Restriction map for
pF1KB3580
(Linear) (Six-base) MAPSORT of: KIBB3580.seq Check: 4792 from: 1 to: 1614
KIBB3580.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 463 1614
Size: 449 14 1151
Fragments arranged by size:
1151 449 14
AlwNI CAG_nnn'CTG
Cuts at: 0 508 1614
Size: 508 1106
ApaI G_GGCC'C
Cuts at: 0 831 1614
Size: 831 783
ApoI r'AATT_y
Cuts at: 0 313 471 742 863 1177 1614
Size: 313 158 271 121 314 437
Fragments arranged by size:
437 314 313 271 158 121
AseI AT'TA_AT
Cuts at: 0 215 1614
Size: 215 1399
BanI G'GyrC_C
Cuts at: 0 231 1614
Size: 231 1383
BanII G_rGCy'C
Cuts at: 0 576 831 1614
Size: 576 255 783
Fragments arranged by size:
783 576 255
BbsI GAAGACnn'nnnn_
Cuts at: 0 1532 1614
Size: 1532 82
BfrBI ATG'CAT
Cuts at: 0 918 1614
Size: 918 696
BglI GCCn_nnn'nGGC
Cuts at: 0 826 1614
Size: 826 788
BglII A'GATC_T
Cuts at: 0 1472 1614
Size: 1472 142
BlpI GC'TnA_GC
Cuts at: 0 532 1011 1614
Size: 532 479 603
Fragments arranged by size:
603 532 479
Bme1580I G_kGCm'C
Cuts at: 0 515 831 1113 1614
Size: 515 316 282 501
Fragments arranged by size:
515 501 316 282
BmrI ACTGGGnnnn_n'
Cuts at: 0 1273 1614
Size: 1273 341
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 611 722 1247 1614
Size: 611 111 525 367
Fragments arranged by size:
611 525 367 111
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 93 1282 1614
Size: 93 1189 332
Fragments arranged by size:
1189 332 93
BseYI C'CCAG_C
Cuts at: 0 824 1146 1614
Size: 824 322 468
Fragments arranged by size:
824 468 322
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 627 1614
Size: 627 987
BsmI GAATG_Cn'
Cuts at: 0 696 1614
Size: 696 918
Bsp1286I G_dGCh'C
Cuts at: 0 515 576 831 1113 1614
Size: 515 61 255 282 501
Fragments arranged by size:
515 501 282 255 61
BspHI T'CATG_A
Cuts at: 0 201 1458 1614
Size: 201 1257 156
Fragments arranged by size:
1257 201 156
BsrBI CCG'CTC
Cuts at: 0 1281 1614
Size: 1281 333
BsrDI GCAATG_nn'
Cuts at: 0 584 1535 1614
Size: 584 951 79
Fragments arranged by size:
951 584 79
BsrGI T'GTAC_A
Cuts at: 0 421 1317 1614
Size: 421 896 297
Fragments arranged by size:
896 421 297
BstXI CCAn_nnnn'nTGG
Cuts at: 0 393 1614
Size: 393 1221
BstYI r'GATC_y
Cuts at: 0 1115 1472 1614
Size: 1115 357 142
Fragments arranged by size:
1115 357 142
Bsu36I CC'TnA_GG
Cuts at: 0 1351 1614
Size: 1351 263
BtsI GCAGTG_nn'
Cuts at: 0 424 1614
Size: 424 1190
ClaI AT'CG_AT
Cuts at: 0 878 1614
Size: 878 736
DraIII CAC_nnn'GTG
Cuts at: 0 238 1614
Size: 238 1376
EaeI y'GGCC_r
Cuts at: 0 383 1614
Size: 383 1231
EarI CTCTTCn'nnn_
Cuts at: 0 116 482 1614
Size: 116 366 1132
Fragments arranged by size:
1132 366 116
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 449 463 611 722 1247 1614
Size: 449 14 148 111 525 367
