(Linear) (Six-base) MAPSORT of: KIBB3692.seq Check: 335 from: 1 to: 384
KIBB3692.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AatII G_ACGT'C
Cuts at: 0 224 384
Size: 224 160
BbsI GAAGACnn'nnnn_
Cuts at: 0 45 384
Size: 45 339
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 255 384
Size: 255 129
BsaHI Gr'CG_yC
Cuts at: 0 221 384
Size: 221 163
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 5 35 384
Size: 5 30 349
Fragments arranged by size:
349 30 5
BseYI C'CCAG_C
Cuts at: 0 166 384
Size: 166 218
BsrDI GCAATG_nn'
Cuts at: 0 204 384
Size: 204 180
BtsI GCAGTG_nn'
Cuts at: 0 85 384
Size: 85 299
EcoO109I rG'GnC_Cy
Cuts at: 0 273 384
Size: 273 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 5 37 363 384
Size: 5 32 326 21
Fragments arranged by size:
326 32 21 5
MfeI C'AATT_G
Cuts at: 0 126 384
Size: 126 258
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 172 384
Size: 172 212
PpuMI rG'GwC_Cy
Cuts at: 0 273 384
Size: 273 111
PstI C_TGCA'G
Cuts at: 0 80 384
Size: 80 304
SfcI C'TryA_G
Cuts at: 0 76 384
Size: 76 308
SmlI C'TyrA_G
Cuts at: 0 234 384
Size: 234 150
StyI C'CwwG_G
Cuts at: 0 9 384
Size: 9 375
ZraI GAC'GTC
Cuts at: 0 222 384
Size: 222 162
Enzymes that do cut:
AatII BbsI BpuEI BsaHI BsaXI BseYI BsrDI BtsI
EcoO109I Hin4I MfeI MmeI PpuMI PstI SfcI SmlI
StyI ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI
ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI
BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI
BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MluI
MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI PacI PasI PciI
PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI