Restriction map for
pF1KB3725
(Linear) (Six-base) MAPSORT of: KIBB3725.seq Check: 6490 from: 1 to: 4125
KIBB3725.orf
With 182 enzymes: SgfI *
February 28, 2007 11:24 ..
AccI GT'mk_AC
Cuts at: 0 668 1794 2081 4125
Size: 668 1126 287 2044
Fragments arranged by size:
2044 1126 668 287
Acc65I G'GTAC_C
Cuts at: 0 1526 2521 2904 4125
Size: 1526 995 383 1221
Fragments arranged by size:
1526 1221 995 383
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1142 1531 2215 3125 3254 3874 3947
Size: 604 538 389 684 910 129 620 73
Cuts at: 3947 4019 4125
Size: 72 106
Fragments arranged by size:
910 684 620 604 538 389 129 106
73 72
AflII C'TTAA_G
Cuts at: 0 3859 4125
Size: 3859 266
AflIII A'CryG_T
Cuts at: 0 4036 4125
Size: 4036 89
AgeI A'CCGG_T
Cuts at: 0 3716 4125
Size: 3716 409
AhdI GACnn_n'nnGTC
Cuts at: 0 2592 4125
Size: 2592 1533
AlwNI CAG_nnn'CTG
Cuts at: 0 161 582 1921 2163 2220 2767 4125
Size: 161 421 1339 242 57 547 1358
Fragments arranged by size:
1358 1339 547 421 242 161 57
ApaLI G'TGCA_C
Cuts at: 0 615 4080 4125
Size: 615 3465 45
Fragments arranged by size:
3465 615 45
ApoI r'AATT_y
Cuts at: 0 893 1806 1846 3038 3072 3289 3878 3906
Size: 893 913 40 1192 34 217 589 28
Cuts at: 3906 4125
Size: 219
Fragments arranged by size:
1192 913 893 589 219 217 40 34
28
AseI AT'TA_AT
Cuts at: 0 1298 1684 2432 4125
Size: 1298 386 748 1693
Fragments arranged by size:
1693 1298 748 386
AvaI C'yCGr_G
Cuts at: 0 54 73 216 230 4125
Size: 54 19 143 14 3895
Fragments arranged by size:
3895 143 54 19 14
BamHI G'GATC_C
Cuts at: 0 3170 4125
Size: 3170 955
BanI G'GyrC_C
Cuts at: 0 370 1526 2521 2538 2904 4125
Size: 370 1156 995 17 366 1221
Fragments arranged by size:
1221 1156 995 370 366 17
BanII G_rGCy'C
Cuts at: 0 710 1355 2227 4125
Size: 710 645 872 1898
Fragments arranged by size:
1898 872 710 645
BbeI G_GCGC'C
Cuts at: 0 374 4125
Size: 374 3751
BbsI GAAGACnn'nnnn_
Cuts at: 0 1375 1519 3188 3578 3633 3980 4125
Size: 1375 144 1669 390 55 347 145
Fragments arranged by size:
1669 1375 390 347 145 144 55
BbvCI CC'TCA_GC
Cuts at: 0 3133 4125
Size: 3133 992
BclI T'GATC_A
Cuts at: 0 287 3564 4125
Size: 287 3277 561
Fragments arranged by size:
3277 561 287
BfrBI ATG'CAT
Cuts at: 0 1066 4125
Size: 1066 3059
BglII A'GATC_T
Cuts at: 0 2456 4125
Size: 2456 1669
Bme1580I G_kGCm'C
Cuts at: 0 619 4084 4125
Size: 619 3465 41
Fragments arranged by size:
3465 619 41
BmrI ACTGGGnnnn_n'
Cuts at: 0 629 1434 2659 4125
Size: 629 805 1225 1466
Fragments arranged by size:
1466 1225 805 629
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 2964 4125
Size: 2964 1161
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1399 2032 2294 2512 3367 4125
Size: 1399 633 262 218 855 758
Fragments arranged by size:
1399 855 758 633 262 218
Bpu10I CC'TnA_GC
Cuts at: 0 2846 3133 3224 4125
Size: 2846 287 91 901
Fragments arranged by size:
2846 901 287 91
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1516 2118 2437 4077 4125