Fragments arranged by size:
525 449 367 148 111 14
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1598 1614
Size: 1598 16
EcoO109I rG'GnC_Cy
Cuts at: 0 828 1614
Size: 828 786
EcoRV GAT'ATC
Cuts at: 0 1085 1614
Size: 1085 529
FspI TGC'GCA
Cuts at: 0 73 1614
Size: 73 1541
HincII GTy'rAC
Cuts at: 0 1551 1614
Size: 1551 63
HindIII A'AGCT_T
Cuts at: 0 1421 1614
Size: 1421 193
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1057 1602 1614
Size: 1057 545 12
Fragments arranged by size:
1057 545 12
MscI TGG'CCA
Cuts at: 0 385 1614
Size: 385 1229
MslI CAynn'nnrTG
Cuts at: 0 226 1300 1614
Size: 226 1074 314
Fragments arranged by size:
1074 314 226
MspA1I CmG'CkG
Cuts at: 0 1099 1237 1614
Size: 1099 138 377
Fragments arranged by size:
1099 377 138
NsiI A_TGCA'T
Cuts at: 0 920 1614
Size: 920 694
NspI r_CATG'y
Cuts at: 0 1076 1614
Size: 1076 538
PfoI T'CCnGG_A
Cuts at: 0 554 1614
Size: 554 1060
PsiI TTA'TAA
Cuts at: 0 929 1614
Size: 929 685
PspOMI G'GGCC_C
Cuts at: 0 827 1614
Size: 827 787
PstI C_TGCA'G
Cuts at: 0 101 431 1022 1614
Size: 101 330 591 592
Fragments arranged by size:
592 591 330 101
PvuII CAG'CTG
Cuts at: 0 1099 1237 1614
Size: 1099 138 377
Fragments arranged by size:
1099 377 138
SapI GCTCTTCn'nnn_
Cuts at: 0 116 1614
Size: 116 1498
SexAI A'CCwGG_T
Cuts at: 0 234 1614
Size: 234 1380
SfcI C'TryA_G
Cuts at: 0 97 152 427 717 1018 1614
Size: 97 55 275 290 301 596
Fragments arranged by size:
596 301 290 275 97 55
SspI AAT'ATT
Cuts at: 0 459 1092 1614
Size: 459 633 522
Fragments arranged by size:
633 522 459
StuI AGG'CCT
Cuts at: 0 1486 1614
Size: 1486 128
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1531 1614
Size: 1531 83
TatI w'GTAC_w
Cuts at: 0 421 807 1317 1568 1614
Size: 421 386 510 251 46
Fragments arranged by size:
510 421 386 251 46
Tth111I GACn'n_nGTC
Cuts at: 0 616 1614
Size: 616 998
Enzymes that do cut:
AcuI AlwNI ApaI ApoI AseI BanI BanII BbsI
BfrBI BglI BglII BlpI Bme1580I BmrI BpmI BseRI
BseYI BsgI BsmI Bsp1286I BspHI BsrBI BsrDI BsrGI
BstXI BstYI Bsu36I BtsI ClaI DraIII EaeI EarI
Eco57MI EcoNI EcoO109I EcoRV FspI HincII HindIII MmeI
MscI MslI MspA1I NsiI NspI PfoI PsiI PspOMI
PstI PvuII SapI SexAI SfcI SspI StuI TaqII
TatI Tth111I
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApaLI AscI AsiSI
AvaI AvrII BaeI BamHI BbeI BbvCI BcgI BciVI
BclI BmgBI BmtI BplI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmBI
BspEI BspMI BsrFI BssHII BssSI BstAPI BstBI BstEII
BstZ17I BtgI BtgZI CspCI DraI DrdI EagI EciI
EcoICRI EcoRI FalI FseI FspAI HaeII Hin4I HpaI
KasI KpnI MfeI MluI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SfiI
SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI
SrfI StyI SwaI TstI XbaI XcmI XhoI XmaI
XmnI ZraI