Restriction map for
pF1KB3707
(Linear) (Six-base) MAPSORT of: KIBB3707.seq Check: 7184 from: 1 to: 2997
KIBB3707.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1125 1845 2997
Size: 1125 720 1152
Fragments arranged by size:
1152 1125 720
AccI GT'mk_AC
Cuts at: 0 1325 2997
Size: 1325 1672
Acc65I G'GTAC_C
Cuts at: 0 1762 2997
Size: 1762 1235
AclI AA'CG_TT
Cuts at: 0 2566 2997
Size: 2566 431
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1210 1405 1535 2152 2864 2997
Size: 503 707 195 130 617 712 133
Fragments arranged by size:
712 707 617 503 195 133 130
AgeI A'CCGG_T
Cuts at: 0 204 2997
Size: 204 2793
AhdI GACnn_n'nnGTC
Cuts at: 0 1602 2997
Size: 1602 1395
AleI CACnn'nnGTG
Cuts at: 0 724 2371 2551 2997
Size: 724 1647 180 446
Fragments arranged by size:
1647 724 446 180
AlwNI CAG_nnn'CTG
Cuts at: 0 1545 2020 2712 2869 2941 2997
Size: 1545 475 692 157 72 56
Fragments arranged by size:
1545 692 475 157 72 56
ApaI G_GGCC'C
Cuts at: 0 1424 2517 2997
Size: 1424 1093 480
Fragments arranged by size:
1424 1093 480
ApaLI G'TGCA_C
Cuts at: 0 400 2997
Size: 400 2597
ApoI r'AATT_y
Cuts at: 0 1039 2997
Size: 1039 1958
AvaI C'yCGr_G
Cuts at: 0 1160 2269 2771 2997
Size: 1160 1109 502 226
Fragments arranged by size:
1160 1109 502 226
BamHI G'GATC_C
Cuts at: 0 2887 2997
Size: 2887 110
BanI G'GyrC_C
Cuts at: 0 1074 1762 2997
Size: 1074 688 1235
Fragments arranged by size:
1235 1074 688
BanII G_rGCy'C
Cuts at: 0 703 830 1424 1822 1865 2517 2858 2997
Size: 703 127 594 398 43 652 341 139
Fragments arranged by size:
703 652 594 398 341 139 127 43
BbsI GAAGACnn'nnnn_
Cuts at: 0 525 636 751 2997
Size: 525 111 115 2246
Fragments arranged by size:
2246 525 115 111
BbvCI CC'TCA_GC
Cuts at: 0 631 1877 2401 2997
Size: 631 1246 524 596
Fragments arranged by size:
1246 631 596 524
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 556 590 2997
Size: 556 34 2407
Fragments arranged by size:
2407 556 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 1601 2997
Size: 1601 1396
BclI T'GATC_A
Cuts at: 0 518 2997
Size: 518 2479
BglI GCCn_nnn'nGGC
Cuts at: 0 33 55 1252 2460 2623 2997
Size: 33 22 1197 1208 163 374
Fragments arranged by size:
1208 1197 374 163 33 22
BglII A'GATC_T
Cuts at: 0 1518 2309 2540 2997
Size: 1518 791 231 457
Fragments arranged by size:
1518 791 457 231
BlpI GC'TnA_GC
Cuts at: 0 1276 2323 2396 2627 2997
Size: 1276 1047 73 231 370
Fragments arranged by size:
1276 1047 370 231 73
Bme1580I G_kGCm'C
Cuts at: 0 164 404 1424 2517 2997
Size: 164 240 1020 1093 480
Fragments arranged by size:
1093 1020 480 240 164
BmrI ACTGGGnnnn_n'
Cuts at: 0 1879 2997
Size: 1879 1118
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1914 2328 2997
Size: 1914 414 669
Fragments arranged by size:
1914 669 414
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 838 1084 2018 2212 2365 2813 2951
Size: 176 662 246 934 194 153 448 138
Cuts at: 2951 2997
Size: 46
Fragments arranged by size:
934 662 448 246 194 176 153 138
46
Bpu10I CC'TnA_GC
Cuts at: 0 631 702 1877 2401 2997
Size: 631 71 1175 524 596
Fragments arranged by size:
1175 631 596 524 71
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1849 2491 2997
Size: 1849 642 506
Fragments arranged by size:
1849 642 506
BsaI GGTCTCn'nnnn_
Cuts at: 0 2180 2239 2581 2997
Size: 2180 59 342 416
Fragments arranged by size:
2180 416 342 59
BsaHI Gr'CG_yC
Cuts at: 0 2027 2997
Size: 2027 970
BsaWI w'CCGG_w
Cuts at: 0 204 2997
Size: 204 2793
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 45 1060 1222 1375 1378 1828 2104 2308
