
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 414 900
Size: 414 486
Bpu10I CC'TnA_GC
Cuts at: 0 845 900
Size: 845 55
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 772 900
Size: 772 128
BsaI GGTCTCn'nnnn_
Cuts at: 0 487 900
Size: 487 413
BsaAI yAC'GTr
Cuts at: 0 282 832 900
Size: 282 550 68
Fragments arranged by size:
550 282 68
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 709 900
Size: 709 191
Bsp1286I G_dGCh'C
Cuts at: 0 545 900
Size: 545 355
BsrGI T'GTAC_A
Cuts at: 0 741 900
Size: 741 159
BstYI r'GATC_y
Cuts at: 0 618 900
Size: 618 282
BtsI GCAGTG_nn'
Cuts at: 0 338 900
Size: 338 562
ClaI AT'CG_AT
Cuts at: 0 188 900
Size: 188 712
DraI TTT'AAA
Cuts at: 0 518 900
Size: 518 382
DraIII CAC_nnn'GTG
Cuts at: 0 835 900
Size: 835 65
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 43 414 440 900
Size: 43 371 26 460
Fragments arranged by size:
460 371 43 26
EcoRI G'AATT_C
Cuts at: 0 29 900
Size: 29 871
HindIII A'AGCT_T
Cuts at: 0 337 900
Size: 337 563
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 240 900
Size: 240 660
MslI CAynn'nnrTG
Cuts at: 0 326 685 900
Size: 326 359 215
Fragments arranged by size:
359 326 215
PflMI CCAn_nnn'nTGG
Cuts at: 0 357 900
Size: 357 543
PmlI CAC'GTG
Cuts at: 0 832 900
Size: 832 68
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 619 651 900
Size: 619 32 249
Fragments arranged by size:
619 249 32
PstI C_TGCA'G
Cuts at: 0 449 900
Size: 449 451
SexAI A'CCwGG_T
Cuts at: 0 66 900
Size: 66 834
SfcI C'TryA_G
Cuts at: 0 136 445 900
Size: 136 309 455
Fragments arranged by size:
455 309 136
SmlI C'TyrA_G
Cuts at: 0 11 751 870 900
Size: 11 740 119 30
Fragments arranged by size:
740 119 30 11
StuI AGG'CCT
Cuts at: 0 380 672 900
Size: 380 292 228
Fragments arranged by size:
380 292 228
StyI C'CwwG_G
Cuts at: 0 291 900
Size: 291 609
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 338 697 900
Size: 338 359 203
Fragments arranged by size:
359 338 203
TatI w'GTAC_w
Cuts at: 0 741 900
Size: 741 159
XbaI T'CTAG_A
Cuts at: 0 36 900
Size: 36 864
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 244 900
Size: 244 656
Enzymes that do cut:
AcuI AflII AflIII AleI AlfI ApoI BanII BbsI
BclI BglII BmgBI BpmI Bpu10I BpuEI BsaI BsaAI
BsgI Bsp1286I BsrGI BstYI BtsI ClaI DraI DraIII
Eco57MI EcoRI HindIII MmeI MslI PflMI PmlI PpiI
PstI SexAI SfcI SmlI StuI StyI TaqII TatI
XbaI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AgeI AhdI
AloI AlwNI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BanI BbeI BbvCI BcgI BciVI
BfrBI BglI BlpI Bme1580I BmrI BmtI BplI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI BsiWI
BsmI BsmBI BspEI BspHI BspMI BsrBI BsrDI BsrFI
BssHII BssSI BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgI BtgZI CspCI DrdI EaeI EagI EarI EciI
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspI FspAI
HaeII Hin4I HincII HpaI KasI KpnI MfeI MluI
MscI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PpuMI PshAI PsiI PspOMI PspXI PsrI PvuI
PvuII RsrII SacI SacII SalI SanDI SapI SbfI
ScaI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI SspI SwaI TstI Tth111I XhoI XmaI
XmnI ZraI