Restriction map for
pF1KB3681
(Linear) (Six-base) MAPSORT of: KIBB3681.seq Check: 1376 from: 1 to: 2685
KIBB3681.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 472 1537 1888 2487 2685
Size: 472 1065 351 599 198
Fragments arranged by size:
1065 599 472 351 198
AfeI AGC'GCT
Cuts at: 0 1795 2685
Size: 1795 890
AflII C'TTAA_G
Cuts at: 0 1594 2685
Size: 1594 1091
AflIII A'CryG_T
Cuts at: 0 2570 2685
Size: 2570 115
AgeI A'CCGG_T
Cuts at: 0 2622 2685
Size: 2622 63
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1493 2299 2685
Size: 1493 806 386
Fragments arranged by size:
1493 806 386
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 1215 1216 1247 1248 2685
Size: 1177 32 6 1 31 1 1437
Fragments arranged by size:
1437 1177 32 31 6 1 1
AlwNI CAG_nnn'CTG
Cuts at: 0 694 929 1884 2254 2685
Size: 694 235 955 370 431
Fragments arranged by size:
955 694 431 370 235
ApaI G_GGCC'C
Cuts at: 0 264 469 815 914 941 2109 2588 2685
Size: 264 205 346 99 27 1168 479 97
Fragments arranged by size:
1168 479 346 264 205 99 97 27
ApaLI G'TGCA_C
Cuts at: 0 2145 2685
Size: 2145 540
AvaI C'yCGr_G
Cuts at: 0 18 575 769 815 1801 2434 2580 2672
Size: 18 557 194 46 986 633 146 92
Cuts at: 2672 2685
Size: 13
Fragments arranged by size:
986 633 557 194 146 92 46 18
13
BamHI G'GATC_C
Cuts at: 0 168 326 2685
Size: 168 158 2359
Fragments arranged by size:
2359 168 158
BanI G'GyrC_C
Cuts at: 0 25 70 459 480 831 1971 2051 2175
Size: 25 45 389 21 351 1140 80 124
Cuts at: 2175 2619 2685
Size: 444 66
Fragments arranged by size:
1140 444 389 351 124 80 66 45
25 21
BanII G_rGCy'C
Cuts at: 0 18 44 142 264 292 469 536 815
Size: 18 26 98 122 28 177 67 279
Cuts at: 815 914 941 1142 1574 1750 1808 2027 2109
Size: 99 27 201 432 176 58 219 82
Cuts at: 2109 2376 2588 2685
Size: 267 212 97
Fragments arranged by size:
432 279 267 219 212 201 177 176
122 99 98 97 82 67 58 28
27 26 18
BbeI G_GCGC'C
Cuts at: 0 29 74 2685
Size: 29 45 2611
Fragments arranged by size:
2611 45 29
BbsI GAAGACnn'nnnn_
Cuts at: 0 1934 2460 2685
Size: 1934 526 225
Fragments arranged by size:
1934 526 225
BbvCI CC'TCA_GC
Cuts at: 0 2491 2685
Size: 2491 194
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 1454 1488 2685
Size: 1454 34 1197
Fragments arranged by size:
1454 1197 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 642 2685
Size: 642 2043
BfrBI ATG'CAT
Cuts at: 0 3 1506 2685
Size: 3 1503 1179
Fragments arranged by size:
1503 1179 3
BglI GCCn_nnn'nGGC
Cuts at: 0 2251 2558 2685
Size: 2251 307 127
Fragments arranged by size:
2251 307 127
Bme1580I G_kGCm'C
Cuts at: 0 264 462 469 485 761 815 836 914
Size: 264 198 7 16 276 54 21 78
Cuts at: 914 941 1073 1203 1673 1713 1974 2109 2149
Size: 27 132 130 470 40 261 135 40
Cuts at: 2149 2588 2685
Size: 439 97
Fragments arranged by size:
470 439 276 264 261 198 135 132
130 97 78 54 40 40 27 21
16 7
BmrI ACTGGGnnnn_n'
Cuts at: 0 168 236 611 1060 1848 2685
Size: 168 68 375 449 788 837
Fragments arranged by size:
837 788 449 375 168 68
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 197 304 550 1036 1363 1465 1792
Size: 125 72 107 246 486 327 102 327
