Restriction map for
pF1KB3558
(Linear) (Six-base) MAPSORT of: KIBB3558.seq Check: 764 from: 1 to: 999
KIBB3558.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 0 73 999
Size: 73 926
AccI GT'mk_AC
Cuts at: 0 73 999
Size: 73 926
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 822 999
Size: 822 177
AfeI AGC'GCT
Cuts at: 0 324 490 999
Size: 324 166 509
Fragments arranged by size:
509 324 166
AhdI GACnn_n'nnGTC
Cuts at: 0 723 999
Size: 723 276
AleI CACnn'nnGTG
Cuts at: 0 619 999
Size: 619 380
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 702 917 999
Size: 702 215 82
Fragments arranged by size:
702 215 82
AlwNI CAG_nnn'CTG
Cuts at: 0 630 760 885 999
Size: 630 130 125 114
Fragments arranged by size:
630 130 125 114
ApaI G_GGCC'C
Cuts at: 0 227 999
Size: 227 772
ApaLI G'TGCA_C
Cuts at: 0 763 999
Size: 763 236
AvaI C'yCGr_G
Cuts at: 0 955 999
Size: 955 44
BanI G'GyrC_C
Cuts at: 0 583 999
Size: 583 416
BanII G_rGCy'C
Cuts at: 0 164 227 362 999
Size: 164 63 135 637
Fragments arranged by size:
637 164 135 63
BbsI GAAGACnn'nnnn_
Cuts at: 0 412 999
Size: 412 587
BclI T'GATC_A
Cuts at: 0 593 999
Size: 593 406
BglI GCCn_nnn'nGGC
Cuts at: 0 856 999
Size: 856 143
BglII A'GATC_T
Cuts at: 0 524 999
Size: 524 475
Bme1580I G_kGCm'C
Cuts at: 0 227 588 767 906 999
Size: 227 361 179 139 93
Fragments arranged by size:
361 227 179 139 93
BmgBI CAC'GTC
Cuts at: 0 621 999
Size: 621 378
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 699 999
Size: 699 300
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 652 895 907 966 999
Size: 505 147 243 12 59 33
Fragments arranged by size:
505 243 147 59 33 12
Bpu10I CC'TnA_GC
Cuts at: 0 138 295 999
Size: 138 157 704
Fragments arranged by size:
704 157 138
BsaI GGTCTCn'nnnn_
Cuts at: 0 920 952 999
Size: 920 32 47
Fragments arranged by size:
920 47 32
BsaAI yAC'GTr
Cuts at: 0 399 999
Size: 399 600
BsaHI Gr'CG_yC
Cuts at: 0 70 999
Size: 70 929
BseYI C'CCAG_C
Cuts at: 0 164 502 538 999
Size: 164 338 36 461
Fragments arranged by size:
461 338 164 36
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 643 999
Size: 643 356
BsiEI CG_ry'CG
Cuts at: 0 243 999
Size: 243 756
BsiHKAI G_wGCw'C
Cuts at: 0 305 767 999
Size: 305 462 232
Fragments arranged by size:
462 305 232
BsmI GAATG_Cn'
Cuts at: 0 832 859 999
Size: 832 27 140
Fragments arranged by size:
832 140 27
Bsp1286I G_dGCh'C
Cuts at: 0 164 227 305 362 588 767 906 999
Size: 164 63 78 57 226 179 139 93
Fragments arranged by size:
226 179 164 139 93 78 63 57
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 83 306 999
Size: 83 223 693
Fragments arranged by size:
693 223 83
BsrDI GCAATG_nn'
Cuts at: 0 927 999
Size: 927 72
BsrFI r'CCGG_y
Cuts at: 0 10 999
Size: 10 989
BssSI C'ACGA_G
Cuts at: 0 700 999
Size: 700 299
BstAPI GCAn_nnn'nTGC
Cuts at: 0 873 999
Size: 873 126
BstXI CCAn_nnnn'nTGG
Cuts at: 0 378 471 999
Size: 378 93 528
Fragments arranged by size:
528 378 93
BstYI r'GATC_y
Cuts at: 0 524 999
Size: 524 475
BtgI C'CryG_G
Cuts at: 0 116 309 999
Size: 116 193 690
Fragments arranged by size:
690 193 116
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 164 234 546 999
Size: 164 70 312 453
Fragments arranged by size:
453 312 164 70
BtsI GCAGTG_nn'
Cuts at: 0 652 906 999
Size: 652 254 93
Fragments arranged by size:
652 254 93
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 409 444 999
Size: 409 35 555
Fragments arranged by size:
