(Linear) (Six-base) MAPSORT of: KIBB3692.seq Check: 335 from: 1 to: 384 KIBB3692.orf With 182 enzymes: SgfI * April 24, 2009 13:02 .. AatII G_ACGT'C Cuts at: 0 224 384 Size: 224 160 BbsI GAAGACnn'nnnn_ Cuts at: 0 45 384 Size: 45 339 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 0 255 384 Size: 255 129 BsaHI Gr'CG_yC Cuts at: 0 221 384 Size: 221 163 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 0 5 35 384 Size: 5 30 349 Fragments arranged by size: 349 30 5 BseYI C'CCAG_C Cuts at: 0 166 384 Size: 166 218 BsrDI GCAATG_nn' Cuts at: 0 204 384 Size: 204 180 BtsI GCAGTG_nn' Cuts at: 0 85 384 Size: 85 299 EcoO109I rG'GnC_Cy Cuts at: 0 273 384 Size: 273 111 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 0 5 37 363 384 Size: 5 32 326 21 Fragments arranged by size: 326 32 21 5 MfeI C'AATT_G Cuts at: 0 126 384 Size: 126 258 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 0 172 384 Size: 172 212 PpuMI rG'GwC_Cy Cuts at: 0 273 384 Size: 273 111 PstI C_TGCA'G Cuts at: 0 80 384 Size: 80 304 SfcI C'TryA_G Cuts at: 0 76 384 Size: 76 308 SmlI C'TyrA_G Cuts at: 0 234 384 Size: 234 150 StyI C'CwwG_G Cuts at: 0 9 384 Size: 9 375 ZraI GAC'GTC Cuts at: 0 222 384 Size: 222 162 Enzymes that do cut: AatII BbsI BpuEI BsaHI BsaXI BseYI BsrDI BtsI EcoO109I Hin4I MfeI MmeI PpuMI PstI SfcI SmlI StyI ZraI Enzymes that do not cut: AarI AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BsaI BsaAI BsaBI BsaWI BseRI BsgI BsiEI BsiHKAI BsiWI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoRI EcoRV FalI FseI FspI FspAI HaeII HincII HindIII HpaI KasI KpnI MluI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspI PacI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI PspXI PsrI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI SwaI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI