Restriction map for
pF1KB3573
(Linear) (Six-base) MAPSORT of: KIBB3573.seq Check: 9781 from: 1 to: 1608
KIBB3573.orf
With 182 enzymes: SgfI *
October 7, 2009 14:15 ..
AccI GT'mk_AC
Cuts at: 0 1310 1328 1608
Size: 1310 18 280
Fragments arranged by size:
1310 280 18
Acc65I G'GTAC_C
Cuts at: 0 220 477 1052 1608
Size: 220 257 575 556
Fragments arranged by size:
575 556 257 220
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 210 467 540 1368 1608
Size: 76 134 257 73 828 240
Fragments arranged by size:
828 257 240 134 76 73
AflIII A'CryG_T
Cuts at: 0 1343 1608
Size: 1343 265
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 325 1608
Size: 325 1283
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 323 355 1608
Size: 323 32 1253
Fragments arranged by size:
1253 323 32
AlwNI CAG_nnn'CTG
Cuts at: 0 379 650 861 1608
Size: 379 271 211 747
Fragments arranged by size:
747 379 271 211
ApaI G_GGCC'C
Cuts at: 0 281 1608
Size: 281 1327
ApoI r'AATT_y
Cuts at: 0 420 1608
Size: 420 1188
AvrII C'CTAG_G
Cuts at: 0 906 1086 1608
Size: 906 180 522
Fragments arranged by size:
906 522 180
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1036 1069 1608
Size: 1036 33 539
Fragments arranged by size:
1036 539 33
BamHI G'GATC_C
Cuts at: 0 1536 1608
Size: 1536 72
BanI G'GyrC_C
Cuts at: 0 115 220 477 1052 1608
Size: 115 105 257 575 556
Fragments arranged by size:
575 556 257 115 105
BanII G_rGCy'C
Cuts at: 0 281 609 1150 1408 1608
Size: 281 328 541 258 200
Fragments arranged by size:
541 328 281 258 200
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1462 1496 1608
Size: 1462 34 112
Fragments arranged by size:
1462 112 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 838 1608
Size: 838 770
BfrBI ATG'CAT
Cuts at: 0 1467 1608
Size: 1467 141
BglI GCCn_nnn'nGGC
Cuts at: 0 1135 1608
Size: 1135 473
BlpI GC'TnA_GC
Cuts at: 0 779 1608
Size: 779 829
Bme1580I G_kGCm'C
Cuts at: 0 120 281 304 1608
Size: 120 161 23 1304
Fragments arranged by size:
1304 161 120 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 996 1608
Size: 996 612
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1581 1608
Size: 1581 27
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1055 1576 1608
Size: 1055 521 32
Fragments arranged by size:
1055 521 32
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 568 655 691 1608
Size: 568 87 36 917
Fragments arranged by size:
917 568 87 36
BsaHI Gr'CG_yC
Cuts at: 0 1599 1608
Size: 1599 9
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 172 202 1608
Size: 172 30 1406
Fragments arranged by size:
1406 172 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1497 1608
Size: 1497 111
BseYI C'CCAG_C
Cuts at: 0 620 829 1608
Size: 620 209 779
Fragments arranged by size:
779 620 209
BsiHKAI G_wGCw'C
Cuts at: 0 785 1608
Size: 785 823
Bsp1286I G_dGCh'C
Cuts at: 0 120 281 304 609 785 1150 1408 1608
Size: 120 161 23 305 176 365 258 200
Fragments arranged by size:
365 305 258 200 176 161 120 23
BsrBI CCG'CTC
Cuts at: 0 685 1505 1608
Size: 685 820 103
Fragments arranged by size:
820 685 103
BsrDI GCAATG_nn'
Cuts at: 0 177 893 1037 1608
Size: 177 716 144 571
Fragments arranged by size:
716 571 177 144
BsrGI T'GTAC_A
Cuts at: 0 1044 1608
Size: 1044 564
BstAPI GCAn_nnn'nTGC
Cuts at: 0 115 1170 1608
Size: 115 1055 438
Fragments arranged by size:
1055 438 115
BstYI r'GATC_y
Cuts at: 0 1536 1608
Size: 1536 72
Bsu36I CC'TnA_GG
Cuts at: 0 1400 1608
Size: 1400 208
BtgI C'CryG_G
Cuts at: 0 482 1608
Size: 482 1126
BtsI GCAGTG_nn'
Cuts at: 0 104 255 809 1460 1608
Size: 104 151 554 651 148
Fragments arranged by size:
651 554 151 148 104
DraIII CAC_nnn'GTG
Cuts at: 0 250 1608
Size: 250 1358
EaeI y'GGCC_r
Cuts at: 0 485 1377 1608
Size: 485 892 231
Fragments arranged by size:
892 485 231
