(Linear) (Six-base) MAPSORT of: KIBB3711.seq  Check: 8254  from: 1  to: 225



KIBB3711.orf

 With 182 enzymes: SgfI * 

                        March 3, 2008 10:56  ..

AlwNI CAG_nnn'CTG

Cuts at:      0     175     225 
   Size:        175      50
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     105     225 
   Size:        105     120
 
BsaAI yAC'GTr

Cuts at:      0     144     225 
   Size:        144      81
 
BstYI r'GATC_y

Cuts at:      0     153     225 
   Size:        153      72
 
BtgI C'CryG_G

Cuts at:      0     113     225 
   Size:        113     112
 
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'

Cuts at:      0     225 
   Size:        225
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0      30     225 
   Size:         30     195
 
MslI CAynn'nnrTG

Cuts at:      0     169     225 
   Size:        169      56
 
MspA1I CmG'CkG

Cuts at:      0     115     225 
   Size:        115     110
 
NruI TCG'CGA

Cuts at:      0     197     225 
   Size:        197      28
 
NspI r_CATG'y

Cuts at:      0      15     225 
   Size:         15     210
 
PmlI CAC'GTG

Cuts at:      0     144     225 
   Size:        144      81
 
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'

Cuts at:      0     190     222     225 
   Size:        190      32       3

  Fragments arranged by size: 

                 190      32       3
 
SacII CC_GC'GG

Cuts at:      0     116     225 
   Size:        116     109
 
Tth111I GACn'n_nGTC

Cuts at:      0      91     225 
   Size:         91     134
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0      54     225 
   Size:         54     171
 
XmnI GAAnn'nnTTC

Cuts at:      0     205     225 
   Size:        205      20
 

 Enzymes that do cut:

    AlwNI     BsaI    BsaAI    BstYI     BtgI     FalI    Hin4I     MslI
   MspA1I     NruI     NspI     PmlI     PpiI    SacII  Tth111I     XcmI
     XmnI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI     ApaI    ApaLI
     ApoI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BamHI
     BanI    BanII     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI
    BfrBI     BglI    BglII     BlpI Bme1580I    BmgBI     BmrI     BmtI
     BplI     BpmI   Bpu10I    BpuEI    BsaBI    BsaHI    BsaWI    BsaXI
    BseRI    BseYI     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BsmBI
 Bsp1286I    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI    BsrGI
   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I   Bsu36I
    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI     EaeI
     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FseI     FspI    FspAI    HaeII   HincII  HindIII     HpaI
     KasI     KpnI     MfeI     MluI     MmeI     MscI     NaeI     NarI
     NcoI     NdeI   NgoMIV     NheI     NotI     NsiI     PacI     PasI
     PciI    PflMI     PfoI     PmeI    PpuMI    PshAI     PsiI   PspOMI
    PspXI     PsrI     PstI     PvuI    PvuII    RsrII     SacI     SalI
    SanDI     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI
     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI
     SspI     StuI     StyI     SwaI    TaqII     TatI     TstI     XbaI
     XhoI     XmaI     ZraI