Restriction map for
pF1KB3701
(Linear) (Six-base) MAPSORT of: KIBB3701.seq Check: 7805 from: 1 to: 3534
KIBB3701.orf
With 182 enzymes: SgfI *
April 24, 2009 13:02 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 978 3534
Size: 978 2556
AatII G_ACGT'C
Cuts at: 0 1841 3534
Size: 1841 1693
AccI GT'mk_AC
Cuts at: 0 1025 2984 3534
Size: 1025 1959 550
Fragments arranged by size:
1959 1025 550
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 1692 1776 1788 2260 2923 3534
Size: 25 1667 84 12 472 663 611
Fragments arranged by size:
1667 663 611 472 84 25 12
AflIII A'CryG_T
Cuts at: 0 2321 2579 3534
Size: 2321 258 955
Fragments arranged by size:
2321 955 258
AleI CACnn'nnGTG
Cuts at: 0 1741 2473 3534
Size: 1741 732 1061
Fragments arranged by size:
1741 1061 732
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3187 3534
Size: 3187 347
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1420 1452 3534
Size: 1420 32 2082
Fragments arranged by size:
2082 1420 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1413 1423 1728 1923 2700 2967 3408 3534
Size: 1413 10 305 195 777 267 441 126
Fragments arranged by size:
1413 777 441 305 267 195 126 10
ApaI G_GGCC'C
Cuts at: 0 1338 1491 2436 3534
Size: 1338 153 945 1098
Fragments arranged by size:
1338 1098 945 153
ApaLI G'TGCA_C
Cuts at: 0 340 619 3534
Size: 340 279 2915
Fragments arranged by size:
2915 340 279
AvaI C'yCGr_G
Cuts at: 0 464 653 1661 1680 2522 3534
Size: 464 189 1008 19 842 1012
Fragments arranged by size:
1012 1008 842 464 189 19
BanI G'GyrC_C
Cuts at: 0 89 809 922 2200 2475 3339 3534
Size: 89 720 113 1278 275 864 195
Fragments arranged by size:
1278 864 720 275 195 113 89
BanII G_rGCy'C
Cuts at: 0 1338 1491 1889 1898 2436 2523 3239 3534
Size: 1338 153 398 9 538 87 716 295
Fragments arranged by size:
1338 716 538 398 295 153 87 9
BbeI G_GCGC'C
Cuts at: 0 93 3343 3534
Size: 93 3250 191
Fragments arranged by size:
3250 191 93
BbsI GAAGACnn'nnnn_
Cuts at: 0 1372 1968 2010 3219 3534
Size: 1372 596 42 1209 315
Fragments arranged by size:
1372 1209 596 315 42
BbvCI CC'TCA_GC
Cuts at: 0 1055 3534
Size: 1055 2479
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 3031 3065 3534
Size: 3031 34 469
Fragments arranged by size:
3031 469 34
BglI GCCn_nnn'nGGC
Cuts at: 0 2350 2440 3391 3534
Size: 2350 90 951 143
Fragments arranged by size:
2350 951 143 90
BglII A'GATC_T
Cuts at: 0 743 3534
Size: 743 2791
Bme1580I G_kGCm'C
Cuts at: 0 344 623 1338 1491 1598 1867 2361 2436
Size: 344 279 715 153 107 269 494 75
Cuts at: 2436 2480 3534
Size: 44 1054
Fragments arranged by size:
1054 715 494 344 279 269 153 107
75 44
BmgBI CAC'GTC
Cuts at: 0 524 2127 2324 2580 3321 3534
Size: 524 1603 197 256 741 213
Fragments arranged by size:
1603 741 524 256 213 197
BmrI ACTGGGnnnn_n'
Cuts at: 0 1428 1827 2520 2748 3534
Size: 1428 399 693 228 786
Fragments arranged by size:
1428 786 693 399 228
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 993 2959 3534
Size: 993 1966 575
