(Linear) (Six-base) MAPSORT of: KIBB3641.seq  Check: 9140  from: 1  to: 942



KIBB3641.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

AccI GT'mk_AC

Cuts at:      0     458     860     942 
   Size:        458     402      82

  Fragments arranged by size: 

                 458     402      82
 
AcuI CTGAAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     942 
   Size:        209     733
 
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0      33     942 
   Size:         33     909
 
AlwNI CAG_nnn'CTG

Cuts at:      0     115     630     942 
   Size:        115     515     312

  Fragments arranged by size: 

                 515     312     115
 
ApoI r'AATT_y

Cuts at:      0     814     829     895     942 
   Size:        814      15      66      47

  Fragments arranged by size: 

                 814      66      47      15
 
AvrII C'CTAG_G

Cuts at:      0      30     942 
   Size:         30     912
 
BglI GCCn_nnn'nGGC

Cuts at:      0      32     942 
   Size:         32     910
 
BpmI CTGGAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     425     942 
   Size:        425     517
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     208     942 
   Size:        208     734
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     601     942 
   Size:        601     341
 
BsmI GAATG_Cn'

Cuts at:      0     180     768     942 
   Size:        180     588     174

  Fragments arranged by size: 

                 588     180     174
 
BspHI T'CATG_A

Cuts at:      0     909     942 
   Size:        909      33
 
BspMI ACCTGCnnnn'nnnn_

Cuts at:      0     425     942 
   Size:        425     517
 
BsrDI GCAATG_nn'

Cuts at:      0     768     942 
   Size:        768     174
 
BtgI C'CryG_G

Cuts at:      0     410     657     942 
   Size:        410     247     285

  Fragments arranged by size: 

                 410     285     247
 
BtsI GCAGTG_nn'

Cuts at:      0      17     942 
   Size:         17     925
 
DraI TTT'AAA

Cuts at:      0     207     942 
   Size:        207     735
 
EaeI y'GGCC_r

Cuts at:      0     119     471     942 
   Size:        119     352     471

  Fragments arranged by size: 

                 471     352     119
 
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     209     425     942 
   Size:        209     216     517

  Fragments arranged by size: 

                 517     216     209
 
EcoO109I rG'GnC_Cy

Cuts at:      0     627     942 
   Size:        627     315
 
EcoRI G'AATT_C

Cuts at:      0     814     942 
   Size:        814     128
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     235     942 
   Size:        235     707
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     518     550     553     585     771     803     910     942 
   Size:        518      32       3      32     186      32     107      32

  Fragments arranged by size: 

                 518     186     107      32      32      32      32       3
            
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     257     942 
   Size:        257     685
 
MscI TGG'CCA

Cuts at:      0     121     942 
   Size:        121     821
 
MslI CAynn'nnrTG

Cuts at:      0     254     377     773     824     839     942 
   Size:        254     123     396      51      15     103

  Fragments arranged by size: 

                 396     254     123     103      51      15
 
MspA1I CmG'CkG

Cuts at:      0     687     942 
   Size:        687     255
 
NdeI CA'TA_TG

Cuts at:      0     465     942 
   Size:        465     477
 
PpuMI rG'GwC_Cy

Cuts at:      0     627     942 
   Size:        627     315
 
PshAI GACnn'nnGTC

Cuts at:      0     501     942 
   Size:        501     441
 
PvuII CAG'CTG

Cuts at:      0     687     942 
   Size:        687     255
 
SfcI C'TryA_G

Cuts at:      0     551     942 
   Size:        551     391
 
StyI C'CwwG_G

Cuts at:      0      30     942 
   Size:         30     912
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     390     422     709     741     942 
   Size:        390      32     287      32     201

  Fragments arranged by size: 

                 390     287     201      32      32
 

 Enzymes that do cut:

     AccI     AcuI     AlfI    AlwNI     ApoI    AvrII     BglI     BpmI
     BsaI    BseRI     BsmI    BspHI    BspMI    BsrDI     BtgI     BtsI
     DraI     EaeI  Eco57MI EcoO109I    EcoRI     FalI    Hin4I     MmeI
     MscI     MslI   MspA1I     NdeI    PpuMI    PshAI    PvuII     SfcI
     StyI     TstI

 Enzymes that do not cut: 

     AarI    AatII   Acc65I     AclI     AfeI    AflII   AflIII     AgeI
     AhdI     AleI     AloI     ApaI    ApaLI     AscI     AseI    AsiSI
     AvaI     BaeI    BamHI     BanI    BanII     BbeI     BbsI    BbvCI
     BcgI    BciVI     BclI    BfrBI    BglII     BlpI Bme1580I    BmgBI
     BmrI     BmtI     BplI   Bpu10I    BpuEI    BsaAI    BsaBI    BsaHI
    BsaWI    BsaXI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI    BsmBI
 Bsp1286I    BspEI    BsrBI    BsrFI    BsrGI   BssHII    BssSI   BstAPI
    BstBI   BstEII    BstXI    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI
    CspCI   DraIII     DrdI     EagI     EarI     EciI  EcoICRI    EcoNI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     NaeI     NarI     NcoI   NgoMIV
     NheI     NotI     NruI     NsiI     NspI     PacI     PasI     PciI
    PflMI     PfoI     PmeI     PmlI     PpiI     PsiI   PspOMI    PspXI
     PsrI     PstI     PvuI    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfiI     SfoI     SgfI    SgrAI
     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI     StuI
     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI     XmaI
     XmnI     ZraI