Restriction map for
pF1KB3651
(Linear) (Six-base) MAPSORT of: KIBB3651.seq Check: 3298 from: 1 to: 3012
KIBB3651.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1083 3012
Size: 1083 1929
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1414 2099 2293 2413 2530 3012
Size: 1414 685 194 120 117 482
Fragments arranged by size:
1414 685 482 194 120 117
AflIII A'CryG_T
Cuts at: 0 1806 3012
Size: 1806 1206
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 158 1211 3012
Size: 158 1053 1801
Fragments arranged by size:
1801 1053 158
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 13 45 397 429 3012
Size: 13 32 352 32 2583
Fragments arranged by size:
2583 352 32 32 13
AlwNI CAG_nnn'CTG
Cuts at: 0 117 964 1651 2271 3012
Size: 117 847 687 620 741
Fragments arranged by size:
847 741 687 620 117
ApaI G_GGCC'C
Cuts at: 0 2968 3012
Size: 2968 44
ApaLI G'TGCA_C
Cuts at: 0 1739 1882 3012
Size: 1739 143 1130
Fragments arranged by size:
1739 1130 143
ApoI r'AATT_y
Cuts at: 0 2881 3012
Size: 2881 131
AvaI C'yCGr_G
Cuts at: 0 1664 3012
Size: 1664 1348
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 914 947 3012
Size: 914 33 2065
Fragments arranged by size:
2065 914 33
BanI G'GyrC_C
Cuts at: 0 567 3012
Size: 567 2445
BanII G_rGCy'C
Cuts at: 0 364 2968 3012
Size: 364 2604 44
Fragments arranged by size:
2604 364 44
BbsI GAAGACnn'nnnn_
Cuts at: 0 1427 2642 3012
Size: 1427 1215 370
Fragments arranged by size:
1427 1215 370
BbvCI CC'TCA_GC
Cuts at: 0 645 1343 3012
Size: 645 698 1669
Fragments arranged by size:
1669 698 645
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2827 2861 3012
Size: 2827 34 151
Fragments arranged by size:
2827 151 34
BglI GCCn_nnn'nGGC
Cuts at: 0 1012 1705 3012
Size: 1012 693 1307
Fragments arranged by size:
1307 1012 693
BglII A'GATC_T
Cuts at: 0 2978 3012
Size: 2978 34
BlpI GC'TnA_GC
Cuts at: 0 1101 3012
Size: 1101 1911
Bme1580I G_kGCm'C
Cuts at: 0 1743 1756 1886 2968 3012
Size: 1743 13 130 1082 44
Fragments arranged by size:
1743 1082 130 44 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 409 1380 3012
Size: 409 971 1632
Fragments arranged by size:
1632 971 409
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 39 3012
Size: 39 2973
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 608 677 1147 1246 1976 3012
Size: 157 451 69 470 99 730 1036
Fragments arranged by size:
1036 730 470 451 157 99 69
Bpu10I CC'TnA_GC
Cuts at: 0 51 645 1343 1469 3012
Size: 51 594 698 126 1543
Fragments arranged by size:
1543 698 594 126 51
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 635 2959 2986 3012
Size: 635 2324 27 26
Fragments arranged by size:
2324 635 27 26
BsaI GGTCTCn'nnnn_
Cuts at: 0 3001 3012
Size: 3001 11
BsaBI GATnn'nnATC
Cuts at: 0 605 1198 3012
Size: 605 593 1814
Fragments arranged by size:
1814 605 593
BsaWI w'CCGG_w
Cuts at: 0 2766 3012
Size: 2766 246
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 397 427 1770 1800 3012
Size: 397 30 1343 30 1212
Fragments arranged by size:
1343 1212 397 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1701 1824 1951 1954 2574 2842 3012
Size: 1701 123 127 3 620 268 170
Fragments arranged by size:
1701 620 268 170 127 123 3
BseYI C'CCAG_C
Cuts at: 0 194 332 462 1081 1235 1391 1553 2716
Size: 194 138 130 619 154 156 162 1163
Cuts at: 2716 2809 2961 3012
Size: 93 152 51
Fragments arranged by size:
1163 619 194 162 156 154 152 138
130 93 51
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 130 955 1019 1705 1813 3012
Size: 130 825 64 686 108 1199
Fragments arranged by size:
1199 825 686 130 108 64
BsiEI CG_ry'CG
Cuts at: 0 329 3012
Size: 329 2683
BsiHKAI G_wGCw'C
Cuts at: 0 364 1743 1886 3012
Size: 364 1379 143 1126
Fragments arranged by size:
1379 1126 364 143
BsmI GAATG_Cn'
Cuts at: 0 2073 3012
Size: 2073 939
Bsp1286I G_dGCh'C
Cuts at: 0 364 1743 1756 1886 2968 3012
Size: 364 1379 13 130 1082 44
Fragments arranged by size:
1379 1082 364 130 44 13
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 468 1083 1362 3012
Size: 468 615 279 1650
Fragments arranged by size:
1650 615 468 279
BsrDI GCAATG_nn'
Cuts at: 0 2591 3012
Size: 2591 421
BsrFI r'CCGG_y
Cuts at: 0 1226 3012
Size: 1226 1786
BstAPI GCAn_nnn'nTGC
Cuts at: 0 117 126 567 942 997 1881 3012
Size: 117 9 441 375 55 884 1131
Fragments arranged by size:
1131 884 441 375 117 55 9
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1735 3012
Size: 1735 1277
BstYI r'GATC_y
Cuts at: 0 1118 1154 2978 3012
Size: 1118 36 1824 34
Fragments arranged by size:
1824 1118 36 34
Bsu36I CC'TnA_GG
Cuts at: 0 888 3012
Size: 888 2124
BtgI C'CryG_G
Cuts at: 0 833 2138 3012
Size: 833 1305 874
Fragments arranged by size:
1305 874 833
BtsI GCAGTG_nn'
Cuts at: 0 113 124 816 1334 1741 2091 3012
Size: 113 11 692 518 407 350 921
Fragments arranged by size:
921 692 518 407 350 113 11
DraIII CAC_nnn'GTG
Cuts at: 0 865 3012
Size: 865 2147
EaeI y'GGCC_r
Cuts at: 0 314 326 454 1032 3012
Size: 314 12 128 578 1980
Fragments arranged by size:
1980 578 314 128 12
EagI C'GGCC_G
Cuts at: 0 326 3012
Size: 326 2686
EarI CTCTTCn'nnn_
Cuts at: 0 1454 2020 2506 3012
Size: 1454 566 486 506
Fragments arranged by size:
1454 566 506 486
EcoICRI GAG'CTC
Cuts at: 0 362 3012
Size: 362 2650
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 157 608 677 1147 1246 1414 1976 2099
Size: 157 451 69 470 99 168 562 123
Cuts at: 2099 2293 2413 2530 3012
Size: 194 120 117 482
Fragments arranged by size:
562 482 470 451 194 168 157 123
120 117 99 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 475 721 887 1526 3012
Size: 475 246 166 639 1486
Fragments arranged by size:
1486 639 475 246 166
EcoO109I rG'GnC_Cy
Cuts at: 0 655 738 1356 1374 1854 2910 2965 3012
Size: 655 83 618 18 480 1056 55 47
Fragments arranged by size:
1056 655 618 480 83 55 47 18
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2953 3012
Size: 2953 59
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 556 588 1770 1802 1937 1939 1969 1971
Size: 556 32 1182 32 135 2 30 2
Cuts at: 1971 2310 2314 2342 2344 2346 2376 3012
Size: 339 4 28 2 2 30 636
Fragments arranged by size:
1182 636 556 339 135 32 32 30
30 28 4 2 2 2 2
MfeI C'AATT_G
Cuts at: 0 230 3012
Size: 230 2782
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 478 2332 2710 2797 3012
Size: 478 1854 378 87 215
Fragments arranged by size:
1854 478 378 215 87
MscI TGG'CCA
Cuts at: 0 316 1034 3012
Size: 316 718 1978
Fragments arranged by size:
1978 718 316
MslI CAynn'nnrTG
Cuts at: 0 529 599 3012
Size: 529 70 2413
Fragments arranged by size:
2413 529 70
MspA1I CmG'CkG
Cuts at: 0 132 198 462 1549 1641 1648 3012
Size: 132 66 264 1087 92 7 1364
Fragments arranged by size:
1364 1087 264 132 92 66 7
NcoI C'CATG_G
Cuts at: 0 833 2138 3012
Size: 833 1305 874
