(Linear) (Six-base) MAPSORT of: KIBB3635.seq  Check: 4949  from: 1  to: 534



KIBB3635.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AloI GAACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0      32      64     534 
   Size:         32      32     470

  Fragments arranged by size: 

                 470      32      32
 
AlwNI CAG_nnn'CTG

Cuts at:      0     304     534 
   Size:        304     230
 
BamHI G'GATC_C

Cuts at:      0     323     534 
   Size:        323     211
 
BsaWI w'CCGG_w

Cuts at:      0     185     534 
   Size:        185     349
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0     262     292     534 
   Size:        262      30     242

  Fragments arranged by size: 

                 262     242      30
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     493     534 
   Size:        493      41
 
BseYI C'CCAG_C

Cuts at:      0     399     534 
   Size:        399     135
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     198     534 
   Size:        198     336
 
BspEI T'CCGG_A

Cuts at:      0     185     534 
   Size:        185     349
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     117     534 
   Size:        117     417
 
BstYI r'GATC_y

Cuts at:      0     172     323     534 
   Size:        172     151     211

  Fragments arranged by size: 

                 211     172     151
 
BtgZI GCGATGnnnnnnnnnn'nnnn_

Cuts at:      0     405     534 
   Size:        405     129
 
BtsI GCAGTG_nn'

Cuts at:      0     210     534 
   Size:        210     324
 
ClaI AT'CG_AT

Cuts at:      0     338     534 
   Size:        338     196
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     152     184     534 
   Size:        152      32     350

  Fragments arranged by size: 

                 350     152      32
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0      98     534 
   Size:         98     436
 
PflMI CCAn_nnn'nTGG

Cuts at:      0     367     491     534 
   Size:        367     124      43

  Fragments arranged by size: 

                 367     124      43
 
PfoI T'CCnGG_A

Cuts at:      0     326     534 
   Size:        326     208
 
StyI C'CwwG_G

Cuts at:      0     110     230     534 
   Size:        110     120     304

  Fragments arranged by size: 

                 304     120     110
 

 Enzymes that do cut:

     AloI    AlwNI    BamHI    BsaWI    BsaXI    BseRI    BseYI     BsgI
    BspEI    BstXI    BstYI    BtgZI     BtsI     ClaI    Hin4I     MmeI
    PflMI     PfoI     StyI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     ApaI    ApaLI     ApoI
     AscI     AseI    AsiSI     AvaI    AvrII     BaeI     BanI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI     BplI     BpmI
   Bpu10I    BpuEI     BsaI    BsaAI    BsaBI    BsaHI    BsiEI  BsiHKAI
    BsiWI     BsmI    BsmBI Bsp1286I    BspHI    BspMI    BsrBI    BsrDI
    BsrFI    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII  BstZ17I
   Bsu36I     BtgI    CspCI     DraI   DraIII     DrdI     EaeI     EagI
     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI    EcoRV
     FalI     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI     PmeI     PmlI     PpiI    PpuMI
    PshAI     PsiI   PspOMI    PspXI     PsrI     PstI     PvuI    PvuII
    RsrII     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI
    SexAI     SfcI     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI
    SnaBI     SpeI     SphI     SrfI     SspI     StuI     SwaI    TaqII
     TatI     TstI  Tth111I     XbaI     XcmI     XhoI     XmaI     XmnI
     ZraI