Size: 45 1015 162 153 3 450 276 204
Cuts at: 2308 2320 2452 2473 2997
Size: 12 132 21 524
Fragments arranged by size:
1015 524 450 276 204 162 153 132
45 21 12 3
BseYI C'CCAG_C
Cuts at: 0 757 850 1212 2997
Size: 757 93 362 1785
Fragments arranged by size:
1785 757 362 93
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 272 1076 2179 2725 2997
Size: 272 804 1103 546 272
Fragments arranged by size:
1103 804 546 272 272
BsiHKAI G_wGCw'C
Cuts at: 0 65 368 404 830 1652 1865 2997
Size: 65 303 36 426 822 213 1132
Fragments arranged by size:
1132 822 426 303 213 65 36
BsmI GAATG_Cn'
Cuts at: 0 2610 2997
Size: 2610 387
Bsp1286I G_dGCh'C
Cuts at: 0 65 164 368 404 703 830 1424 1652
Size: 65 99 204 36 299 127 594 228
Cuts at: 1652 1822 1865 2517 2858 2997
Size: 170 43 652 341 139
Fragments arranged by size:
652 594 341 299 228 204 170 139
127 99 65 43 36
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1125 1845 2129 2997
Size: 1125 720 284 868
Fragments arranged by size:
1125 868 720 284
BsrBI CCG'CTC
Cuts at: 0 1071 2997
Size: 1071 1926
BsrFI r'CCGG_y
Cuts at: 0 204 2997
Size: 204 2793
BsrGI T'GTAC_A
Cuts at: 0 1086 2997
Size: 1086 1911
BssSI C'ACGA_G
Cuts at: 0 367 1031 1347 2997
Size: 367 664 316 1650
Fragments arranged by size:
1650 664 367 316
BstAPI GCAn_nnn'nTGC
Cuts at: 0 399 716 2712 2997
Size: 399 317 1996 285
Fragments arranged by size:
1996 399 317 285
BstXI CCAn_nnnn'nTGG
Cuts at: 0 138 1338 2226 2343 2997
Size: 138 1200 888 117 654
Fragments arranged by size:
1200 888 654 138 117
BstYI r'GATC_y
Cuts at: 0 779 1518 2309 2540 2887 2997
Size: 779 739 791 231 347 110
Fragments arranged by size:
791 779 739 347 231 110
Bsu36I CC'TnA_GG
Cuts at: 0 437 1922 1940 2021 2753 2997
Size: 437 1485 18 81 732 244
Fragments arranged by size:
1485 732 437 244 81 18
BtgI C'CryG_G
Cuts at: 0 164 914 1196 1247 2997
Size: 164 750 282 51 1750
Fragments arranged by size:
1750 750 282 164 51
BtsI GCAGTG_nn'
Cuts at: 0 107 129 521 623 668 1262 1325 2997
Size: 107 22 392 102 45 594 63 1672
Fragments arranged by size:
1672 594 392 107 102 63 45 22
DraI TTT'AAA
Cuts at: 0 1290 2997
Size: 1290 1707
DraIII CAC_nnn'GTG
Cuts at: 0 88 2997
Size: 88 2909
EaeI y'GGCC_r
Cuts at: 0 140 917 2615 2997
Size: 140 777 1698 382
Fragments arranged by size:
1698 777 382 140
EarI CTCTTCn'nnn_
Cuts at: 0 1411 1700 1805 2997
Size: 1411 289 105 1192
Fragments arranged by size:
1411 1192 289 105
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 637 2997
Size: 637 2360
EcoICRI GAG'CTC
Cuts at: 0 828 1863 2997
Size: 828 1035 1134
Fragments arranged by size:
1134 1035 828
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 176 503 838 1084 1210 1405 1535 2018
Size: 176 327 335 246 126 195 130 483
Cuts at: 2018 2152 2212 2365 2813 2864 2951 2997
Size: 134 60 153 448 51 87 46
Fragments arranged by size:
483 448 335 327 246 195 176 153
134 130 126 87 60 51 46
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2831 2997
Size: 2831 166
EcoO109I rG'GnC_Cy
Cuts at: 0 813 1095 1164 1420 1421 1510 1881 2017
Size: 813 282 69 256 1 89 371 136
Cuts at: 2017 2513 2768 2926 2997
Size: 496 255 158 71
Fragments arranged by size:
813 496 371 282 256 255 158 136
89 71 69 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1641 2997
Size: 1641 1356
HaeII r_GCGC'y
Cuts at: 0 555 2330 2997
Size: 555 1775 667
Fragments arranged by size:
1775 667 555
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 258 290 685 717 1588 1620 2872 2904
Size: 258 32 395 32 871 32 1252 32
