Restriction map for
pF1KB3702
(Linear) (Six-base) MAPSORT of: KIBB3702.seq Check: 8442 from: 1 to: 3114
KIBB3702.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 202 3114
Size: 202 2912
AccI GT'mk_AC
Cuts at: 0 1211 1564 2859 3114
Size: 1211 353 1295 255
Fragments arranged by size:
1295 1211 353 255
AflII C'TTAA_G
Cuts at: 0 2941 3114
Size: 2941 173
AflIII A'CryG_T
Cuts at: 0 1214 2919 3114
Size: 1214 1705 195
Fragments arranged by size:
1705 1214 195
AleI CACnn'nnGTG
Cuts at: 0 884 3114
Size: 884 2230
AlwNI CAG_nnn'CTG
Cuts at: 0 475 682 2779 3114
Size: 475 207 2097 335
Fragments arranged by size:
2097 475 335 207
ApaI G_GGCC'C
Cuts at: 0 2529 3114
Size: 2529 585
ApoI r'AATT_y
Cuts at: 0 387 714 1752 2289 2314 3114
Size: 387 327 1038 537 25 800
Fragments arranged by size:
1038 800 537 387 327 25
AseI AT'TA_AT
Cuts at: 0 2579 3114
Size: 2579 535
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 1276 1309 3114
Size: 1276 33 1805
Fragments arranged by size:
1805 1276 33
BanI G'GyrC_C
Cuts at: 0 30 1149 2951 3114
Size: 30 1119 1802 163
Fragments arranged by size:
1802 1119 163 30
BanII G_rGCy'C
Cuts at: 0 1546 2306 2529 3114
Size: 1546 760 223 585
Fragments arranged by size:
1546 760 585 223
BbeI G_GCGC'C
Cuts at: 0 2955 3114
Size: 2955 159
BbsI GAAGACnn'nnnn_
Cuts at: 0 538 1448 1664 1988 3114
Size: 538 910 216 324 1126
Fragments arranged by size:
1126 910 538 324 216
BbvCI CC'TCA_GC
Cuts at: 0 1490 3114
Size: 1490 1624
BciVI GTATCCnnnnn_n'
Cuts at: 0 505 3114
Size: 505 2609
BglII A'GATC_T
Cuts at: 0 2222 3114
Size: 2222 892
Bme1580I G_kGCm'C
Cuts at: 0 35 2529 3114
Size: 35 2494 585
Fragments arranged by size:
2494 585 35
BmrI ACTGGGnnnn_n'
Cuts at: 0 900 3114
Size: 900 2214
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2378 3114
Size: 2378 736
Bpu10I CC'TnA_GC
Cuts at: 0 1490 3114
Size: 1490 1624
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1200 1411 2014 3114
Size: 1200 211 603 1100
Fragments arranged by size:
1200 1100 603 211
BsaI GGTCTCn'nnnn_
Cuts at: 0 260 524 1368 2253 3114
Size: 260 264 844 885 861
Fragments arranged by size:
885 861 844 264 260
BsaBI GATnn'nnATC
Cuts at: 0 116 2048 3114
Size: 116 1932 1066
Fragments arranged by size:
1932 1066 116
BsaHI Gr'CG_yC
Cuts at: 0 2952 3114
Size: 2952 162
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 294 297 324 327 3114
Size: 294 3 27 3 2787
Fragments arranged by size:
2787 294 27 3 3
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 302 977 1635 3114
Size: 302 675 658 1479
Fragments arranged by size:
1479 675 658 302
BseYI C'CCAG_C
Cuts at: 0 1795 3114
Size: 1795 1319
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1463 3114
Size: 1463 1651
BsiHKAI G_wGCw'C
Cuts at: 0 2063 2805 3114
Size: 2063 742 309
Fragments arranged by size:
2063 742 309
BsmI GAATG_Cn'
Cuts at: 0 1707 3114
Size: 1707 1407
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2607 3114
Size: 2607 507
Bsp1286I G_dGCh'C
Cuts at: 0 35 1546 2063 2306 2529 2805 3114
Size: 35 1511 517 243 223 276 309
