Restriction map for
pF1KB3684
(Linear) (Six-base) MAPSORT of: KIBB3684.seq Check: 472 from: 1 to: 1791
KIBB3684.orf
With 182 enzymes: SgfI *
March 2, 2010 14:25 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 895 1475 1791
Size: 895 580 316
Fragments arranged by size:
895 580 316
AccI GT'mk_AC
Cuts at: 0 1003 1791
Size: 1003 788
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 107 664 1547 1791
Size: 107 557 883 244
Fragments arranged by size:
883 557 244 107
AflIII A'CryG_T
Cuts at: 0 831 1791
Size: 831 960
AhdI GACnn_n'nnGTC
Cuts at: 0 417 1791
Size: 417 1374
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1138 1170 1791
Size: 1138 32 621
Fragments arranged by size:
1138 621 32
ApaI G_GGCC'C
Cuts at: 0 405 1266 1765 1791
Size: 405 861 499 26
Fragments arranged by size:
861 499 405 26
AseI AT'TA_AT
Cuts at: 0 266 1791
Size: 266 1525
BanI G'GyrC_C
Cuts at: 0 344 613 1515 1791
Size: 344 269 902 276
Fragments arranged by size:
902 344 276 269
BanII G_rGCy'C
Cuts at: 0 214 405 1205 1266 1457 1765 1791
Size: 214 191 800 61 191 308 26
Fragments arranged by size:
800 308 214 191 191 61 26
BbeI G_GCGC'C
Cuts at: 0 348 1791
Size: 348 1443
BbsI GAAGACnn'nnnn_
Cuts at: 0 1232 1791
Size: 1232 559
BfrBI ATG'CAT
Cuts at: 0 1647 1791
Size: 1647 144
BglI GCCn_nnn'nGGC
Cuts at: 0 523 1193 1791
Size: 523 670 598
Fragments arranged by size:
670 598 523
BglII A'GATC_T
Cuts at: 0 1508 1791
Size: 1508 283
Bme1580I G_kGCm'C
Cuts at: 0 405 501 771 1266 1373 1518 1765 1791
Size: 405 96 270 495 107 145 247 26
Fragments arranged by size:
495 405 270 247 145 107 96 26
BmrI ACTGGGnnnn_n'
Cuts at: 0 161 423 1260 1791
Size: 161 262 837 531
Fragments arranged by size:
837 531 262 161
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 123 1791
Size: 123 1668
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 1791
Size: 101 1690
Bpu10I CC'TnA_GC
Cuts at: 0 213 1791
Size: 213 1578
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 586 1102 1471 1791
Size: 586 516 369 320
Fragments arranged by size:
586 516 369 320
BsaBI GATnn'nnATC
Cuts at: 0 275 1791
Size: 275 1516
BsaHI Gr'CG_yC
Cuts at: 0 345 1316 1665 1791
Size: 345 971 349 126
Fragments arranged by size:
971 349 345 126
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 1424 1454 1791
Size: 1424 30 337
Fragments arranged by size:
1424 337 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 85 325 337 1236 1725 1768 1771 1791
Size: 85 240 12 899 489 43 3 20
Fragments arranged by size:
899 489 240 85 43 20 12 3
BseYI C'CCAG_C
Cuts at: 0 486 839 951 1729 1791
Size: 486 353 112 778 62
Fragments arranged by size:
778 486 353 112 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 559 580 1791
Size: 559 21 1211
Fragments arranged by size:
1211 559 21
BsiHKAI G_wGCw'C
Cuts at: 0 214 1205 1791
Size: 214 991 586
Fragments arranged by size:
991 586 214
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1656 1791
Size: 1656 135
Bsp1286I G_dGCh'C
Cuts at: 0 214 405 501 771 1205 1266 1373 1457
Size: 214 191 96 270 434 61 107 84
Cuts at: 1457 1518 1765 1791
Size: 61 247 26
Fragments arranged by size:
434 270 247 214 191 107 96 84
61 61 26
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 771 895 1475 1791
Size: 771 124 580 316
Fragments arranged by size:
771 580 316 124
BsrBI CCG'CTC
Cuts at: 0 293 399 480 1355 1584 1791
Size: 293 106 81 875 229 207
Fragments arranged by size:
875 293 229 207 106 81
BsrFI r'CCGG_y
Cuts at: 0 1673 1791
Size: 1673 118
BsrGI T'GTAC_A
Cuts at: 0 351 1181 1791
Size: 351 830 610
Fragments arranged by size:
830 610 351
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1782 1791
Size: 1782 9
BstYI r'GATC_y
Cuts at: 0 1508 1791
Size: 1508 283
BstZ17I GTA'TAC
Cuts at: 0 1004 1791
Size: 1004 787
Bsu36I CC'TnA_GG
Cuts at: 0 108 1791
Size: 108 1683
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 650 1791
Size: 650 1141
BtsI GCAGTG_nn'
Cuts at: 0 1049 1783 1791
Size: 1049 734 8
Fragments arranged by size:
1049 734 8
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 815 850 1791
Size: 815 35 941
Fragments arranged by size:
941 815 35
DraIII CAC_nnn'GTG
Cuts at: 0 812 1143 1791
Size: 812 331 648
Fragments arranged by size:
812 648 331
EaeI y'GGCC_r
Cuts at: 0 623 699 758 1208 1791
Size: 623 76 59 450 583
Fragments arranged by size:
623 583 450 76 59
EarI CTCTTCn'nnn_
Cuts at: 0 83 296 302 314 326 1243 1791
Size: 83 213 6 12 12 917 548
Fragments arranged by size:
917 548 213 83 12 12 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 538 1791
Size: 538 1253
EcoICRI GAG'CTC
Cuts at: 0 212 1203 1791
Size: 212 991 588
Fragments arranged by size:
991 588 212
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 101 107 664 1547 1791
Size: 101 6 557 883 244
Fragments arranged by size:
883 557 244 101 6
EcoO109I rG'GnC_Cy
Cuts at: 0 402 1262 1761 1762 1791
Size: 402 860 499 1 29
Fragments arranged by size:
860 499 402 29 1
HaeII r_GCGC'y
Cuts at: 0 348 1680 1791
Size: 348 1332 111
Fragments arranged by size:
1332 348 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 61 93 214 246 367 399 403 435
Size: 61 32 121 32 121 32 4 32
Cuts at: 435 1378 1410 1653 1685 1699 1731 1791
Size: 943 32 243 32 14 32 60
Fragments arranged by size:
943 243 121 121 61 60 32 32
32 32 32 32 32 14 4
HindIII A'AGCT_T
Cuts at: 0 1529 1791
Size: 1529 262
KasI G'GCGC_C
Cuts at: 0 344 1791
Size: 344 1447
MscI TGG'CCA
Cuts at: 0 625 701 760 1210 1791
Size: 625 76 59 450 581
Fragments arranged by size:
625 581 450 76 59
MslI CAynn'nnrTG
Cuts at: 0 118 1256 1791
Size: 118 1138 535
Fragments arranged by size:
1138 535 118
MspA1I CmG'CkG
Cuts at: 0 1525 1791
Size: 1525 266
NarI GG'CG_CC
Cuts at: 0 345 1791
Size: 345 1446
NsiI A_TGCA'T
Cuts at: 0 1649 1791
Size: 1649 142
NspI r_CATG'y
Cuts at: 0 1632 1785 1791
Size: 1632 153 6
Fragments arranged by size:
1632 153 6
PflMI CCAn_nnn'nTGG
Cuts at: 0 864 1791
Size: 864 927
PshAI GACnn'nnGTC
Cuts at: 0 251 1791
Size: 251 1540
PspOMI G'GGCC_C
Cuts at: 0 401 1262 1761 1791
Size: 401 861 499 30
Fragments arranged by size:
861 499 401 30
PstI C_TGCA'G
Cuts at: 0 557 906 1791
Size: 557 349 885
Fragments arranged by size:
885 557 349
PvuII CAG'CTG
Cuts at: 0 1525 1791
Size: 1525 266
SacI G_AGCT'C
Cuts at: 0 214 1205 1791
Size: 214 991 586
Fragments arranged by size:
991 586 214
SapI GCTCTTCn'nnn_
Cuts at: 0 326 1791
Size: 326 1465
ScaI AGT'ACT
Cuts at: 0 683 1115 1791
Size: 683 432 676
Fragments arranged by size:
683 676 432
SexAI A'CCwGG_T
Cuts at: 0 1139 1791
Size: 1139 652
SfcI C'TryA_G
Cuts at: 0 553 902 1791
Size: 553 349 889
Fragments arranged by size:
889 553 349
SfoI GGC'GCC
Cuts at: 0 346 1791
Size: 346 1445
SgrAI Cr'CCGG_yG
Cuts at: 0 1673 1791
Size: 1673 118
SmlI C'TyrA_G
Cuts at: 0 565 1117 1450 1791
Size: 565 552 333 341
Fragments arranged by size:
565 552 341 333
SphI G_CATG'C
Cuts at: 0 1785 1791
Size: 1785 6
StuI AGG'CCT
Cuts at: 0 127 517 1791
Size: 127 390 1274
Fragments arranged by size:
1274 390 127
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 405 1036 1533 1791
Size: 405 631 497 258
Fragments arranged by size:
631 497 405 258
TatI w'GTAC_w
Cuts at: 0 351 681 1113 1181 1791
Size: 351 330 432 68 610
Fragments arranged by size:
610 432 351 330 68
Enzymes that do cut:
AarI AccI AcuI AflIII AhdI AloI ApaI AseI
BanI BanII BbeI BbsI BfrBI BglI BglII Bme1580I
BmrI BplI BpmI Bpu10I BpuEI BsaBI BsaHI BsaXI
BseRI BseYI BsgI BsiHKAI BsmBI Bsp1286I BspMI BsrBI
BsrFI BsrGI BstAPI BstYI BstZ17I Bsu36I BtgZI BtsI
CspCI DraIII EaeI EarI EciI EcoICRI Eco57MI EcoO109I
HaeII Hin4I HindIII KasI MscI MslI MspA1I NarI
NsiI NspI PflMI PshAI PspOMI PstI PvuII SacI
SapI ScaI SexAI SfcI SfoI SgrAI SmlI SphI
StuI TaqII TatI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AleI AlfI
AlwNI ApaLI ApoI AscI AsiSI AvaI AvrII BaeI
BamHI BbvCI BcgI BciVI BclI BlpI BmgBI BmtI
BsaI BsaAI BsaWI BsiEI BsiWI BsmI BspEI BspHI
BsrDI BssHII BssSI BstBI BstEII BstXI BtgI ClaI
DraI DrdI EagI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HincII HpaI KpnI MfeI MluI MmeI
NaeI NcoI NdeI NgoMIV NheI NotI NruI PacI
PasI PciI PfoI PmeI PmlI PpiI PpuMI PsiI
PspXI PsrI PvuI RsrII SacII SalI SanDI SbfI
SfiI SgfI SmaI SnaBI SpeI SrfI SspI StyI
SwaI TstI Tth111I XbaI XcmI XhoI XmaI XmnI
ZraI