Restriction map for pF1KB3604
 (Linear) (Six-base) MAPSORT of: KIBB3604.seq  Check: 6774  from: 1  to: 1500



KIBB3604.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0     104    1500 
   Size:        104    1396
 
Acc65I G'GTAC_C

Cuts at:      0     533    1274    1500 
   Size:        533     741     226

  Fragments arranged by size: 

                 741     533     226
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0    1047    1422    1500 
   Size:       1047     375      78

  Fragments arranged by size: 

                1047     375      78
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     292    1500 
   Size:        292    1208
 
AlwNI CAG_nnn'CTG

Cuts at:      0      27     208    1500 
   Size:         27     181    1292

  Fragments arranged by size: 

                1292     181      27
 
ApaI G_GGCC'C

Cuts at:      0    1020    1500 
   Size:       1020     480
 
ApoI r'AATT_y

Cuts at:      0     112    1500 
   Size:        112    1388
 
BamHI G'GATC_C

Cuts at:      0      70    1500 
   Size:         70    1430
 
BanI G'GyrC_C

Cuts at:      0      63     533    1274    1309    1500 
   Size:         63     470     741      35     191

  Fragments arranged by size: 

                 741     470     191      63      35
 
BanII G_rGCy'C

Cuts at:      0     881    1020    1500 
   Size:        881     139     480

  Fragments arranged by size: 

                 881     480     139
 
BbsI GAAGACnn'nnnn_

Cuts at:      0     145     859    1428    1500 
   Size:        145     714     569      72

  Fragments arranged by size: 

                 714     569     145      72
 
BbvCI CC'TCA_GC

Cuts at:      0    1178    1500 
   Size:       1178     322
 
BglI GCCn_nnn'nGGC

Cuts at:      0    1237    1500 
   Size:       1237     263
 
BglII A'GATC_T

Cuts at:      0     160     296    1500 
   Size:        160     136    1204

  Fragments arranged by size: 

                1204     160     136
 
BlpI GC'TnA_GC

Cuts at:      0     672    1500 
   Size:        672     828
 
Bme1580I G_kGCm'C

Cuts at:      0    1020    1500 
   Size:       1020     480
 
BmgBI CAC'GTC

Cuts at:      0     587    1053    1500 
   Size:        587     466     447

  Fragments arranged by size: 

                 587     466     447
 
BmrI ACTGGGnnnn_n'

Cuts at:      0    1038    1091    1275    1500 
   Size:       1038      53     184     225

  Fragments arranged by size: 

                1038     225     184      53
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601    1249    1500 
   Size:        601     648     251

  Fragments arranged by size: 

                 648     601     251
 
Bpu10I CC'TnA_GC

Cuts at:      0    1178    1500 
   Size:       1178     322
 
BsaBI GATnn'nnATC

Cuts at:      0     709    1500 
   Size:        709     791
 
BsaWI w'CCGG_w

Cuts at:      0     405     536     988    1127    1500 
   Size:        405     131     452     139     373

  Fragments arranged by size: 

                 452     405     373     139     131
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     291     589     808    1007    1117    1500 
   Size:        291     298     219     199     110     383

  Fragments arranged by size: 

                 383     298     291     219     199     110
 
BseYI C'CCAG_C

Cuts at:      0      20     467    1044    1146    1500 
   Size:         20     447     577     102     354

  Fragments arranged by size: 

                 577     447     354     102      20
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     984    1500 
   Size:        984     516
 
BsiHKAI G_wGCw'C

Cuts at:      0    1028    1500 
   Size:       1028     472
 
BsmI GAATG_Cn'

Cuts at:      0     204     281    1500 
   Size:        204      77    1219

  Fragments arranged by size: 

                1219     204      77
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0     577    1500 
   Size:        577     923
 
Bsp1286I G_dGCh'C

Cuts at:      0     881    1020    1028    1500 
   Size:        881     139       8     472

  Fragments arranged by size: 

                 881     472     139       8
 
BspEI T'CCGG_A

Cuts at:      0     405    1500 
   Size:        405    1095
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0    1217    1500 
   Size:       1217     283
 
BstAPI GCAn_nnn'nTGC

Cuts at:      0     438    1228    1316    1500 
   Size:        438     790      88     184

  Fragments arranged by size: 

                 790     438     184      88
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     607    1500 
   Size:        607     893
 
BstYI r'GATC_y

Cuts at:      0      70     160     282     296     822    1130    1500 
   Size:         70      90     122      14     526     308     370

  Fragments arranged by size: 

                 526     370     308     122      90      70      14
 
BtgI C'CryG_G

Cuts at:      0     479     651     662     745    1500 
   Size:        479     172      11      83     755

  Fragments arranged by size: 

                 755     479     172      83      11
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     717     927    1500 
   Size:        717     210     573

  Fragments arranged by size: 

                 717     573     210
 
DrdI GACnn_nn'nnGTC

Cuts at:      0      83    1500 
   Size:         83    1417
 
EaeI y'GGCC_r

Cuts at:      0     860    1500 
   Size:        860     640
 
EarI CTCTTCn'nnn_

Cuts at:      0     227     761     806    1500 
   Size:        227     534      45     694

  Fragments arranged by size: 

                 694     534     227      45
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     601    1047    1249    1422    1500 
   Size:        601     446     202     173      78

  Fragments arranged by size: 

                 601     446     202     173      78
 
EcoO109I rG'GnC_Cy

Cuts at:      0     154     603    1032    1152    1206    1239    1500 
   Size:        154     449     429     120      54      33     261

  Fragments arranged by size: 

                 449     429     261     154     120      54      33
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     540    1500 
   Size:        540     960
 
HaeII r_GCGC'y

Cuts at:      0      27    1500 
   Size:         27    1473
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     374     406    1500 
   Size:        374      32    1094

  Fragments arranged by size: 

                1094     374      32
 
HincII GTy'rAC

Cuts at:      0     105     378    1500 
   Size:        105     273    1122

  Fragments arranged by size: 

                1122     273     105
 
KpnI G_GTAC'C

Cuts at:      0     537    1278    1500 
   Size:        537     741     222

  Fragments arranged by size: 

                 741     537     222
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     372    1500 
   Size:        372    1128
 
MslI CAynn'nnrTG

Cuts at:      0     478     526    1500 
   Size:        478      48     974

  Fragments arranged by size: 

                 974     478      48
 
MspA1I CmG'CkG

Cuts at:      0     183     803    1500 
   Size:        183     620     697

  Fragments arranged by size: 

                 697     620     183
 
NcoI C'CATG_G

Cuts at:      0     479     651     662    1500 
   Size:        479     172      11     838

  Fragments arranged by size: 

                 838     479     172      11
 
NspI r_CATG'y

Cuts at:      0     372    1500 
   Size:        372    1128
 
PasI CC'CwG_GG

Cuts at:      0     151    1500 
   Size:        151    1349
 
PflMI CCAn_nnn'nTGG

Cuts at:      0      27    1500 
   Size:         27    1473
 
PpuMI rG'GwC_Cy

Cuts at:      0    1152    1206    1500 
   Size:       1152      54     294

  Fragments arranged by size: 

                1152     294      54
 
PshAI GACnn'nnGTC

Cuts at:      0     145    1500 
   Size:        145    1355
 
PspOMI G'GGCC_C

Cuts at:      0    1016    1500 
   Size:       1016     484
 
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'

Cuts at:      0    1267    1299    1500 
   Size:       1267      32     201

  Fragments arranged by size: 

                1267     201      32
 
PstI C_TGCA'G

Cuts at:      0      37      96    1500 
   Size:         37      59    1404

  Fragments arranged by size: 

                1404      59      37
 
PvuII CAG'CTG

Cuts at:      0     183    1500 
   Size:        183    1317
 
SalI G'TCGA_C

Cuts at:      0     103    1500 
   Size:        103    1397
 
SanDI GG'GwC_CC

Cuts at:      0    1152    1500 
   Size:       1152     348
 
SexAI A'CCwGG_T

Cuts at:      0     251    1500 
   Size:        251    1249
 
SfcI C'TryA_G

Cuts at:      0      33      92     621    1500 
   Size:         33      59     529     879

  Fragments arranged by size: 

                 879     529      59      33
 
SspI AAT'ATT

Cuts at:      0    1470    1500 
   Size:       1470      30
 
StuI AGG'CCT

Cuts at:      0    1113    1500 
   Size:       1113     387
 
StyI C'CwwG_G

Cuts at:      0     479     651     662    1500 
   Size:        479     172      11     838

  Fragments arranged by size: 

                 838     479     172      11
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0    1381    1500 
   Size:       1381     119
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     892    1500 
   Size:        892     608
 

 Enzymes that do cut:

     AccI   Acc65I     AcuI     AlfI    AlwNI     ApaI     ApoI    BamHI
     BanI    BanII     BbsI    BbvCI     BglI    BglII     BlpI Bme1580I
    BmgBI     BmrI     BpmI   Bpu10I    BsaBI    BsaWI    BseRI    BseYI
     BsgI  BsiHKAI     BsmI    BsmBI Bsp1286I    BspEI    BspMI   BstAPI
    BstXI    BstYI     BtgI    BtgZI     DrdI     EaeI     EarI  Eco57MI
 EcoO109I     FalI    HaeII    Hin4I   HincII     KpnI     MmeI     MslI
   MspA1I     NcoI     NspI     PasI    PflMI    PpuMI    PshAI   PspOMI
     PsrI     PstI    PvuII     SalI    SanDI    SexAI     SfcI     SspI
     StuI     StyI    TaqII     XcmI

 Enzymes that do not cut: 

     AarI    AatII     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AloI    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII
     BaeI     BbeI     BcgI    BciVI     BclI    BfrBI     BmtI     BplI
    BpuEI     BsaI    BsaAI    BsaHI    BsaXI    BsiEI    BsiWI    BspHI
    BsrBI    BsrDI    BsrFI    BsrGI   BssHII    BssSI    BstBI   BstEII
  BstZ17I   Bsu36I     BtsI     ClaI    CspCI     DraI   DraIII     EagI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FseI     FspI    FspAI
  HindIII     HpaI     KasI     MfeI     MluI     MscI     NaeI     NarI
     NdeI   NgoMIV     NheI     NotI     NruI     NsiI     PacI     PciI
     PfoI     PmeI     PmlI     PpiI     PsiI    PspXI     PvuI    RsrII
     SacI    SacII     SapI     SbfI     ScaI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SwaI
     TatI     TstI  Tth111I     XbaI     XhoI     XmaI     XmnI     ZraI
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