Restriction map for
pF1KB3567
(Linear) (Six-base) MAPSORT of: KIBB3567.seq Check: 4197 from: 1 to: 2142
KIBB3567.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 831 2142
Size: 831 1311
AccI GT'mk_AC
Cuts at: 0 1334 2009 2142
Size: 1334 675 133
Fragments arranged by size:
1334 675 133
Acc65I G'GTAC_C
Cuts at: 0 189 2142
Size: 189 1953
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1635 1737 2142
Size: 1635 102 405
Fragments arranged by size:
1635 405 102
AhdI GACnn_n'nnGTC
Cuts at: 0 1239 2142
Size: 1239 903
AleI CACnn'nnGTG
Cuts at: 0 22 37 2142
Size: 22 15 2105
Fragments arranged by size:
2105 22 15
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1273 2142
Size: 1273 869
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 982 1014 2142
Size: 982 32 1128
Fragments arranged by size:
1128 982 32
AlwNI CAG_nnn'CTG
Cuts at: 0 201 2142
Size: 201 1941
ApaLI G'TGCA_C
Cuts at: 0 532 2142
Size: 532 1610
AvaI C'yCGr_G
Cuts at: 0 1409 1471 2142
Size: 1409 62 671
Fragments arranged by size:
1409 671 62
BanI G'GyrC_C
Cuts at: 0 189 1117 1488 2142
Size: 189 928 371 654
Fragments arranged by size:
928 654 371 189
BanII G_rGCy'C
Cuts at: 0 736 1508 1973 1997 2142
Size: 736 772 465 24 145
Fragments arranged by size:
772 736 465 145 24
BglI GCCn_nnn'nGGC
Cuts at: 0 1301 2142
Size: 1301 841
BglII A'GATC_T
Cuts at: 0 1046 2082 2142
Size: 1046 1036 60
Fragments arranged by size:
1046 1036 60
Bme1580I G_kGCm'C
Cuts at: 0 536 1493 2142
Size: 536 957 649
Fragments arranged by size:
957 649 536
BmgBI CAC'GTC
Cuts at: 0 483 2142
Size: 483 1659
BmrI ACTGGGnnnn_n'
Cuts at: 0 44 279 343 686 2142
Size: 44 235 64 343 1456
Fragments arranged by size:
1456 343 235 64 44
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 750 2142
Size: 750 1392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 745 747 939 1063 2142
Size: 579 166 2 192 124 1079
Fragments arranged by size:
1079 579 192 166 124 2
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 592 700 1057 2142
Size: 592 108 357 1085
Fragments arranged by size:
1085 592 357 108
BsaI GGTCTCn'nnnn_
Cuts at: 0 2041 2142
Size: 2041 101
BsaHI Gr'CG_yC
Cuts at: 0 1067 2142
Size: 1067 1075
BsaWI w'CCGG_w
Cuts at: 0 875 1347 1850 1871 2142
Size: 875 472 503 21 271
Fragments arranged by size:
875 503 472 271 21
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 22 921 1636 2142
Size: 22 899 715 506
Fragments arranged by size:
899 715 506 22
BseYI C'CCAG_C
Cuts at: 0 75 741 1161 1555 1812 2142
Size: 75 666 420 394 257 330
Fragments arranged by size:
666 420 394 330 257 75
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 15 73 376 2142
Size: 15 58 303 1766
Fragments arranged by size:
1766 303 58 15
BsiEI CG_ry'CG
Cuts at: 0 1305 1370 2009 2142
Size: 1305 65 639 133
Fragments arranged by size:
1305 639 133 65
BsiHKAI G_wGCw'C
Cuts at: 0 333 536 1138 1262 1613 1973 2142
Size: 333 203 602 124 351 360 169
Fragments arranged by size:
602 360 351 333 203 169 124
BsmI GAATG_Cn'
Cuts at: 0 102 2142
Size: 102 2040
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1201 1412 2142
Size: 1201 211 730
Fragments arranged by size:
1201 730 211
