Restriction map for
pF1KB3747
(Linear) (Six-base) MAPSORT of: KIBB3747.seq Check: 7930 from: 1 to: 978
KIBB3747.orf
With 182 enzymes: SgfI *
March 3, 2008 10:56 ..
AflIII A'CryG_T
Cuts at: 0 371 978
Size: 371 607
AhdI GACnn_n'nnGTC
Cuts at: 0 468 978
Size: 468 510
AleI CACnn'nnGTG
Cuts at: 0 814 978
Size: 814 164
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 614 646 978
Size: 614 32 332
Fragments arranged by size:
614 332 32
ApoI r'AATT_y
Cuts at: 0 60 978
Size: 60 918
AvaI C'yCGr_G
Cuts at: 0 291 978
Size: 291 687
BamHI G'GATC_C
Cuts at: 0 773 978
Size: 773 205
BanI G'GyrC_C
Cuts at: 0 802 978
Size: 802 176
BanII G_rGCy'C
Cuts at: 0 298 338 533 692 978
Size: 298 40 195 159 286
Fragments arranged by size:
298 286 195 159 40
BbsI GAAGACnn'nnnn_
Cuts at: 0 23 38 374 978
Size: 23 15 336 604
Fragments arranged by size:
604 336 23 15
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 569 603 978
Size: 569 34 375
Fragments arranged by size:
569 375 34
BclI T'GATC_A
Cuts at: 0 305 978
Size: 305 673
BglI GCCn_nnn'nGGC
Cuts at: 0 898 917 978
Size: 898 19 61
Fragments arranged by size:
898 61 19
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 978
Size: 241 737
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 270 978
Size: 270 708
BsaAI yAC'GTr
Cuts at: 0 204 978
Size: 204 774
BsaHI Gr'CG_yC
Cuts at: 0 314 671 698 978
Size: 314 357 27 280
Fragments arranged by size:
357 314 280 27
BsaWI w'CCGG_w
Cuts at: 0 455 978
Size: 455 523
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 614 644 706 736 978
Size: 614 30 62 30 242
Fragments arranged by size:
614 242 62 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 574 711 978
Size: 574 137 267
Fragments arranged by size:
574 267 137
BsiEI CG_ry'CG
Cuts at: 0 472 978
Size: 472 506
BsiHKAI G_wGCw'C
Cuts at: 0 298 978
Size: 298 680
Bsp1286I G_dGCh'C
Cuts at: 0 298 338 533 692 978
Size: 298 40 195 159 286
Fragments arranged by size:
298 286 195 159 40
BsrBI CCG'CTC
Cuts at: 0 686 978
Size: 686 292
BsrGI T'GTAC_A
Cuts at: 0 323 978
Size: 323 655
BssSI C'ACGA_G
Cuts at: 0 412 978
Size: 412 566
BstYI r'GATC_y
Cuts at: 0 773 978
Size: 773 205
BtgI C'CryG_G
Cuts at: 0 340 665 978
Size: 340 325 313
Fragments arranged by size:
340 325 313
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 910 978
Size: 910 68
EaeI y'GGCC_r
Cuts at: 0 26 899 978
Size: 26 873 79
Fragments arranged by size:
873 79 26
EarI CTCTTCn'nnn_
Cuts at: 0 476 978
Size: 476 502
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 405 978
Size: 405 573
EcoICRI GAG'CTC
Cuts at: 0 296 978
Size: 296 682
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 241 978
Size: 241 737
EcoNI CCTnn'n_nnAGG
Cuts at: 0 754 978
Size: 754 224
EcoO109I rG'GnC_Cy
Cuts at: 0 408 625 978
Size: 408 217 353
Fragments arranged by size:
408 353 217
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 361 393 454 486 978
Size: 361 32 61 32 492
Fragments arranged by size:
492 361 61 32 32
HincII GTy'rAC
Cuts at: 0 72 702 978
Size: 72 630 276
Fragments arranged by size:
630 276 72
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 452 978
Size: 452 526
MscI TGG'CCA
Cuts at: 0 901 978
Size: 901 77
MslI CAynn'nnrTG
Cuts at: 0 274 538 814 978
Size: 274 264 276 164
Fragments arranged by size:
276 274 264 164
MspA1I CmG'CkG
Cuts at: 0 332 342 594 978
Size: 332 10 252 384
Fragments arranged by size:
384 332 252 10
NcoI C'CATG_G
Cuts at: 0 665 978
Size: 665 313
NspI r_CATG'y
Cuts at: 0 375 978
Size: 375 603
PciI A'CATG_T
Cuts at: 0 371 978
Size: 371 607
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 614 646 978
Size: 614 32 332
Fragments arranged by size:
614 332 32
PpuMI rG'GwC_Cy
Cuts at: 0 408 978
Size: 408 570
PstI C_TGCA'G
Cuts at: 0 40 978
Size: 40 938
PvuII CAG'CTG
Cuts at: 0 594 978
Size: 594 384
SacI G_AGCT'C
Cuts at: 0 298 978
Size: 298 680
SacII CC_GC'GG
Cuts at: 0 343 978
Size: 343 635
SfcI C'TryA_G
Cuts at: 0 36 425 978
Size: 36 389 553
Fragments arranged by size:
553 389 36
SmlI C'TyrA_G
Cuts at: 0 249 978
Size: 249 729
StyI C'CwwG_G
Cuts at: 0 665 978
Size: 665 313
TatI w'GTAC_w
Cuts at: 0 323 978
Size: 323 655
Enzymes that do cut:
AflIII AhdI AleI AloI ApoI AvaI BamHI BanI
BanII BbsI BcgI BclI BglI BpmI BpuEI BsaAI
BsaHI BsaWI BsaXI BseRI BsiEI BsiHKAI Bsp1286I BsrBI
BsrGI BssSI BstYI BtgI BtgZI EaeI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I Hin4I HincII MmeI MscI
MslI MspA1I NcoI NspI PciI PpiI PpuMI PstI
PvuII SacI SacII SfcI SmlI StyI TatI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AgeI AlfI AlwNI ApaI ApaLI AscI AseI AsiSI
AvrII BaeI BbeI BbvCI BciVI BfrBI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI Bpu10I BsaI BsaBI
BseYI BsgI BsiWI BsmI BsmBI BspEI BspHI BspMI
BsrDI BsrFI BssHII BstAPI BstBI BstEII BstXI BstZ17I
Bsu36I BtsI ClaI CspCI DraI DraIII DrdI EagI
EcoRI EcoRV FalI FseI FspI FspAI HaeII HindIII
HpaI KasI KpnI MfeI MluI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI PacI PasI PflMI
PfoI PmeI PmlI PshAI PsiI PspOMI PspXI PsrI
PvuI RsrII SalI SanDI SapI SbfI ScaI SexAI
SfiI SfoI SgfI SgrAI SmaI SnaBI SpeI SphI
SrfI SspI StuI SwaI TaqII TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI