(Linear) (Six-base) MAPSORT of: KIBB3604.seq Check: 6774 from: 1 to: 1500 KIBB3604.orf With 182 enzymes: SgfI * April 24, 2009 13:02 .. AccI GT'mk_AC Cuts at: 0 104 1500 Size: 104 1396 Acc65I G'GTAC_C Cuts at: 0 533 1274 1500 Size: 533 741 226 Fragments arranged by size: 741 533 226 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 0 1047 1422 1500 Size: 1047 375 78 Fragments arranged by size: 1047 375 78 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 0 292 1500 Size: 292 1208 AlwNI CAG_nnn'CTG Cuts at: 0 27 208 1500 Size: 27 181 1292 Fragments arranged by size: 1292 181 27 ApaI G_GGCC'C Cuts at: 0 1020 1500 Size: 1020 480 ApoI r'AATT_y Cuts at: 0 112 1500 Size: 112 1388 BamHI G'GATC_C Cuts at: 0 70 1500 Size: 70 1430 BanI G'GyrC_C Cuts at: 0 63 533 1274 1309 1500 Size: 63 470 741 35 191 Fragments arranged by size: 741 470 191 63 35 BanII G_rGCy'C Cuts at: 0 881 1020 1500 Size: 881 139 480 Fragments arranged by size: 881 480 139 BbsI GAAGACnn'nnnn_ Cuts at: 0 145 859 1428 1500 Size: 145 714 569 72 Fragments arranged by size: 714 569 145 72 BbvCI CC'TCA_GC Cuts at: 0 1178 1500 Size: 1178 322 BglI GCCn_nnn'nGGC Cuts at: 0 1237 1500 Size: 1237 263 BglII A'GATC_T Cuts at: 0 160 296 1500 Size: 160 136 1204 Fragments arranged by size: 1204 160 136 BlpI GC'TnA_GC Cuts at: 0 672 1500 Size: 672 828 Bme1580I G_kGCm'C Cuts at: 0 1020 1500 Size: 1020 480 BmgBI CAC'GTC Cuts at: 0 587 1053 1500 Size: 587 466 447 Fragments arranged by size: 587 466 447 BmrI ACTGGGnnnn_n' Cuts at: 0 1038 1091 1275 1500 Size: 1038 53 184 225 Fragments arranged by size: 1038 225 184 53 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 601 1249 1500 Size: 601 648 251 Fragments arranged by size: 648 601 251 Bpu10I CC'TnA_GC Cuts at: 0 1178 1500 Size: 1178 322 BsaBI GATnn'nnATC Cuts at: 0 709 1500 Size: 709 791 BsaWI w'CCGG_w Cuts at: 0 405 536 988 1127 1500 Size: 405 131 452 139 373 Fragments arranged by size: 452 405 373 139 131 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 0 291 589 808 1007 1117 1500 Size: 291 298 219 199 110 383 Fragments arranged by size: 383 298 291 219 199 110 BseYI C'CCAG_C Cuts at: 0 20 467 1044 1146 1500 Size: 20 447 577 102 354 Fragments arranged by size: 577 447 354 102 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 0 984 1500 Size: 984 516 BsiHKAI G_wGCw'C Cuts at: 0 1028 1500 Size: 1028 472 BsmI GAATG_Cn' Cuts at: 0 204 281 1500 Size: 204 77 1219 Fragments arranged by size: 1219 204 77 BsmBI CGTCTCn'nnnn_ Cuts at: 0 577 1500 Size: 577 923 Bsp1286I G_dGCh'C Cuts at: 0 881 1020 1028 1500 Size: 881 139 8 472 Fragments arranged by size: 881 472 139 8 BspEI T'CCGG_A Cuts at: 0 405 1500 Size: 405 1095 BspMI ACCTGCnnnn'nnnn_ Cuts at: 0 1217 1500 Size: 1217 283 BstAPI GCAn_nnn'nTGC Cuts at: 0 438 1228 1316 1500 Size: 438 790 88 184 Fragments arranged by size: 790 438 184 88 BstXI CCAn_nnnn'nTGG Cuts at: 0 607 1500 Size: 607 893 BstYI r'GATC_y Cuts at: 0 70 160 282 296 822 1130 1500 Size: 70 90 122 14 526 308 370 Fragments arranged by size: 526 370 308 122 90 70 14 BtgI C'CryG_G Cuts at: 0 479 651 662 745 1500 Size: 479 172 11 83 755 Fragments arranged by size: 755 479 172 83 11 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 0 717 927 1500 Size: 717 210 573 Fragments arranged by size: 717 573 210 DrdI GACnn_nn'nnGTC Cuts at: 0 83 1500 Size: 83 1417 EaeI y'GGCC_r Cuts at: 0 860 1500 Size: 860 640 EarI CTCTTCn'nnn_ Cuts at: 0 227 761 806 1500 Size: 227 534 45 694 Fragments arranged by size: 694 534 227 45 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 0 601 1047 1249 1422 1500 Size: 601 446 202 173 78 Fragments arranged by size: 601 446 202 173 78 EcoO109I rG'GnC_Cy Cuts at: 0 154 603 1032 1152 1206 1239 1500 Size: 154 449 429 120 54 33 261 Fragments arranged by size: 449 429 261 154 120 54 33 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 0 540 1500 Size: 540 960 HaeII r_GCGC'y Cuts at: 0 27 1500 Size: 27 1473 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 374 406 1500 Size: 374 32 1094 Fragments arranged by size: 1094 374 32 HincII GTy'rAC Cuts at: 0 105 378 1500 Size: 105 273 1122 Fragments arranged by size: 1122 273 105 KpnI G_GTAC'C Cuts at: 0 537 1278 1500 Size: 537 741 222 Fragments arranged by size: 741 537 222 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 372 1500 Size: 372 1128 MslI CAynn'nnrTG Cuts at: 0 478 526 1500 Size: 478 48 974 Fragments arranged by size: 974 478 48 MspA1I CmG'CkG Cuts at: 0 183 803 1500 Size: 183 620 697 Fragments arranged by size: 697 620 183 NcoI C'CATG_G Cuts at: 0 479 651 662 1500 Size: 479 172 11 838 Fragments arranged by size: 838 479 172 11 NspI r_CATG'y Cuts at: 0 372 1500 Size: 372 1128 PasI CC'CwG_GG Cuts at: 0 151 1500 Size: 151 1349 PflMI CCAn_nnn'nTGG Cuts at: 0 27 1500 Size: 27 1473 PpuMI rG'GwC_Cy Cuts at: 0 1152 1206 1500 Size: 1152 54 294 Fragments arranged by size: 1152 294 54 PshAI GACnn'nnGTC Cuts at: 0 145 1500 Size: 145 1355 PspOMI G'GGCC_C Cuts at: 0 1016 1500 Size: 1016 484 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 0 1267 1299 1500 Size: 1267 32 201 Fragments arranged by size: 1267 201 32 PstI C_TGCA'G Cuts at: 0 37 96 1500 Size: 37 59 1404 Fragments arranged by size: 1404 59 37 PvuII CAG'CTG Cuts at: 0 183 1500 Size: 183 1317 SalI G'TCGA_C Cuts at: 0 103 1500 Size: 103 1397 SanDI GG'GwC_CC Cuts at: 0 1152 1500 Size: 1152 348 SexAI A'CCwGG_T Cuts at: 0 251 1500 Size: 251 1249 SfcI C'TryA_G Cuts at: 0 33 92 621 1500 Size: 33 59 529 879 Fragments arranged by size: 879 529 59 33 SspI AAT'ATT Cuts at: 0 1470 1500 Size: 1470 30 StuI AGG'CCT Cuts at: 0 1113 1500 Size: 1113 387 StyI C'CwwG_G Cuts at: 0 479 651 662 1500 Size: 479 172 11 838 Fragments arranged by size: 838 479 172 11 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 0 1381 1500 Size: 1381 119 XcmI CCAnnnn_n'nnnnTGG Cuts at: 0 892 1500 Size: 892 608 Enzymes that do cut: AccI Acc65I AcuI AlfI AlwNI ApaI ApoI BamHI BanI BanII BbsI BbvCI BglI BglII BlpI Bme1580I BmgBI BmrI BpmI Bpu10I BsaBI BsaWI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspMI BstAPI BstXI BstYI BtgI BtgZI DrdI EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII KpnI MmeI MslI MspA1I NcoI NspI PasI PflMI PpuMI PshAI PspOMI PsrI PstI PvuII SalI SanDI SexAI SfcI SspI StuI StyI TaqII XcmI Enzymes that do not cut: AarI AatII AclI AfeI AflII AflIII AgeI AhdI AleI AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI BbeI BcgI BciVI BclI BfrBI BmtI BplI BpuEI BsaI BsaAI BsaHI BsaXI BsiEI BsiWI BspHI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstBI BstEII BstZ17I Bsu36I BtsI ClaI CspCI DraI DraIII EagI EciI EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HindIII HpaI KasI MfeI MluI MscI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PciI PfoI PmeI PmlI PpiI PsiI PspXI PvuI RsrII SacI SacII SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SwaI TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI