Restriction map for
pF1KB3619
(Linear) (Six-base) MAPSORT of: KIBB3619.seq Check: 9824 from: 1 to: 2706
KIBB3619.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
Acc65I G'GTAC_C
Cuts at: 0 398 1628 2706
Size: 398 1230 1078
Fragments arranged by size:
1230 1078 398
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 1318 2182 2387 2706
Size: 171 1147 864 205 319
Fragments arranged by size:
1147 864 319 205 171
AflIII A'CryG_T
Cuts at: 0 1137 2706
Size: 1137 1569
AgeI A'CCGG_T
Cuts at: 0 1454 2706
Size: 1454 1252
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1402 1434 2251 2283 2706
Size: 1402 32 817 32 423
Fragments arranged by size:
1402 817 423 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 245 2706
Size: 245 2461
ApoI r'AATT_y
Cuts at: 0 251 477 495 1348 1502 2545 2706
Size: 251 226 18 853 154 1043 161
Fragments arranged by size:
1043 853 251 226 161 154 18
AseI AT'TA_AT
Cuts at: 0 1319 2706
Size: 1319 1387
AvaI C'yCGr_G
Cuts at: 0 1944 2706
Size: 1944 762
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 996 1029 1612 1645 2706
Size: 996 33 583 33 1061
Fragments arranged by size:
1061 996 583 33 33
BanI G'GyrC_C
Cuts at: 0 398 1628 2179 2706
Size: 398 1230 551 527
Fragments arranged by size:
1230 551 527 398
BanII G_rGCy'C
Cuts at: 0 27 83 2297 2706
Size: 27 56 2214 409
Fragments arranged by size:
2214 409 56 27
BbsI GAAGACnn'nnnn_
Cuts at: 0 464 2129 2593 2706
Size: 464 1665 464 113
Fragments arranged by size:
1665 464 464 113
BciVI GTATCCnnnnn_n'
Cuts at: 0 2313 2706
Size: 2313 393
BglII A'GATC_T
Cuts at: 0 2213 2706
Size: 2213 493
BmrI ACTGGGnnnn_n'
Cuts at: 0 524 2706
Size: 524 2182
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 746 2281 2706
Size: 746 1535 425
Fragments arranged by size:
1535 746 425
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 864 2706
Size: 864 1842
BsaI GGTCTCn'nnnn_
Cuts at: 0 670 2184 2706
Size: 670 1514 522
Fragments arranged by size:
1514 670 522
BsaAI yAC'GTr
Cuts at: 0 571 1140 2706
Size: 571 569 1566
Fragments arranged by size:
1566 571 569
BsaBI GATnn'nnATC
Cuts at: 0 800 2706
Size: 800 1906
BsaWI w'CCGG_w
Cuts at: 0 1454 1631 2706
Size: 1454 177 1075
Fragments arranged by size:
1454 1075 177
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1705 1735 2251 2281 2706
Size: 1705 30 516 30 425
Fragments arranged by size:
1705 516 425 30 30
BseYI C'CCAG_C
Cuts at: 0 26 2480 2706
Size: 26 2454 226
Fragments arranged by size:
2454 226 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1118 2706
Size: 1118 1588
BsiEI CG_ry'CG
Cuts at: 0 20 2706
Size: 20 2686
BsiHKAI G_wGCw'C
Cuts at: 0 850 2297 2706
Size: 850 1447 409
Fragments arranged by size:
1447 850 409
BsmI GAATG_Cn'
Cuts at: 0 918 2288 2327 2524 2706
Size: 918 1370 39 197 182
Fragments arranged by size:
1370 918 197 182 39
Bsp1286I G_dGCh'C
Cuts at: 0 27 83 850 2297 2706
Size: 27 56 767 1447 409
Fragments arranged by size:
1447 767 409 56 27
BspHI T'CATG_A
Cuts at: 0 1235 2706
Size: 1235 1471
BsrBI CCG'CTC
Cuts at: 0 12 2706
Size: 12 2694
BsrFI r'CCGG_y
Cuts at: 0 1454 2706
Size: 1454 1252
BstXI CCAn_nnnn'nTGG
Cuts at: 0 2278 2628 2706
Size: 2278 350 78
Fragments arranged by size:
2278 350 78
BstYI r'GATC_y
Cuts at: 0 2213 2459 2706
Size: 2213 246 247
Fragments arranged by size:
2213 247 246
Bsu36I CC'TnA_GG
Cuts at: 0 2608 2706
Size: 2608 98
BtgI C'CryG_G
Cuts at: 0 896 1840 2706
Size: 896 944 866
Fragments arranged by size:
944 896 866
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 2445 2706
Size: 2445 261
BtsI GCAGTG_nn'
Cuts at: 0 2371 2706
Size: 2371 335
DraIII CAC_nnn'GTG
Cuts at: 0 2361 2706
Size: 2361 345
EaeI y'GGCC_r
Cuts at: 0 753 2706
Size: 753 1953
EarI CTCTTCn'nnn_
Cuts at: 0 1597 2285 2596 2706
Size: 1597 688 311 110
Fragments arranged by size:
1597 688 311 110
EcoICRI GAG'CTC
Cuts at: 0 2295 2706
Size: 2295 411
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 171 746 1318 2182 2281 2387 2706
Size: 171 575 572 864 99 106 319
Fragments arranged by size:
864 575 572 319 171 106 99
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2137 2706