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 934 1608
Size: 934 674
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 210 467 540 1055 1368 1576 1608
Size: 76 134 257 73 515 313 208 32
Fragments arranged by size:
515 313 257 208 134 76 73 32
EcoNI CCTnn'n_nnAGG
Cuts at: 0 565 1608
Size: 565 1043
EcoO109I rG'GnC_Cy
Cuts at: 0 277 910 1285 1608
Size: 277 633 375 323
Fragments arranged by size:
633 375 323 277
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 452 484 1608
Size: 452 32 1124
Fragments arranged by size:
1124 452 32
HincII GTy'rAC
Cuts at: 0 1311 1608
Size: 1311 297
HindIII A'AGCT_T
Cuts at: 0 138 1608
Size: 138 1470
KpnI G_GTAC'C
Cuts at: 0 224 481 1056 1608
Size: 224 257 575 552
Fragments arranged by size:
575 552 257 224
MfeI C'AATT_G
Cuts at: 0 1290 1608
Size: 1290 318
MscI TGG'CCA
Cuts at: 0 487 1379 1608
Size: 487 892 229
Fragments arranged by size:
892 487 229
MslI CAynn'nnrTG
Cuts at: 0 938 1472 1608
Size: 938 534 136
Fragments arranged by size:
938 534 136
MspA1I CmG'CkG
Cuts at: 0 491 811 1608
Size: 491 320 797
Fragments arranged by size:
797 491 320
NcoI C'CATG_G
Cuts at: 0 482 1608
Size: 482 1126
NsiI A_TGCA'T
Cuts at: 0 1469 1608
Size: 1469 139
PflMI CCAn_nnn'nTGG
Cuts at: 0 650 861 1157 1416 1608
Size: 650 211 296 259 192
Fragments arranged by size:
650 296 259 211 192
PfoI T'CCnGG_A
Cuts at: 0 764 1553 1608
Size: 764 789 55
Fragments arranged by size:
789 764 55
PpuMI rG'GwC_Cy
Cuts at: 0 910 1285 1608
Size: 910 375 323
Fragments arranged by size:
910 375 323
PshAI GACnn'nnGTC
Cuts at: 0 908 1608
Size: 908 700
PspOMI G'GGCC_C
Cuts at: 0 277 1608
Size: 277 1331
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 1608
Size: 1216 32 360
Fragments arranged by size:
1216 360 32
PstI C_TGCA'G
Cuts at: 0 326 614 1608
Size: 326 288 994
Fragments arranged by size:
994 326 288
PvuII CAG'CTG
Cuts at: 0 491 811 1608
Size: 491 320 797
Fragments arranged by size:
797 491 320
SalI G'TCGA_C
Cuts at: 0 1309 1608
Size: 1309 299
ScaI AGT'ACT
Cuts at: 0 930 1608
Size: 930 678
SfcI C'TryA_G
Cuts at: 0 147 322 610 1608
Size: 147 175 288 998
Fragments arranged by size:
998 288 175 147
SmlI C'TyrA_G
Cuts at: 0 583 670 706 1608
Size: 583 87 36 902
Fragments arranged by size:
902 583 87 36
StuI AGG'CCT
Cuts at: 0 308 1138 1608
Size: 308 830 470
Fragments arranged by size:
830 470 308
StyI C'CwwG_G
Cuts at: 0 216 482 906 1086 1337 1374 1608
Size: 216 266 424 180 251 37 234
Fragments arranged by size:
424 266 251 234 216 180 37
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 675 1267 1284 1608
Size: 675 592 17 324
Fragments arranged by size:
675 592 324 17
TatI w'GTAC_w
Cuts at: 0 241 928 1044 1202 1608
Size: 241 687 116 158 406
Fragments arranged by size:
687 406 241 158 116
Enzymes that do cut:
AccI Acc65I AcuI AflIII AlfI AloI AlwNI ApaI
ApoI AvrII BaeI BamHI BanI BanII BcgI BciVI
BfrBI BglI BlpI Bme1580I BmrI BplI BpmI BpuEI
BsaHI BsaXI BseRI BseYI BsiHKAI Bsp1286I BsrBI BsrDI
BsrGI BstAPI BstYI Bsu36I BtgI BtsI DraIII EaeI
EciI Eco57MI EcoNI EcoO109I Hin4I HincII HindIII KpnI
MfeI MscI MslI MspA1I NcoI NsiI PflMI PfoI
PpuMI PshAI PspOMI PsrI PstI PvuII SalI ScaI
SfcI SmlI StuI StyI TaqII TatI
Enzymes that do not cut:
AarI AatII AclI AfeI AflII AgeI AhdI AleI
ApaLI AscI AseI AsiSI AvaI BbeI BbsI BbvCI
BclI BglII BmgBI BmtI Bpu10I BsaI BsaAI BsaBI
BsaWI BsgI BsiEI BsiWI BsmI BsmBI BspEI BspHI
BspMI BsrFI BssHII BssSI BstBI BstEII BstXI BstZ17I
BtgZI ClaI CspCI DraI DrdI EagI EarI EcoICRI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HpaI
KasI MluI MmeI NaeI NarI NdeI NgoMIV NheI
NotI NruI NspI PacI PasI PciI PmeI PmlI
PpiI PsiI PspXI PvuI RsrII SacI SacII SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SphI SrfI SspI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI XmnI ZraI