Restriction map for
pF1KB3652
(Linear) (Six-base) MAPSORT of: KIBB3652.seq Check: 8475 from: 1 to: 2304
KIBB3652.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AccI GT'mk_AC
Cuts at: 0 314 2304
Size: 314 1990
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1871 2024 2180 2296 2304
Size: 1871 153 156 116 8
Fragments arranged by size:
1871 156 153 116 8
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 209 2304
Size: 209 2095
AlwNI CAG_nnn'CTG
Cuts at: 0 736 2304
Size: 736 1568
ApaI G_GGCC'C
Cuts at: 0 1774 2304
Size: 1774 530
ApaLI G'TGCA_C
Cuts at: 0 712 2304
Size: 712 1592
ApoI r'AATT_y
Cuts at: 0 1126 1620 2304
Size: 1126 494 684
Fragments arranged by size:
1126 684 494
AvaI C'yCGr_G
Cuts at: 0 2128 2304
Size: 2128 176
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 287 320 2304
Size: 287 33 1984
Fragments arranged by size:
1984 287 33
BanI G'GyrC_C
Cuts at: 0 123 2304
Size: 123 2181
BanII G_rGCy'C
Cuts at: 0 911 1774 1849 2304
Size: 911 863 75 455
Fragments arranged by size:
911 863 455 75
BbsI GAAGACnn'nnnn_
Cuts at: 0 1336 1411 1958 2304
Size: 1336 75 547 346
Fragments arranged by size:
1336 547 346 75
BbvCI CC'TCA_GC
Cuts at: 0 2175 2304
Size: 2175 129
BglI GCCn_nnn'nGGC
Cuts at: 0 1720 2155 2304
Size: 1720 435 149
Fragments arranged by size:
1720 435 149
BglII A'GATC_T
Cuts at: 0 468 2304
Size: 468 1836
Bme1580I G_kGCm'C
Cuts at: 0 716 1285 1774 2304
Size: 716 569 489 530
Fragments arranged by size:
716 569 530 489
BmgBI CAC'GTC
Cuts at: 0 699 1752 2304
Size: 699 1053 552
Fragments arranged by size:
1053 699 552
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1828 1880 1966 1997 2207 2304
Size: 1828 52 86 31 210 97
Fragments arranged by size:
1828 210 97 86 52 31
Bpu10I CC'TnA_GC
Cuts at: 0 2175 2304
Size: 2175 129
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 126 2304
Size: 126 2178
BsaI GGTCTCn'nnnn_
Cuts at: 0 488 747 1076 2225 2304
Size: 488 259 329 1149 79
Fragments arranged by size:
1149 488 329 259 79
BsaAI yAC'GTr
Cuts at: 0 1440 2304
Size: 1440 864
BsaWI w'CCGG_w
Cuts at: 0 896 1074 1468 2304
Size: 896 178 394 836
Fragments arranged by size:
896 836 394 178
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 602 632 914 944 1691 1721 2304
Size: 602 30 282 30 747 30 583
Fragments arranged by size:
747 602 583 282 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 949 1825 2089 2200 2304
Size: 949 876 264 111 104
Fragments arranged by size:
949 876 264 111 104
BseYI C'CCAG_C
Cuts at: 0 318 726 1927 2145 2304
Size: 318 408 1201 218 159
Fragments arranged by size:
1201 408 318 218 159
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 671 1022 2304
Size: 671 351 1282
Fragments arranged by size:
1282 671 351
BsiEI CG_ry'CG
Cuts at: 0 78 2304
Size: 78 2226
BsiHKAI G_wGCw'C
Cuts at: 0 716 1765 2304
Size: 716 1049 539
Fragments arranged by size:
1049 716 539
BsmI GAATG_Cn'
Cuts at: 0 824 2304
Size: 824 1480
Bsp1286I G_dGCh'C
Cuts at: 0 716 911 1285 1765 1774 1849 2304
Size: 716 195 374 480 9 75 455
Fragments arranged by size:
716 480 455 374 195 75 9
BspEI T'CCGG_A
Cuts at: 0 896 2304
Size: 896 1408
BspHI T'CATG_A
Cuts at: 0 810 2304
Size: 810 1494
BsrDI GCAATG_nn'
Cuts at: 0 755 2304
Size: 755 1549
BsrFI r'CCGG_y
Cuts at: 0 655 2304
Size: 655 1649
BssSI C'ACGA_G
Cuts at: 0 14 910 2304
Size: 14 896 1394
Fragments arranged by size:
1394 896 14
BstAPI GCAn_nnn'nTGC
Cuts at: 0 682 736 2304
Size: 682 54 1568
Fragments arranged by size:
1568 682 54
BstBI TT'CG_AA
Cuts at: 0 987 2304
Size: 987 1317
BstEII G'GTnAC_C
Cuts at: 0 27 2304
Size: 27 2277
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1944 2304
Size: 1944 360
BstYI r'GATC_y
Cuts at: 0 394 468 977 1607 2304
Size: 394 74 509 630 697
Fragments arranged by size:
697 630 509 394 74
BtgI C'CryG_G
Cuts at: 0 819 903 1766 2198 2304
Size: 819 84 863 432 106
Fragments arranged by size:
863 819 432 106 84
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1238 2228 2304
Size: 1238 990 76
Fragments arranged by size:
1238 990 76
ClaI AT'CG_AT
Cuts at: 0 1185 2304