Size: 1516 602 319 1640 48
Fragments arranged by size:
1640 1516 602 319 48
BsaI GGTCTCn'nnnn_
Cuts at: 0 1229 2600 4125
Size: 1229 1371 1525
Fragments arranged by size:
1525 1371 1229
BsaAI yAC'GTr
Cuts at: 0 1770 4039 4125
Size: 1770 2269 86
Fragments arranged by size:
2269 1770 86
BsaHI Gr'CG_yC
Cuts at: 0 371 4125
Size: 371 3754
BsaWI w'CCGG_w
Cuts at: 0 1355 3716 4125
Size: 1355 2361 409
Fragments arranged by size:
2361 1355 409
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 3791 3821 4125
Size: 3791 30 304
Fragments arranged by size:
3791 304 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 113 139 204 1303 3083 4125
Size: 113 26 65 1099 1780 1042
Fragments arranged by size:
1780 1099 1042 113 65 26
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 48 4125
Size: 48 4077
BsiHKAI G_wGCw'C
Cuts at: 0 7 131 619 710 1355 3093 3676 3714
Size: 7 124 488 91 645 1738 583 38
Cuts at: 3714 4084 4125
Size: 370 41
Fragments arranged by size:
1738 645 583 488 370 124 91 41
38 7
BsmI GAATG_Cn'
Cuts at: 0 2737 3059 4125
Size: 2737 322 1066
Fragments arranged by size:
2737 1066 322
Bsp1286I G_dGCh'C
Cuts at: 0 7 131 619 710 1355 2227 3093 3676
Size: 7 124 488 91 645 872 866 583
Cuts at: 3676 3714 4084 4125
Size: 38 370 41
Fragments arranged by size:
872 866 645 583 488 370 124 91
41 38 7
BspEI T'CCGG_A
Cuts at: 0 1355 4125
Size: 1355 2770
BspHI T'CATG_A
Cuts at: 0 689 3696 4125
Size: 689 3007 429
Fragments arranged by size:
3007 689 429
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 2077 2256 4125
Size: 2077 179 1869
Fragments arranged by size:
2077 1869 179
BsrBI CCG'CTC
Cuts at: 0 103 4125
Size: 103 4022
BsrDI GCAATG_nn'
Cuts at: 0 2179 3272 4125
Size: 2179 1093 853
Fragments arranged by size:
2179 1093 853
BsrFI r'CCGG_y
Cuts at: 0 2901 3716 3986 4125
Size: 2901 815 270 139
Fragments arranged by size:
2901 815 270 139
BsrGI T'GTAC_A
Cuts at: 0 1912 2605 3729 4125
Size: 1912 693 1124 396
Fragments arranged by size:
1912 1124 693 396
BstAPI GCAn_nnn'nTGC
Cuts at: 0 135 345 451 4125
Size: 135 210 106 3674
Fragments arranged by size:
3674 210 135 106
BstBI TT'CG_AA
Cuts at: 0 4110 4125
Size: 4110 15
BstXI CCAn_nnnn'nTGG
Cuts at: 0 426 4125
Size: 426 3699
BstYI r'GATC_y
Cuts at: 0 2456 2705 2763 3170 3298 4125
Size: 2456 249 58 407 128 827
Fragments arranged by size:
2456 827 407 249 128 58
BstZ17I GTA'TAC
Cuts at: 0 1795 4125
Size: 1795 2330
Bsu36I CC'TnA_GG
Cuts at: 0 3632 3663 4125
Size: 3632 31 462
Fragments arranged by size:
3632 462 31
BtgI C'CryG_G
Cuts at: 0 272 1130 1239 3366 4125
Size: 272 858 109 2127 759
Fragments arranged by size:
2127 858 759 272 109
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 485 4125
Size: 485 3640
BtsI GCAGTG_nn'
Cuts at: 0 228 2943 4125
Size: 228 2715 1182
Fragments arranged by size:
2715 1182 228
DraI TTT'AAA
Cuts at: 0 1506 2691 2813 4125
Size: 1506 1185 122 1312
Fragments arranged by size:
1506 1312 1185 122
DraIII CAC_nnn'GTG
Cuts at: 0 1050 4088 4125
Size: 1050 3038 37
Fragments arranged by size:
3038 1050 37
EaeI y'GGCC_r
Cuts at: 0 206 320 530 1598 2621 4125
Size: 206 114 210 1068 1023 1504
Fragments arranged by size:
1504 1068 1023 210 206 114
EarI CTCTTCn'nnn_
Cuts at: 0 1824 1958 4125
Size: 1824 134 2167
Fragments arranged by size:
2167 1824 134
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 4125
Size: 28 4097
EcoICRI GAG'CTC
Cuts at: 0 708 1353 4125
Size: 708 645 2772
Fragments arranged by size:
2772 708 645
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 604 1142 1399 1531 2032 2215 2294 2512
Size: 604 538 257 132 501 183 79 218
Cuts at: 2512 3125 3254 3367 3874 3947 4019 4125
Size: 613 129 113 507 73 72 106
Fragments arranged by size:
613 604 538 507 501 257 218 183
132 129 113 106 79 73 72
EcoNI CCTnn'n_nnAGG
Cuts at: 0 1639 4125
Size: 1639 2486
EcoO109I rG'GnC_Cy
Cuts at: 0 333 2268 4125
Size: 333 1935 1857
Fragments arranged by size:
1935 1857 333
EcoRI G'AATT_C
Cuts at: 0 893 3038 4125
Size: 893 2145 1087
Fragments arranged by size:
2145 1087 893
EcoRV GAT'ATC
Cuts at: 0 969 4125
Size: 969 3156
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1419 3826 4125
Size: 1419 2407 299
Fragments arranged by size:
2407 1419 299
HaeII r_GCGC'y
Cuts at: 0 179 374 4125
Size: 179 195 3751
Fragments arranged by size:
3751 195 179
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 547 579 1021 1053 2578 2610 2704 2736
Size: 547 32 442 32 1525 32 94 32
Cuts at: 2736 2974 3006 4125
Size: 238 32 1119
Fragments arranged by size:
1525 1119 547 442 238 94 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 701 4125
Size: 701 3424
HindIII A'AGCT_T
Cuts at: 0 1498 2848 3856 4125
Size: 1498 1350 1008 269
Fragments arranged by size:
1498 1350 1008 269
KasI G'GCGC_C
Cuts at: 0 370 4125
Size: 370 3755
KpnI G_GTAC'C
Cuts at: 0 1530 2525 2908 4125
Size: 1530 995 383 1217
Fragments arranged by size:
1530 1217 995 383
MfeI C'AATT_G
Cuts at: 0 2336 4125
Size: 2336 1789
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1413 4125
Size: 1413 2712
MscI TGG'CCA
Cuts at: 0 532 1600 2623 4125
Size: 532 1068 1023 1502
Fragments arranged by size:
1502 1068 1023 532
MslI CAynn'nnrTG
Cuts at: 0 1046 1521 2617 4125
Size: 1046 475 1096 1508
Fragments arranged by size:
1508 1096 1046 475
MspA1I CmG'CkG
Cuts at: 0 135 181 880 1324 2866 4125
Size: 135 46 699 444 1542 1259
Fragments arranged by size:
1542 1259 699 444 135 46
NarI GG'CG_CC
Cuts at: 0 371 4125
Size: 371 3754
NcoI C'CATG_G
Cuts at: 0 1130 1239 3366 4125
Size: 1130 109 2127 759
Fragments arranged by size:
2127 1130 759 109
NdeI CA'TA_TG
Cuts at: 0 282 4125
Size: 282 3843
NsiI A_TGCA'T
Cuts at: 0 1068 4125
Size: 1068 3057
PflMI CCAn_nnn'nTGG
Cuts at: 0 2220 2535 4125
Size: 2220 315 1590
Fragments arranged by size:
2220 1590 315
PfoI T'CCnGG_A
Cuts at: 0 815 971 4125
Size: 815 156 3154
Fragments arranged by size:
3154 815 156
PmlI CAC'GTG
Cuts at: 0 1770 4039 4125