Fragments arranged by size:
1966 993 575
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 115 658 2387 2954 2989 3175 3534
Size: 115 543 1729 567 35 186 359
Fragments arranged by size:
1729 567 543 359 186 115 35
Bpu10I CC'TnA_GC
Cuts at: 0 1055 2798 3534
Size: 1055 1743 736
Fragments arranged by size:
1743 1055 736
BsaAI yAC'GTr
Cuts at: 0 1050 3534
Size: 1050 2484
BsaBI GATnn'nnATC
Cuts at: 0 145 3364 3534
Size: 145 3219 170
Fragments arranged by size:
3219 170 145
BsaHI Gr'CG_yC
Cuts at: 0 90 1715 1838 2722 3007 3340 3534
Size: 90 1625 123 884 285 333 194
Fragments arranged by size:
1625 884 333 285 194 123 90
BsaWI w'CCGG_w
Cuts at: 0 407 1190 3534
Size: 407 783 2344
Fragments arranged by size:
2344 783 407
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 440 470 532 562 1252 1282 2081 2111
Size: 440 30 62 30 690 30 799 30
Cuts at: 2111 2360 2390 2476 2506 3534
Size: 249 30 86 30 1028
Fragments arranged by size:
1028 799 690 440 249 86 62 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 646 655 1024 1838 2718 3013 3151 3534
Size: 646 9 369 814 880 295 138 383
Fragments arranged by size:
880 814 646 383 369 295 138 9
BseYI C'CCAG_C
Cuts at: 0 174 1564 2061 2137 2422 2700 3534
Size: 174 1390 497 76 285 278 834
Fragments arranged by size:
1390 834 497 285 278 174 76
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 150 274 298 757 760 1460 2238 2287
Size: 150 124 24 459 3 700 778 49
Cuts at: 2287 2532 2605 2785 3157 3534
Size: 245 73 180 372 377
Fragments arranged by size:
778 700 459 377 372 245 180 150
124 73 49 24 3
BsiEI CG_ry'CG
Cuts at: 0 261 1176 1316 3534
Size: 261 915 140 2218
Fragments arranged by size:
2218 915 261 140
BsiHKAI G_wGCw'C
Cuts at: 0 344 623 998 1397 1889 1898 3023 3239
Size: 344 279 375 399 492 9 1125 216
Cuts at: 3239 3419 3534
Size: 180 115
Fragments arranged by size:
1125 492 399 375 344 279 216 180
115 9
Bsp1286I G_dGCh'C
Cuts at: 0 344 623 998 1338 1397 1491 1598 1867
Size: 344 279 375 340 59 94 107 269
Cuts at: 1867 1889 1898 2361 2436 2480 2523 3023 3239
Size: 22 9 463 75 44 43 500 216
Cuts at: 3239 3419 3534
Size: 180 115
Fragments arranged by size:
500 463 375 344 340 279 269 216
180 115 107 94 75 59 44 43
22 9
BspEI T'CCGG_A
Cuts at: 0 1190 3534
Size: 1190 2344
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 296 978 2627 2918 3213 3534
Size: 296 682 1649 291 295 321
Fragments arranged by size:
1649 682 321 296 295 291
BsrBI CCG'CTC
Cuts at: 0 373 1196 2825 3159 3534
Size: 373 823 1629 334 375
Fragments arranged by size:
1629 823 375 373 334
BsrDI GCAATG_nn'
Cuts at: 0 476 1286 3534
Size: 476 810 2248
Fragments arranged by size:
2248 810 476
BsrFI r'CCGG_y
Cuts at: 0 227 1171 2987 3211 3534
Size: 227 944 1816 224 323
Fragments arranged by size:
1816 944 323 227 224
BssHII G'CGCG_C
Cuts at: 0 1150 3534
Size: 1150 2384
BssSI C'ACGA_G
Cuts at: 0 541 1739 3534
Size: 541 1198 1795
Fragments arranged by size:
1795 1198 541
