Restriction map for
pF1KB3596
(Linear) (Six-base) MAPSORT of: KIBB3596.seq Check: 418 from: 1 to: 1509
KIBB3596.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
Acc65I G'GTAC_C
Cuts at: 0 964 1509
Size: 964 545
AclI AA'CG_TT
Cuts at: 0 717 1509
Size: 717 792
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1509
Size: 1163 346
AlwNI CAG_nnn'CTG
Cuts at: 0 565 892 1509
Size: 565 327 617
Fragments arranged by size:
617 565 327
ApaLI G'TGCA_C
Cuts at: 0 257 1509
Size: 257 1252
ApoI r'AATT_y
Cuts at: 0 1256 1509
Size: 1256 253
AvaI C'yCGr_G
Cuts at: 0 84 236 1509
Size: 84 152 1273
Fragments arranged by size:
1273 152 84
BamHI G'GATC_C
Cuts at: 0 856 1509
Size: 856 653
BanI G'GyrC_C
Cuts at: 0 964 1509
Size: 964 545
BanII G_rGCy'C
Cuts at: 0 158 1509
Size: 158 1351
Bme1580I G_kGCm'C
Cuts at: 0 261 1509
Size: 261 1248
BmrI ACTGGGnnnn_n'
Cuts at: 0 284 1509
Size: 284 1225
Bpu10I CC'TnA_GC
Cuts at: 0 570 1509
Size: 570 939
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1410 1509
Size: 1410 99
BsaI GGTCTCn'nnnn_
Cuts at: 0 172 1509
Size: 172 1337
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 668 698 1509
Size: 668 30 811
Fragments arranged by size:
811 668 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 28 31 693 1509
Size: 28 3 662 816
Fragments arranged by size:
816 662 28 3
BsiEI CG_ry'CG
Cuts at: 0 14 1509
Size: 14 1495
BsiHKAI G_wGCw'C
Cuts at: 0 261 1509
Size: 261 1248
Bsp1286I G_dGCh'C
Cuts at: 0 158 261 1509
Size: 158 103 1248
Fragments arranged by size:
1248 158 103
BspHI T'CATG_A
Cuts at: 0 765 846 1260 1509
Size: 765 81 414 249
Fragments arranged by size:
765 414 249 81
BsrBI CCG'CTC
Cuts at: 0 932 1509
Size: 932 577
BsrDI GCAATG_nn'
Cuts at: 0 312 386 580 983 1214 1453 1509
Size: 312 74 194 403 231 239 56
Fragments arranged by size:
403 312 239 231 194 74 56
BsrGI T'GTAC_A
Cuts at: 0 121 1509
Size: 121 1388
BssSI C'ACGA_G
Cuts at: 0 43 1509
Size: 43 1466
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1370 1509
Size: 1370 139
BstYI r'GATC_y
Cuts at: 0 856 1509
Size: 856 653
BtgI C'CryG_G
Cuts at: 0 923 1509
Size: 923 586
DraIII CAC_nnn'GTG
Cuts at: 0 1116 1509
Size: 1116 393
EarI CTCTTCn'nnn_
Cuts at: 0 270 476 1509
Size: 270 206 1033
Fragments arranged by size:
1033 270 206
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1163 1509
Size: 1163 346
EcoO109I rG'GnC_Cy
Cuts at: 0 486 1509
Size: 486 1023
EcoRI G'AATT_C
Cuts at: 0 1256 1509
Size: 1256 253
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1032 1509
Size: 1032 477
HaeII r_GCGC'y
Cuts at: 0 79 1509
Size: 79 1430
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 841 873 988 1020 1509
Size: 841 32 115 32 489
Fragments arranged by size:
841 489 115 32 32
HincII GTy'rAC
Cuts at: 0 1489 1509
Size: 1489 20
KpnI G_GTAC'C
Cuts at: 0 968 1509
Size: 968 541
MfeI C'AATT_G
Cuts at: 0 599 1509
Size: 599 910
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 358 1509
Size: 358 1151
MslI CAynn'nnrTG
Cuts at: 0 427 851 1165 1504 1509
Size: 427 424 314 339 5
Fragments arranged by size:
427 424 339 314 5
MspA1I CmG'CkG
Cuts at: 0 382 1509
Size: 382 1127
NspI r_CATG'y
Cuts at: 0 210 1509
Size: 210 1299
PflMI CCAn_nnn'nTGG
Cuts at: 0 1116 1509
Size: 1116 393
PpuMI rG'GwC_Cy
Cuts at: 0 486 1509
Size: 486 1023
PvuII CAG'CTG
Cuts at: 0 382 1509
Size: 382 1127
SmaI CCC'GGG
Cuts at: 0 86 1509
Size: 86 1423
SmlI C'TyrA_G
Cuts at: 0 236 1389 1509
Size: 236 1153 120
Fragments arranged by size:
1153 236 120
StyI C'CwwG_G
Cuts at: 0 360 969 1509
Size: 360 609 540
Fragments arranged by size:
609 540 360
TatI w'GTAC_w
Cuts at: 0 121 1509
Size: 121 1388
Tth111I GACn'n_nGTC
Cuts at: 0 555 1509
Size: 555 954
XbaI T'CTAG_A
Cuts at: 0 706 1509
Size: 706 803
XhoI C'TCGA_G
Cuts at: 0 236 1509
Size: 236 1273
XmaI C'CCGG_G
Cuts at: 0 84 1509
Size: 84 1425
Enzymes that do cut:
Acc65I AclI AcuI AlwNI ApaLI ApoI AvaI BamHI
BanI BanII Bme1580I BmrI Bpu10I BpuEI BsaI BsaXI
BseRI BsiEI BsiHKAI Bsp1286I BspHI BsrBI BsrDI BsrGI
BssSI BstXI BstYI BtgI DraIII EarI Eco57MI EcoO109I
EcoRI FalI HaeII Hin4I HincII KpnI MfeI MmeI
MslI MspA1I NspI PflMI PpuMI PvuII SmaI SmlI
StyI TatI Tth111I XbaI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApaI AscI AseI AsiSI AvrII
BaeI BbeI BbsI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BpmI BsaAI
BsaBI BsaHI BsaWI BseYI BsgI BsiWI BsmI BsmBI
BspEI BspMI BsrFI BssHII BstAPI BstBI BstEII BstZ17I
Bsu36I BtgZI BtsI ClaI CspCI DraI DrdI EaeI
EagI EciI EcoICRI EcoNI EcoRV FseI FspI FspAI
HindIII HpaI KasI MluI MscI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PstI PvuI RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TstI XcmI XmnI ZraI