Restriction map for
pF1KB3698
(Linear) (Six-base) MAPSORT of: KIBB3698.seq Check: 2747 from: 1 to: 1125
KIBB3698.orf
With 182 enzymes: SgfI *
July 18, 2007 13:36 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 599 1125
Size: 599 526
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 975 1087 1125
Size: 975 112 38
Fragments arranged by size:
975 112 38
AflIII A'CryG_T
Cuts at: 0 419 1125
Size: 419 706
AleI CACnn'nnGTG
Cuts at: 0 568 583 1125
Size: 568 15 542
Fragments arranged by size:
568 542 15
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1093 1125
Size: 1093 32
ApaLI G'TGCA_C
Cuts at: 0 387 586 841 1125
Size: 387 199 255 284
Fragments arranged by size:
387 284 255 199
ApoI r'AATT_y
Cuts at: 0 1029 1125
Size: 1029 96
AvaI C'yCGr_G
Cuts at: 0 20 85 649 979 1125
Size: 20 65 564 330 146
Fragments arranged by size:
564 330 146 65 20
BanI G'GyrC_C
Cuts at: 0 49 115 570 737 1125
Size: 49 66 455 167 388
Fragments arranged by size:
455 388 167 66 49
BanII G_rGCy'C
Cuts at: 0 92 146 1125
Size: 92 54 979
Fragments arranged by size:
979 92 54
BbsI GAAGACnn'nnnn_
Cuts at: 0 426 792 822 1125
Size: 426 366 30 303
Fragments arranged by size:
426 366 303 30
BbvCI CC'TCA_GC
Cuts at: 0 1003 1125
Size: 1003 122
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 765 799 1125
Size: 765 34 326
Fragments arranged by size:
765 326 34
BglI GCCn_nnn'nGGC
Cuts at: 0 759 1069 1125
Size: 759 310 56
Fragments arranged by size:
759 310 56
BlpI GC'TnA_GC
Cuts at: 0 526 1125
Size: 526 599
Bme1580I G_kGCm'C
Cuts at: 0 118 391 575 590 677 845 1125
Size: 118 273 184 15 87 168 280
Fragments arranged by size:
280 273 184 168 118 87 15
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 1125
Size: 55 1070
Bpu10I CC'TnA_GC
Cuts at: 0 1003 1125
Size: 1003 122
BsaI GGTCTCn'nnnn_
Cuts at: 0 979 1125
Size: 979 146
BsaAI yAC'GTr
Cuts at: 0 165 1125
Size: 165 960
BsaBI GATnn'nnATC
Cuts at: 0 274 1125
Size: 274 851
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 64 94 628 658 1125
Size: 64 30 534 30 467
Fragments arranged by size:
534 467 64 30 30
BseYI C'CCAG_C
Cuts at: 0 660 1125
Size: 660 465
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 340 661 1125
Size: 340 321 464
Fragments arranged by size:
464 340 321
BsiEI CG_ry'CG
Cuts at: 0 462 1125
Size: 462 663
BsiHKAI G_wGCw'C
Cuts at: 0 92 146 155 391 590 845 1125
Size: 92 54 9 236 199 255 280
Fragments arranged by size:
280 255 236 199 92 54 9
Bsp1286I G_dGCh'C
Cuts at: 0 92 118 146 155 391 575 590 677
Size: 92 26 28 9 236 184 15 87
Cuts at: 677 845 1125
Size: 168 280
Fragments arranged by size:
280 236 184 168 92 87 28 26
15 9
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 362 402 599 1125
Size: 362 40 197 526
Fragments arranged by size:
526 362 197 40
BsrBI CCG'CTC
Cuts at: 0 108 363 874 1125
Size: 108 255 511 251
Fragments arranged by size:
511 255 251 108
BsrFI r'CCGG_y
Cuts at: 0 734 1035 1125
Size: 734 301 90
Fragments arranged by size:
734 301 90
BsrGI T'GTAC_A
Cuts at: 0 422 1010 1125
Size: 422 588 115
Fragments arranged by size:
588 422 115
BssSI C'ACGA_G
Cuts at: 0 183 358 1125
Size: 183 175 767
Fragments arranged by size:
767 183 175
BstAPI GCAn_nnn'nTGC
Cuts at: 0 849 1125
Size: 849 276
BtgI C'CryG_G
Cuts at: 0 741 1125
Size: 741 384
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 360 453 1125
Size: 360 93 672
Fragments arranged by size:
672 360 93
BtsI GCAGTG_nn'
Cuts at: 0 649 1122 1125
Size: 649 473 3
Fragments arranged by size:
649 473 3
DrdI GACnn_nn'nnGTC
Cuts at: 0 379 1125
Size: 379 746
EaeI y'GGCC_r
Cuts at: 0 125 326 476 1125
Size: 125 201 150 649
Fragments arranged by size:
649 201 150 125
EarI CTCTTCn'nnn_
Cuts at: 0 211 409 1125
Size: 211 198 716
Fragments arranged by size:
716 211 198
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 325 883 1125
Size: 325 558 242
Fragments arranged by size:
558 325 242
EcoICRI GAG'CTC
Cuts at: 0 90 144 1125
Size: 90 54 981
Fragments arranged by size:
981 90 54
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 55 975 1087 1125
Size: 55 920 112 38
Fragments arranged by size:
920 112 55 38
EcoNI CCTnn'n_nnAGG
Cuts at: 0 340 1125
Size: 340 785
HaeII r_GCGC'y
Cuts at: 0 772 1125
Size: 772 353
HincII GTy'rAC
Cuts at: 0 417 927 1125
Size: 417 510 198
Fragments arranged by size:
510 417 198
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 968 1079 1125
Size: 968 111 46
Fragments arranged by size:
968 111 46
MscI TGG'CCA
Cuts at: 0 127 478 1125
Size: 127 351 647
Fragments arranged by size:
647 351 127
MslI CAynn'nnrTG
Cuts at: 0 322 343 394 448 544 568 583 838
Size: 322 21 51 54 96 24 15 255
Cuts at: 838 1125
Size: 287
Fragments arranged by size:
322 287 255 96 54 51 24 21
15
MspA1I CmG'CkG
Cuts at: 0 855 950 1125
Size: 855 95 175
Fragments arranged by size:
855 175 95
NaeI GCC'GGC
Cuts at: 0 1037 1125
Size: 1037 88
NgoMIV G'CCGG_C
Cuts at: 0 1035 1125
Size: 1035 90
NspI r_CATG'y
Cuts at: 0 423 1125
Size: 423 702
PciI A'CATG_T
Cuts at: 0 419 1125
Size: 419 706
PfoI T'CCnGG_A
Cuts at: 0 632 1125
Size: 632 493
PspXI vC'TCGA_Gb
Cuts at: 0 649 1125
Size: 649 476
PstI C_TGCA'G
Cuts at: 0 413 496 596 854 1125
Size: 413 83 100 258 271
Fragments arranged by size:
413 271 258 100 83
PvuII CAG'CTG
Cuts at: 0 950 1125
Size: 950 175
SacI G_AGCT'C
Cuts at: 0 92 146 1125
Size: 92 54 979
Fragments arranged by size:
979 92 54
SapI GCTCTTCn'nnn_
Cuts at: 0 409 1125
Size: 409 716
SbfI CC_TGCA'GG
Cuts at: 0 413 1125
Size: 413 712
SfcI C'TryA_G
Cuts at: 0 409 492 592 850 969 1125
Size: 409 83 100 258 119 156
Fragments arranged by size:
409 258 156 119 100 83
SmaI CCC'GGG
Cuts at: 0 22 1125
Size: 22 1103
SmlI C'TyrA_G
Cuts at: 0 649 1125
Size: 649 476
StuI AGG'CCT
Cuts at: 0 610 1125
Size: 610 515
TatI w'GTAC_w
Cuts at: 0 287 422 1010 1125
Size: 287 135 588 115
Fragments arranged by size:
588 287 135 115
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 628 660 1125
Size: 628 32 465
Fragments arranged by size:
628 465 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 219 1125
Size: 219 906
XhoI C'TCGA_G
Cuts at: 0 649 1125
Size: 649 476
XmaI C'CCGG_G
Cuts at: 0 20 1125
Size: 20 1105
XmnI GAAnn'nnTTC
Cuts at: 0 829 1048 1125
Size: 829 219 77
Fragments arranged by size:
829 219 77
Enzymes that do cut:
AarI AcuI AflIII AleI AloI ApaLI ApoI AvaI
BanI BanII BbsI BbvCI BcgI BglI BlpI Bme1580I
BpmI Bpu10I BsaI BsaAI BsaBI BsaXI BseYI BsgI
BsiEI BsiHKAI Bsp1286I BspMI BsrBI BsrFI BsrGI BssSI
BstAPI BtgI BtgZI BtsI DrdI EaeI EarI EciI
EcoICRI Eco57MI EcoNI HaeII HincII MmeI MscI MslI
MspA1I NaeI NgoMIV NspI PciI PfoI PspXI PstI
PvuII SacI SapI SbfI SfcI SmaI SmlI StuI
TatI TstI XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AfeI AflII AgeI AhdI
AlfI AlwNI ApaI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BciVI BclI BfrBI BglII BmgBI BmrI
BmtI BplI BpuEI BsaHI BsaWI BseRI BsiWI BsmI
BsmBI BspEI BspHI BsrDI BssHII BstBI BstEII BstXI
BstYI BstZ17I Bsu36I ClaI CspCI DraI DraIII EagI
EcoO109I EcoRI EcoRV FalI FseI FspI FspAI Hin4I
HindIII HpaI KasI KpnI MfeI MluI NarI NcoI
NdeI NheI NotI NruI NsiI PacI PasI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PsrI
PvuI RsrII SacII SalI SanDI ScaI SexAI SfiI
SfoI SgfI SgrAI SnaBI SpeI SphI SrfI SspI
StyI SwaI TaqII Tth111I XbaI ZraI