Restriction map for
pF1KB3632
(Linear) (Six-base) MAPSORT of: KIBB3632.seq Check: 15 from: 1 to: 1101
KIBB3632.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
Acc65I G'GTAC_C
Cuts at: 0 300 1101
Size: 300 801
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 1101
Size: 61 1040
AfeI AGC'GCT
Cuts at: 0 535 1101
Size: 535 566
AflIII A'CryG_T
Cuts at: 0 172 441 1044 1101
Size: 172 269 603 57
Fragments arranged by size:
603 269 172 57
AlwNI CAG_nnn'CTG
Cuts at: 0 216 279 972 1101
Size: 216 63 693 129
Fragments arranged by size:
693 216 129 63
AvaI C'yCGr_G
Cuts at: 0 146 872 1101
Size: 146 726 229
Fragments arranged by size:
726 229 146
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 284 317 1101
Size: 284 33 784
Fragments arranged by size:
784 284 33
BamHI G'GATC_C
Cuts at: 0 366 1101
Size: 366 735
BanI G'GyrC_C
Cuts at: 0 300 466 628 759 1051 1101
Size: 300 166 162 131 292 50
Fragments arranged by size:
300 292 166 162 131 50
BbeI G_GCGC'C
Cuts at: 0 1055 1101
Size: 1055 46
BbsI GAAGACnn'nnnn_
Cuts at: 0 895 1101
Size: 895 206
BclI T'GATC_A
Cuts at: 0 773 1101
Size: 773 328
BfrBI ATG'CAT
Cuts at: 0 934 1101
Size: 934 167
BglI GCCn_nnn'nGGC
Cuts at: 0 116 1101
Size: 116 985
Bme1580I G_kGCm'C
Cuts at: 0 471 633 770 1101
Size: 471 162 137 331
Fragments arranged by size:
471 331 162 137
BmgBI CAC'GTC
Cuts at: 0 442 1084 1101
Size: 442 642 17
Fragments arranged by size:
642 442 17
BmrI ACTGGGnnnn_n'
Cuts at: 0 289 1101
Size: 289 812
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 826 1101
Size: 826 275
Bpu10I CC'TnA_GC
Cuts at: 0 524 785 1101
Size: 524 261 316
Fragments arranged by size:
524 316 261
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 495 1101
Size: 495 606
BsaI GGTCTCn'nnnn_
Cuts at: 0 543 1101
Size: 543 558
BsaAI yAC'GTr
Cuts at: 0 173 1101
Size: 173 928
BsaHI Gr'CG_yC
Cuts at: 0 1052 1101
Size: 1052 49
BsaWI w'CCGG_w
Cuts at: 0 33 1101
Size: 33 1068
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 823 955 958 1101
Size: 823 132 3 143
Fragments arranged by size:
823 143 132 3
BseYI C'CCAG_C
Cuts at: 0 316 1101
Size: 316 785
Bsp1286I G_dGCh'C
Cuts at: 0 471 633 770 1101
Size: 471 162 137 331
Fragments arranged by size:
471 331 162 137
BspEI T'CCGG_A
Cuts at: 0 33 1101
Size: 33 1068
BsrDI GCAATG_nn'
Cuts at: 0 637 898 998 1101
Size: 637 261 100 103
Fragments arranged by size:
637 261 103 100
BstAPI GCAn_nnn'nTGC
Cuts at: 0 216 1101
Size: 216 885
BstEII G'GTnAC_C
Cuts at: 0 291 1101
Size: 291 810
BstYI r'GATC_y
Cuts at: 0 366 1101
Size: 366 735
Bsu36I CC'TnA_GG
Cuts at: 0 23 185 554 1101
Size: 23 162 369 547
Fragments arranged by size:
547 369 162 23
BtgI C'CryG_G
Cuts at: 0 296 472 518 1014 1101
Size: 296 176 46 496 87
Fragments arranged by size:
496 296 176 87 46
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 976 1101
Size: 976 125
BtsI GCAGTG_nn'
Cuts at: 0 214 697 1101
Size: 214 483 404
Fragments arranged by size:
483 404 214
EaeI y'GGCC_r
Cuts at: 0 719 1101
Size: 719 382
EarI CTCTTCn'nnn_
Cuts at: 0 335 688 1101
Size: 335 353 413
Fragments arranged by size:
413 353 335
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 61 826 1101
Size: 61 765 275
Fragments arranged by size:
765 275 61
EcoNI CCTnn'n_nnAGG
