Restriction map for
pF1KB3720
(Linear) (Six-base) MAPSORT of: KIBB3720.seq Check: 4583 from: 1 to: 972
KIBB3720.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AleI CACnn'nnGTG
Cuts at: 0 164 745 972
Size: 164 581 227
Fragments arranged by size:
581 227 164
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 170 972
Size: 170 802
AlwNI CAG_nnn'CTG
Cuts at: 0 558 972
Size: 558 414
ApaI G_GGCC'C
Cuts at: 0 942 972
Size: 942 30
ApoI r'AATT_y
Cuts at: 0 376 972
Size: 376 596
AvaI C'yCGr_G
Cuts at: 0 635 972
Size: 635 337
AvrII C'CTAG_G
Cuts at: 0 958 972
Size: 958 14
BanI G'GyrC_C
Cuts at: 0 739 842 972
Size: 739 103 130
Fragments arranged by size:
739 130 103
BanII G_rGCy'C
Cuts at: 0 877 942 972
Size: 877 65 30
Fragments arranged by size:
877 65 30
BglI GCCn_nnn'nGGC
Cuts at: 0 67 712 972
Size: 67 645 260
Fragments arranged by size:
645 260 67
Bme1580I G_kGCm'C
Cuts at: 0 85 615 636 742 797 942 972
Size: 85 530 21 106 55 145 30
Fragments arranged by size:
530 145 106 85 55 30 21
BmgBI CAC'GTC
Cuts at: 0 854 972
Size: 854 118
BmrI ACTGGGnnnn_n'
Cuts at: 0 345 904 972
Size: 345 559 68
Fragments arranged by size:
559 345 68
BmtI G_CTAG'C
Cuts at: 0 257 667 972
Size: 257 410 305
Fragments arranged by size:
410 305 257
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 298 752 972
Size: 183 115 454 220
Fragments arranged by size:
454 220 183 115
BsaAI yAC'GTr
Cuts at: 0 621 972
Size: 621 351
BsaWI w'CCGG_w
Cuts at: 0 134 908 972
Size: 134 774 64
Fragments arranged by size:
774 134 64
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 469 499 972
Size: 469 30 473
Fragments arranged by size:
473 469 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 223 251 715 972
Size: 223 28 464 257
Fragments arranged by size:
464 257 223 28
BseYI C'CCAG_C
Cuts at: 0 544 562 594 935 972
Size: 544 18 32 341 37
Fragments arranged by size:
544 341 37 32 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 140 207 972
Size: 140 67 765
Fragments arranged by size:
765 140 67
BsmI GAATG_Cn'
Cuts at: 0 421 972
Size: 421 551
Bsp1286I G_dGCh'C
Cuts at: 0 85 615 636 742 797 877 942 972
Size: 85 530 21 106 55 80 65 30
Fragments arranged by size:
530 106 85 80 65 55 30 21
BspEI T'CCGG_A
Cuts at: 0 908 972
Size: 908 64
BsrBI CCG'CTC
Cuts at: 0 480 972
Size: 480 492
BsrDI GCAATG_nn'
Cuts at: 0 818 972
Size: 818 154
BssHII G'CGCG_C
Cuts at: 0 11 972
Size: 11 961
BstXI CCAn_nnnn'nTGG
Cuts at: 0 30 496 972
Size: 30 466 476
Fragments arranged by size:
476 466 30
BtgI C'CryG_G
Cuts at: 0 344 534 797 972
Size: 344 190 263 175
Fragments arranged by size:
344 263 190 175
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 438 972
Size: 438 534
BtsI GCAGTG_nn'
Cuts at: 0 219 972
Size: 219 753
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 183 298 752 972
Size: 183 115 454 220
Fragments arranged by size:
454 220 183 115
EcoO109I rG'GnC_Cy
Cuts at: 0 555 714 922 972
Size: 555 159 208 50
Fragments arranged by size:
555 208 159 50
FspI TGC'GCA
Cuts at: 0 289 972
Size: 289 683
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 183 215 491 523 972
Size: 183 32 276 32 449
Fragments arranged by size:
449 276 183 32 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 467 972
Size: 467 505
MslI CAynn'nnrTG
Cuts at: 0 164 469 745 972
Size: 164 305 276 227
Fragments arranged by size:
305 276 227 164
MspA1I CmG'CkG
Cuts at: 0 839 972
Size: 839 133
NcoI C'CATG_G
Cuts at: 0 534 972
Size: 534 438
NdeI CA'TA_TG
Cuts at: 0 696 809 972
Size: 696 113 163
Fragments arranged by size:
696 163 113
NheI G'CTAG_C
Cuts at: 0 253 663 972
Size: 253 410 309
Fragments arranged by size:
410 309 253
PflMI CCAn_nnn'nTGG
Cuts at: 0 366 972
Size: 366 606
PfoI T'CCnGG_A
Cuts at: 0 644 972
Size: 644 328
PpuMI rG'GwC_Cy
Cuts at: 0 922 972
Size: 922 50
PspOMI G'GGCC_C
Cuts at: 0 938 972
Size: 938 34
PstI C_TGCA'G
Cuts at: 0 554 677 972
Size: 554 123 295
Fragments arranged by size:
554 295 123
PvuII CAG'CTG
Cuts at: 0 839 972
Size: 839 133
SbfI CC_TGCA'GG
Cuts at: 0 554 972
Size: 554 418
ScaI AGT'ACT
Cuts at: 0 295 972
Size: 295 677
SexAI A'CCwGG_T
Cuts at: 0 107 972
Size: 107 865
SfcI C'TryA_G
Cuts at: 0 550 673 834 972
Size: 550 123 161 138
Fragments arranged by size:
550 161 138 123
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 712 972
Size: 712 260
StyI C'CwwG_G
Cuts at: 0 381 534 743 958 972
Size: 381 153 209 215 14
Fragments arranged by size:
381 215 209 153 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 885 972
Size: 885 87
TatI w'GTAC_w
Cuts at: 0 171 293 972
Size: 171 122 679
Fragments arranged by size:
679 171 122
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 325 357 469 501 972
Size: 325 32 112 32 471
Fragments arranged by size:
471 325 112 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 891 972
Size: 891 81
Enzymes that do cut:
AleI AlfI AlwNI ApaI ApoI AvaI AvrII BanI
BanII BglI Bme1580I BmgBI BmrI BmtI BpmI BsaAI
BsaWI BsaXI BseRI BseYI BsgI BsmI Bsp1286I BspEI
BsrBI BsrDI BssHII BstXI BtgI BtgZI BtsI Eco57MI
EcoO109I FspI Hin4I MmeI MslI MspA1I NcoI NdeI
NheI PflMI PfoI PpuMI PspOMI PstI PvuII SbfI
ScaI SexAI SfcI SfiI StyI TaqII TatI TstI
XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AloI ApaLI AscI AseI AsiSI
BaeI BamHI BbeI BbsI BbvCI BcgI BciVI BclI
BfrBI BglII BlpI BplI Bpu10I BpuEI BsaI BsaBI
BsaHI BsiEI BsiHKAI BsiWI BsmBI BspHI BspMI BsrFI
BsrGI BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
ClaI CspCI DraI DraIII DrdI EaeI EagI EarI
EciI EcoICRI EcoNI EcoRI EcoRV FalI FseI FspAI
HaeII HincII HindIII HpaI KasI KpnI MfeI MluI
MscI NaeI NarI NgoMIV NotI NruI NsiI NspI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SacII SalI SanDI
SapI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI
SphI SrfI SspI StuI SwaI Tth111I XbaI XhoI
XmaI XmnI ZraI