Size: 1185 1119
DrdI GACnn_nn'nnGTC
Cuts at: 0 1021 2304
Size: 1021 1283
EaeI y'GGCC_r
Cuts at: 0 995 1350 2304
Size: 995 355 954
Fragments arranged by size:
995 954 355
EarI CTCTTCn'nnn_
Cuts at: 0 160 833 1847 2156 2275 2287 2304
Size: 160 673 1014 309 119 12 17
Fragments arranged by size:
1014 673 309 160 119 17 12
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1828 1871 1880 1966 1997 2024 2180 2207
Size: 1828 43 9 86 31 27 156 27
Cuts at: 2207 2296 2304
Size: 89 8
Fragments arranged by size:
1828 156 89 86 43 31 27 27
9 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 136 1693 2304
Size: 136 1557 611
Fragments arranged by size:
1557 611 136
EcoO109I rG'GnC_Cy
Cuts at: 0 871 1771 2109 2304
Size: 871 900 338 195
Fragments arranged by size:
900 871 338 195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 212 2304
Size: 212 2092
HaeII r_GCGC'y
Cuts at: 0 787 2304
Size: 787 1517
HindIII A'AGCT_T
Cuts at: 0 1364 2304
Size: 1364 940
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 900 2304
Size: 900 1404
MscI TGG'CCA
Cuts at: 0 997 2304
Size: 997 1307
MslI CAynn'nnrTG
Cuts at: 0 1144 2304
Size: 1144 1160
MspA1I CmG'CkG
Cuts at: 0 1323 2179 2200 2304
Size: 1323 856 21 104
Fragments arranged by size:
1323 856 104 21
NaeI GCC'GGC
Cuts at: 0 657 2304
Size: 657 1647
NcoI C'CATG_G
Cuts at: 0 819 903 2304
Size: 819 84 1401
Fragments arranged by size:
1401 819 84
NgoMIV G'CCGG_C
Cuts at: 0 655 2304
Size: 655 1649
PasI CC'CwG_GG
Cuts at: 0 1066 2304
Size: 1066 1238
PfoI T'CCnGG_A
Cuts at: 0 1589 2304
Size: 1589 715
PmlI CAC'GTG
Cuts at: 0 1440 2304
Size: 1440 864
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 524 556 2304
Size: 524 32 1748
Fragments arranged by size:
1748 524 32
PspOMI G'GGCC_C
Cuts at: 0 1770 2304
Size: 1770 534
PstI C_TGCA'G
Cuts at: 0 1457 2304
Size: 1457 847
PvuII CAG'CTG
Cuts at: 0 2179 2304
Size: 2179 125
SacII CC_GC'GG
Cuts at: 0 2201 2304
Size: 2201 103
SapI GCTCTTCn'nnn_
Cuts at: 0 2275 2304
Size: 2275 29
ScaI AGT'ACT
Cuts at: 0 103 2304
Size: 103 2201
SfcI C'TryA_G
Cuts at: 0 1164 1453 2304
Size: 1164 289 851
Fragments arranged by size:
1164 851 289
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1720 2304
Size: 1720 584
SmlI C'TyrA_G
Cuts at: 0 105 2304
Size: 105 2199
StuI AGG'CCT
Cuts at: 0 260 940 2304
Size: 260 680 1364
Fragments arranged by size:
1364 680 260
StyI C'CwwG_G
Cuts at: 0 792 819 903 1718 2304
Size: 792 27 84 815 586
Fragments arranged by size:
815 792 586 84 27
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 92 467 2304
Size: 92 375 1837
Fragments arranged by size:
1837 375 92
TatI w'GTAC_w
Cuts at: 0 101 1043 2304
Size: 101 942 1261
Fragments arranged by size:
1261 942 101
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 688 720 1691 1723 2039 2071 2304
Size: 688 32 971 32 316 32 233
Fragments arranged by size:
971 688 316 233 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 261 1663 2304
Size: 261 1402 641
Fragments arranged by size:
1402 641 261
XmnI GAAnn'nnTTC
Cuts at: 0 2254 2304
Size: 2254 50
Enzymes that do cut:
AccI AcuI AlfI AlwNI ApaI ApaLI ApoI AvaI
BaeI BanI BanII BbsI BbvCI BglI BglII Bme1580I
BmgBI BpmI Bpu10I BpuEI BsaI BsaAI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BsrDI BsrFI BssSI BstAPI BstBI BstEII BstXI
BstYI BtgI BtgZI ClaI DrdI EaeI EarI Eco57MI
EcoNI EcoO109I FalI HaeII HindIII MmeI MscI MslI
MspA1I NaeI NcoI NgoMIV PasI PfoI PmlI PpiI
PspOMI PstI PvuII SacII SapI ScaI SfcI SfiI
SmlI StuI StyI TaqII TatI TstI XcmI XmnI
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AflIII AgeI
AhdI AleI AloI AscI AseI AsiSI AvrII BamHI
BbeI BcgI BciVI BclI BfrBI BlpI BmrI BmtI
BplI BsaBI BsaHI BsiWI BsmBI BspMI BsrBI BsrGI
BssHII BstZ17I Bsu36I BtsI CspCI DraI DraIII EagI
EciI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I
HincII HpaI KasI KpnI MfeI MluI NarI NdeI
NheI NotI NruI NsiI NspI PacI PciI PflMI
PmeI PpuMI PshAI PsiI PspXI PsrI PvuI RsrII
SacI SalI SanDI SbfI SexAI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI Tth111I
XbaI XhoI XmaI ZraI