Cuts at: 0 308 1101
Size: 308 793
EcoO109I rG'GnC_Cy
Cuts at: 0 19 313 348 1101
Size: 19 294 35 753
Fragments arranged by size:
753 294 35 19
HaeII r_GCGC'y
Cuts at: 0 537 1055 1101
Size: 537 518 46
Fragments arranged by size:
537 518 46
KasI G'GCGC_C
Cuts at: 0 1051 1101
Size: 1051 50
KpnI G_GTAC'C
Cuts at: 0 304 1101
Size: 304 797
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 278 1101
Size: 278 823
MscI TGG'CCA
Cuts at: 0 721 1101
Size: 721 380
MslI CAynn'nnrTG
Cuts at: 0 289 1101
Size: 289 812
MspA1I CmG'CkG
Cuts at: 0 450 570 645 1101
Size: 450 120 75 456
Fragments arranged by size:
456 450 120 75
NarI GG'CG_CC
Cuts at: 0 1052 1101
Size: 1052 49
NcoI C'CATG_G
Cuts at: 0 296 472 518 1014 1101
Size: 296 176 46 496 87
Fragments arranged by size:
496 296 176 87 46
NsiI A_TGCA'T
Cuts at: 0 936 1101
Size: 936 165
NspI r_CATG'y
Cuts at: 0 108 1048 1101
Size: 108 940 53
Fragments arranged by size:
940 108 53
PciI A'CATG_T
Cuts at: 0 1044 1101
Size: 1044 57
PflMI CCAn_nnn'nTGG
Cuts at: 0 279 536 1101
Size: 279 257 565
Fragments arranged by size:
565 279 257
PmlI CAC'GTG
Cuts at: 0 173 1101
Size: 173 928
PpuMI rG'GwC_Cy
Cuts at: 0 19 313 348 1101
Size: 19 294 35 753
Fragments arranged by size:
753 294 35 19
PstI C_TGCA'G
Cuts at: 0 212 1101
Size: 212 889
PvuII CAG'CTG
Cuts at: 0 570 645 1101
Size: 570 75 456
Fragments arranged by size:
570 456 75
SapI GCTCTTCn'nnn_
Cuts at: 0 335 688 1101
Size: 335 353 413
Fragments arranged by size:
413 353 335
SfcI C'TryA_G
Cuts at: 0 208 1101
Size: 208 893
SfoI GGC'GCC
Cuts at: 0 1053 1101
Size: 1053 48
SmaI CCC'GGG
Cuts at: 0 148 874 1101
Size: 148 726 227
Fragments arranged by size:
726 227 148
SmlI C'TyrA_G
Cuts at: 0 510 1101
Size: 510 591
SphI G_CATG'C
Cuts at: 0 108 1101
Size: 108 993
StuI AGG'CCT
Cuts at: 0 190 755 1101
Size: 190 565 346
Fragments arranged by size:
565 346 190
StyI C'CwwG_G
Cuts at: 0 296 472 518 1014 1101
Size: 296 176 46 496 87
Fragments arranged by size:
496 296 176 87 46
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 605 637 1101
Size: 605 32 464
Fragments arranged by size:
605 464 32
XmaI C'CCGG_G
Cuts at: 0 146 872 1101
Size: 146 726 229
Fragments arranged by size:
726 229 146
Enzymes that do cut:
Acc65I AcuI AfeI AflIII AlwNI AvaI BaeI BamHI
BanI BbeI BbsI BclI BfrBI BglI Bme1580I BmgBI
BmrI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI
BseRI BseYI Bsp1286I BspEI BsrDI BstAPI BstEII BstYI
Bsu36I BtgI BtgZI BtsI EaeI EarI Eco57MI EcoNI
EcoO109I HaeII KasI KpnI MmeI MscI MslI MspA1I
NarI NcoI NsiI NspI PciI PflMI PmlI PpuMI
PstI PvuII SapI SfcI SfoI SmaI SmlI SphI
StuI StyI TstI XmaI
Enzymes that do not cut:
AarI AatII AccI AclI AflII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AseI AsiSI
AvrII BanII BbvCI BcgI BciVI BglII BlpI BmtI
BplI BsaBI BsaXI BsgI BsiEI BsiHKAI BsiWI BsmI
BsmBI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI
BstBI BstXI BstZ17I ClaI CspCI DraI DraIII DrdI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HincII HindIII HpaI MfeI MluI NaeI
NdeI NgoMIV NheI NotI NruI PacI PasI PfoI
PmeI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI ScaI SexAI
SfiI SgfI SgrAI SnaBI SpeI SrfI SspI SwaI
TaqII TatI Tth111I XbaI XcmI XhoI XmnI ZraI