Restriction map for
pF1KB3674
(Linear) (Six-base) MAPSORT of: KIBB3674.seq Check: 7670 from: 1 to: 1545
KIBB3674.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 98 1545
Size: 98 1447
AfeI AGC'GCT
Cuts at: 0 458 1545
Size: 458 1087
AflIII A'CryG_T
Cuts at: 0 1270 1545
Size: 1270 275
AhdI GACnn_n'nnGTC
Cuts at: 0 936 1545
Size: 936 609
AlwNI CAG_nnn'CTG
Cuts at: 0 199 369 489 788 1545
Size: 199 170 120 299 757
Fragments arranged by size:
757 299 199 170 120
ApoI r'AATT_y
Cuts at: 0 1348 1545
Size: 1348 197
BanI G'GyrC_C
Cuts at: 0 255 1545
Size: 255 1290
BanII G_rGCy'C
Cuts at: 0 888 1545
Size: 888 657
BbsI GAAGACnn'nnnn_
Cuts at: 0 471 501 1545
Size: 471 30 1044
Fragments arranged by size:
1044 471 30
BclI T'GATC_A
Cuts at: 0 1019 1545
Size: 1019 526
BlpI GC'TnA_GC
Cuts at: 0 1505 1545
Size: 1505 40
Bme1580I G_kGCm'C
Cuts at: 0 1039 1545
Size: 1039 506
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 475 1465 1545
Size: 475 990 80
Fragments arranged by size:
990 475 80
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1545
Size: 872 673
Bpu10I CC'TnA_GC
Cuts at: 0 296 1079 1217 1227 1545
Size: 296 783 138 10 318
Fragments arranged by size:
783 318 296 138 10
BsaI GGTCTCn'nnnn_
Cuts at: 0 498 1545
Size: 498 1047
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 71 74 650 1520 1545
Size: 71 3 576 870 25
Fragments arranged by size:
870 576 71 25 3
BseYI C'CCAG_C
Cuts at: 0 291 1545
Size: 291 1254
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 76 1545
Size: 76 1469
BsmBI CGTCTCn'nnnn_
Cuts at: 0 660 1545
Size: 660 885
Bsp1286I G_dGCh'C
Cuts at: 0 888 1039 1545
Size: 888 151 506
Fragments arranged by size:
888 506 151
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 28 98 1545
Size: 28 70 1447
Fragments arranged by size:
1447 70 28
BsrDI GCAATG_nn'
Cuts at: 0 927 1061 1545
Size: 927 134 484
Fragments arranged by size:
927 484 134
BsrFI r'CCGG_y
Cuts at: 0 252 1545
Size: 252 1293
BsrGI T'GTAC_A
Cuts at: 0 144 153 1545
Size: 144 9 1392
Fragments arranged by size:
1392 144 9
BstAPI GCAn_nnn'nTGC
Cuts at: 0 199 1545
Size: 199 1346
BstEII G'GTnAC_C
Cuts at: 0 40 1545
Size: 40 1505
BstXI CCAn_nnnn'nTGG
Cuts at: 0 736 1545
Size: 736 809
BstYI r'GATC_y
Cuts at: 0 432 1026 1545
Size: 432 594 519
Fragments arranged by size:
594 519 432
EaeI y'GGCC_r
Cuts at: 0 818 1545
Size: 818 727
EarI CTCTTCn'nnn_
Cuts at: 0 174 1124 1302 1545
Size: 174 950 178 243
Fragments arranged by size:
950 243 178 174
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 872 1545
Size: 872 673
EcoO109I rG'GnC_Cy
Cuts at: 0 1099 1545
Size: 1099 446
EcoRV GAT'ATC
Cuts at: 0 1264 1545
Size: 1264 281
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 712 1242 1545
Size: 712 530 303
Fragments arranged by size:
712 530 303
HaeII r_GCGC'y
Cuts at: 0 460 1079 1545
Size: 460 619 466
Fragments arranged by size:
619 466 460
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 922 954 1011 1043 1545
Size: 922 32 57 32 502
Fragments arranged by size:
922 502 57 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 600 992 1410 1545
Size: 600 392 418 135
Fragments arranged by size:
600 418 392 135
MscI TGG'CCA
Cuts at: 0 820 1545
Size: 820 725
MslI CAynn'nnrTG
Cuts at: 0 548 1273 1275 1545
Size: 548 725 2 270
Fragments arranged by size:
725 548 270 2
MspA1I CmG'CkG
Cuts at: 0 142 178 196 205 280 316 369 736
Size: 142 36 18 9 75 36 53 367
Cuts at: 736 1545
Size: 809
Fragments arranged by size:
809 367 142 75 53 36 36 18
9
NdeI CA'TA_TG
Cuts at: 0 851 1545
Size: 851 694
NspI r_CATG'y
Cuts at: 0 1274 1545
Size: 1274 271
PasI CC'CwG_GG
Cuts at: 0 223 1545
Size: 223 1322
PciI A'CATG_T
Cuts at: 0 1270 1545
Size: 1270 275
PflMI CCAn_nnn'nTGG
Cuts at: 0 442 489 1545
Size: 442 47 1056
Fragments arranged by size:
1056 442 47
PfoI T'CCnGG_A
Cuts at: 0 270 512 1545
Size: 270 242 1033
Fragments arranged by size:
1033 270 242
PpuMI rG'GwC_Cy
Cuts at: 0 1099 1545
Size: 1099 446
PstI C_TGCA'G
Cuts at: 0 30 204 210 285 1545
Size: 30 174 6 75 1260
Fragments arranged by size:
1260 174 75 30 6
PvuII CAG'CTG
Cuts at: 0 178 205 280 316 369 736 1545
Size: 178 27 75 36 53 367 809
Fragments arranged by size:
809 367 178 75 53 36 27
SfcI C'TryA_G
Cuts at: 0 26 200 206 281 320 1545
Size: 26 174 6 75 39 1225
Fragments arranged by size:
1225 174 75 39 26 6
SspI AAT'ATT
Cuts at: 0 980 1545
Size: 980 565
TatI w'GTAC_w
Cuts at: 0 144 153 552 1125 1545
Size: 144 9 399 573 420
Fragments arranged by size:
573 420 399 144 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1053 1085 1427 1459 1545
Size: 1053 32 342 32 86
Fragments arranged by size:
1053 342 86 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1008 1545
Size: 1008 537
XmnI GAAnn'nnTTC
Cuts at: 0 1163 1403 1545
Size: 1163 240 142
Fragments arranged by size:
1163 240 142
Enzymes that do cut:
AarI AfeI AflIII AhdI AlwNI ApoI BanI BanII
BbsI BclI BlpI Bme1580I BplI BpmI Bpu10I BsaI
BseRI BseYI BsgI BsmBI Bsp1286I BspMI BsrDI BsrFI
BsrGI BstAPI BstEII BstXI BstYI EaeI EarI Eco57MI
EcoO109I EcoRV FalI HaeII Hin4I MmeI MscI MslI
MspA1I NdeI NspI PasI PciI PflMI PfoI PpuMI
PstI PvuII SfcI SspI TatI TstI XcmI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AleI
AlfI AloI ApaI ApaLI AscI AseI AsiSI AvaI
AvrII BaeI BamHI BbeI BbvCI BcgI BciVI BfrBI
BglI BglII BmgBI BmrI BmtI BpuEI BsaAI BsaBI
BsaHI BsaWI BsaXI BsiEI BsiHKAI BsiWI BsmI BspEI
BspHI BsrBI BssHII BssSI BstBI BstZ17I Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EagI
EciI EcoICRI EcoNI EcoRI FseI FspI FspAI HincII
HindIII HpaI KasI KpnI MfeI MluI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SapI SbfI ScaI
SexAI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SrfI StuI StyI SwaI TaqII Tth111I
XbaI XhoI XmaI ZraI