Fragments arranged by size:
1511 517 309 276 243 223 35
BspHI T'CATG_A
Cuts at: 0 156 3114
Size: 156 2958
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 202 987 1338 2419 3114
Size: 202 785 351 1081 695
Fragments arranged by size:
1081 785 695 351 202
BsrDI GCAATG_nn'
Cuts at: 0 455 767 3114
Size: 455 312 2347
Fragments arranged by size:
2347 455 312
BsrGI T'GTAC_A
Cuts at: 0 1258 3114
Size: 1258 1856
BssSI C'ACGA_G
Cuts at: 0 959 1354 3114
Size: 959 395 1760
Fragments arranged by size:
1760 959 395
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1486 3114
Size: 1486 1628
BstXI CCAn_nnnn'nTGG
Cuts at: 0 57 1394 3114
Size: 57 1337 1720
Fragments arranged by size:
1720 1337 57
BstYI r'GATC_y
Cuts at: 0 111 267 853 2222 2775 2823 3114
Size: 111 156 586 1369 553 48 291
Fragments arranged by size:
1369 586 553 291 156 111 48
BstZ17I GTA'TAC
Cuts at: 0 2860 3114
Size: 2860 254
BtgI C'CryG_G
Cuts at: 0 43 3080 3114
Size: 43 3037 34
Fragments arranged by size:
3037 43 34
BtsI GCAGTG_nn'
Cuts at: 0 65 800 1421 3041 3068 3114
Size: 65 735 621 1620 27 46
Fragments arranged by size:
1620 735 621 65 46 27
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1914 1949 3114
Size: 1914 35 1165
Fragments arranged by size:
1914 1165 35
DraI TTT'AAA
Cuts at: 0 108 1001 3114
Size: 108 893 2113
Fragments arranged by size:
2113 893 108
DraIII CAC_nnn'GTG
Cuts at: 0 1486 2755 3114
Size: 1486 1269 359
Fragments arranged by size:
1486 1269 359
DrdI GACnn_nn'nnGTC
Cuts at: 0 2995 3114
Size: 2995 119
EaeI y'GGCC_r
Cuts at: 0 627 2184 2496 2631 2677 2691 3114
Size: 627 1557 312 135 46 14 423
Fragments arranged by size:
1557 627 423 312 135 46 14
EarI CTCTTCn'nnn_
Cuts at: 0 1273 2793 3114
Size: 1273 1520 321
Fragments arranged by size:
1520 1273 321
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2378 3114
Size: 2378 736
EcoO109I rG'GnC_Cy
Cuts at: 0 2525 3114
Size: 2525 589
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 2761 3114
Size: 2761 353
HaeII r_GCGC'y
Cuts at: 0 2955 3114
Size: 2955 159
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1177 1209 2214 2246 2875 2907 3114
Size: 1177 32 1005 32 629 32 207
Fragments arranged by size:
1177 1005 629 207 32 32 32
HincII GTy'rAC
Cuts at: 0 375 524 2448 2876 3000 3114
Size: 375 149 1924 428 124 114
Fragments arranged by size:
1924 428 375 149 124 114
HpaI GTT'AAC
Cuts at: 0 2448 3114
Size: 2448 666
KasI G'GCGC_C
Cuts at: 0 2951 3114
Size: 2951 163
MfeI C'AATT_G
Cuts at: 0 1943 3114
Size: 1943 1171
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 596 765 1299 3114
Size: 596 169 534 1815
Fragments arranged by size:
1815 596 534 169
MscI TGG'CCA
Cuts at: 0 2186 2498 2633 3114
Size: 2186 312 135 481
Fragments arranged by size:
2186 481 312 135
MslI CAynn'nnrTG
Cuts at: 0 161 884 3114
Size: 161 723 2230
Fragments arranged by size:
2230 723 161
MspA1I CmG'CkG
Cuts at: 0 18 1971 3114
Size: 18 1953 1143
Fragments arranged by size:
1953 1143 18
NarI GG'CG_CC
Cuts at: 0 2952 3114
Size: 2952 162
NcoI C'CATG_G