Fragments arranged by size:
1305 874 833
NspI r_CATG'y
Cuts at: 0 1810 3012
Size: 1810 1202
PasI CC'CwG_GG
Cuts at: 0 492 826 1771 3012
Size: 492 334 945 1241
Fragments arranged by size:
1241 945 492 334
PciI A'CATG_T
Cuts at: 0 1806 3012
Size: 1806 1206
PflMI CCAn_nnn'nTGG
Cuts at: 0 1410 1908 2816 3012
Size: 1410 498 908 196
Fragments arranged by size:
1410 908 498 196
PfoI T'CCnGG_A
Cuts at: 0 585 693 2228 3012
Size: 585 108 1535 784
Fragments arranged by size:
1535 784 585 108
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 397 429 3012
Size: 397 32 2583
Fragments arranged by size:
2583 397 32
PpuMI rG'GwC_Cy
Cuts at: 0 655 1356 1374 1854 3012
Size: 655 701 18 480 1158
Fragments arranged by size:
1158 701 655 480 18
PspOMI G'GGCC_C
Cuts at: 0 2964 3012
Size: 2964 48
PstI C_TGCA'G
Cuts at: 0 131 555 951 1748 3012
Size: 131 424 396 797 1264
Fragments arranged by size:
1264 797 424 396 131
PvuII CAG'CTG
Cuts at: 0 198 462 1549 1641 1648 3012
Size: 198 264 1087 92 7 1364
Fragments arranged by size:
1364 1087 264 198 92 7
SacI G_AGCT'C
Cuts at: 0 364 3012
Size: 364 2648
SapI GCTCTTCn'nnn_
Cuts at: 0 2020 3012
Size: 2020 992
ScaI AGT'ACT
Cuts at: 0 2404 3012
Size: 2404 608
SfcI C'TryA_G
Cuts at: 0 127 551 947 1502 1744 2293 2490 3012
Size: 127 424 396 555 242 549 197 522
Fragments arranged by size:
555 549 522 424 396 242 197 127
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1705 3012
Size: 1705 1307
SmaI CCC'GGG
Cuts at: 0 1666 3012
Size: 1666 1346
SmlI C'TyrA_G
Cuts at: 0 650 2974 3001 3012
Size: 650 2324 27 11
Fragments arranged by size:
2324 650 27 11
SpeI A'CTAG_T
Cuts at: 0 2091 3012
Size: 2091 921
StuI AGG'CCT
Cuts at: 0 301 643 727 3012
Size: 301 342 84 2285
Fragments arranged by size:
2285 342 301 84
StyI C'CwwG_G
Cuts at: 0 833 1982 2138 2856 3012
Size: 833 1149 156 718 156
Fragments arranged by size:
1149 833 718 156 156
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 310 886 2146 2218 3012
Size: 310 576 1260 72 794
Fragments arranged by size:
1260 794 576 310 72
TatI w'GTAC_w
Cuts at: 0 2402 3012
Size: 2402 610
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1216 1248 1340 1372 1556 1588 3012
Size: 1216 32 92 32 184 32 1424
Fragments arranged by size:
1424 1216 184 92 32 32 32
XbaI T'CTAG_A
Cuts at: 0 2146 3012
Size: 2146 866
XmaI C'CCGG_G
Cuts at: 0 1664 3012
Size: 1664 1348
Enzymes that do cut:
AarI AcuI AflIII AlfI AloI AlwNI ApaI ApaLI
ApoI AvaI BaeI BanI BanII BbsI BbvCI BcgI
BglI BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I
BpuEI BsaI BsaBI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrDI BsrFI BstAPI
BstXI BstYI Bsu36I BtgI BtsI DraIII EaeI EagI
EarI EcoICRI Eco57MI EcoNI EcoO109I FalI Hin4I MfeI
MmeI MscI MslI MspA1I NcoI NspI PasI PciI
PflMI PfoI PpiI PpuMI PspOMI PstI PvuII SacI
SapI ScaI SfcI SfiI SmaI SmlI SpeI StuI
StyI TaqII TatI TstI XbaI XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AleI AscI AseI AsiSI AvrII BamHI BbeI BciVI
BclI BfrBI BmgBI BmtI BsaAI BsaHI BsiWI BsmBI
BspEI BspHI BsrBI BsrGI BssHII BssSI BstBI BstEII
BstZ17I BtgZI ClaI CspCI DraI DrdI EciI EcoRI
EcoRV FseI FspI FspAI HaeII HincII HindIII HpaI
KasI KpnI MluI NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PmeI PmlI PshAI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SexAI SfoI SgfI SgrAI SnaBI SphI SrfI SspI
SwaI Tth111I XcmI XhoI XmnI ZraI