Cuts at: 1792 1906 2254 2685
Size: 114 348 431
Fragments arranged by size:
486 431 348 327 327 246 125 114
107 102 72
Bpu10I CC'TnA_GC
Cuts at: 0 141 2491 2685
Size: 141 2350 194
Fragments arranged by size:
2350 194 141
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1366 1607 2685
Size: 1366 241 1078
Fragments arranged by size:
1366 1078 241
BsaI GGTCTCn'nnnn_
Cuts at: 0 880 2685
Size: 880 1805
BsaHI Gr'CG_yC
Cuts at: 0 26 71 2685
Size: 26 45 2614
Fragments arranged by size:
2614 45 26
BsaWI w'CCGG_w
Cuts at: 0 323 2622 2685
Size: 323 2299 63
Fragments arranged by size:
2299 323 63
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1261 1291 2116 2146 2685
Size: 1261 30 825 30 539
Fragments arranged by size:
1261 825 539 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 898 1033 1036 1048 1051 1054 1558 1837
Size: 898 135 3 12 3 3 504 279
Cuts at: 1837 1840 2629 2685
Size: 3 789 56
Fragments arranged by size:
898 789 504 279 135 56 12 3
3 3 3
BseYI C'CCAG_C
Cuts at: 0 389 493 700 761 916 2213 2393 2685
Size: 389 104 207 61 155 1297 180 292
Fragments arranged by size:
1297 389 292 207 180 155 104 61
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 295 1129 1315 1372 2269 2685
Size: 295 834 186 57 897 416
Fragments arranged by size:
897 834 416 295 186 57
BsiEI CG_ry'CG
Cuts at: 0 1244 1653 2685
Size: 1244 409 1032
Fragments arranged by size:
1244 1032 409
BsiHKAI G_wGCw'C
Cuts at: 0 1142 1472 1574 1750 1808 2095 2149 2685
Size: 1142 330 102 176 58 287 54 536
Fragments arranged by size:
1142 536 330 287 176 102 58 54
BsmI GAATG_Cn'
Cuts at: 0 1482 1508 2685
Size: 1482 26 1177
Fragments arranged by size:
1482 1177 26
Bsp1286I G_dGCh'C
Cuts at: 0 18 44 142 264 292 462 469 485
Size: 18 26 98 122 28 170 7 16
Cuts at: 485 536 761 815 836 914 941 1073 1142
Size: 51 225 54 21 78 27 132 69
Cuts at: 1142 1203 1472 1574 1673 1713 1750 1808 1974
Size: 61 269 102 99 40 37 58 166
Cuts at: 1974 2027 2095 2109 2149 2376 2588 2685
Size: 53 68 14 40 227 212 97
Fragments arranged by size:
269 227 225 212 170 166 132 122
102 99 98 97 78 69 68 61
58 54 53 51 40 40 37 28
27 26 21 18 16 14 7
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 509 898 2685
Size: 509 389 1787
Fragments arranged by size:
1787 509 389
BsrBI CCG'CTC
Cuts at: 0 76 2132 2448 2685
Size: 76 2056 316 237
Fragments arranged by size:
2056 316 237 76
BsrDI GCAATG_nn'
Cuts at: 0 1127 1324 2685
Size: 1127 197 1361
Fragments arranged by size:
1361 1127 197
BsrFI r'CCGG_y
Cuts at: 0 81 983 2622 2685
Size: 81 902 1639 63
Fragments arranged by size:
1639 902 81 63
BssHII G'CGCG_C
Cuts at: 0 130 2685
Size: 130 2555
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1338 1512 2015 2066 2153 2175 2685
Size: 1338 174 503 51 87 22 510
Fragments arranged by size:
1338 510 503 174 87 51 22
BstXI CCAn_nnnn'nTGG
Cuts at: 0 591 988 2685
Size: 591 397 1697
Fragments arranged by size:
1697 591 397
BstYI r'GATC_y
Cuts at: 0 168 326 1956 2685
Size: 168 158 1630 729
Fragments arranged by size:
1630 729 168 158
Bsu36I CC'TnA_GG
Cuts at: 0 2378 2685
Size: 2378 307
BtgI C'CryG_G
Cuts at: 0 1254 1407 2685
Size: 1254 153 1278
Fragments arranged by size:
1278 1254 153
BtsI GCAGTG_nn'
Cuts at: 0 559 2055 2642 2685
Size: 559 1496 587 43
Fragments arranged by size:
1496 587 559 43
DraIII CAC_nnn'GTG
Cuts at: 0 2006 2175 2685
Size: 2006 169 510
Fragments arranged by size:
2006 510 169
EaeI y'GGCC_r
Cuts at: 0 6 53 357 581 971 1241 1689 2550
Size: 6 47 304 224 390 270 448 861
Cuts at: 2550 2685
Size: 135
Fragments arranged by size:
861 448 390 304 270 224 135 47
6
EagI C'GGCC_G
Cuts at: 0 1241 2685
Size: 1241 1444
EarI CTCTTCn'nnn_
Cuts at: 0 1040 2685
Size: 1040 1645
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 68 2685
Size: 68 2617
EcoICRI GAG'CTC
Cuts at: 0 1140 1572 1748 1806 2685
Size: 1140 432 176 58 879
Fragments arranged by size:
1140 879 432 176 58
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 125 197 304 472 550 1036 1363 1465
Size: 125 72 107 168 78 486 327 102
Cuts at: 1465 1537 1792 1888 1906 2254 2487 2685
Size: 72 255 96 18 348 233 198
Fragments arranged by size:
486 348 327 255 233 198 168 125
107 102 96 78 72 72 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1018 1186 2685
Size: 1018 168 1499
Fragments arranged by size:
1499 1018 168
EcoO109I rG'GnC_Cy
Cuts at: 0 261 385 412 588 709 812 911 938
Size: 261 124 27 176 121 103 99 27
Cuts at: 938 2584 2685
Size: 1646 101
Fragments arranged by size:
1646 261 176 124 121 103 101 99
27 27
HaeII r_GCGC'y
Cuts at: 0 29 74 209 1797 2220 2685
Size: 29 45 135 1588 423 465
Fragments arranged by size:
1588 465 423 135 45 29
HincII GTy'rAC
Cuts at: 0 1005 1103 2685
Size: 1005 98 1582
Fragments arranged by size:
1582 1005 98
KasI G'GCGC_C
Cuts at: 0 25 70 2685
Size: 25 45 2615
Fragments arranged by size:
2615 45 25
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 162 899 1775 2159 2685
Size: 162 737 876 384 526
Fragments arranged by size:
876 737 526 384 162
MscI TGG'CCA
Cuts at: 0 8 359 583 1691 2685
Size: 8 351 224 1108 994
Fragments arranged by size:
1108 994 351 224 8
MslI CAynn'nnrTG
Cuts at: 0 1475 2008 2685
Size: 1475 533 677
Fragments arranged by size:
1475 677 533
MspA1I CmG'CkG
Cuts at: 0 393 788 848 2495 2685
Size: 393 395 60 1647 190
Fragments arranged by size:
1647 395 393 190 60
NaeI GCC'GGC
Cuts at: 0 83 2685
Size: 83 2602
NarI GG'CG_CC
Cuts at: 0 26 71 2685
Size: 26 45 2614
Fragments arranged by size:
2614 45 26
NcoI C'CATG_G
Cuts at: 0 1407 2685
Size: 1407 1278
NgoMIV G'CCGG_C
Cuts at: 0 81 2685
Size: 81 2604
NsiI A_TGCA'T
Cuts at: 0 5 1508 2685
Size: 5 1503 1177
Fragments arranged by size:
1503 1177 5
NspI r_CATG'y
Cuts at: 0 1341 2013 2077 2467 2685
Size: 1341 672 64 390 218
Fragments arranged by size:
1341 672 390 218 64
PasI CC'CwG_GG
Cuts at: 0 599 934 2685
Size: 599 335 1751
Fragments arranged by size:
1751 599 335
PflMI CCAn_nnn'nTGG
Cuts at: 0 694 1182 1341 2685
Size: 694 488 159 1344
Fragments arranged by size:
1344 694 488 159
PfoI T'CCnGG_A
Cuts at: 0 1181 1985 2231 2685
Size: 1181 804 246 454
Fragments arranged by size:
1181 804 454 246
PpuMI rG'GwC_Cy
Cuts at: 0 385 412 709 2685
Size: 385 27 297 1976
Fragments arranged by size:
1976 385 297 27
PshAI GACnn'nnGTC
Cuts at: 0 410 1089 2685
Size: 410 679 1596
Fragments arranged by size:
1596 679 410
PspOMI G'GGCC_C
Cuts at: 0 260 465 811 910 937 2105 2584 2685
Size: 260 205 346 99 27 1168 479 101
Fragments arranged by size:
1168 479 346 260 205 101 99 27
PspXI vC'TCGA_Gb
Cuts at: 0 1801 2685
Size: 1801 884
PstI C_TGCA'G
Cuts at: 0 506 627 2200 2259 2278 2685
Size: 506 121 1573 59 19 407
Fragments arranged by size:
1573 506 407 121 59 19
PvuII CAG'CTG
Cuts at: 0 2495 2685
Size: 2495 190
SacI G_AGCT'C
Cuts at: 0 1142 1574 1750 1808 2685
Size: 1142 432 176 58 877
Fragments arranged by size:
1142 877 432 176 58
SfcI C'TryA_G
Cuts at: 0 502 623 2196 2255 2274 2524 2685
Size: 502 121 1573 59 19 250 161
Fragments arranged by size:
1573 502 250 161 121 59 19
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2558 2685
Size: 2558 127
SfoI GGC'GCC
Cuts at: 0 27 72 2685
Size: 27 45 2613
Fragments arranged by size:
2613 45 27
SgrAI Cr'CCGG_yG
Cuts at: 0 2622 2685
Size: 2622 63
SmaI CCC'GGG
Cuts at: 0 20 817 2582 2685
Size: 20 797 1765 103
Fragments arranged by size:
1765 797 103 20
SmlI C'TyrA_G
Cuts at: 0 1381 1594 1622 1801 2685
Size: 1381 213 28 179 884
Fragments arranged by size:
1381 884 213 179 28
SphI G_CATG'C
Cuts at: 0 2013 2685
Size: 2013 672
StuI AGG'CCT
Cuts at: 0 781 1159 2065 2245 2685
Size: 781 378 906 180 440
Fragments arranged by size:
906 781 440 378 180
StyI C'CwwG_G
Cuts at: 0 584 968 1407 1686 2351 2685
Size: 584 384 439 279 665 334
Fragments arranged by size:
665 584 439 384 334 279
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1643 1953 2494 2685
Size: 1643 310 541 191
Fragments arranged by size:
1643 541 310 191
Tth111I GACn'n_nGTC
Cuts at: 0 1249 1552 1840 2685
Size: 1249 303 288 845
Fragments arranged by size:
1249 845 303 288
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 1491 2685
Size: 1491 1194
XhoI C'TCGA_G
Cuts at: 0 1801 2685
Size: 1801 884
XmaI C'CCGG_G
Cuts at: 0 18 815 2580 2685
Size: 18 797 1765 105
Fragments arranged by size:
1765 797 105 18
Enzymes that do cut:
AcuI AfeI AflII AflIII AgeI AlfI AloI AlwNI
ApaI ApaLI AvaI BamHI BanI BanII BbeI BbsI
BbvCI BcgI BciVI BfrBI BglI Bme1580I BmrI BpmI
Bpu10I BpuEI BsaI BsaHI BsaWI BsaXI BseRI BseYI
BsgI BsiEI BsiHKAI BsmI Bsp1286I BspMI BsrBI BsrDI
BsrFI BssHII BstAPI BstXI BstYI Bsu36I BtgI BtsI
DraIII EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I HaeII HincII KasI MmeI MscI MslI MspA1I
NaeI NarI NcoI NgoMIV NsiI NspI PasI PflMI
PfoI PpuMI PshAI PspOMI PspXI PstI PvuII SacI
SfcI SfiI SfoI SgrAI SmaI SmlI SphI StuI
StyI TaqII Tth111I XcmI XhoI XmaI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AhdI AleI ApoI
AscI AseI AsiSI AvrII BaeI BclI BglII BlpI
BmgBI BmtI BplI BsaAI BsaBI BsiWI BsmBI BspEI
BspHI BsrGI BssSI BstBI BstEII BstZ17I BtgZI ClaI
CspCI DraI DrdI EcoRI EcoRV FalI FseI FspI
FspAI Hin4I HindIII HpaI KpnI MfeI MluI NdeI
NheI NotI NruI PacI PciI PmeI PmlI PpiI
PsiI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SgfI SnaBI SpeI SrfI SspI
SwaI TatI TstI XbaI XmnI ZraI