Cuts at: 0 43 3080 3114
Size: 43 3037 34
Fragments arranged by size:
3037 43 34
NspI r_CATG'y
Cuts at: 0 1218 2195 3114
Size: 1218 977 919
Fragments arranged by size:
1218 977 919
PasI CC'CwG_GG
Cuts at: 0 1096 1273 3114
Size: 1096 177 1841
Fragments arranged by size:
1841 1096 177
PciI A'CATG_T
Cuts at: 0 1214 3114
Size: 1214 1900
PflMI CCAn_nnn'nTGG
Cuts at: 0 1618 1870 3114
Size: 1618 252 1244
Fragments arranged by size:
1618 1244 252
PfoI T'CCnGG_A
Cuts at: 0 2843 3114
Size: 2843 271
PsiI TTA'TAA
Cuts at: 0 974 3114
Size: 974 2140
PspOMI G'GGCC_C
Cuts at: 0 2525 3114
Size: 2525 589
PstI C_TGCA'G
Cuts at: 0 17 807 3114
Size: 17 790 2307
Fragments arranged by size:
2307 790 17
PvuII CAG'CTG
Cuts at: 0 1971 3114
Size: 1971 1143
SapI GCTCTTCn'nnn_
Cuts at: 0 2793 3114
Size: 2793 321
ScaI AGT'ACT
Cuts at: 0 841 1869 2103 2489 3114
Size: 841 1028 234 386 625
Fragments arranged by size:
1028 841 625 386 234
SfcI C'TryA_G
Cuts at: 0 13 803 1733 3114
Size: 13 790 930 1381
Fragments arranged by size:
1381 930 790 13
SfoI GGC'GCC
Cuts at: 0 2953 3114
Size: 2953 161
SmlI C'TyrA_G
Cuts at: 0 1179 1426 2029 2941 3114
Size: 1179 247 603 912 173
Fragments arranged by size:
1179 912 603 247 173
SpeI A'CTAG_T
Cuts at: 0 2104 2266 3114
Size: 2104 162 848
Fragments arranged by size:
2104 848 162
SspI AAT'ATT
Cuts at: 0 173 823 1695 2577 3114
Size: 173 650 872 882 537
Fragments arranged by size:
882 872 650 537 173
StuI AGG'CCT
Cuts at: 0 2811 3114
Size: 2811 303
StyI C'CwwG_G
Cuts at: 0 43 129 662 3080 3114
Size: 43 86 533 2418 34
Fragments arranged by size:
2418 533 86 43 34
TatI w'GTAC_w
Cuts at: 0 839 1258 1867 2101 2487 3114
Size: 839 419 609 234 386 627
Fragments arranged by size:
839 627 609 419 386 234
XbaI T'CTAG_A
Cuts at: 0 597 3114
Size: 597 2517
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2421 2476 2841 2982 3114
Size: 2421 55 365 141 132
Fragments arranged by size:
2421 365 141 132 55
XmnI GAAnn'nnTTC
Cuts at: 0 2645 2700 3114
Size: 2645 55 414
Fragments arranged by size:
2645 414 55
Enzymes that do cut:
AarI AccI AflII AflIII AleI AlwNI ApaI ApoI
AseI BaeI BanI BanII BbeI BbsI BbvCI BciVI
BglII Bme1580I BmrI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI
Bsp1286I BspHI BspMI BsrDI BsrGI BssSI BstAPI BstXI
BstYI BstZ17I BtgI BtsI CspCI DraI DraIII DrdI
EaeI EarI Eco57MI EcoO109I FalI HaeII Hin4I HincII
HpaI KasI MfeI MmeI MscI MslI MspA1I NarI
NcoI NspI PasI PciI PflMI PfoI PsiI PspOMI
PstI PvuII SapI ScaI SfcI SfoI SmlI SpeI
SspI StuI StyI TatI XbaI XcmI XmnI
Enzymes that do not cut:
AatII Acc65I AclI AcuI AfeI AgeI AhdI AlfI
AloI ApaLI AscI AsiSI AvaI AvrII BamHI BcgI
BclI BfrBI BglI BlpI BmgBI BmtI BplI BsaAI
BsaWI BsiEI BsiWI BspEI BsrBI BsrFI BssHII BstBI
BstEII Bsu36I BtgZI ClaI EagI EciI EcoICRI EcoNI
EcoRI EcoRV FseI FspI FspAI HindIII KpnI MluI
NaeI NdeI NgoMIV NheI NotI NruI NsiI PacI
PmeI PmlI PpiI PpuMI PshAI PspXI PsrI PvuI
RsrII SacI SacII SalI SanDI SbfI SexAI SfiI
SgfI SgrAI SmaI SnaBI SphI SrfI SwaI TaqII
TstI Tth111I XhoI XmaI ZraI