Cuts at: 2904 2997
Size: 93
Fragments arranged by size:
1252 871 395 258 93 32 32 32
32
KpnI G_GTAC'C
Cuts at: 0 1766 2997
Size: 1766 1231
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 2340 2997
Size: 2340 657
MscI TGG'CCA
Cuts at: 0 142 919 2997
Size: 142 777 2078
Fragments arranged by size:
2078 777 142
MslI CAynn'nnrTG
Cuts at: 0 136 724 1336 2371 2374 2551 2997
Size: 136 588 612 1035 3 177 446
Fragments arranged by size:
1035 612 588 446 177 136 3
MspA1I CmG'CkG
Cuts at: 0 423 774 1270 2997
Size: 423 351 496 1727
Fragments arranged by size:
1727 496 423 351
NcoI C'CATG_G
Cuts at: 0 164 914 1247 2997
Size: 164 750 333 1750
Fragments arranged by size:
1750 750 333 164
NspI r_CATG'y
Cuts at: 0 2502 2997
Size: 2502 495
PasI CC'CwG_GG
Cuts at: 0 807 2997
Size: 807 2190
PfoI T'CCnGG_A
Cuts at: 0 359 1802 2928 2997
Size: 359 1443 1126 69
Fragments arranged by size:
1443 1126 359 69
PpuMI rG'GwC_Cy
Cuts at: 0 1164 2768 2926 2997
Size: 1164 1604 158 71
Fragments arranged by size:
1604 1164 158 71
PspOMI G'GGCC_C
Cuts at: 0 1420 2513 2997
Size: 1420 1093 484
Fragments arranged by size:
1420 1093 484
PstI C_TGCA'G
Cuts at: 0 428 569 602 779 1827 2997
Size: 428 141 33 177 1048 1170
Fragments arranged by size:
1170 1048 428 177 141 33
PvuII CAG'CTG
Cuts at: 0 423 774 1270 2997
Size: 423 351 496 1727
Fragments arranged by size:
1727 496 423 351
SacI G_AGCT'C
Cuts at: 0 830 1865 2997
Size: 830 1035 1132
Fragments arranged by size:
1132 1035 830
SanDI GG'GwC_CC
Cuts at: 0 1164 2768 2997
Size: 1164 1604 229
Fragments arranged by size:
1604 1164 229
SfcI C'TryA_G
Cuts at: 0 424 565 598 775 1458 1823 2997
Size: 424 141 33 177 683 365 1174
Fragments arranged by size:
1174 683 424 365 177 141 33
SgrAI Cr'CCGG_yG
Cuts at: 0 204 2997
Size: 204 2793
SmaI CCC'GGG
Cuts at: 0 2773 2997
Size: 2773 224
SmlI C'TyrA_G
Cuts at: 0 1864 2506 2997
Size: 1864 642 491
Fragments arranged by size:
1864 642 491
StuI AGG'CCT
Cuts at: 0 995 2997
Size: 995 2002
StyI C'CwwG_G
Cuts at: 0 50 164 914 1247 1607 1778 2415 2997
Size: 50 114 750 333 360 171 637 582
Fragments arranged by size:
750 637 582 360 333 171 114 50
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1138 1561 2711 2997
Size: 1138 423 1150 286
Fragments arranged by size:
1150 1138 423 286
TatI w'GTAC_w
Cuts at: 0 1086 2997
Size: 1086 1911
XbaI T'CTAG_A
Cuts at: 0 1656 2997
Size: 1656 1341
XmaI C'CCGG_G
Cuts at: 0 2771 2997
Size: 2771 226
XmnI GAAnn'nnTTC
Cuts at: 0 1520 2997
Size: 1520 1477
Enzymes that do cut:
AarI AccI Acc65I AclI AcuI AgeI AhdI AleI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI
Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI BsaHI
BsaWI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I BspMI
BsrBI BsrFI BsrGI BssSI BstAPI BstXI BstYI Bsu36I
BtgI BtsI DraI DraIII EaeI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII Hin4I KpnI MmeI
MscI MslI MspA1I NcoI NspI PasI PfoI PpuMI
PspOMI PstI PvuII SacI SanDI SfcI SgrAI SmaI
SmlI StuI StyI TaqII TatI XbaI XmaI XmnI
Enzymes that do not cut:
AatII AfeI AflII AflIII AlfI AloI AscI AseI
AsiSI AvrII BaeI BbeI BfrBI BmgBI BmtI BsaAI
BsaBI BsaXI BsiEI BsiWI BsmBI BspEI BspHI BsrDI
BssHII BstBI BstEII BstZ17I BtgZI ClaI CspCI DrdI
EagI EcoRI EcoRV FseI FspI FspAI HincII HindIII
HpaI KasI MfeI MluI NaeI NarI NdeI NgoMIV
NheI NotI NruI NsiI PacI PciI PflMI PmeI
PmlI PpiI PshAI PsiI PspXI PsrI PvuI RsrII
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SnaBI SpeI SphI SrfI SspI SwaI TstI
Tth111I XcmI XhoI ZraI