(Linear) (Six-base) MAPSORT of: KIBB3715.seq  Check: 4823  from: 1  to: 624



KIBB3715.orf

 With 182 enzymes: SgfI * 

                        July 18, 2007 13:37  ..

AatII G_ACGT'C

Cuts at:      0      82     332     624 
   Size:         82     250     292

  Fragments arranged by size: 

                 292     250      82
 
AflIII A'CryG_T

Cuts at:      0     504     624 
   Size:        504     120
 
AhdI GACnn_n'nnGTC

Cuts at:      0     150     624 
   Size:        150     474
 
ApoI r'AATT_y

Cuts at:      0      37     624 
   Size:         37     587
 
BanII G_rGCy'C

Cuts at:      0     596     624 
   Size:        596      28
 
BbsI GAAGACnn'nnnn_

Cuts at:      0      82     478     624 
   Size:         82     396     146

  Fragments arranged by size: 

                 396     146      82
 
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'

Cuts at:      0     481     515     624 
   Size:        481      34     109

  Fragments arranged by size: 

                 481     109      34
 
BmgBI CAC'GTC

Cuts at:      0     505     624 
   Size:        505     119
 
BsaI GGTCTCn'nnnn_

Cuts at:      0     454     624 
   Size:        454     170
 
BsaBI GATnn'nnATC

Cuts at:      0     409     568     624 
   Size:        409     159      56

  Fragments arranged by size: 

                 409     159      56
 
BsaHI Gr'CG_yC

Cuts at:      0      79     329     511     624 
   Size:         79     250     182     113

  Fragments arranged by size: 

                 250     182     113      79
 
BseRI GAGGAGnnnnnnnn_nn'

Cuts at:      0     430     624 
   Size:        430     194
 
BseYI C'CCAG_C

Cuts at:      0     238     624 
   Size:        238     386
 
BsmBI CGTCTCn'nnnn_

Cuts at:      0      86     624 
   Size:         86     538
 
Bsp1286I G_dGCh'C

Cuts at:      0     596     624 
   Size:        596      28
 
BssHII G'CGCG_C

Cuts at:      0     429     624 
   Size:        429     195
 
BstXI CCAn_nnnn'nTGG

Cuts at:      0     168     624 
   Size:        168     456
 
BtgI C'CryG_G

Cuts at:      0     257     624 
   Size:        257     367
 
BtsI GCAGTG_nn'

Cuts at:      0     354     624 
   Size:        354     270
 
EaeI y'GGCC_r

Cuts at:      0     599     624 
   Size:        599      25
 
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'

Cuts at:      0     136     168     179     211     624 
   Size:        136      32      11      32     413

  Fragments arranged by size: 

                 413     136      32      32      11
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     327     624 
   Size:        327     297
 
MslI CAynn'nnrTG

Cuts at:      0     367     624 
   Size:        367     257
 
MspA1I CmG'CkG

Cuts at:      0     208     624 
   Size:        208     416
 
PfoI T'CCnGG_A

Cuts at:      0     248     539     624 
   Size:        248     291      85

  Fragments arranged by size: 

                 291     248      85
 
PstI C_TGCA'G

Cuts at:      0     194     624 
   Size:        194     430
 
PvuII CAG'CTG

Cuts at:      0     208     624 
   Size:        208     416
 
SfcI C'TryA_G

Cuts at:      0     190     624 
   Size:        190     434
 
TatI w'GTAC_w

Cuts at:      0     164     624 
   Size:        164     460
 
Tth111I GACn'n_nGTC

Cuts at:      0     328     624 
   Size:        328     296
 
XcmI CCAnnnn_n'nnnnTGG

Cuts at:      0     133     264     624 
   Size:        133     131     360

  Fragments arranged by size: 

                 360     133     131
 
ZraI GAC'GTC

Cuts at:      0      80     330     624 
   Size:         80     250     294

  Fragments arranged by size: 

                 294     250      80
 

 Enzymes that do cut:

    AatII   AflIII     AhdI     ApoI    BanII     BbsI     BcgI    BmgBI
     BsaI    BsaBI    BsaHI    BseRI    BseYI    BsmBI Bsp1286I   BssHII
    BstXI     BtgI     BtsI     EaeI    Hin4I     MmeI     MslI   MspA1I
     PfoI     PstI    PvuII     SfcI     TatI  Tth111I     XcmI     ZraI

 Enzymes that do not cut: 

     AarI     AccI   Acc65I     AclI     AcuI     AfeI    AflII     AgeI
     AleI     AlfI     AloI    AlwNI     ApaI    ApaLI     AscI     AseI
    AsiSI     AvaI    AvrII     BaeI    BamHI     BanI     BbeI    BbvCI
    BciVI     BclI    BfrBI     BglI    BglII     BlpI Bme1580I     BmrI
     BmtI     BplI     BpmI   Bpu10I    BpuEI    BsaAI    BsaWI    BsaXI
     BsgI    BsiEI  BsiHKAI    BsiWI     BsmI    BspEI    BspHI    BspMI
    BsrBI    BsrDI    BsrFI    BsrGI    BssSI   BstAPI    BstBI   BstEII
    BstYI  BstZ17I   Bsu36I    BtgZI     ClaI    CspCI     DraI   DraIII
     DrdI     EagI     EarI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I
    EcoRI    EcoRV     FalI     FseI     FspI    FspAI    HaeII   HincII
  HindIII     HpaI     KasI     KpnI     MfeI     MluI     MscI     NaeI
     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI     NsiI
     NspI     PacI     PasI     PciI    PflMI     PmeI     PmlI     PpiI
    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI     PvuI    RsrII
     SacI    SacII     SalI    SanDI     SapI     SbfI     ScaI    SexAI
     SfiI     SfoI     SgfI    SgrAI     SmaI     SmlI    SnaBI     SpeI
     SphI     SrfI     SspI     StuI     StyI     SwaI    TaqII     TstI
     XbaI     XhoI     XmaI     XmnI