Restriction map for
pF1KB3640
(Linear) (Six-base) MAPSORT of: KIBB3640.seq Check: 8812 from: 1 to: 1257
KIBB3640.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AleI CACnn'nnGTG
Cuts at: 0 961 1257
Size: 961 296
ApoI r'AATT_y
Cuts at: 0 875 1257
Size: 875 382
AvaI C'yCGr_G
Cuts at: 0 416 1257
Size: 416 841
AvrII C'CTAG_G
Cuts at: 0 681 1257
Size: 681 576
BamHI G'GATC_C
Cuts at: 0 7 1257
Size: 7 1250
BanI G'GyrC_C
Cuts at: 0 151 1257
Size: 151 1106
BanII G_rGCy'C
Cuts at: 0 597 1257
Size: 597 660
BbeI G_GCGC'C
Cuts at: 0 155 1257
Size: 155 1102
BbvCI CC'TCA_GC
Cuts at: 0 660 1257
Size: 660 597
BclI T'GATC_A
Cuts at: 0 774 1257
Size: 774 483
BglII A'GATC_T
Cuts at: 0 736 939 1257
Size: 736 203 318
Fragments arranged by size:
736 318 203
Bme1580I G_kGCm'C
Cuts at: 0 417 1257
Size: 417 840
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 581 944 1257
Size: 67 514 363 313
Fragments arranged by size:
514 363 313 67
Bpu10I CC'TnA_GC
Cuts at: 0 660 1257
Size: 660 597
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 1021 1257
Size: 142 879 236
Fragments arranged by size:
879 236 142
BsaI GGTCTCn'nnnn_
Cuts at: 0 827 1257
Size: 827 430
BsaAI yAC'GTr
Cuts at: 0 484 1257
Size: 484 773
BsaBI GATnn'nnATC
Cuts at: 0 995 1257
Size: 995 262
BsaHI Gr'CG_yC
Cuts at: 0 152 1257
Size: 152 1105
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 87 1060 1257
Size: 87 973 197
Fragments arranged by size:
973 197 87
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 521 918 1257
Size: 521 397 339
Fragments arranged by size:
521 397 339
BsmI GAATG_Cn'
Cuts at: 0 810 914 1257
Size: 810 104 343
Fragments arranged by size:
810 343 104
Bsp1286I G_dGCh'C
Cuts at: 0 417 597 1257
Size: 417 180 660
Fragments arranged by size:
660 417 180
BspHI T'CATG_A
Cuts at: 0 450 855 1257
Size: 450 405 402
Fragments arranged by size:
450 405 402
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 60 1257
Size: 60 1197
BstAPI GCAn_nnn'nTGC
Cuts at: 0 168 1257
Size: 168 1089
BstYI r'GATC_y
Cuts at: 0 7 736 939 1257
Size: 7 729 203 318
Fragments arranged by size:
729 318 203 7
BtsI GCAGTG_nn'
Cuts at: 0 803 1257
Size: 803 454
DraI TTT'AAA
Cuts at: 0 519 1257
Size: 519 738
EarI CTCTTCn'nnn_
Cuts at: 0 1004 1257
Size: 1004 253
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 90 1257
Size: 90 1167
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 67 581 944 1257
Size: 67 514 363 313
Fragments arranged by size:
514 363 313 67
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1103 1227 1257
Size: 1103 124 30
Fragments arranged by size:
1103 124 30
EcoO109I rG'GnC_Cy
Cuts at: 0 432 1257
Size: 432 825
EcoRI G'AATT_C
Cuts at: 0 875 1257
Size: 875 382
EcoRV GAT'ATC
Cuts at: 0 1237 1257
Size: 1237 20
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 183 1257
Size: 183 1074
FspI TGC'GCA
Cuts at: 0 1154 1257
Size: 1154 103
HaeII r_GCGC'y
Cuts at: 0 155 238 1257
Size: 155 83 1019
Fragments arranged by size:
1019 155 83
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 824 856 1097 1129 1221 1253 1257
Size: 824 32 241 32 92 32 4
Fragments arranged by size:
824 241 92 32 32 32 4
KasI G'GCGC_C
Cuts at: 0 151 1257
Size: 151 1106
MslI CAynn'nnrTG
Cuts at: 0 397 961 1257
Size: 397 564 296
Fragments arranged by size:
564 397 296
MspA1I CmG'CkG
Cuts at: 0 664 1215 1257
Size: 664 551 42
Fragments arranged by size:
664 551 42
NarI GG'CG_CC
Cuts at: 0 152 1257
Size: 152 1105
NspI r_CATG'y
Cuts at: 0 852 1257
Size: 852 405
PfoI T'CCnGG_A
Cuts at: 0 338 1257
Size: 338 919
PstI C_TGCA'G
Cuts at: 0 641 1257
Size: 641 616
PvuII CAG'CTG
Cuts at: 0 664 1257
Size: 664 593
SfcI C'TryA_G
Cuts at: 0 637 1257
Size: 637 620
SfoI GGC'GCC
Cuts at: 0 153 1257
Size: 153 1104
SmlI C'TyrA_G
Cuts at: 0 157 1000 1257
Size: 157 843 257
Fragments arranged by size:
843 257 157
SphI G_CATG'C
Cuts at: 0 852 1257
Size: 852 405
StuI AGG'CCT
Cuts at: 0 52 1257
Size: 52 1205
StyI C'CwwG_G
Cuts at: 0 681 1257
Size: 681 576
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 795 1257
Size: 795 462
Enzymes that do cut:
AleI ApoI AvaI AvrII BamHI BanI BanII BbeI
BbvCI BclI BglII Bme1580I BpmI Bpu10I BpuEI BsaI
BsaAI BsaBI BsaHI BseRI BsgI BsmI Bsp1286I BspHI
BspMI BstAPI BstYI BtsI DraI EarI EciI Eco57MI
EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I
KasI MslI MspA1I NarI NspI PfoI PstI PvuII
SfcI SfoI SmlI SphI StuI StyI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AlfI AloI AlwNI ApaI ApaLI
AscI AseI AsiSI BaeI BbsI BcgI BciVI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI BsaWI BsaXI
BseYI BsiEI BsiHKAI BsiWI BsmBI BspEI BsrBI BsrDI
BsrFI BsrGI BssHII BssSI BstBI BstEII BstXI BstZ17I
Bsu36I BtgI BtgZI ClaI CspCI DraIII DrdI EaeI
EagI EcoICRI FseI FspAI HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI NaeI NcoI NdeI NgoMIV
NheI NotI NruI NsiI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PvuI RsrII SacI SacII SalI SanDI SapI
SbfI ScaI SexAI SfiI SgfI SgrAI SmaI SnaBI
SpeI SrfI SspI SwaI TaqII TatI TstI Tth111I
XbaI XhoI XmaI XmnI ZraI