555 409 35
EaeI y'GGCC_r
Cuts at: 0 770 806 999
Size: 770 36 193
Fragments arranged by size:
770 193 36
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 158 941 999
Size: 158 783 58
Fragments arranged by size:
783 158 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 505 652 822 895 907 966 999
Size: 505 147 170 73 12 59 33
Fragments arranged by size:
505 170 147 73 59 33 12
EcoO109I rG'GnC_Cy
Cuts at: 0 95 203 223 224 717 882 977 999
Size: 95 108 20 1 493 165 95 22
Fragments arranged by size:
493 165 108 95 95 22 20 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 852 999
Size: 852 147
HaeII r_GCGC'y
Cuts at: 0 326 492 999
Size: 326 166 507
Fragments arranged by size:
507 326 166
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 709 741 781 813 917 949 999
Size: 709 32 40 32 104 32 50
Fragments arranged by size:
709 104 50 40 32 32 32
HincII GTy'rAC
Cuts at: 0 74 999
Size: 74 925
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 73 632 999
Size: 73 559 367
Fragments arranged by size:
559 367 73
MscI TGG'CCA
Cuts at: 0 772 999
Size: 772 227
MslI CAynn'nnrTG
Cuts at: 0 469 619 999
Size: 469 150 380
Fragments arranged by size:
469 380 150
MspA1I CmG'CkG
Cuts at: 0 40 133 538 999
Size: 40 93 405 461
Fragments arranged by size:
461 405 93 40
NcoI C'CATG_G
Cuts at: 0 116 999
Size: 116 883
NspI r_CATG'y
Cuts at: 0 740 999
Size: 740 259
PflMI CCAn_nnn'nTGG
Cuts at: 0 647 999
Size: 647 352
PpuMI rG'GwC_Cy
Cuts at: 0 95 203 717 882 999
Size: 95 108 514 165 117
Fragments arranged by size:
514 165 117 108 95
PspOMI G'GGCC_C
Cuts at: 0 223 999
Size: 223 776
PstI C_TGCA'G
Cuts at: 0 881 999
Size: 881 118
PvuII CAG'CTG
Cuts at: 0 538 999
Size: 538 461
SalI G'TCGA_C
Cuts at: 0 72 999
Size: 72 927
ScaI AGT'ACT
Cuts at: 0 793 999
Size: 793 206
SfcI C'TryA_G
Cuts at: 0 513 877 999
Size: 513 364 122
Fragments arranged by size:
513 364 122
SgrAI Cr'CCGG_yG
Cuts at: 0 10 999
Size: 10 989
StyI C'CwwG_G
Cuts at: 0 116 371 999
Size: 116 255 628
Fragments arranged by size:
628 255 116
TatI w'GTAC_w
Cuts at: 0 791 999
Size: 791 208
Tth111I GACn'n_nGTC
Cuts at: 0 244 999
Size: 244 755
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 378 468 648 919 999
Size: 378 90 180 271 80
Fragments arranged by size:
378 271 180 90 80
XmnI GAAnn'nnTTC
Cuts at: 0 832 999
Size: 832 167
ZraI GAC'GTC
Cuts at: 0 71 999
Size: 71 928
Enzymes that do cut:
AatII AccI AcuI AfeI AhdI AleI AlfI AlwNI
ApaI ApaLI AvaI BanI BanII BbsI BclI BglI
BglII Bme1580I BmgBI BplI BpmI Bpu10I BsaI BsaAI
BsaHI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI
BsrDI BsrFI BssSI BstAPI BstXI BstYI BtgI BtgZI
BtsI CspCI EaeI EciI Eco57MI EcoO109I FalI HaeII
Hin4I HincII MmeI MscI MslI MspA1I NcoI NspI
PflMI PpuMI PspOMI PstI PvuII SalI ScaI SfcI
SgrAI StyI TatI Tth111I XcmI XmnI ZraI
Enzymes that do not cut:
AarI Acc65I AclI AflII AflIII AgeI AloI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbvCI
BcgI BciVI BfrBI BlpI BmrI BmtI BpuEI BsaBI
BsaWI BsaXI BseRI BsiWI BsmBI BspEI BspHI BsrBI
BsrGI BssHII BstBI BstEII BstZ17I Bsu36I ClaI DraI
DraIII DrdI EagI EarI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HindIII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PfoI PmeI PmlI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SacI SacII
SanDI SapI SbfI SexAI SfiI SfoI SgfI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI SwaI
TaqII TstI XbaI XhoI XmaI