(Linear) (Six-base) MAPSORT of: KIBB3718.seq  Check: 6823  from: 1  to: 495



KIBB3718.orf

 With 182 enzymes: SgfI * 

                        April 24, 2009 13:02  ..

ApoI r'AATT_y

Cuts at:      0     379     495 
   Size:        379     116
 
BamHI G'GATC_C

Cuts at:      0     460     495 
   Size:        460      35
 
BanI G'GyrC_C

Cuts at:      0     312     495 
   Size:        312     183
 
BglII A'GATC_T

Cuts at:      0     161     423     495 
   Size:        161     262      72

  Fragments arranged by size: 

                 262     161      72
 
Bme1580I G_kGCm'C

Cuts at:      0     315     495 
   Size:        315     180
 
BsaBI GATnn'nnATC

Cuts at:      0     428     495 
   Size:        428      67
 
BsaXI ACnnnnnCTCCnnnnnnn_nnn'

Cuts at:      0       2      32     495 
   Size:          2      30     463

  Fragments arranged by size: 

                 463      30       2
 
BsgI GTGCAGnnnnnnnnnnnnnn_nn'

Cuts at:      0     143     495 
   Size:        143     352
 
BsmI GAATG_Cn'

Cuts at:      0     224     495 
   Size:        224     271
 
Bsp1286I G_dGCh'C

Cuts at:      0     315     495 
   Size:        315     180
 
BstYI r'GATC_y

Cuts at:      0     161     423     460     495 
   Size:        161     262      37      35

  Fragments arranged by size: 

                 262     161      37      35
 
Bsu36I CC'TnA_GG

Cuts at:      0       6     495 
   Size:          6     489
 
EarI CTCTTCn'nnn_

Cuts at:      0     284     495 
   Size:        284     211
 
HindIII A'AGCT_T

Cuts at:      0     261     495 
   Size:        261     234
 
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'

Cuts at:      0     150     495 
   Size:        150     345
 
StyI C'CwwG_G

Cuts at:      0     359     495 
   Size:        359     136
 
TstI CACnnnnnnTCCnnnnnnn_nnnnn'

Cuts at:      0     476     495 
   Size:        476      19
 

 Enzymes that do cut:

     ApoI    BamHI     BanI    BglII Bme1580I    BsaBI    BsaXI     BsgI
     BsmI Bsp1286I    BstYI   Bsu36I     EarI  HindIII     MmeI     StyI
     TstI

 Enzymes that do not cut: 

     AarI    AatII     AccI   Acc65I     AclI     AcuI     AfeI    AflII
   AflIII     AgeI     AhdI     AleI     AlfI     AloI    AlwNI     ApaI
    ApaLI     AscI     AseI    AsiSI     AvaI    AvrII     BaeI    BanII
     BbeI     BbsI    BbvCI     BcgI    BciVI     BclI    BfrBI     BglI
     BlpI    BmgBI     BmrI     BmtI     BplI     BpmI   Bpu10I    BpuEI
     BsaI    BsaAI    BsaHI    BsaWI    BseRI    BseYI    BsiEI  BsiHKAI
    BsiWI    BsmBI    BspEI    BspHI    BspMI    BsrBI    BsrDI    BsrFI
    BsrGI   BssHII    BssSI   BstAPI    BstBI   BstEII    BstXI  BstZ17I
     BtgI    BtgZI     BtsI     ClaI    CspCI     DraI   DraIII     DrdI
     EaeI     EagI     EciI  EcoICRI  Eco57MI    EcoNI EcoO109I    EcoRI
    EcoRV     FalI     FseI     FspI    FspAI    HaeII    Hin4I   HincII
     HpaI     KasI     KpnI     MfeI     MluI     MscI     MslI   MspA1I
     NaeI     NarI     NcoI     NdeI   NgoMIV     NheI     NotI     NruI
     NsiI     NspI     PacI     PasI     PciI    PflMI     PfoI     PmeI
     PmlI     PpiI    PpuMI    PshAI     PsiI   PspOMI    PspXI     PsrI
     PstI     PvuI    PvuII    RsrII     SacI    SacII     SalI    SanDI
     SapI     SbfI     ScaI    SexAI     SfcI     SfiI     SfoI     SgfI
    SgrAI     SmaI     SmlI    SnaBI     SpeI     SphI     SrfI     SspI
     StuI     SwaI    TaqII     TatI  Tth111I     XbaI     XcmI     XhoI
     XmaI     XmnI     ZraI