Size: 1770 2269 86
Fragments arranged by size:
2269 1770 86
PpuMI rG'GwC_Cy
Cuts at: 0 2268 4125
Size: 2268 1857
PspXI vC'TCGA_Gb
Cuts at: 0 216 4125
Size: 216 3909
PstI C_TGCA'G
Cuts at: 0 91 341 578 2727 4125
Size: 91 250 237 2149 1398
Fragments arranged by size:
2149 1398 250 237 91
PvuII CAG'CTG
Cuts at: 0 135 880 1324 2866 4125
Size: 135 745 444 1542 1259
Fragments arranged by size:
1542 1259 745 444 135
SacI G_AGCT'C
Cuts at: 0 710 1355 4125
Size: 710 645 2770
Fragments arranged by size:
2770 710 645
ScaI AGT'ACT
Cuts at: 0 811 4125
Size: 811 3314
SfcI C'TryA_G
Cuts at: 0 87 337 574 960 1285 2439 2723 4125
Size: 87 250 237 386 325 1154 284 1402
Fragments arranged by size:
1402 1154 386 325 284 250 237 87
SfoI GGC'GCC
Cuts at: 0 372 4125
Size: 372 3753
SmlI C'TyrA_G
Cuts at: 0 216 1531 2097 2452 3859 4056 4125
Size: 216 1315 566 355 1407 197 69
Fragments arranged by size:
1407 1315 566 355 216 197 69
SspI AAT'ATT
Cuts at: 0 1688 2208 4125
Size: 1688 520 1917
Fragments arranged by size:
1917 1688 520
StyI C'CwwG_G
Cuts at: 0 303 504 1130 1239 2732 3366 4125
Size: 303 201 626 109 1493 634 759
Fragments arranged by size:
1493 759 634 626 303 201 109
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 3323 4125
Size: 3323 802
TatI w'GTAC_w
Cuts at: 0 809 1912 2503 2605 3729 4125
Size: 809 1103 591 102 1124 396
Fragments arranged by size:
1124 1103 809 591 396 102
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 621 653 1797 1829 3129 3161 4125
Size: 621 32 1144 32 1300 32 964
Fragments arranged by size:
1300 1144 964 621 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 2047 4125
Size: 2047 2078
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 3507 3765 4125
Size: 3507 258 360
Fragments arranged by size:
3507 360 258
XhoI C'TCGA_G
Cuts at: 0 216 4125
Size: 216 3909
XmnI GAAnn'nnTTC
Cuts at: 0 2242 2283 3779 4125
Size: 2242 41 1496 346
Fragments arranged by size:
2242 1496 346 41
Enzymes that do cut:
AccI Acc65I AcuI AflII AflIII AgeI AhdI AlwNI
ApaLI ApoI AseI AvaI BamHI BanI BanII BbeI
BbsI BbvCI BclI BfrBI BglII Bme1580I BmrI BplI
BpmI Bpu10I BpuEI BsaI BsaAI BsaHI BsaWI BsaXI
BseRI BsgI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BstAPI BstBI BstXI BstYI
BstZ17I Bsu36I BtgI BtgZI BtsI DraI DraIII EaeI
EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV
FalI HaeII Hin4I HincII HindIII KasI KpnI MfeI
MmeI MscI MslI MspA1I NarI NcoI NdeI NsiI
PflMI PfoI PmlI PpuMI PspXI PstI PvuII SacI
ScaI SfcI SfoI SmlI SspI StyI TaqII TatI
TstI Tth111I XcmI XhoI XmnI
Enzymes that do not cut:
AarI AatII AclI AfeI AleI AlfI AloI ApaI
AscI AsiSI AvrII BaeI BcgI BciVI BglI BlpI
BmgBI BmtI BsaBI BseYI BsiEI BsiWI BsmBI BssHII
BssSI BstEII ClaI CspCI DrdI EagI FseI FspI
FspAI HpaI MluI NaeI NgoMIV NheI NotI NruI
NspI PacI PasI PciI PmeI PpiI PshAI PsiI
PspOMI PsrI PvuI RsrII SacII SalI SanDI SapI
SbfI SexAI SfiI SgfI SgrAI SmaI SnaBI SpeI
SphI SrfI StuI SwaI XbaI XmaI ZraI