Restriction map for
pF1KB3561
(Linear) (Six-base) MAPSORT of: KIBB3561.seq Check: 3266 from: 1 to: 621
KIBB3561.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AccI GT'mk_AC
Cuts at: 0 122 449 621
Size: 122 327 172
Fragments arranged by size:
327 172 122
AclI AA'CG_TT
Cuts at: 0 515 621
Size: 515 106
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 621
Size: 493 128
AflIII A'CryG_T
Cuts at: 0 437 621
Size: 437 184
AgeI A'CCGG_T
Cuts at: 0 9 621
Size: 9 612
AlwNI CAG_nnn'CTG
Cuts at: 0 201 621
Size: 201 420
AvaI C'yCGr_G
Cuts at: 0 55 621
Size: 55 566
BanI G'GyrC_C
Cuts at: 0 6 621
Size: 6 615
BanII G_rGCy'C
Cuts at: 0 134 238 422 621
Size: 134 104 184 199
Fragments arranged by size:
199 184 134 104
BbvCI CC'TCA_GC
Cuts at: 0 106 621
Size: 106 515
BclI T'GATC_A
Cuts at: 0 362 621
Size: 362 259
BglI GCCn_nnn'nGGC
Cuts at: 0 353 621
Size: 353 268
BmgBI CAC'GTC
Cuts at: 0 351 621
Size: 351 270
BmrI ACTGGGnnnn_n'
Cuts at: 0 533 621
Size: 533 88
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 621
Size: 560 61
Bpu10I CC'TnA_GC
Cuts at: 0 106 621
Size: 106 515
BsaAI yAC'GTr
Cuts at: 0 438 621
Size: 438 183
BsaWI w'CCGG_w
Cuts at: 0 9 621
Size: 9 612
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 412 621
Size: 412 209
BseYI C'CCAG_C
Cuts at: 0 226 508 621
Size: 226 282 113
Fragments arranged by size:
282 226 113
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 160 621
Size: 160 461
BsiHKAI G_wGCw'C
Cuts at: 0 238 557 621
Size: 238 319 64
Fragments arranged by size:
319 238 64
Bsp1286I G_dGCh'C
Cuts at: 0 134 238 422 557 621
Size: 134 104 184 135 64
Fragments arranged by size:
184 135 134 104 64
BsrFI r'CCGG_y
Cuts at: 0 9 621
Size: 9 612
BstEII G'GTnAC_C
Cuts at: 0 12 621
Size: 12 609
BstYI r'GATC_y
Cuts at: 0 68 621
Size: 68 553
BtgI C'CryG_G
Cuts at: 0 503 596 621
Size: 503 93 25
Fragments arranged by size:
503 93 25
DraIII CAC_nnn'GTG
Cuts at: 0 438 621
Size: 438 183
EaeI y'GGCC_r
Cuts at: 0 500 621
Size: 500 121
EcoICRI GAG'CTC
Cuts at: 0 236 621
Size: 236 385
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 493 560 621
Size: 493 67 61
Fragments arranged by size:
493 67 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 79 621
Size: 79 542
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 32 64 369 401 621
Size: 32 32 305 32 220
Fragments arranged by size:
305 220 32 32 32
HincII GTy'rAC
Cuts at: 0 123 621
Size: 123 498
MscI TGG'CCA
Cuts at: 0 502 621
Size: 502 119
MspA1I CmG'CkG
Cuts at: 0 315 621
Size: 315 306
NcoI C'CATG_G
Cuts at: 0 596 621
Size: 596 25
PflMI CCAn_nnn'nTGG
Cuts at: 0 255 597 621
Size: 255 342 24
Fragments arranged by size:
342 255 24
PstI C_TGCA'G
Cuts at: 0 206 621
Size: 206 415
SacI G_AGCT'C
Cuts at: 0 238 621
Size: 238 383
SalI G'TCGA_C
Cuts at: 0 121 621
Size: 121 500
SfcI C'TryA_G
Cuts at: 0 202 621
Size: 202 419
SmaI CCC'GGG
Cuts at: 0 57 621
Size: 57 564
StyI C'CwwG_G
Cuts at: 0 596 621
Size: 596 25
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 218 621
Size: 218 403
XmaI C'CCGG_G
Cuts at: 0 55 621
Size: 55 566
Enzymes that do cut:
AccI AclI AcuI AflIII AgeI AlwNI AvaI BanI
BanII BbvCI BclI BglI BmgBI BmrI BpmI Bpu10I
BsaAI BsaWI BseRI BseYI BsgI BsiHKAI Bsp1286I BsrFI
BstEII BstYI BtgI DraIII EaeI EcoICRI Eco57MI EcoNI
Hin4I HincII MscI MspA1I NcoI PflMI PstI SacI
SalI SfcI SmaI StyI TaqII XmaI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AhdI AleI AlfI
AloI ApaI ApaLI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbsI BcgI BciVI BfrBI BglII
BlpI Bme1580I BmtI BplI BpuEI BsaI BsaBI BsaHI
BsaXI BsiEI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrBI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI
BstZ17I Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI
EagI EarI EciI EcoO109I EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI MslI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PfoI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI PvuII RsrII SacII SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SphI SrfI SspI StuI SwaI TatI TstI
Tth111I XbaI XcmI XhoI XmnI ZraI