(Linear) (Six-base) MAPSORT of: KIBB3630.seq  Check: 7711  from: 1  to: 579



KIBB3630.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AarI CACCTGCnnnn'nnnn_

Cuts at:      0     144     579 
   Size:        144     435
 
AccI GT'mk_AC

Cuts at:      0      98     579 
   Size:         98     481
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     579 
   Size:         57     522
 
AvaI C'yCGr_G

Cuts at:      0     524     579 
   Size:        524      55
 
BanI G'GyrC_C

Cuts at:      0      40     579 
   Size:         40     539
 
BanII G_rGCy'C

Cuts at:      0     413     579 
   Size:        413     166
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      58      63     197     579 
   Size:         58       5     134     382

  Fragments arranged by size: 

                 382     134      58       5
 
BglI GCCn_nnn'nGGC

Cuts at:      0     526     579 
   Size:        526      53
 
BlpI GC'TnA_GC

Cuts at:      0     477     579 
   Size:        477     102
 
Bme1580I G_kGCm'C

Cuts at:      0     332     579 
   Size:        332     247
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     520     579 
   Size:        520      59
 
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     490     579 
   Size:        490      89
 
BsaWI w'CCGG_w

Cuts at:      0      90     446     579 
   Size:         90     356     133

  Fragments arranged by size: 

                 356     133      90
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     552     579 
   Size:        552      27
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0      52     579 
   Size:         52     527
 
BsiHKAI G_wGCw'C

Cuts at:      0     377     478     579 
   Size:        377     101     101

  Fragments arranged by size: 

                 377     101     101
 
Bsp1286I G_dGCh'C

Cuts at:      0     332     377     413     478     579 
   Size:        332      45      36      65     101

  Fragments arranged by size: 

                 332     101      65      45      36
 
BspEI T'CCGG_A

Cuts at:      0      90     579 
   Size:         90     489
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0      64     144     579 
   Size:         64      80     435

  Fragments arranged by size: 

                 435      80      64
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     339     579 
   Size:        339     240
 
Bsu36I CC'TnA_GG

Cuts at:      0     361     579 
   Size:        361     218
 
DrdI GACnn_nn'nnGTC

Cuts at:      0     232     579 
   Size:        232     347
 
EaeI y'GGCC_r

Cuts at:      0     392     518     579 
   Size:        392     126      61

  Fragments arranged by size: 

                 392     126      61
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0      57     520     579 
   Size:         57     463      59

  Fragments arranged by size: 

                 463      59      57
 
EcoO109I rG'GnC_Cy

Cuts at:      0     357     579 
   Size:        357     222
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     507     539     552     579 
   Size:        507      32      13      27

  Fragments arranged by size: 

                 507      32      27      13
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     538     579 
   Size:        538      41
 
MscI TGG'CCA

Cuts at:      0     394     520     579 
   Size:        394     126      59

  Fragments arranged by size: 

                 394     126      59
 
MslI CAynn'nnrTG

Cuts at:      0     139     241     340     579 
   Size:        139     102      99     239

  Fragments arranged by size: 

                 239     139     102      99
 
PpuMI rG'GwC_Cy

Cuts at:      0     357     579 
   Size:        357     222
 
SmlI C'TyrA_G

Cuts at:      0     469     579 
   Size:        469     110
 
StyI C'CwwG_G

Cuts at:      0     488     579 
   Size:        488      91
 
TaqII GACCGAnnnnnnnnn_nn'

Cuts at:      0     407     579 
   Size:        407     172
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     339     387     579 
   Size:        339      48     192

  Fragments arranged by size: 

                 339     192      48
 
XmnI GAAnn'nnTTC

Cuts at:      0     281     313     579 
   Size:        281      32     266

  Fragments arranged by size: 

                 281     266      32
 

 Enzymes that do cut:

     AarI     AccI     AcuI     AvaI     BanI    BanII     BbsI     BglI
     BlpI Bme1580I     BpmI    BpuEI    BsaWI    BsaXI    BseRI  BsiHKAI
 Bsp1286I    BspEI    BspMI    BstXI   Bsu36I     DrdI     EaeI  Eco57MI
 EcoO109I    Hin4I     MmeI     MscI     MslI    PpuMI     SmlI     StyI
    TaqII     XcmI     XmnI

 Enzymes that do not cut: 

    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI     AhdI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     ApoI     AscI
     AseI    AsiSI    AvrII     BaeI    BamHI     BbeI    BbvCI     BcgI
    BciVI     BclI    BfrBI    BglII    BmgBI     BmrI     BmtI     BplI
   Bpu10I     BsaI    BsaAI    BsaBI    BsaHI    BseYI     BsgI    BsiEI
    BsiWI     BsmI    BsmBI    BspHI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstYI  BstZ17I     BtgI
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     EagI     EarI
     EciI  EcoICRI    EcoNI    EcoRI    EcoRV     FalI     FseI     FspI
    FspAI    HaeII   HincII  HindIII     HpaI     KasI     KpnI     MfeI
     MluI   MspA1I     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI
     NotI     NruI     NsiI     NspI     PacI     PasI     PciI    PflMI
     PfoI     PmeI     PmlI     PpiI    PshAI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI     TatI     TstI  Tth111I     XbaI     XhoI     XmaI
     ZraI