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1392 2083 3534
Size: 1392 691 1451
Fragments arranged by size:
1451 1392 691
BstEII G'GTnAC_C
Cuts at: 0 1131 3444 3534
Size: 1131 2313 90
Fragments arranged by size:
2313 1131 90
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1944 2739 3432 3534
Size: 1944 795 693 102
Fragments arranged by size:
1944 795 693 102
BstYI r'GATC_y
Cuts at: 0 743 1043 2594 2955 3534
Size: 743 300 1551 361 579
Fragments arranged by size:
1551 743 579 361 300
Bsu36I CC'TnA_GG
Cuts at: 0 31 1649 3534
Size: 31 1618 1885
Fragments arranged by size:
1885 1618 31
BtgI C'CryG_G
Cuts at: 0 21 261 724 926 1293 1310 1571 2246
Size: 21 240 463 202 367 17 261 675
Cuts at: 2246 2480 2828 2865 3455 3534
Size: 234 348 37 590 79
Fragments arranged by size:
675 590 463 367 348 261 240 234
202 79 37 21 17
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 456 1359 3534
Size: 456 903 2175
Fragments arranged by size:
2175 903 456
BtsI GCAGTG_nn'
Cuts at: 0 344 1598 1726 2155 2299 3068 3534
Size: 344 1254 128 429 144 769 466
Fragments arranged by size:
1254 769 466 429 344 144 128
DrdI GACnn_nn'nnGTC
Cuts at: 0 1582 1972 3534
Size: 1582 390 1562
Fragments arranged by size:
1582 1562 390
EaeI y'GGCC_r
Cuts at: 0 125 258 314 674 1173 1313 1512 1733
Size: 125 133 56 360 499 140 199 221
Cuts at: 1733 1994 2129 2177 2228 2552 3188 3213 3392
Size: 261 135 48 51 324 636 25 179
Cuts at: 3392 3534
Size: 142
Fragments arranged by size:
636 499 360 324 261 221 199 179
142 140 135 133 125 56 51 48
25
EagI C'GGCC_G
Cuts at: 0 258 1173 1313 3534
Size: 258 915 140 2221
Fragments arranged by size:
2221 915 258 140
EarI CTCTTCn'nnn_
Cuts at: 0 1807 2177 2801 3115 3534
Size: 1807 370 624 314 419
Fragments arranged by size:
1807 624 419 370 314
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 37 1204 1221 2818 3534
Size: 37 1167 17 1597 716
Fragments arranged by size:
1597 1167 716 37 17
EcoICRI GAG'CTC
Cuts at: 0 1887 1896 3237 3534
Size: 1887 9 1341 297
Fragments arranged by size:
1887 1341 297 9
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 115 658 1692 1776 1788 2260 2387
Size: 25 90 543 1034 84 12 472 127
Cuts at: 2387 2923 2954 2989 3175 3534
Size: 536 31 35 186 359
Fragments arranged by size:
1034 543 536 472 359 186 127 90
84 35 31 25 12
EcoNI CCTnn'n_nnAGG
Cuts at: 0 532 1087 3534
Size: 532 555 2447
Fragments arranged by size:
2447 555 532
EcoO109I rG'GnC_Cy
Cuts at: 0 27 1143 1334 1335 1645 2432 2433 2745
Size: 27 1116 191 1 310 787 1 312
Cuts at: 2745 2907 3121 3309 3534
Size: 162 214 188 225
Fragments arranged by size:
1116 787 312 310 225 214 191 188
162 27 1 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 978 3534
Size: 978 2556
FseI GG_CCGG'CC
Cuts at: 0 3215 3534
Size: 3215 319
FspI TGC'GCA
Cuts at: 0 1747 3534
Size: 1747 1787
FspAI rTGC'GCAy
Cuts at: 0 1747 3534
Size: 1747 1787
HaeII r_GCGC'y
Cuts at: 0 93 697 3343 3534
Size: 93 604 2646 191
Fragments arranged by size:
2646 604 191 93
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1235 1267 2386 2418 3007 3039 3113 3145
Size: 1235 32 1119 32 589 32 74 32
Cuts at: 3145 3534
Size: 389
Fragments arranged by size:
1235 1119 589 389 74 32 32 32
32
HincII GTy'rAC
Cuts at: 0 975 3534
Size: 975 2559
KasI G'GCGC_C
Cuts at: 0 89 3339 3534
Size: 89 3250 195
Fragments arranged by size:
3250 195 89
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 575 1558 3534
Size: 575 983 1976
Fragments arranged by size:
1976 983 575
MscI TGG'CCA
Cuts at: 0 127 316 1735 1996 3394 3534
Size: 127 189 1419 261 1398 140
Fragments arranged by size:
1419 1398 261 189 140 127
MslI CAynn'nnrTG
Cuts at: 0 151 310 613 736 1183 1324 1741 2473
Size: 151 159 303 123 447 141 417 732
Cuts at: 2473 2716 3370 3430 3534
Size: 243 654 60 104
Fragments arranged by size:
732 654 447 417 303 243 159 151
141 123 104 60
MspA1I CmG'CkG
Cuts at: 0 70 263 804 1467 2392 3249 3534
Size: 70 193 541 663 925 857 285
Fragments arranged by size:
925 857 663 541 285 193 70
NaeI GCC'GGC
Cuts at: 0 3213 3534
Size: 3213 321
NarI GG'CG_CC
Cuts at: 0 90 3340 3534
Size: 90 3250 194
Fragments arranged by size:
3250 194 90
NcoI C'CATG_G
Cuts at: 0 21 2480 2865 3455 3534
Size: 21 2459 385 590 79
Fragments arranged by size:
2459 590 385 79 21
NgoMIV G'CCGG_C
Cuts at: 0 3211 3534
Size: 3211 323
NspI r_CATG'y
Cuts at: 0 2058 3534
Size: 2058 1476
PasI CC'CwG_GG
Cuts at: 0 2599 3534
Size: 2599 935
PflMI CCAn_nnn'nTGG
Cuts at: 0 819 1521 2640 2700 2760 3534
Size: 819 702 1119 60 60 774
Fragments arranged by size:
1119 819 774 702 60 60
PfoI T'CCnGG_A
Cuts at: 0 1889 2276 3521 3534
Size: 1889 387 1245 13
Fragments arranged by size:
1889 1245 387 13
PmlI CAC'GTG
Cuts at: 0 1050 3534
Size: 1050 2484
PpuMI rG'GwC_Cy
Cuts at: 0 1143 2745 2907 3121 3534
Size: 1143 1602 162 214 413
Fragments arranged by size:
1602 1143 413 214 162
PspOMI G'GGCC_C
Cuts at: 0 1334 1487 2432 3534
Size: 1334 153 945 1102
Fragments arranged by size:
1334 1102 945 153
PspXI vC'TCGA_Gb
Cuts at: 0 1661 3534
Size: 1661 1873
PstI C_TGCA'G
Cuts at: 0 989 1388 1593 1883 2162 2846 2963 3119
Size: 989 399 205 290 279 684 117 156
Cuts at: 3119 3534
Size: 415
Fragments arranged by size:
989 684 415 399 290 279 205 156
117
PvuII CAG'CTG
Cuts at: 0 1467 2392 3249 3534
Size: 1467 925 857 285
Fragments arranged by size:
1467 925 857 285
SacI G_AGCT'C
Cuts at: 0 1889 1898 3239 3534
Size: 1889 9 1341 295
Fragments arranged by size:
1889 1341 295 9
SacII CC_GC'GG
Cuts at: 0 264 3534
Size: 264 3270
SanDI GG'GwC_CC
Cuts at: 0 3121 3534
Size: 3121 413
SapI GCTCTTCn'nnn_
Cuts at: 0 1807 2177 2801 3534
Size: 1807 370 624 733
Fragments arranged by size:
1807 733 624 370
SbfI CC_TGCA'GG
Cuts at: 0 989 2846 3119 3534
Size: 989 1857 273 415
Fragments arranged by size:
1857 989 415 273
ScaI AGT'ACT
Cuts at: 0 2152 2164 2509 3534
Size: 2152 12 345 1025
Fragments arranged by size:
2152 1025 345 12
SexAI A'CCwGG_T
Cuts at: 0 1028 3534
Size: 1028 2506
SfcI C'TryA_G
Cuts at: 0 985 1384 1589 1879 2158 2499 2842 2959
Size: 985 399 205 290 279 341 343 117
Cuts at: 2959 3115 3534
Size: 156 419
Fragments arranged by size:
985 419 399 343 341 290 279 205
156 117
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 2440 3391 3534
Size: 2440 951 143
Fragments arranged by size:
2440 951 143
SfoI GGC'GCC
Cuts at: 0 91 3341 3534
Size: 91 3250 193
Fragments arranged by size:
3250 193 91
SmaI CCC'GGG
Cuts at: 0 466 655 1682 3534
Size: 466 189 1027 1852
Fragments arranged by size:
1852 1027 466 189
SmlI C'TyrA_G
Cuts at: 0 1661 3534
Size: 1661 1873
SrfI GCCC'GGGC
Cuts at: 0 466 3534
Size: 466 3068
StuI AGG'CCT
Cuts at: 0 283 388 2677 3534
Size: 283 105 2289 857
Fragments arranged by size:
2289 857 283 105
StyI C'CwwG_G
Cuts at: 0 21 317 1239 2480 2865 2914 3305 3386
Size: 21 296 922 1241 385 49 391 81
Cuts at: 3386 3395 3455 3488 3534
Size: 9 60 33 46
Fragments arranged by size:
1241 922 391 385 296 81 60 49
46 33 21 9
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1447 1765 2218 2329 3289 3468 3534
Size: 1447 318 453 111 960 179 66
Fragments arranged by size:
1447 960 453 318 179 111 66
TatI w'GTAC_w
Cuts at: 0 2150 2162 2507 2609 3534
Size: 2150 12 345 102 925
Fragments arranged by size:
2150 925 345 102 12
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 440 472 532 564 1252 1284 1530 1562
Size: 440 32 60 32 688 32 246 32
Cuts at: 1562 2081 2113 2291 2323 2360 2392 3534
Size: 519 32 178 32 37 32 1142
Fragments arranged by size:
1142 688 519 440 246 178 60 37
32 32 32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1030 3534
Size: 1030 2504
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2058 2697 3534
Size: 2058 639 837
Fragments arranged by size:
2058 837 639
XhoI C'TCGA_G
Cuts at: 0 1661 3534
Size: 1661 1873
XmaI C'CCGG_G
Cuts at: 0 464 653 1680 3534
Size: 464 189 1027 1854
Fragments arranged by size:
1854 1027 464 189
XmnI GAAnn'nnTTC
Cuts at: 0 2885 3534
Size: 2885 649
ZraI GAC'GTC
Cuts at: 0 1839 3534
Size: 1839 1695
Enzymes that do cut:
AarI AatII AccI AcuI AflIII AleI AlfI AloI
AlwNI ApaI ApaLI AvaI BanI BanII BbeI BbsI
BbvCI BcgI BglI BglII Bme1580I BmgBI BmrI BplI
BpmI Bpu10I BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrDI BsrFI BssHII BssSI BstAPI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI DrdI EaeI EagI EarI
EciI EcoICRI Eco57MI EcoNI EcoO109I FalI FseI FspI
FspAI HaeII Hin4I HincII KasI MmeI MscI MslI
MspA1I NaeI NarI NcoI NgoMIV NspI PasI PflMI
PfoI PmlI PpuMI PspOMI PspXI PstI PvuII SacI
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SmaI SmlI SrfI StuI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
Acc65I AclI AfeI AflII AgeI AhdI ApoI AscI
AseI AsiSI AvrII BaeI BamHI BciVI BclI BfrBI
BlpI BmtI BpuEI BsaI BsiWI BsmI BsmBI BspHI
BsrGI BstBI BstZ17I ClaI CspCI DraI DraIII EcoRI
EcoRV HindIII HpaI KpnI MfeI MluI NdeI NheI
NotI NruI NsiI PacI PciI PmeI PpiI PshAI
PsiI PsrI PvuI RsrII SalI SgfI SgrAI SnaBI
SpeI SphI SspI SwaI XbaI