Restriction map for
pF1KB3700
(Linear) (Six-base) MAPSORT of: KIBB3700.seq Check: 2392 from: 1 to: 1920
KIBB3700.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1525 1920
Size: 1525 395
AccI GT'mk_AC
Cuts at: 0 1234 1920
Size: 1234 686
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1780 1920
Size: 1780 140
AflIII A'CryG_T
Cuts at: 0 986 1563 1920
Size: 986 577 357
Fragments arranged by size:
986 577 357
AleI CACnn'nnGTG
Cuts at: 0 772 1468 1920
Size: 772 696 452
Fragments arranged by size:
772 696 452
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 88 1920
Size: 88 1832
AlwNI CAG_nnn'CTG
Cuts at: 0 528 712 1818 1876 1920
Size: 528 184 1106 58 44
Fragments arranged by size:
1106 528 184 58 44
ApaI G_GGCC'C
Cuts at: 0 140 881 1920
Size: 140 741 1039
Fragments arranged by size:
1039 741 140
ApaLI G'TGCA_C
Cuts at: 0 661 1783 1885 1920
Size: 661 1122 102 35
Fragments arranged by size:
1122 661 102 35
ApoI r'AATT_y
Cuts at: 0 1768 1920
Size: 1768 152
AvaI C'yCGr_G
Cuts at: 0 275 1390 1920
Size: 275 1115 530
Fragments arranged by size:
1115 530 275
BanI G'GyrC_C
Cuts at: 0 1081 1666 1920
Size: 1081 585 254
Fragments arranged by size:
1081 585 254
BanII G_rGCy'C
Cuts at: 0 140 587 881 1508 1528 1920
Size: 140 447 294 627 20 392
Fragments arranged by size:
627 447 392 294 140 20
BbeI G_GCGC'C
Cuts at: 0 1085 1920
Size: 1085 835
BbsI GAAGACnn'nnnn_
Cuts at: 0 1362 1452 1920
Size: 1362 90 468
Fragments arranged by size:
1362 468 90
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 242 276 1920
Size: 242 34 1644
Fragments arranged by size:
1644 242 34
BclI T'GATC_A
Cuts at: 0 1025 1619 1920
Size: 1025 594 301
Fragments arranged by size:
1025 594 301
BglI GCCn_nnn'nGGC
Cuts at: 0 233 508 674 730 1247 1439 1920
Size: 233 275 166 56 517 192 481
Fragments arranged by size:
517 481 275 233 192 166 56
BlpI GC'TnA_GC
Cuts at: 0 1717 1920
Size: 1717 203
Bme1580I G_kGCm'C
Cuts at: 0 130 140 665 881 1475 1669 1787 1889
Size: 130 10 525 216 594 194 118 102
Cuts at: 1889 1920
Size: 31
Fragments arranged by size:
594 525 216 194 130 118 102 31
10
BmgBI CAC'GTC
Cuts at: 0 399 987 1058 1920
Size: 399 588 71 862
Fragments arranged by size:
862 588 399 71
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 1542 1795 1920
Size: 903 639 253 125
Fragments arranged by size:
903 639 253 125
Bpu10I CC'TnA_GC
Cuts at: 0 1042 1920
Size: 1042 878
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1570 1920
Size: 1570 350
BsaBI GATnn'nnATC
Cuts at: 0 1210 1920
Size: 1210 710
BsaHI Gr'CG_yC
Cuts at: 0 1082 1920
Size: 1082 838
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 271 301 436 442 466 472 1628 1658
Size: 271 30 135 6 24 6 1156 30
Cuts at: 1658 1920
Size: 262
Fragments arranged by size:
1156 271 262 135 30 30 24 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 913 1078 1309 1920
Size: 913 165 231 611
Fragments arranged by size:
913 611 231 165
BseYI C'CCAG_C
Cuts at: 0 1544 1858 1920
Size: 1544 314 62
Fragments arranged by size:
1544 314 62
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 970 1678 1920
Size: 202 768 708 242
Fragments arranged by size:
768 708 242 202
BsiHKAI G_wGCw'C
Cuts at: 0 77 665 1528 1787 1889 1920
Size: 77 588 863 259 102 31
Fragments arranged by size:
863 588 259 102 77 31
Bsp1286I G_dGCh'C
Cuts at: 0 77 130 140 587 665 881 1475 1508
Size: 77 53 10 447 78 216 594 33
Cuts at: 1508 1528 1669 1787 1889 1920
Size: 20 141 118 102 31
Fragments arranged by size:
594 447 216 141 118 102 78 77
53 33 31 20 10
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 224 1525 1874 1920
Size: 224 1301 349 46
Fragments arranged by size:
1301 349 224 46
BsrBI CCG'CTC
Cuts at: 0 864 927 1649 1920
Size: 864 63 722 271
Fragments arranged by size:
864 722 271 63
BsrDI GCAATG_nn'
Cuts at: 0 471 1920
Size: 471 1449
BsrFI r'CCGG_y
Cuts at: 0 59 413 992 1339 1850 1920
Size: 59 354 579 347 511 70
Fragments arranged by size:
579 511 354 347 70 59
BsrGI T'GTAC_A
Cuts at: 0 1734 1920
Size: 1734 186
BssSI C'ACGA_G
Cuts at: 0 6 1920
Size: 6 1914
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1323 1920
Size: 1323 597
BstBI TT'CG_AA
Cuts at: 0 1175 1920
Size: 1175 745
BstEII G'GTnAC_C
Cuts at: 0 1551 1920
Size: 1551 369
Bsu36I CC'TnA_GG
Cuts at: 0 46 1920
Size: 46 1874
BtgI C'CryG_G
Cuts at: 0 795 1116 1920
Size: 795 321 804
Fragments arranged by size:
804 795 321
BtsI GCAGTG_nn'
Cuts at: 0 707 1920
Size: 707 1213
ClaI AT'CG_AT
Cuts at: 0 407 1920
Size: 407 1513
DraIII CAC_nnn'GTG
Cuts at: 0 1755 1920
Size: 1755 165
EaeI y'GGCC_r
Cuts at: 0 1222 1265 1880 1920
Size: 1222 43 615 40
Fragments arranged by size:
1222 615 43 40
EarI CTCTTCn'nnn_
Cuts at: 0 82 97 1920
Size: 82 15 1823
Fragments arranged by size:
1823 82 15
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 829 1920
Size: 829 1091
EcoICRI GAG'CTC
Cuts at: 0 1526 1920
Size: 1526 394
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 903 1542 1780 1795 1920
Size: 903 639 238 15 125
Fragments arranged by size:
903 639 238 125 15
EcoNI CCTnn'n_nnAGG
Cuts at: 0 220 598 745 1920
Size: 220 378 147 1175
Fragments arranged by size:
1175 378 220 147
EcoO109I rG'GnC_Cy
Cuts at: 0 84 136 177 327 525 878 1101 1815
Size: 84 52 41 150 198 353 223 714
Cuts at: 1815 1920
Size: 105
Fragments arranged by size:
714 353 223 198 150 105 84 52
41
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 93 1164 1920
Size: 93 1071 756
Fragments arranged by size:
1071 756 93
FspI TGC'GCA
Cuts at: 0 778 847 1378 1920
Size: 778 69 531 542
Fragments arranged by size:
778 542 531 69
HaeII r_GCGC'y
Cuts at: 0 381 1085 1920
Size: 381 704 835
Fragments arranged by size:
835 704 381
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1459 1491 1628 1660 1920
Size: 1459 32 137 32 260
Fragments arranged by size:
1459 260 137 32 32
HincII GTy'rAC
Cuts at: 0 429 1920
Size: 429 1491
KasI G'GCGC_C
Cuts at: 0 1081 1920
Size: 1081 839
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 479 809 1502 1920
Size: 479 330 693 418
Fragments arranged by size:
693 479 418 330
MscI TGG'CCA
Cuts at: 0 1267 1920
Size: 1267 653
MslI CAynn'nnrTG
Cuts at: 0 772 1468 1920
Size: 772 696 452
Fragments arranged by size:
772 696 452
MspA1I CmG'CkG
Cuts at: 0 712 1118 1221 1548 1920
Size: 712 406 103 327 372
Fragments arranged by size:
712 406 372 327 103
NaeI GCC'GGC
Cuts at: 0 61 1341 1852 1920
Size: 61 1280 511 68
Fragments arranged by size:
1280 511 68 61
NarI GG'CG_CC
Cuts at: 0 1082 1920
Size: 1082 838
NgoMIV G'CCGG_C
Cuts at: 0 59 1339 1850 1920
Size: 59 1280 511 70
Fragments arranged by size:
1280 511 70 59
NspI r_CATG'y
Cuts at: 0 654 1317 1567 1920
Size: 654 663 250 353
Fragments arranged by size:
663 654 353 250
PasI CC'CwG_GG
Cuts at: 0 854 1920
Size: 854 1066
PciI A'CATG_T
Cuts at: 0 1563 1920
Size: 1563 357
PflMI CCAn_nnn'nTGG
Cuts at: 0 528 1920
Size: 528 1392
PfoI T'CCnGG_A
Cuts at: 0 1835 1920
Size: 1835 85
PpuMI rG'GwC_Cy
Cuts at: 0 84 177 327 1101 1920
Size: 84 93 150 774 819
Fragments arranged by size:
819 774 150 93 84
PspOMI G'GGCC_C
Cuts at: 0 136 877 1920
Size: 136 741 1043
Fragments arranged by size:
1043 741 136
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1150 1182 1291 1323 1920
Size: 1150 32 109 32 597
Fragments arranged by size:
1150 597 109 32 32
PstI C_TGCA'G
Cuts at: 0 1328 1920
Size: 1328 592
PvuII CAG'CTG
Cuts at: 0 712 1548 1920
Size: 712 836 372
Fragments arranged by size:
836 712 372
SacI G_AGCT'C
Cuts at: 0 1528 1920
Size: 1528 392
SacII CC_GC'GG
Cuts at: 0 1119 1920
Size: 1119 801
SanDI GG'GwC_CC
Cuts at: 0 327 1920
Size: 327 1593
SapI GCTCTTCn'nnn_
Cuts at: 0 82 1920
Size: 82 1838
SfcI C'TryA_G
Cuts at: 0 1324 1920
Size: 1324 596
SfoI GGC'GCC
Cuts at: 0 1083 1920
Size: 1083 837
SmaI CCC'GGG
Cuts at: 0 277 1920
Size: 277 1643
SmlI C'TyrA_G
Cuts at: 0 1585 1920
Size: 1585 335
SphI G_CATG'C
Cuts at: 0 1317 1920
Size: 1317 603
StuI AGG'CCT
Cuts at: 0 496 1920
Size: 496 1424
StyI C'CwwG_G
Cuts at: 0 161 1920
Size: 161 1759
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 381 613 928 1856 1920
Size: 381 232 315 928 64
Fragments arranged by size:
928 381 315 232 64
TatI w'GTAC_w
Cuts at: 0 1166 1682 1734 1920
Size: 1166 516 52 186
Fragments arranged by size:
1166 516 186 52
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 271 303 442 474 1920
Size: 271 32 139 32 1446
Fragments arranged by size:
1446 271 139 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 400 1642 1920
Size: 400 1242 278
Fragments arranged by size:
1242 400 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 529 1517 1920
Size: 529 988 403
Fragments arranged by size:
988 529 403
XmaI C'CCGG_G
Cuts at: 0 275 1920
Size: 275 1645
Enzymes that do cut:
AarI AccI AcuI AflIII AleI AlfI AlwNI ApaI
ApaLI ApoI AvaI BanI BanII BbeI BbsI BcgI
BclI BglI BlpI Bme1580I BmgBI BpmI Bpu10I BpuEI
BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiHKAI Bsp1286I
BspMI BsrBI BsrDI BsrFI BsrGI BssSI BstAPI BstBI
BstEII Bsu36I BtgI BtsI ClaI DraIII EaeI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FspI HaeII
Hin4I HincII KasI MmeI MscI MslI MspA1I NaeI
NarI NgoMIV NspI PasI PciI PflMI PfoI PpuMI
PspOMI PsrI PstI PvuII SacI SacII SanDI SapI
SfcI SfoI SmaI SmlI SphI StuI StyI TaqII
TatI TstI Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AgeI AhdI AloI
AscI AseI AsiSI AvrII BaeI BamHI BbvCI BciVI
BfrBI BglII BmrI BmtI BplI BsaI BsaAI BsaWI
BsiEI BsiWI BsmI BsmBI BspEI BspHI BssHII BstXI
BstYI BstZ17I BtgZI CspCI DraI DrdI EagI EcoRI
EcoRV FseI FspAI HindIII HpaI KpnI MfeI MluI
NcoI NdeI NheI NotI NruI NsiI PacI PmeI
PmlI PpiI PshAI PsiI PspXI PvuI RsrII SalI
SbfI ScaI SexAI SfiI SgfI SgrAI SnaBI SpeI
SrfI SspI SwaI XbaI XhoI XmnI ZraI