Size: 2137 569
EcoO109I rG'GnC_Cy
Cuts at: 0 67 2262 2706
Size: 67 2195 444
Fragments arranged by size:
2195 444 67
EcoRI G'AATT_C
Cuts at: 0 1502 2706
Size: 1502 1204
EcoRV GAT'ATC
Cuts at: 0 165 813 2706
Size: 165 648 1893
Fragments arranged by size:
1893 648 165
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 232 2706
Size: 232 2474
HaeII r_GCGC'y
Cuts at: 0 285 1589 2706
Size: 285 1304 1117
Fragments arranged by size:
1304 1117 285
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 51 83 1146 1178 2459 2491 2571 2603
Size: 51 32 1063 32 1281 32 80 32
Cuts at: 2603 2706
Size: 103
Fragments arranged by size:
1281 1063 103 80 51 32 32 32
32
HincII GTy'rAC
Cuts at: 0 708 1395 2706
Size: 708 687 1311
Fragments arranged by size:
1311 708 687
HindIII A'AGCT_T
Cuts at: 0 581 1385 2706
Size: 581 804 1321
Fragments arranged by size:
1321 804 581
KpnI G_GTAC'C
Cuts at: 0 402 1632 2706
Size: 402 1230 1074
Fragments arranged by size:
1230 1074 402
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 714 1014 2706
Size: 714 300 1692
Fragments arranged by size:
1692 714 300
MscI TGG'CCA
Cuts at: 0 755 2706
Size: 755 1951
MslI CAynn'nnrTG
Cuts at: 0 2501 2706
Size: 2501 205
MspA1I CmG'CkG
Cuts at: 0 331 657 2706
Size: 331 326 2049
Fragments arranged by size:
2049 331 326
NcoI C'CATG_G
Cuts at: 0 896 1840 2706
Size: 896 944 866
Fragments arranged by size:
944 896 866
NspI r_CATG'y
Cuts at: 0 157 1290 2706
Size: 157 1133 1416
Fragments arranged by size:
1416 1133 157
PflMI CCAn_nnn'nTGG
Cuts at: 0 837 2361 2706
Size: 837 1524 345
Fragments arranged by size:
1524 837 345
PfoI T'CCnGG_A
Cuts at: 0 74 2706
Size: 74 2632
PmlI CAC'GTG
Cuts at: 0 571 1140 2706
Size: 571 569 1566
Fragments arranged by size:
1566 571 569
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2251 2283 2706
Size: 2251 32 423
Fragments arranged by size:
2251 423 32
PpuMI rG'GwC_Cy
Cuts at: 0 67 2262 2706
Size: 67 2195 444
Fragments arranged by size:
2195 444 67
PsiI TTA'TAA
Cuts at: 0 1172 1433 2706
Size: 1172 261 1273
Fragments arranged by size:
1273 1172 261
PvuII CAG'CTG
Cuts at: 0 331 2706
Size: 331 2375
SacI G_AGCT'C
Cuts at: 0 2297 2706
Size: 2297 409
SapI GCTCTTCn'nnn_
Cuts at: 0 2285 2706
Size: 2285 421
ScaI AGT'ACT
Cuts at: 0 1831 2706
Size: 1831 875
SfcI C'TryA_G
Cuts at: 0 902 1022 2706
Size: 902 120 1684
Fragments arranged by size:
1684 902 120
SmlI C'TyrA_G
Cuts at: 0 843 2706
Size: 843 1863
SpeI A'CTAG_T
Cuts at: 0 2596 2706
Size: 2596 110
SphI G_CATG'C
Cuts at: 0 1290 2706
Size: 1290 1416
SspI AAT'ATT
Cuts at: 0 1365 2706
Size: 1365 1341
StuI AGG'CCT
Cuts at: 0 2613 2706
Size: 2613 93
StyI C'CwwG_G
Cuts at: 0 781 896 1840 2207 2706
Size: 781 115 944 367 499
Fragments arranged by size:
944 781 499 367 115
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1514 2634 2706
Size: 1514 1120 72
Fragments arranged by size:
1514 1120 72
TatI w'GTAC_w
Cuts at: 0 1829 2706
Size: 1829 877
XmnI GAAnn'nnTTC
Cuts at: 0 1967 2706
Size: 1967 739
Enzymes that do cut:
Acc65I AcuI AflIII AgeI AloI AlwNI ApoI AseI
AvaI BaeI BanI BanII BbsI BciVI BglII BmrI
BpmI BpuEI BsaI BsaAI BsaBI BsaWI BsaXI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspHI BsrBI BsrFI
BstXI BstYI Bsu36I BtgI BtgZI BtsI DraIII EaeI
EarI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI
HaeII Hin4I HincII HindIII KpnI MmeI MscI MslI
MspA1I NcoI NspI PflMI PfoI PmlI PpiI PpuMI
PsiI PvuII SacI SapI ScaI SfcI SmlI SpeI
SphI SspI StuI StyI TaqII TatI XmnI
Enzymes that do not cut:
AarI AatII AccI AclI AfeI AflII AhdI AleI
AlfI ApaI ApaLI AscI AsiSI AvrII BamHI BbeI
BbvCI BcgI BclI BfrBI BglI BlpI Bme1580I BmgBI
BmtI BplI Bpu10I BsaHI BseRI BsiWI BsmBI BspEI
BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstZ17I ClaI CspCI DraI DrdI EagI EciI FseI
FspI FspAI HpaI KasI MfeI MluI NaeI NarI
NdeI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PmeI PshAI PspOMI PspXI PsrI PstI PvuI
RsrII SacII SalI SanDI SbfI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SrfI SwaI TstI Tth111I
XbaI XcmI XhoI XmaI ZraI