Bsp1286I G_dGCh'C
Cuts at: 0 333 536 736 1138 1262 1493 1508 1613
Size: 333 203 200 402 124 231 15 105
Cuts at: 1613 1973 1997 2142
Size: 360 24 145
Fragments arranged by size:
402 360 333 231 203 200 145 124
105 24 15
BspEI T'CCGG_A
Cuts at: 0 875 1347 1850 1871 2142
Size: 875 472 503 21 271
Fragments arranged by size:
875 503 472 271 21
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 506 831 1445 1745 2142
Size: 506 325 614 300 397
Fragments arranged by size:
614 506 397 325 300
BsrBI CCG'CTC
Cuts at: 0 729 2142
Size: 729 1413
BsrDI GCAATG_nn'
Cuts at: 0 359 1809 2142
Size: 359 1450 333
Fragments arranged by size:
1450 359 333
BsrFI r'CCGG_y
Cuts at: 0 21 436 1365 2142
Size: 21 415 929 777
Fragments arranged by size:
929 777 415 21
BssHII G'CGCG_C
Cuts at: 0 1414 2142
Size: 1414 728
BstEII G'GTnAC_C
Cuts at: 0 660 2142
Size: 660 1482
BstXI CCAn_nnnn'nTGG
Cuts at: 0 75 2142
Size: 75 2067
BstYI r'GATC_y
Cuts at: 0 1046 1193 1580 2082 2142
Size: 1046 147 387 502 60
Fragments arranged by size:
1046 502 387 147 60
BtgI C'CryG_G
Cuts at: 0 263 1139 2142
Size: 263 876 1003
Fragments arranged by size:
1003 876 263
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 156 2142
Size: 156 1986
DraIII CAC_nnn'GTG
Cuts at: 0 399 2142
Size: 399 1743
DrdI GACnn_nn'nnGTC
Cuts at: 0 2005 2142
Size: 2005 137
EaeI y'GGCC_r
Cuts at: 0 356 411 1302 1367 1397 1925 2142
Size: 356 55 891 65 30 528 217
Fragments arranged by size:
891 528 356 217 65 55 30
EagI C'GGCC_G
Cuts at: 0 1302 1367 2142
Size: 1302 65 775
Fragments arranged by size:
1302 775 65
EarI CTCTTCn'nnn_
Cuts at: 0 169 1543 1613 1654 2142
Size: 169 1374 70 41 488
Fragments arranged by size:
1374 488 169 70 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1450 2142
Size: 1450 692
EcoICRI GAG'CTC
Cuts at: 0 1971 2142
Size: 1971 171
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 579 745 747 939 1063 1635 1737 2142
Size: 579 166 2 192 124 572 102 405
Fragments arranged by size:
579 572 405 192 166 124 102 2
EcoNI CCTnn'n_nnAGG
Cuts at: 0 115 1441 2142
Size: 115 1326 701
Fragments arranged by size:
1326 701 115
EcoO109I rG'GnC_Cy
Cuts at: 0 158 213 220 609 1341 1644 2142
Size: 158 55 7 389 732 303 498
Fragments arranged by size:
732 498 389 303 158 55 7
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1404 1665 2142
Size: 1404 261 477
Fragments arranged by size:
1404 477 261
FspI TGC'GCA
Cuts at: 0 682 2142
Size: 682 1460
HaeII r_GCGC'y
Cuts at: 0 566 724 1296 2142
Size: 566 158 572 846
Fragments arranged by size:
846 572 566 158
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 598 630 916 948 1225 1257 2142
Size: 598 32 286 32 277 32 885
Fragments arranged by size:
885 598 286 277 32 32 32
HincII GTy'rAC
Cuts at: 0 2010 2142
Size: 2010 132
KpnI G_GTAC'C
Cuts at: 0 193 2142
Size: 193 1949
MscI TGG'CCA
Cuts at: 0 1399 1927 2142
Size: 1399 528 215
Fragments arranged by size:
1399 528 215
MslI CAynn'nnrTG
Cuts at: 0 22 37 95 2142
Size: 22 15 58 2047
Fragments arranged by size:
2047 58 22 15
MspA1I CmG'CkG
Cuts at: 0 63 132 456 1141 1659 2127 2142
Size: 63 69 324 685 518 468 15
Fragments arranged by size:
685 518 468 324 69 63 15
NaeI GCC'GGC
Cuts at: 0 438 1367 2142
Size: 438 929 775
Fragments arranged by size:
929 775 438
NdeI CA'TA_TG
Cuts at: 0 957 2142
Size: 957 1185
NgoMIV G'CCGG_C
Cuts at: 0 436 1365 2142
Size: 436 929 777
Fragments arranged by size:
929 777 436
PasI CC'CwG_GG
Cuts at: 0 890 2142
Size: 890 1252
PflMI CCAn_nnn'nTGG
Cuts at: 0 201 1172 2142
Size: 201 971 970
Fragments arranged by size:
971 970 201
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2038 2070 2142
Size: 2038 32 72
Fragments arranged by size:
2038 72 32
PpuMI rG'GwC_Cy
Cuts at: 0 158 213 220 1341 2142
Size: 158 55 7 1121 801
Fragments arranged by size:
1121 801 158 55 7
PshAI GACnn'nnGTC
Cuts at: 0 218 2142
Size: 218 1924
PstI C_TGCA'G
Cuts at: 0 152 1252 2142
Size: 152 1100 890
Fragments arranged by size:
1100 890 152
PvuII CAG'CTG
Cuts at: 0 132 456 1659 2127 2142
Size: 132 324 1203 468 15
Fragments arranged by size:
1203 468 324 132 15
SacI G_AGCT'C
Cuts at: 0 1973 2142
Size: 1973 169
SacII CC_GC'GG
Cuts at: 0 1142 2142
Size: 1142 1000
SalI G'TCGA_C
Cuts at: 0 2008 2142
Size: 2008 134
SanDI GG'GwC_CC
Cuts at: 0 158 220 2142
Size: 158 62 1922
Fragments arranged by size:
1922 158 62
SfcI C'TryA_G
Cuts at: 0 148 1248 2142
Size: 148 1100 894
Fragments arranged by size:
1100 894 148
SgrAI Cr'CCGG_yG
Cuts at: 0 21 2142
Size: 21 2121
SmaI CCC'GGG
Cuts at: 0 1473 2142
Size: 1473 669
SmlI C'TyrA_G
Cuts at: 0 571 715 1072 2142
Size: 571 144 357 1070
Fragments arranged by size:
1070 571 357 144
StuI AGG'CCT
Cuts at: 0 178 580 2142
Size: 178 402 1562
Fragments arranged by size:
1562 402 178
StyI C'CwwG_G
Cuts at: 0 587 1745 2142
Size: 587 1158 397
Fragments arranged by size:
1158 587 397
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 465 1164 2142
Size: 465 699 978
Fragments arranged by size:
978 699 465
TatI w'GTAC_w
Cuts at: 0 26 2142
Size: 26 2116
Tth111I GACn'n_nGTC
Cuts at: 0 484 2142
Size: 484 1658
XbaI T'CTAG_A
Cuts at: 0 771 2142
Size: 771 1371
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 108 1169 2142
Size: 108 1061 973
Fragments arranged by size:
1061 973 108
XmaI C'CCGG_G
Cuts at: 0 1471 2142
Size: 1471 671
Enzymes that do cut:
AarI AccI Acc65I AcuI AhdI AleI AlfI AloI
AlwNI ApaLI AvaI BanI BanII BglI BglII Bme1580I
BmgBI BmrI BplI BpmI BpuEI BsaI BsaHI BsaWI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspMI BsrBI BsrDI BsrFI BssHII BstEII BstXI
BstYI BtgI BtgZI DraIII DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII KpnI MscI MslI MspA1I NaeI NdeI
NgoMIV PasI PflMI PpiI PpuMI PshAI PstI PvuII
SacI SacII SalI SanDI SfcI SgrAI SmaI SmlI
StuI StyI TaqII TatI Tth111I XbaI XcmI XmaI
Enzymes that do not cut:
AatII AclI AfeI AflII AflIII AgeI ApaI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BbsI
BbvCI BcgI BciVI BclI BfrBI BlpI BmtI Bpu10I
BsaAI BsaBI BsaXI BsiWI BspHI BsrGI BssSI BstAPI
BstBI BstZ17I Bsu36I BtsI ClaI CspCI DraI EcoRI
EcoRV FseI FspAI HindIII HpaI KasI MfeI MluI
MmeI NarI NcoI NheI NotI NruI NsiI NspI
PacI PciI PfoI PmeI PmlI PsiI PspOMI PspXI
PsrI PvuI RsrII SapI SbfI ScaI SexAI SfiI
SfoI SgfI SnaBI SpeI SphI SrfI SspI SwaI
TstI XhoI XmnI ZraI